LOCUS       pCAGGS-Flag-NACHT 5331 bp dsDNACircular
DEFINITION  LMBP 5236.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     misc_feature    44..553
                     /label=NACHT
     terminator      576..667
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
                     /label=ß-globin\polyA
     misc_feature    668..1024
                     /note="3' flanking region of the rabbit ß-globin gene"
                     /label=3FR
     rep_origin      1231..1431
                     /note="SV40 fragment containing bidirectional ori"
                     /label=SV40\ori
     rep_origin      complement(1621..2022)
                     /note="origin plasmid"
                     /label=pMB1\ori
     terminator      1432..1506
                     /note="polyA signal of the SV40 early region"
                     /label=SV40\polyA\early
     terminator      complement(1432..1506)
                     /note="polyA signal of the SV40 late region"
                     /label=SV40\polyA\late
     promoter        3563..3940
                     /note="hCMV-IE enhancer"
                     /label=hCMV\enhancer
     promoter        3946..5287
                     /note="ß-actin/ß-globin hybrid promoter"
                     /label=AG\promoter
     promoter        complement(1102..1184)
                     /label=lac\promoter
     misc_difference 5303..5326
                     /label=FLAG\tag
     CDS             complement(2563..3423)
                     /note="antibiotic resistance gene"
                     /label=AMP
     intron          4314..5239
                     /label=intron
ORIGIN
        1 catcagagac ttatttggcc caggcctgga tacccaagaa cctcgcatag tcatactgca 
       61 gggggctgct ggaattggga agtcaacact ggccaggcag gtgaaggaag cctgggggag 
      121 aggccagctg tatggggacc gcttccagca tgtcttctac ttcagctgca gagagctggc 
      181 ccagtccaag gtggtgagtc tcgctgagct catcggaaaa gatgggacag ccactccggc 
      241 tcccattaga cagatcctgt ctaggccaga gcggctgctc ttcatcctcg atggtgtaga 
      301 tgagccagga tgggtcttgc aggagccgag ttctgagctc tgtctgcact ggagccagcc 
      361 acagccggcg gatgcactgc tgggcagttt gctggggaaa actatacttc ccgaggcatc 
      421 cttcctgatc acggctcgga ccacagctct gcagaacctc attccttctt tggagcaggc 
      481 acgttgggta gaggtcctgg ggttctctga gtccagcagg aaggaatatt tctacagata 
      541 tttcacagat gaaaggcaag caattagagc ctgaagatct ttttccctct gccaaaaatt 
      601 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt 
      661 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg 
      721 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata 
      781 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc 
      841 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat 
      901 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag 
      961 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga 
     1021 gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga 
     1081 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 
     1141 tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
     1201 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc 
     1261 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     1321 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 
     1381 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt 
     1441 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     1501 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     1561 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
     1621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     1681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata acgcaggaaa 
     1741 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     1801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     1861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     1921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     1981 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     2041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     2101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     2161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     2221 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     2281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     2341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     2401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     2461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     2521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     2581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     2641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     2701 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     2761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     2821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     2881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     2941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     3001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     3061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     3121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     3181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     3241 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     3301 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     3361 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     3421 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     3481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     3541 aaaagtgcca cctgggtcga tcgacattga ttattgacta gttattaata gtaatcaatt 
     3601 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 
     3661 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 
     3721 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 
     3781 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 
     3841 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
     3901 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg 
     3961 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt 
     4021 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg 
     4081 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag 
     4141 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 
     4201 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc 
     4261 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 
     4321 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt 
     4381 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg 
     4441 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg 
     4501 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg 
     4561 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac 
     4621 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc 
     4681 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt 
     4741 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag 
     4801 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg 
     4861 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 
     4921 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 
     4981 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 
     5041 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 
     5101 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 
     5161 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 
     5221 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 
     5281 tggcaaagaa ttcctcgaga tggactacaa ggacgacgat gacaagggta c 
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