ID pcDNA3.1-Flag standard; circular DNA; ; 5449 BP. DE LMBP 5351. CC Accession number: LMBP 5351 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 17-APR-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 863..879 FT /vntifkey="30" FT /label=T7\promoter FT promoter 235..818 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT promoter complement(3335..3418) FT /vntifkey="30" FT /label=lac\promoter FT CDS complement(4453..5313) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(3510..3912) FT /vntifkey="33" FT /label=pMB1\ori FT terminator complement(3122..3196) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 3122..3196 FT /vntifkey="43" FT /label=SV40\polyA\early FT CDS 2154..2948 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT promoter 1749..2030 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 1987..2062 FT /vntifkey="33" FT /label=SV40\ori FT rep_origin 1316..1744 FT /vntifkey="33" FT /label=f1\ori FT terminator 1046..1270 FT /vntifkey="43" FT /label=BGH\polyA FT misc_marker 923..946 FT /vntifkey="22" FT /label=FLAG\epitope SQ Sequence 5449 BP; 1262 A; 1411 C; 1392 G; 1384 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc 960 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc 1020 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 1080 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 1140 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 1200 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 1260 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 1320 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 1380 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 1440 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 1500 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 1560 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 1620 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 1680 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 1740 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 1800 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 1860 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 1920 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 1980 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 2040 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 2100 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg 2160 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 2220 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 2280 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg 2340 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 2400 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 2460 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 2520 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 2580 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 2640 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg 2700 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 2760 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 2820 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 2880 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 2940 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 3000 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 3060 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 3120 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 3180 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 3240 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 3300 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 3360 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 3420 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 3480 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 3540 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 3600 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 3660 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 3720 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 3780 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 3840 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 3900 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 3960 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 4020 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 4080 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 4140 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 4200 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 4260 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 4320 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 4380 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 4440 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 4500 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 4560 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 4620 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 4680 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 4740 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 4800 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 4860 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 4920 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 4980 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 5040 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 5100 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 5160 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 5220 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 5280 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 5340 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 5400 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc 5449 //