ID pCAGGS-E-hPTBdelnls standard; circular DNA; ; 6373 BP. DE LMBP 5259. CC Accession number: LMBP 5259 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 24-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 11..49 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT rep_origin 2408..2483 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter complement(2184..2266) FT /vntifkey="30" FT /label=lac\promoter FT /note="Escherichia coli lac operon promoter" FT terminator 1658..1749 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT rep_origin complement(2703..3104) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT terminator 2514..2588 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2514..2588) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT misc_feature 1750..2106 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter 5024..6365 FT /vntifkey="29" FT /label=AG\promoter FT /note="Chicken ß-actin/rabbit ß-globin hybrid promoter" FT promoter 4641..5018 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only" FT intron 5393..6317 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter" FT CDS complement(3645..4505) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT CDS 59..1570 FT /vntifkey="4" FT /label=hPTBdelnls FT /note="Human polypyrimidine tract binding protein cDNA, delta nuclear localization seq." SQ Sequence 6373 BP; 1332 A; 1810 C; 1771 G; 1460 t; ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat 60 ggtcccctct agagtgatcc acatccggaa gctccccatc gacgtcacgg agggggaagt 120 catctccctg gggctgccct ttgggaaggt caccaacctc ctgatgctga aggggaaaaa 180 ccaggccttc atcgagatga acacggagga ggctgccaac accatggtga actactacac 240 ctcggtgacc cctgtgctgc gcggccagcc catctacatc cagttctcca accacaagga 300 gctgaagacc gacagctctc ccaaccaggc gcgggcccag gcggccctgc aggcggtgaa 360 ctcggtccag tcggggaacc tggccttggc tgcctcggcg gcggccgtgg acgcagggat 420 ggcgatggcc gggcagagcc ccgtgctcag gatcatcgtg gagaacctct tctaccctgt 480 gaccctggat gtgctgcacc agattttctc caagttcggc acagtgttga agatcatcac 540 cttcaccaag aacaaccagt tccaggccct gctgcagtat gcggaccccg tgagcgccca 600 gcacgccaag ctgtcgctgg acgggcagaa catctacaac gcctgctgca cgctgcgcat 660 cgacttttcc aagctcacca gcctcaacgt caagtacaac aatgacaaga gccgtgacta 720 cacacgccca gacctgcctt ccggggacag ccagccctcg ctggaccaga ccatggccgc 780 ggccttcggt gcacctggta taatctcagc ctctccgtat gcaggagctg gtttccctcc 840 cacctttgcc attcctcaag ctgcaggcct ttccgttccg aacgtccacg gcgccctggc 900 ccccctggcc atcccctcgg cggcggcggc agctgcggcg gcaggtcgga tcgccatccc 960 gggcctggcg ggggcaggaa attctgtatt gctggtcagc aacctcaacc cagagagagt 1020 cacaccccaa agcctcttta ttcttttcgg cgtctacggt gacgtgcagc gcgtgaagat 1080 cctgttcaat aagaaggaga acgccctagt gcagatggcg gacggcaacc aggcccagct 1140 ggccatgagc cacctgaacg ggcacaagct gcacgggaag cccatccgca tcacgctctc 1200 gaagcaccag aacgtgcagc tgccccgcga gggccaggag gaccagggcc tgaccaagga 1260 ctacggcaac tcacccctgc accgcttcaa gaagccgggc tccaagaact tccagaacat 1320 attcccgccc tcggccacgc tgcacctctc caacatcccg ccctcagtct ccgaggagga 1380 tctcaaggtc ctgttttcca gcaatggggg cgtcgtcaaa ggattcaagt tcttccagaa 1440 ggaccgcaag atggcactga tccagatggg ctccgtggag gaggcggtcc aggccctcat 1500 tgacctgcac aaccacgacc tcggggagaa ccaccacctg cgggtctcct tctccaagtc 1560 caccatctag ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc 1620 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa 1680 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 1740 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 1800 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca 1860 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc 1920 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 1980 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 2040 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 2100 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 2160 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 2220 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 2280 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc 2340 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 2400 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 2460 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt 2520 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 2580 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 2640 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 2700 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2760 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga 2820 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 2880 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 2940 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 3000 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 3060 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 3120 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 3180 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 3240 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 3300 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 3360 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 3420 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 3480 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 3540 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 3600 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 3660 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 3720 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 3780 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 3840 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 3900 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 3960 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 4020 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 4080 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 4140 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 4200 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 4260 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 4320 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 4380 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 4440 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 4500 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 4560 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 4620 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac 4680 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 4740 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 4800 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 4860 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 4920 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 4980 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 5040 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 5100 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc 5160 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag 5220 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa 5280 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc 5340 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 5400 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 5460 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 5520 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 5580 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 5640 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 5700 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 5760 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 5820 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 5880 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg 5940 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat 6000 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat 6060 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca 6120 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc 6180 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg 6240 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct 6300 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg 6360 gcaaagaatt gaa 6373 //