ID pCAGGS-FLAG-hHuR standard; circular DNA; ; 5747 BP. DE LMBP 5270. CC Accession number: LMBP 5270 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 24-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT intron 4772..5696 FT /vntifkey="15" FT /label=intron\AG\promoter FT promoter 4403..5744 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT CDS complement(3020..3880) FT /vntifkey="4" FT /label=ampicillin FT rep_origin complement(2078..2479) FT /vntifkey="33" FT /label=pMB1\ori FT terminator complement(1889..1963) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator 1889..1963 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT rep_origin 1783..1858 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 1033..1124 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT promoter 4020..4397 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter complement(1559..1641) FT /vntifkey="30" FT /label=lac\promoter FT CDS 43..1023 FT /vntifkey="4" FT /label=hHuR FT misc_feature 1125..1481 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of ß-globin gene" SQ Sequence 5747 BP; 1256 A; 1521 C; 1592 G; 1378 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatgtctaa tggttatgaa 60 gaccacatgg ccgaagactg caggggtgac atcgggagaa cgaatttgat cgtcaactac 120 ctccctcaga acatgaccca ggatgagtta cgaagcctgt tcagcagcat tggtgaagtt 180 gaatctgcaa aacttattcg ggataaagta gcaggacaca gcttgggcta tggctttgtg 240 aactacgtga ccgcgaagga tgcagagaga gcgatcaaca cgctgaacgg cttgaggctc 300 cagtcaaaaa ccattaaggt gtcgtatgct cgcccgagct cagaggtgat caaagacgcc 360 aacttgtaca tcagcgggct cccgcggacc atgacccaga aggacgtaga agacatgttc 420 tctcggtttg ggcggatcat caactcgcgg gtcctcgtgg atcagactac aggtttgtcc 480 agaggggttg cgtttatccg gtttgacaaa cggtcggagg cagaagaggc aattaccagt 540 ttcaatggtc ataaaccccc aggttcctct gagcccatca cagtgaagtt tgcagccaac 600 cccaaccaga acaaaaacgt ggcactcctc tcgcagctgt accactcgcc agcgcgacgg 660 ttcggaggcc ccgttcacca ccaggcgcag agattcaggt tctcccccat gggcgtcgat 720 cacatgagcg ggctctctgg cgtcaacgtg ccaggaaacg cctcctccgg ctggtgcatt 780 ttcatctaca acctggggca ggatgccgac gaggggatcc tctggcagat gtttgggccg 840 tttggtgccg tcaccaatgt gaaagtgatc cgcgacttca acaccaacaa gtgcaaaggg 900 tttggctttg tgaccatgac aaactatgaa gaagccgcga tggccatagc cagcctgaac 960 ggctaccgcc tgggggacaa aatcttacag gtttccttca aaaccaacaa gtcccacaaa 1020 taagtcgacg aagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 1080 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 1140 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 1200 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt 1260 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 1320 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 1380 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 1440 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag 1500 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 1560 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa 1620 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 1680 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 1740 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 1800 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 1860 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa 1920 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 1980 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc 2040 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 2100 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 2160 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc 2220 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2280 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2340 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2400 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 2460 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2520 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2580 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2640 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2700 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 2760 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2820 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 2880 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 2940 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 3000 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 3060 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3120 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3180 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 3240 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 3300 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 3360 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3420 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 3480 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 3540 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 3600 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 3660 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3720 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3780 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 3840 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 3900 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 3960 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgatcg 4020 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 4080 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 4140 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 4200 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 4260 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 4320 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 4380 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 4440 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 4500 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg 4560 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 4620 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 4680 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 4740 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 4800 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 4860 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt 4920 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg 4980 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg 5040 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg 5100 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc 5160 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg 5220 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg 5280 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg 5340 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc 5400 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc 5460 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg 5520 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc 5580 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 5640 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 5700 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa 5747 //