ID pCAGGS-E-hPTB standard; circular DNA; ; 6535 BP. DE LMBP 5260. CC Accession number: LMBP 5260 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 24-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 11..49 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT rep_origin 2570..2645 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter complement(2346..2428) FT /vntifkey="30" FT /label=lac\promoter FT /note="Escherichia coli lac operon promoter" FT terminator 1820..1911 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT rep_origin complement(2865..3266) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT terminator 2676..2750 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2676..2750) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT misc_feature 1912..2268 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter 5186..6527 FT /vntifkey="29" FT /label=AG\promoter FT /note="Chicken ß-actin/rabbit ß-globin hybrid promoter" FT promoter 4803..5180 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only" FT intron 5555..6479 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter" FT CDS complement(3807..4667) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT CDS 59..1732 FT /vntifkey="4" FT /label=hPTB FT /note="Human polypyrimidine tract binding protein cDNA" SQ Sequence 6535 BP; 1377 A; 1848 C; 1815 G; 1495 t; ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat 60 ggacggcatt gtcccagata tagccgttgg tacaaagcgg ggatctgacg agcttttctc 120 tacttgtgtc actaacggac cgtttatcat gagcagcaac tcggcttctg cagcaaacgg 180 aaatgacagc aagaagttca aaggtgacag ccgaagtgca ggcgtcccct ctagagtgat 240 ccacatccgg aagctcccca tcgacgtcac ggagggggaa gtcatctccc tggggctgcc 300 ctttgggaag gtcaccaacc tcctgatgct gaaggggaaa aaccaggcct tcatcgagat 360 gaacacggag gaggctgcca acaccatggt gaactactac acctcggtga cccctgtgct 420 gcgcggccag cccatctaca tccagttctc caaccacaag gagctgaaga ccgacagctc 480 tcccaaccag gcgcgggccc aggcggccct gcaggcggtg aactcggtcc agtcggggaa 540 cctggccttg gctgcctcgg cggcggccgt ggacgcaggg atggcgatgg ccgggcagag 600 ccccgtgctc aggatcatcg tggagaacct cttctaccct gtgaccctgg atgtgctgca 660 ccagattttc tccaagttcg gcacagtgtt gaagatcatc accttcacca agaacaacca 720 gttccaggcc ctgctgcagt atgcggaccc cgtgagcgcc cagcacgcca agctgtcgct 780 ggacgggcag aacatctaca acgcctgctg cacgctgcgc atcgactttt ccaagctcac 840 cagcctcaac gtcaagtaca acaatgacaa gagccgtgac tacacacgcc cagacctgcc 900 ttccggggac agccagccct cgctggacca gaccatggcc gcggccttcg gtgcacctgg 960 tataatctca gcctctccgt atgcaggagc tggtttccct cccacctttg ccattcctca 1020 agctgcaggc ctttccgttc cgaacgtcca cggcgccctg gcccccctgg ccatcccctc 1080 ggcggcggcg gcagctgcgg cggcaggtcg gatcgccatc ccgggcctgg cgggggcagg 1140 aaattctgta ttgctggtca gcaacctcaa cccagagaga gtcacacccc aaagcctctt 1200 tattcttttc ggcgtctacg gtgacgtgca gcgcgtgaag atcctgttca ataagaagga 1260 gaacgcccta gtgcagatgg cggacggcaa ccaggcccag ctggccatga gccacctgaa 1320 cgggcacaag ctgcacggga agcccatccg catcacgctc tcgaagcacc agaacgtgca 1380 gctgccccgc gagggccagg aggaccaggg cctgaccaag gactacggca actcacccct 1440 gcaccgcttc aagaagccgg gctccaagaa cttccagaac atattcccgc cctcggccac 1500 gctgcacctc tccaacatcc cgccctcagt ctccgaggag gatctcaagg tcctgttttc 1560 cagcaatggg ggcgtcgtca aaggattcaa gttcttccag aaggaccgca agatggcact 1620 gatccagatg ggctccgtgg aggaggcggt ccaggccctc attgacctgc acaaccacga 1680 cctcggggag aaccaccacc tgcgggtctc cttctccaag tccaccatct agctcgagga 1740 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct 1800 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa 1860 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg 1920 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca 1980 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa 2040 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt 2100 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt 2160 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct 2220 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca 2280 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 2340 caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt 2400 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 2460 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 2520 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 2580 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 2640 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg 2700 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 2760 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta 2820 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 2880 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 2940 ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa 3000 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 3060 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 3120 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 3180 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 3240 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 3300 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 3360 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 3420 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 3480 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 3540 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 3600 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 3660 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 3720 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 3780 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 3840 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 3900 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 3960 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 4020 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 4080 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 4140 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 4200 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 4260 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 4320 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 4380 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 4440 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 4500 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 4560 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 4620 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 4680 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 4740 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg 4800 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 4860 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 4920 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 4980 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 5040 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 5100 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 5160 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat 5220 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc 5280 gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg 5340 gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 5400 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 5460 gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc 5520 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc 5580 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga 5640 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg 5700 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg 5760 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg 5820 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt 5880 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca 5940 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg 6000 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 6060 ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg 6120 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg 6180 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca 6240 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg 6300 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc 6360 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg 6420 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc 6480 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgaa 6535 //