LOCUS       pCAGGS-E-hPTBdelnls        6373 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5259.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5259
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|448387411|
COMMENT     VNTNAME|pCAGGS-E-hPTBdelnls|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hPTBdelnls" 0 6373 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "EcoRI" "NotI" "XhoI" "NcoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6373 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1578 0 6372 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 52 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1572 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 7 0 223 0 772 0 2401 0 
COMMENT            5017 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "E-tag" 22 0 0 11 49 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "lac promoter" 30 0 1 2184 2266 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 33 0 1 2703 3104 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "ampicillin" 4 0 1 3645 4505 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4641 5018 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "AG promoter" 29 0 0 5024 6365 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "hPTBdelnls" 4 0 0 59 1570 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "59..1570") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 2408 2483 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2408..2483") 
COMMENT        #12=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1658 1749 0 
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COMMENT        #13=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2514 2588 0 
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COMMENT        #15=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1750 2106 0 
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COMMENT        #16=(CFSignal (CObList) "intron AG promoter" 15 0 0 5393 6317 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-hPTBdelnls'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 06/05/07 04:03" 1) 
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COMMENT                          "Last Modified: 10/24/07 10:12" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                          "Modification Date in the Original DB: 2-FEB-2007" 1))) 
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COMMENT                                        "Human polypyrimidine tract binding protein cDNA, delta nuclear localization seq. " 
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COMMENT                                        "intron of the chicken \337-actin/rabbit \337-globin hybrid promoter" 
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COMMENT                                 "hCMV-IE enhancer") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     misc_marker     11..49
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     rep_origin      2408..2483
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        complement(2184..2266)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="Escherichia coli lac operon promoter"
     terminator      1658..1749
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     rep_origin      complement(2703..3104)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      2514..2588
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2514..2588)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     misc_feature    1750..2106
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        5024..6365
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="Chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4641..5018
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     intron          5393..6317
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
     CDS             complement(3645..4505)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     CDS             59..1570
                     /vntifkey="4"
                     /label=hPTBdelnls
                     /note="Human polypyrimidine tract binding protein cDNA, delta nuclear localization seq."
BASE COUNT     1332 a      1810 c      1771 g      1460 t 
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat 
       61 ggtcccctct agagtgatcc acatccggaa gctccccatc gacgtcacgg agggggaagt 
      121 catctccctg gggctgccct ttgggaaggt caccaacctc ctgatgctga aggggaaaaa 
      181 ccaggccttc atcgagatga acacggagga ggctgccaac accatggtga actactacac 
      241 ctcggtgacc cctgtgctgc gcggccagcc catctacatc cagttctcca accacaagga 
      301 gctgaagacc gacagctctc ccaaccaggc gcgggcccag gcggccctgc aggcggtgaa 
      361 ctcggtccag tcggggaacc tggccttggc tgcctcggcg gcggccgtgg acgcagggat 
      421 ggcgatggcc gggcagagcc ccgtgctcag gatcatcgtg gagaacctct tctaccctgt 
      481 gaccctggat gtgctgcacc agattttctc caagttcggc acagtgttga agatcatcac 
      541 cttcaccaag aacaaccagt tccaggccct gctgcagtat gcggaccccg tgagcgccca 
      601 gcacgccaag ctgtcgctgg acgggcagaa catctacaac gcctgctgca cgctgcgcat 
      661 cgacttttcc aagctcacca gcctcaacgt caagtacaac aatgacaaga gccgtgacta 
      721 cacacgccca gacctgcctt ccggggacag ccagccctcg ctggaccaga ccatggccgc 
      781 ggccttcggt gcacctggta taatctcagc ctctccgtat gcaggagctg gtttccctcc 
      841 cacctttgcc attcctcaag ctgcaggcct ttccgttccg aacgtccacg gcgccctggc 
      901 ccccctggcc atcccctcgg cggcggcggc agctgcggcg gcaggtcgga tcgccatccc 
      961 gggcctggcg ggggcaggaa attctgtatt gctggtcagc aacctcaacc cagagagagt 
     1021 cacaccccaa agcctcttta ttcttttcgg cgtctacggt gacgtgcagc gcgtgaagat 
     1081 cctgttcaat aagaaggaga acgccctagt gcagatggcg gacggcaacc aggcccagct 
     1141 ggccatgagc cacctgaacg ggcacaagct gcacgggaag cccatccgca tcacgctctc 
     1201 gaagcaccag aacgtgcagc tgccccgcga gggccaggag gaccagggcc tgaccaagga 
     1261 ctacggcaac tcacccctgc accgcttcaa gaagccgggc tccaagaact tccagaacat 
     1321 attcccgccc tcggccacgc tgcacctctc caacatcccg ccctcagtct ccgaggagga 
     1381 tctcaaggtc ctgttttcca gcaatggggg cgtcgtcaaa ggattcaagt tcttccagaa 
     1441 ggaccgcaag atggcactga tccagatggg ctccgtggag gaggcggtcc aggccctcat 
     1501 tgacctgcac aaccacgacc tcggggagaa ccaccacctg cgggtctcct tctccaagtc 
     1561 caccatctag ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc 
     1621 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa 
     1681 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 
     1741 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 
     1801 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca 
     1861 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc 
     1921 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 
     1981 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 
     2041 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 
     2101 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     2161 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 
     2221 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
     2281 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc 
     2341 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
     2401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
     2461 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt 
     2521 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     2581 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     2641 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 
     2701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     2761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga 
     2821 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     2881 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     2941 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     3001 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     3061 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     3121 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     3181 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     3241 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     3301 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
     3361 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     3421 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     3481 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     3541 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     3601 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     3661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     3721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     3781 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     3841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     3901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     3961 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     4021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     4081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     4141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     4201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     4261 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     4321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     4381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     4441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     4501 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     4561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     4621 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac 
     4681 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 
     4741 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 
     4801 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 
     4861 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 
     4921 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 
     4981 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 
     5041 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 
     5101 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc 
     5161 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag 
     5221 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa 
     5281 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc 
     5341 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 
     5401 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 
     5461 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 
     5521 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 
     5581 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 
     5641 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 
     5701 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 
     5761 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 
     5821 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 
     5881 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg 
     5941 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat 
     6001 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat 
     6061 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca 
     6121 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc 
     6181 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg 
     6241 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct 
     6301 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg 
     6361 gcaaagaatt gaa 
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