LOCUS       pcDNA3.1-Flag           5449 bp    DNA     circular     17-APR-2009
DEFINITION  LMBP 5351.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5351
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-APR-2009
            
COMMENT     VNTDATE|464360381|
COMMENT     VNTNAME|pcDNA3.1-Flag|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pcDNA3.1-Flag" 0 5449 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "Alw44I" "AvaI" "BamHI" "HincII" "HindIII" "NaeI" "NdeI" 
COMMENT         "PvuII" "SacI" "XmnI" "KpnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 1 5449 1 5449 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 33 0 3948 0 5194 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 2 1004 0 2094 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 956 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 3 1 0 235 0 3262 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 912 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NaeI" "gccggc" 4 0 3 1444 0 2785 0 3068 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 485 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 1293 0 2389 0 3458 0 0 
COMMENT            "") #8=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 819 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 1745 0 5126 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 951 0 0 "")) 
COMMENT       (CObList 
COMMENT        #11=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "863..879") 
COMMENT        #12=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 235 818 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "232..818") 
COMMENT        #13=(CFSignal (CObList) "lac promoter" 30 0 1 3335 3418 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4453 5313 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4519..5379)") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 3510 3912 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3576..3978)") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3122 3196 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3188..3262)") 
COMMENT        #17=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3122 3196 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3188..3262") 
COMMENT        #18=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 2154 2948 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2220..3014") 
COMMENT        #19=(CFSignal (CObList) "SV40 early promoter" 29 0 0 1749 2030 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1815..2096") 
COMMENT        #20=(CFSignal (CObList) "SV40 ori" 33 0 0 1987 2062 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2053..2128") 
COMMENT        #21=(CFSignal (CObList) "f1 ori" 33 0 0 1316 1744 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1382..1810") 
COMMENT        #22=(CFSignal (CObList) "BGH polyA" 43 0 0 1046 1270 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1112..1336") 
COMMENT        #23=(CFSignal (CObList) "FLAG epitope" 22 0 0 923 946 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #24=(CFSignal (CObList) "LMBP 5351" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #25=(CFSignal (CObList) "Last modified: 17-APR-2009" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #26=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #27=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pcDNA3.1-Flag" 2) 
COMMENT                   5 "" 0 4) 
COMMENT              #28=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pcDNA3.1-Flag'" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5351" 
COMMENT                          1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 11/30/07 12:59" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/17/09 09:44" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5449 bp" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #38=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #39=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #40=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #41=(CLinePar (CParagraph 0 (15 #24# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5351") 
COMMENT                     #42=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #43=(CLinePar (CParagraph 0 (15 #25# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 17-APR-2009"))) 
COMMENT                   "Annotations") 
COMMENT              #44=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2154 (3 #18# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2154  End: 2948" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2220..3014" 1))) 
COMMENT                                 "Tn5 neomycin resistance") 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4453 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4453  End: 5313 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4519..5379)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
COMMENT                     #54=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 923 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 923   End: 946 " 1))) 
COMMENT                                 "FLAG epitope"))) "Misc. Marker (1 total)") 
COMMENT                     #57=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 235 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 235   End: 818 " 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   232..818" 1))) 
COMMENT                                 "hCMV-IE promoter and enhancer") 
COMMENT                            #62=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1749 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1749  End: 2030" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1815..2096" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #66=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 863 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 863   End: 879 " 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   863..879" 1))) "T7 promoter") 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3335 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3335  End: 3418 (Complementary)" 
COMMENT                                        1))) "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1316 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1316  End: 1744" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1382..1810" 1))) "f1 ori") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1987 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1987  End: 2062" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2053..2128" 1))) "SV40 ori") 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3510 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3510  End: 3912 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3576..3978)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #86=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1046 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1046  End: 1270" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1112..1336" 1))) "BGH polyA") 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3122 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3122  End: 3196 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3188..3262)" 1))) 
COMMENT                                 "SV40 polyA late") 
COMMENT                            #95=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3122 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3122  End: 3196" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3188..3262" 1))) "SV40 polyA early"))) 
COMMENT                          "Terminator (3 total)"))) "Feature Map") 
COMMENT              #99=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #100=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #101=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #102=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 956 " 1))))) "BamHI: 1 site") 1 
COMMENT                           5 "GGATCC" "CCTAGG") 
COMMENT                     #103=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #104=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 912 " 1))))) "HindIII: 1 site") 
COMMENT                           1 5 "AAGCTT" "TTCGAA") 
COMMENT                     #106=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #107=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 485 " 1))))) "NdeI: 1 site") 2 
COMMENT                           4 "CATATG" "GTATAC") 
