LOCUS       pCAGGS-FLAG-hHNRPK        6161 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5269.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5269
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|456489557|
COMMENT     VNTNAME|pCAGGS-FLAG-hHNRPK|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-FLAG-hHNRPK" 0 6161 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "EcoRI" "KpnI" "SalI" "DraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6161 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 6160 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 42 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 1439 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "DraI" "tttaaa" 4 0 5 142 0 1602 0 3374 0 3393 
COMMENT            0 4085 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "intron AG promoter" 15 0 0 5186 6110 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4913..5837") 
COMMENT        #5=(CFSignal (CObList) "AG promoter" 29 0 0 4817 6158 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4544..5885") 
COMMENT        #6=(CFSignal (CObList) "ampicillin" 4 0 1 3434 4294 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3161..4021)") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 2492 2893 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2219..2620)") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 1973 2055 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1700..1782)") 
COMMENT        #9=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4434 4811 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4161..4538") 
COMMENT        #10=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1539 1895 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "10..36") 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 2197 2272 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2197..2272") 
COMMENT        #13=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1447 1538 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1447..1538") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2303 2377 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2302..2377") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2303 2377 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2302..2377)") 
COMMENT        #16=(CFSignal (CObList) "hHNRPK" 4 0 0 43 1437 0 (CStringList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "Last modified: 24-OCT-2007" 2 0 0 0 0 0 
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COMMENT                    "pCAGGS-FLAG-hHNRPK" 2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pCAGGS-FLAG-hHNRPK'" 1) 
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COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: margotbaetens" 
COMMENT                          1) 
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COMMENT                          "Created: 09/03/07 10:39" 1) 
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COMMENT                          "Last Modified: 10/24/07 12:28" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6161 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 13-AUG-2007" 1))) 
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COMMENT                          "EMAIL: <a href=\"mailto:margot.baetens@dmbr.ugent.be\">margot.baetens@dmbr.ugent.be</a>" 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
     intron          5186..6110
                     /vntifkey="15"
                     /label=intron\AG\promoter
     promoter        4817..6158
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     CDS             complement(3434..4294)
                     /vntifkey="4"
                     /label=ampicillin
     rep_origin      complement(2492..2893)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2303..2377)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      2303..2377
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      2197..2272
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      1447..1538
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        4434..4811
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        complement(1973..2055)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             43..1437
                     /vntifkey="4"
                     /label=hHNRPK
     misc_feature    1539..1895
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of ß-globin gene"
BASE COUNT     1403 a      1561 c      1684 g      1513 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggaaac tgaacagcca 
       61 gaagaaacct tccctaacac tgaaaccaat ggtgaatttg gtaaacgccc tgcagaagat 
      121 atggaagagg aacaagcatt taaaagatct agaaacactg atgagatggt tgaattacgc 
      181 attctgcttc agagcaagaa tgctggggca gtgattggaa aaggaggcaa gaatattaag 
      241 gctctccgta cagactacaa tgccagtgtt tcagtcccag acagcagtgg ccccgagcgc 
      301 atattgagta tcagtgctga tattgaaaca attggagaaa ttctgaagaa aatcatccct 
      361 accttggaag agggcctgca gttgccatca cccactgcaa ccagccagct cccgctcgaa 
      421 tctgatgctg tggaatgctt aaattaccaa cactataaag gaagtgactt tgactgcgag 
      481 ttgaggctgt tgattcatca gagtctagca ggaggaatta ttggggtcaa aggtgctaaa 
      541 attaaagaac ttcgagagaa cactcaaacc accatcaagc ttttccagga atgctgtcct 
      601 cattccactg acagagttgt tcttattgga ggaaaacccg atagggttgt agagtgcata 
      661 aagatcatcc ttgatcttat atctgagtct cccatcaaag gacgtgcaca gccttatgat 
      721 cccaattttt acgatgaaac ctatgattat ggtggtttta caatgatgtt tgatgaccgt 
      781 cgcggacgcc cagtgggatt tcccatgcgg ggaagaggtg gttttgacag aatgcctcct 
      841 ggtcggggtg ggcgtcccat gcctccatct agaagagatt atgatgatat gagccctcgt 
      901 cgaggaccac ctccccctcc tcccggacga ggcggccggg gtggtagcag agctcggaat 
      961 cttcctcttc ctccaccacc accacctaga gggggagacc tcatggccta tgacagaaga 
     1021 gggagacctg gagaccgtta cgacggcatg gttggtttca gtgctgatga aacttgggac 
     1081 tctgcaatag atacatggag cccatcagaa tggcagatgg cttatgaacc acagggtggc 
     1141 tccggatatg attattccta tgcagggggt cgtggctcat atggtgatct tggtggacct 
     1201 attattacta cacaagtaac tattcccaaa gatttggctg gatctattat tggcaaaggt 
     1261 ggtcagcgga ttaaacaaat ccgtcatgag tcgggagctt cgatcaaaat tgatgagcct 
     1321 ttagaaggat ccgaagatcg gatcattacc attacaggaa cacaggacca gatacagaat 
     1381 gcacagtatt tgctgcagaa cagtgtgaag cagtatgcag atgttgaagg attctaagtc 
     1441 gacgaagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat 
     1501 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg 
     1561 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat 
     1621 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta 
     1681 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa 
     1741 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca 
     1801 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc 
     1861 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc 
     1921 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 
     1981 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca 
     2041 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc 
     2101 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 
     2161 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     2221 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     2281 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag 
     2341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     2401 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa 
     2461 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     2521 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     2581 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 
     2641 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 
     2701 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 
     2761 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 
     2821 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 
     2881 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 
     2941 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 
     3001 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 
     3061 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 
     3121 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 
     3181 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 
     3241 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 
     3301 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 
     3361 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 
     3421 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 
     3481 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 
     3541 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 
     3601 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 
     3661 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 
     3721 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 
     3781 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 
     3841 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 
     3901 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 
     3961 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 
     4021 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 
     4081 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 
     4141 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 
     4201 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 
     4261 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 
     4321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 
     4381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg atcgacattg 
     4441 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 
     4501 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 
     4561 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 
     4621 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 
     4681 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 
     4741 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 
     4801 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 
     4861 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 
     4921 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 
     4981 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 
     5041 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt 
     5101 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 
     5161 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 
     5221 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 
     5281 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt 
     5341 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg 
     5401 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc 
     5461 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg 
     5521 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc 
     5581 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg 
     5641 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 
     5701 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc 
     5761 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 
     5821 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta 
     5881 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 
     5941 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 
     6001 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 
     6061 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 
     6121 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a 
//
