ID pCAGGS-FLAG-hHNRPK standard; circular DNA; ; 6161 BP. DE LMBP 5269. CC Accession number: LMBP 5269 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 24-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT intron 5186..6110 FT /vntifkey="15" FT /label=intron\AG\promoter FT promoter 4817..6158 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT CDS complement(3434..4294) FT /vntifkey="4" FT /label=ampicillin FT rep_origin complement(2492..2893) FT /vntifkey="33" FT /label=pMB1\ori FT terminator complement(2303..2377) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator 2303..2377 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT rep_origin 2197..2272 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 1447..1538 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT promoter 4434..4811 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter complement(1973..2055) FT /vntifkey="30" FT /label=lac\promoter FT CDS 43..1437 FT /vntifkey="4" FT /label=hHNRPK FT misc_feature 1539..1895 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of ß-globin gene" SQ Sequence 6161 BP; 1403 A; 1561 C; 1684 G; 1513 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggaaac tgaacagcca 60 gaagaaacct tccctaacac tgaaaccaat ggtgaatttg gtaaacgccc tgcagaagat 120 atggaagagg aacaagcatt taaaagatct agaaacactg atgagatggt tgaattacgc 180 attctgcttc agagcaagaa tgctggggca gtgattggaa aaggaggcaa gaatattaag 240 gctctccgta cagactacaa tgccagtgtt tcagtcccag acagcagtgg ccccgagcgc 300 atattgagta tcagtgctga tattgaaaca attggagaaa ttctgaagaa aatcatccct 360 accttggaag agggcctgca gttgccatca cccactgcaa ccagccagct cccgctcgaa 420 tctgatgctg tggaatgctt aaattaccaa cactataaag gaagtgactt tgactgcgag 480 ttgaggctgt tgattcatca gagtctagca ggaggaatta ttggggtcaa aggtgctaaa 540 attaaagaac ttcgagagaa cactcaaacc accatcaagc ttttccagga atgctgtcct 600 cattccactg acagagttgt tcttattgga ggaaaacccg atagggttgt agagtgcata 660 aagatcatcc ttgatcttat atctgagtct cccatcaaag gacgtgcaca gccttatgat 720 cccaattttt acgatgaaac ctatgattat ggtggtttta caatgatgtt tgatgaccgt 780 cgcggacgcc cagtgggatt tcccatgcgg ggaagaggtg gttttgacag aatgcctcct 840 ggtcggggtg ggcgtcccat gcctccatct agaagagatt atgatgatat gagccctcgt 900 cgaggaccac ctccccctcc tcccggacga ggcggccggg gtggtagcag agctcggaat 960 cttcctcttc ctccaccacc accacctaga gggggagacc tcatggccta tgacagaaga 1020 gggagacctg gagaccgtta cgacggcatg gttggtttca gtgctgatga aacttgggac 1080 tctgcaatag atacatggag cccatcagaa tggcagatgg cttatgaacc acagggtggc 1140 tccggatatg attattccta tgcagggggt cgtggctcat atggtgatct tggtggacct 1200 attattacta cacaagtaac tattcccaaa gatttggctg gatctattat tggcaaaggt 1260 ggtcagcgga ttaaacaaat ccgtcatgag tcgggagctt cgatcaaaat tgatgagcct 1320 ttagaaggat ccgaagatcg gatcattacc attacaggaa cacaggacca gatacagaat 1380 gcacagtatt tgctgcagaa cagtgtgaag cagtatgcag atgttgaagg attctaagtc 1440 gacgaagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat 1500 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg 1560 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat 1620 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta 1680 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa 1740 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca 1800 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc 1860 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc 1920 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 1980 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca 2040 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc 2100 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 2160 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 2220 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 2280 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag 2340 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 2400 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa 2460 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 2520 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 2580 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 2640 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 2700 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 2760 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 2820 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 2880 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 2940 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 3000 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 3060 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 3120 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 3180 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 3240 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 3300 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 3360 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 3420 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 3480 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 3540 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 3600 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 3660 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 3720 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 3780 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 3840 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 3900 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 3960 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 4020 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 4080 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 4140 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 4200 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 4260 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 4320 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 4380 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg atcgacattg 4440 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 4500 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 4560 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 4620 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 4680 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 4740 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 4800 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 4860 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 4920 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 4980 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 5040 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt 5100 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 5160 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 5220 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 5280 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt 5340 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg 5400 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc 5460 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg 5520 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc 5580 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg 5640 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 5700 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc 5760 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 5820 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta 5880 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 5940 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 6000 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 6060 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 6120 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a 6161 //