COMMENT                     #109=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #110=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 819 " 1))))) "SacI: 1 site") 5 
COMMENT                           1 "GAGCTC" "CTCGAG") 
COMMENT                     #112=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #113=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #10# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 951 " 1))))) "KpnI: 1 site") 5 
COMMENT                           1 "GGTACC" "CCATGG") 
COMMENT                     #115=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #116=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1004 " 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 2094 " 1))))) "AvaI: 2 sites") 
COMMENT                           1 5 "CYCGRG" "GRGCYC") 
COMMENT                     #119=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #120=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1745 " 1) 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 5126 " 1))))) "XmnI: 2 sites") 
COMMENT                           5 5 "GAANNNNTTC" "CTTNNNNAAG") 
COMMENT                     #123=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #124=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 33 " 1) 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3948 " 1) 
COMMENT                                     #127=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 5194 " 1))))) "Alw44I: 3 sites") 
COMMENT                           1 5 "GTGCAC" "CACGTG") 
COMMENT                     #128=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #129=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1 " 1) 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 235 " 1) 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 3262 " 1))))) "HincII: 3 sites") 
COMMENT                           3 3 "GTYRAC" "CARYTG") 
COMMENT                     #133=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #134=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1444 " 1) 
COMMENT                                     #136=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 2785 " 1) 
COMMENT                                     #137=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 3068 " 1))))) "NaeI: 3 sites") 
COMMENT                           3 3 "GCCGGC" "CGGCCG") 
COMMENT                     #138=(CRSFolderPar 
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FEATURES             Location/Qualifiers
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        complement(3335..3418)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             complement(4453..5313)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(3510..3912)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(3122..3196)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      3122..3196
                     /vntifkey="43"
                     /label=SV40\polyA\early
     CDS             2154..2948
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     promoter        1749..2030
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      1987..2062
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      1316..1744
                     /vntifkey="33"
                     /label=f1\ori
     terminator      1046..1270
                     /vntifkey="43"
                     /label=BGH\polyA
     misc_marker     923..946
                     /vntifkey="22"
                     /label=FLAG\epitope
BASE COUNT     1262 a      1411 c      1392 g      1384 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc 
      961 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc 
     1021 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 
     1081 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 
     1141 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 
     1201 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 
     1261 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 
     1321 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     1381 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     1441 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     1501 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 
     1561 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     1621 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 
     1681 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     1741 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 
     1801 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 
     1861 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 
     1921 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
     1981 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 
     2041 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 
     2101 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg 
     2161 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 
     2221 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 
     2281 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg 
     2341 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 
     2401 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 
     2461 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 
     2521 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 
     2581 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 
     2641 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg 
     2701 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 
     2761 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 
     2821 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 
     2881 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 
     2941 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 
     3001 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 
     3061 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 
     3121 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     3181 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     3241 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 
     3301 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 
     3361 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 
     3421 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 
     3481 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     3541 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     3601 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     3661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     3721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     3781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     3841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     3901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     3961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     4021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     4081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 
     4141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     4201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     4261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     4321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     4381 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     4441 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     4501 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     4561 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     4621 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     4681 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     4741 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     4801 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     4861 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     4921 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     4981 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     5041 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     5101 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     5161 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     5221 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     5281 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     5341 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     5401 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc 
//
