ID pCD-Lck-Flag standard; circular DNA; ; 5496 BP. DE LMBP 5350. CC Accession number: LMBP 5350 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 17-APR-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(4500..5360) FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS 2199..2993 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT terminator 3169..3243 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(3169..3243) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(3557..3959) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(3382..3465) FT /vntifkey="30" FT /label=lac\promoter FT promoter 235..818 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT rep_origin 2032..2107 FT /vntifkey="33" FT /label=SV40\ori FT rep_origin 1361..1789 FT /vntifkey="33" FT /label=f1\ori FT promoter 863..879 FT /vntifkey="30" FT /label=T7\promoter FT misc_marker 968..991 FT /vntifkey="22" FT /label=FLAG\epitope FT terminator 1091..1315 FT /vntifkey="43" FT /label=BGH\polyA FT promoter 1794..2075 FT /vntifkey="29" FT /label=SV40\early\promoter FT CDS 914..952 FT /vntifkey="4" FT /label=mLck\myristoylation-targeting\sequence SQ Sequence 5496 BP; 1271 A; 1423 C; 1408 G; 1394 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 960 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 1020 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga 1080 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 1140 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 1200 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 1260 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 1320 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 1380 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 1440 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 1500 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 1560 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 1620 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 1680 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 1740 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 1800 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 1860 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 1920 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 1980 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 2040 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 2100 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 2160 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 2220 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 2280 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 2340 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 2400 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 2460 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 2520 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 2580 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 2640 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 2700 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 2760 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 2820 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 2880 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 2940 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 3000 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 3060 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 3120 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 3180 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 3240 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 3300 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 3360 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 3420 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 3480 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 3540 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 3600 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 3660 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 3720 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 3780 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 3840 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 3900 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 3960 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 4020 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4080 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4140 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 4200 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4260 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 4320 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 4380 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 4440 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 4500 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 4560 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 4620 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 4680 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 4740 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 4800 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 4860 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 4920 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 4980 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 5040 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 5100 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 5160 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 5220 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 5280 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 5340 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 5400 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 5460 ccgcgcacat ttccccgaaa agtgccacct gacgtc 5496 //