LOCUS       pCAGGS-FLAG-hnRNPC1        5678 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5271.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5271
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|454687021|
COMMENT     VNTNAME|pCAGGS-FLAG-hnRNPC1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-FLAG-hnRNPC1" 0 5678 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "KpnI" "BglII" "XhoI" "NcoI" "EcoRI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 4613 4612 1 5678 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 42 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglII" "agatct" 2 0 1 964 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 42 0 1707 0 4327 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 5677 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "intron AG promoter" 15 0 0 4703 5627 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4913..5837") 
COMMENT        #6=(CFSignal (CObList) "AG promoter" 29 0 0 4334 5675 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4544..5885") 
COMMENT        #7=(CFSignal (CObList) "ampicillin" 4 0 1 2951 3811 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3161..4021)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 2009 2410 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2219..2620)") 
COMMENT        #9=(CFSignal (CObList) "SV40 ori" 33 0 0 1714 1789 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1924..1999") 
COMMENT        #10=(CFSignal (CObList) "\337-globin polyA" 43 0 0 964 1055 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1174..1265") 
COMMENT        #11=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3951 4328 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4161..4538") 
COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 1490 1572 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1700..1782)") 
COMMENT        #13=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1056 1412 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1820 1894 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1819..1894") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1820 1894 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1819..1894)") 
COMMENT        #16=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "10..36") 
COMMENT        #17=(CFSignal (CObList) "hnRNPC1" 4 0 0 43 954 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "43..954")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "LMBP 5271" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 24-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    "pCAGGS-FLAG-hnRNPC1" 2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pCAGGS-FLAG-hnRNPC1'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5271." 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/14/07 01:57" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/24/07 12:32" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5678 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 14-AUG-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   0 0) 
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COMMENT                                 "" 1))) "LMBP 5271") 
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COMMENT                   "Annotations") 
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COMMENT                                   #45=(CLinePar 
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COMMENT                                        "   43..954" 1))) "hnRNPC1") 
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COMMENT                                        1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3161..4021)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4913..5837" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                   #61=(CLinePar 
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COMMENT                                        "FLAG epitope" 1) 
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COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4544..5885" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #73=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1490  End: 1572 (Complementary)" 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1700..1782)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
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COMMENT                                        "   1924..1999" 1))) "SV40 ori") 
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COMMENT                                   #85=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2219..2620)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
                     /note="FLAG epitope"
     intron          4703..5627
                     /vntifkey="15"
                     /label=intron\AG\promoter
     promoter        4334..5675
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     CDS             complement(2951..3811)
                     /vntifkey="4"
                     /label=ampicillin
     rep_origin      complement(2009..2410)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      complement(1820..1894)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1820..1894
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      1714..1789
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      964..1055
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        3951..4328
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        complement(1490..1572)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             43..954
                     /vntifkey="4"
                     /label=hnRNPC1
     misc_feature    1056..1412
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the ß-globin gene"
BASE COUNT     1278 a      1438 c      1589 g      1373 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggccag caacgttacc 
       61 aacaagacag atcctcgctc catgaactcc cgtgtattca ttgggaatct caacactctt 
      121 gtggtcaaga aatctgatgt ggaggcaatc ttttcgaagt atggcaaaat tgtgggctgc 
      181 tctgttcata agggctttgc cttcgttcag tatgttaatg agagaaatgc ccgggctgct 
      241 gtagcaggag aggatggcag aatgattgct ggccaggttt tagatattaa cctggctgca 
      301 gagccaaaag tgaaccgagg aaaagcaggt gtgaaacgat ctgcagcgga gatgtacggg 
      361 tcagtaacag aacacccttc tccgtcccct ctactcagct cctcttttga cttggactat 
      421 gactttcaac gggactatta tgataggatg tacagttacc cagcacgtgt acctcctcct 
      481 cctcctattg ctcgggctgt agtgccctcg aaacgtcagc gtgtatcagg aaacacttca 
      541 cgaaggggca aaagtggctt caattctaag agtggacagc ggggatcttc caagtctgga 
      601 aagttgaaag gagatgacct tcaggccatt aagaaggagc tgacccagat aaaacaaaaa 
      661 gtggattctc tcctggaaaa cctggaaaaa atggaaaagg aacagagcaa acaagcagta 
      721 gagatgaaga atgataagtc agaagaggag cagagcagca gctccgtgaa gaaagatgag 
      781 actaatgtga agatggagtc tgaggggggt gcagatgact ctgctgagga gggggaccta 
      841 ctggatgatg atgataatga agatcggggg gatgaccagc tggagttgat caaggatgat 
      901 gaaaaagagg ctgaggaagg agaggatgac agagacagcg ccaatggagg atgactcgac 
      961 gaagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg 
     1021 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt 
     1081 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg 
     1141 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa 
     1201 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc 
     1261 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc 
     1321 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca 
     1381 gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta 
     1441 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 
     1501 acgagccgga agcataaagt gtaaagcctg ggtgcctaat gagtgagcta actcacatta 
     1561 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc 
     1621 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     1681 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     1741 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     1801 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa 
     1861 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 
     1921 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc 
     1981 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
     2041 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     2101 tccacagaat cagggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     2161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     2221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     2281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     2341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     2401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     2461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     2521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     2581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     2641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     2701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     2761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     2821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     2881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     2941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     3001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     3061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     3121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     3181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     3241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     3301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     3361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     3421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     3481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     3541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     3601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     3661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     3721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     3781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     3841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     3901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgatc gacattgatt 
     3961 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 
     4021 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 
     4081 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 
     4141 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 
     4201 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 
     4261 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 
     4321 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc 
     4381 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg 
     4441 gggggggggg gggccccccc caggcggggc ggggcggggc gaggggcggg gcggggcgag 
     4501 gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc 
     4561 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc 
     4621 gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg 
     4681 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 
     4741 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg 
     4801 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg 
     4861 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg 
     4921 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc 
     4981 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg 
     5041 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg 
     5101 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct 
     5161 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg 
     5221 ggccggggag ggctcggggg aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag 
     5281 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc 
     5341 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg 
     5401 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg 
     5461 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg 
     5521 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct 
     5581 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg 
     5641 tgctggttat tgtgctgtct catcattttg gcaaagaa 
//
