ID pCAGGS-E-hA20-C103S standard; circular DNA; ; 7278 BP. DE LMBP 5355. CC Accession number: LMBP 5355 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 15-JUL-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT intron 6303..7227 FT /vntifkey="15" FT /label=intron\AG\promoter FT CDS complement(4555..5415) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(3613..4014) FT /vntifkey="33" FT /label=pMB1\ori FT terminator complement(3424..3498) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 3424..3498 FT /vntifkey="43" FT /label=SV40\polyA\early FT rep_origin 3318..3393 FT /vntifkey="33" FT /label=SV40\ori FT promoter complement(3094..3176) FT /vntifkey="30" FT /label=lac\promoter FT terminator 2568..2659 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT CDS 64..2436 FT /vntifkey="4" FT /label=hA20\mutant FT misc_marker 16..54 FT /vntifkey="22" FT /label=E-tag FT promoter 5934..7275 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT promoter 5551..5928 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT misc_feature 2660..3016 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT misc_feature 2437..2480 FT /vntifkey="21" FT /label=3'\UTR\of\hA20 FT mutation 370..372 FT /vntifkey="62" FT /label=C103S FT /note="WT: TGC" SQ Sequence 7278 BP; 1660 A; 1954 C; 1967 G; 1697 t; ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 60 gcaatggctg aacaagtcct tcctcaggct ttgtatttga gcaatatgcg gaaagctgtg 120 aagatacggg agagaactcc agaagacatt tttaaaccta ctaatgggat cattcatcat 180 tttaaaacca tgcaccgata cacactggaa atgttcagaa cttgccagtt ttgtcctcag 240 tttcgggaga tcatccacaa agccctcatc gacagaaaca tccaggccac cctggaaagc 300 cagaagaaac tcaactggtg tcgagaagtc cggaagcttg tggcgctgaa aacgaacggt 360 gacggcaata gcctcatgca tgccacttct cagtacatgt ggggcgttca ggacacagac 420 ttggtactga ggaaggcgct gttcagcacg ctcaaggaaa cagacacacg caactttaaa 480 ttccgctggc aactggagtc tctcaaatct caggaatttg ttgaaacggg gctttgctat 540 gatactcgga actggaatga tgaatgggac aatcttatca aaatggcttc cacagacaca 600 cccatggccc gaagtggact tcagtacaac tcactggaag aaatacacat atttgtcctt 660 tgcaacatcc tcagaaggcc aatcattgtc atttcagaca aaatgctaag aagtttggaa 720 tcaggttcca atttcgcccc tttgaaagtg ggtggaattt acttgcctct ccactggcct 780 gcccaggaat gctacagata ccccattgtt ctcggctatg acagccatca ttttgtaccc 840 ttggtgaccc tgaaggacag tgggcctgaa atccgagctg ttccacttgt taacagagac 900 cggggaagat ttgaagactt aaaagttcac tttttgacag atcctgaaaa tgagatgaag 960 gagaagctct taaaagagta cttaatggtg atagaaatcc ccgtccaagg ctgggaccat 1020 ggcacaactc atctcatcaa tgccgcaaag ttggatgaag ctaacttacc aaaagaaatc 1080 aatctggtag atgattactt tgaacttgtt cagcatgagt acaagaaatg gcaggaaaac 1140 agcgagcagg ggaggagaga ggggcacgcc cagaatccca tggaaccttc cgtgccccag 1200 ctttctctca tggatgtaaa atgtgaaacg cccaactgcc ccttcttcat gtctgtgaac 1260 acccagcctt tatgccatga gtgctcagag aggcggcaaa agaatcaaaa caaactccca 1320 aagctgaact ccaagccggg ccctgagggg ctccctggca tggcgctcgg ggcctctcgg 1380 ggagaagcct atgagccctt ggcgtggaac cctgaggagt ccactggggg gcctcattcg 1440 gccccaccga cagcacccag cccttttctg ttcagtgaga ccactgccat gaagtgcagg 1500 agccccggct gccccttcac actgaatgtg cagcacaacg gattttgtga acgttgccac 1560 aacgcccggc aacttcacgc cagccacgcc ccagaccaca caaggcactt ggatcccggg 1620 aagtgccaag cctgcctcca ggatgttacc aggacattta atgggatctg cagtacttgc 1680 ttcaaaagga ctacagcaga ggcctcctcc agcctcagca ccagcctccc tccttcctgt 1740 caccagcgtt ccaagtcaga tccctcgcgg ctcgtccgga gcccctcccc gcattcttgc 1800 cacagagctg gaaacgacgc ccctgctggc tgcctgtctc aagctgcacg gactcctggg 1860 gacaggacgg ggacgagcaa gtgcagaaaa gccggctgcg tgtattttgg gactccagaa 1920 aacaagggct tttgcacact gtgtttcatc gagtacagag aaaacaaaca ttttgctgct 1980 gcctcaggga aagtcagtcc cacagcgtcc aggttccaga acaccattcc gtgcctgggg 2040 agggaatgcg gcacccttgg aagcaccatg tttgaaggat actgccagaa gtgtttcatt 2100 gaagctcaga atcagagatt tcatgaggcc aaaaggacag aagagcaact gagatcgagc 2160 cagcgcagag atgtgcctcg aaccacacaa agcacctcaa ggcccaagtg cgcccgggcc 2220 tcctgcaaga acatcctggc ctgccgcagc gaggagctct gcatggagtg tcagcatccc 2280 aaccagagga tgggccctgg ggcccaccgg ggtgagcctg cccccgaaga cccccccaag 2340 cagcgttgcc gggcccccgc ctgtgatcat tttggcaatg ccaagtgcaa cggctactgc 2400 aacgaatgct ttcagttcaa gcagatgtat ggctaaccgg aaacaggtgg gtcacctcct 2460 gcaagaagtg gggcctcgag gtcgaggaat tcactcctca ggtgcaggct gcctatcaga 2520 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct 2580 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 2640 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 2700 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 2760 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 2820 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 2880 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 2940 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 3000 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 3060 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 3120 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 3180 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc 3240 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 3300 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 3360 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 3420 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3480 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3540 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3600 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3660 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga 3720 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3780 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3840 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3900 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3960 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 4020 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 4080 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 4140 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 4200 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 4260 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 4320 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 4380 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 4440 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 4500 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 4560 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 4620 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 4680 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 4740 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 4800 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 4860 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 4920 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 4980 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 5040 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 5100 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 5160 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 5220 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 5280 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 5340 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 5400 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 5460 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 5520 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt 5580 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 5640 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 5700 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 5760 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 5820 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 5880 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga 5940 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt 6000 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc 6060 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag 6120 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 6180 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc 6240 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac 6300 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac 6360 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt 6420 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg 6480 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc 6540 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg 6600 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg 6660 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg 6720 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt 6780 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 6840 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat 6900 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg 6960 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc 7020 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc 7080 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg 7140 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat 7200 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct 7260 catcattttg gcaaagaa 7278 //