LOCUS       pCAGGS-E-hPTB           6535 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5260.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5260
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|437049061|
COMMENT     VNTNAME|pCAGGS-E-hPTB|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 0 0 0 1 6535 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1740 0 6534 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 7 0 385 0 934 0 2563 0 
COMMENT            5179 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 52 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1734 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "E-tag" 22 0 0 11 49 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "hPTB" 4 0 0 59 1732 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 1 2346 2428 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 2865 3266 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "ampicillin" 4 0 1 3807 4667 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4803 5180 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "AG promoter" 29 0 0 5186 6527 1 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "intron AG promoter" 15 0 0 5555 6479 1 
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COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 2570 2645 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1820 1911 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-hPTB'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 02/02/07 10:31" 1) 
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FEATURES             Location/Qualifiers
     misc_marker     11..49
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     rep_origin      2570..2645
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        complement(2346..2428)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="Escherichia coli lac operon promoter"
     terminator      1820..1911
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     rep_origin      complement(2865..3266)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      2676..2750
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2676..2750)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     misc_feature    1912..2268
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        5186..6527
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="Chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4803..5180
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     intron          5555..6479
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
     CDS             complement(3807..4667)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     CDS             59..1732
                     /vntifkey="4"
                     /label=hPTB
                     /note="Human polypyrimidine tract binding protein cDNA"
BASE COUNT     1377 a      1848 c      1815 g      1495 t 
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat 
       61 ggacggcatt gtcccagata tagccgttgg tacaaagcgg ggatctgacg agcttttctc 
      121 tacttgtgtc actaacggac cgtttatcat gagcagcaac tcggcttctg cagcaaacgg 
      181 aaatgacagc aagaagttca aaggtgacag ccgaagtgca ggcgtcccct ctagagtgat 
      241 ccacatccgg aagctcccca tcgacgtcac ggagggggaa gtcatctccc tggggctgcc 
      301 ctttgggaag gtcaccaacc tcctgatgct gaaggggaaa aaccaggcct tcatcgagat 
      361 gaacacggag gaggctgcca acaccatggt gaactactac acctcggtga cccctgtgct 
      421 gcgcggccag cccatctaca tccagttctc caaccacaag gagctgaaga ccgacagctc 
      481 tcccaaccag gcgcgggccc aggcggccct gcaggcggtg aactcggtcc agtcggggaa 
      541 cctggccttg gctgcctcgg cggcggccgt ggacgcaggg atggcgatgg ccgggcagag 
      601 ccccgtgctc aggatcatcg tggagaacct cttctaccct gtgaccctgg atgtgctgca 
      661 ccagattttc tccaagttcg gcacagtgtt gaagatcatc accttcacca agaacaacca 
      721 gttccaggcc ctgctgcagt atgcggaccc cgtgagcgcc cagcacgcca agctgtcgct 
      781 ggacgggcag aacatctaca acgcctgctg cacgctgcgc atcgactttt ccaagctcac 
      841 cagcctcaac gtcaagtaca acaatgacaa gagccgtgac tacacacgcc cagacctgcc 
      901 ttccggggac agccagccct cgctggacca gaccatggcc gcggccttcg gtgcacctgg 
      961 tataatctca gcctctccgt atgcaggagc tggtttccct cccacctttg ccattcctca 
     1021 agctgcaggc ctttccgttc cgaacgtcca cggcgccctg gcccccctgg ccatcccctc 
     1081 ggcggcggcg gcagctgcgg cggcaggtcg gatcgccatc ccgggcctgg cgggggcagg 
     1141 aaattctgta ttgctggtca gcaacctcaa cccagagaga gtcacacccc aaagcctctt 
     1201 tattcttttc ggcgtctacg gtgacgtgca gcgcgtgaag atcctgttca ataagaagga 
     1261 gaacgcccta gtgcagatgg cggacggcaa ccaggcccag ctggccatga gccacctgaa 
     1321 cgggcacaag ctgcacggga agcccatccg catcacgctc tcgaagcacc agaacgtgca 
     1381 gctgccccgc gagggccagg aggaccaggg cctgaccaag gactacggca actcacccct 
     1441 gcaccgcttc aagaagccgg gctccaagaa cttccagaac atattcccgc cctcggccac 
     1501 gctgcacctc tccaacatcc cgccctcagt ctccgaggag gatctcaagg tcctgttttc 
     1561 cagcaatggg ggcgtcgtca aaggattcaa gttcttccag aaggaccgca agatggcact 
     1621 gatccagatg ggctccgtgg aggaggcggt ccaggccctc attgacctgc acaaccacga 
     1681 cctcggggag aaccaccacc tgcgggtctc cttctccaag tccaccatct agctcgagga 
     1741 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct 
     1801 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa 
     1861 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg 
     1921 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca 
     1981 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa 
     2041 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt 
     2101 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt 
     2161 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct 
     2221 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca 
     2281 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 
     2341 caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt 
     2401 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     2461 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 
     2521 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 
     2581 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 
     2641 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg 
     2701 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     2761 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta 
     2821 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 
     2881 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     2941 ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa 
     3001 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     3061 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     3121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     3181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     3241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     3301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     3361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     3421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
     3481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     3541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
     3601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
     3661 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
     3721 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
     3781 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
     3841 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
     3901 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
     3961 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
     4021 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
     4081 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 
     4141 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
     4201 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
     4261 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
     4321 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
     4381 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 
     4441 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
     4501 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
     4561 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
     4621 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
     4681 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
     4741 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg 
     4801 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 
     4861 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 
     4921 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 
     4981 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 
     5041 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 
     5101 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 
     5161 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat 
     5221 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc 
     5281 gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg 
     5341 gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 
     5401 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 
     5461 gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc 
     5521 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc 
     5581 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga 
     5641 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg 
     5701 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg 
     5761 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg 
     5821 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt 
     5881 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca 
     5941 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg 
     6001 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 
     6061 ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg 
     6121 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg 
     6181 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca 
     6241 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg 
     6301 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc 
     6361 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg 
     6421 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc 
     6481 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgaa 
//
