LOCUS       pCAGGS-E-mABIN-1(54-388)        5814 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5360.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5360
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|457958511|
COMMENT     VNTNAME|pCAGGS-E-mABIN-1(54-388)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-mABIN-1(54-388)" 0 5814 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 1668219001 0 "" 0 "" "" "" "" "" "" "pCAGGSEhA20" 
COMMENT             "" 0 0 1 62 1 62 1 62 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 62 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 3743845 0 "" 0 "" "" "" "" "" "" 
COMMENT             "mABIN1(NM_021327)" "" 0 0 1 1006 63 1068 275 1280 0 "" "" "" "" "" 
COMMENT             "" (CObList) (CTerm 2 0 275 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 1280 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 1 1 0 1752432761 0 "" 0 "TGACCC" "" "" "" "" "" "" "" 0 0 
COMMENT             1 6 1069 1074 1 6 0 "tgaccc" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #3=(CFragment 3 1 0 1752432761 0 "" 0 "" "" "" "" "" "" "pCAGGS" "" 0 0 
COMMENT             1 2460 1075 3534 62 2521 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 62 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 2521 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #4=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pCAGGSEhA20" "" 0 0 1 
COMMENT             2280 3535 5814 4999 7278 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 4999 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
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COMMENT        (CStringList "HindIII" "EcoRI" "BglI" "NotI" "BglII" "PvuI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 0 0 0 1 5814 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1570 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4 0 340 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 6 611 0 1893 0 3285 0 
COMMENT            4193 0 4315 0 4386 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1104 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3535 0 0 "")) 
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COMMENT             (CStringList) (CStringList) 1 1 1 1 "1196..1552") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1960 2034 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1960..2034)") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1960 2034 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1960..2034") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 2149 2550 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2149..2550)") 
COMMENT        #16=(CFSignal (CObList) "lac promoter" 30 0 1 1630 1712 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1630..1712)") 
COMMENT        #17=(CFSignal (CObList) "SV40 ori" 33 0 0 1854 1929 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1854..1929") 
COMMENT        #18=(CFSignal (CObList) "intron AG promoter" 15 0 0 4839 5763 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4839..5763") 
COMMENT        #19=(CFSignal (CObList) "AG promoter" 29 0 0 4470 5811 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4470..5811") 
COMMENT        #20=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4087 4464 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4087..4464") 
COMMENT        #21=(CFSignal (CObList) "ampicillin" 4 0 1 3091 3951 0 (CStringList) 
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COMMENT        #22=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1104 1195 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1104..1195") 
COMMENT        #23=(CFSignal (CObList) "mABIN-1(54-388)" 4 0 0 64 1071 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-mABIN-1(54-388)'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: margotbaetens" 
COMMENT                          1) 
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COMMENT                          "Created: 09/20/07 10:41" 1) 
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COMMENT                          "Last Modified: 10/24/07 12:09" 1) 
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COMMENT                          "length: 5814 bp" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 20-SEP-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "EMAIL: <a href=\"mailto:margot.baetens@dmbr.ugent.be\">margot.baetens@dmbr.ugent.be</a>" 
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COMMENT                          "#3: SEQUENCE TGACCC") 
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COMMENT                          "#5: FRAGMENT of <a href=\"vnti:dnarna/pCAGGSEhA20?sel=4999-7278\">pCAGGSEhA20</a> "))) 
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COMMENT                                        "Mouse A20-binding inhibitor of NF-kappaB activation 1 cDNA; aa 54-388" 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     misc_feature    1196..1552
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit  ß-globin gene"
     terminator      complement(1960..2034)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1960..2034
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(2149..2550)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      1104..1195
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        complement(1630..1712)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="Escherichia coli lac operon promoter"
     rep_origin      1854..1929
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     intron          4839..5763
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4470..5811
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4087..4464
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     CDS             complement(3091..3951)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance; coding sequence"
     CDS             64..1071
                     /vntifkey="4"
                     /label=mABIN-1(54-388)
                     /note="Mouse A20-binding inhibitor of NF-kappaB activation 1 cDNA; aa 54-388"
BASE COUNT     1268 a      1575 c      1637 g      1334 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcgatggaag cgtccagact ccggcagaag gcagaggagc tggtcaagga cagcgagctg 
      121 tcaccaccga catctgcccc ctccttggtc tcctttgatg acctggctga gctcacagga 
      181 caggatacaa aggtccaggt acatcctgct accagcactg ccgccaccac caccgccacc 
      241 gccaccacgg gaaactccat ggagaagccc gagccagcct ccaaatctcc gtccaatggc 
      301 gcctcctcgg actttgaagt ggtccctact gaggagcaga attcacccga aactggcagc 
      361 caccctacga acatgatgga cctggggccc ccacccccag aggacagcaa cctgaagctc 
      421 cacctgcagc gcctggagac cacccttagc gtgtgtgcag aggagccaga ccacagccag 
      481 ctcttcaccc acctgggccg catggccctc gagttcaaca ggttggcctc caaagtgcat 
      541 aaaaatgagc agcgcacctc catcctgcag accttatgtg agcagctgcg ccaggagaat 
      601 gaagccctga aggccaagct ggacaagggc ctggaacagc gggatctggc tgctgagagg 
      661 ctgcgggagg aaaacacgga gctcaagaaa ctgttgatga acagcagctg caaagaggga 
      721 ctctgtgggc agcccagctc cccaaagcca gagggtgctg gcaagaaggg cgtggctgga 
      781 cagcagcagg ccagtgtgat ggcgagtaaa gtccctgaag cgggggcctt tggagcagct 
      841 gagaagaaag tgaagttgct agaacagcaa cgcatggagc tgctggaagt gaacaagcag 
      901 tgggaccagc atttccggtc catgaagcag cagtatgagc agaagatcac agagcttcgc 
      961 cagaagctgg tggacctgca gaaacaggta actgagctgg aggccgaacg ggagcagaag 
     1021 cagcgtgact ttgaccggaa actcctcctg gccaaatcga agatagagtg acccccaatg 
     1081 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca 
     1141 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag 
     1201 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa 
     1261 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca 
     1321 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt 
     1381 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt 
     1441 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc 
     1501 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc 
     1561 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     1621 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg ggtgcctaat 
     1681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 
     1741 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 
     1801 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     1861 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 
     1921 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat 
     1981 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 
     2041 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc 
     2101 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 
     2161 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     2221 caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc 
     2281 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     2341 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     2401 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     2461 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     2521 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     2581 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     2641 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     2701 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     2761 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     2821 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     2881 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     2941 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     3001 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     3061 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     3121 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     3181 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     3241 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     3301 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     3361 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     3421 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     3481 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     3541 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
     3601 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
     3661 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
     3721 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
     3781 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     3841 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     3901 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     3961 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     4021 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
     4081 tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 
     4141 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 
     4201 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 
     4261 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 
     4321 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 
     4381 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 
     4441 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc 
     4501 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg 
     4561 cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg cggggcgagg 
     4621 ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 
     4681 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 
     4741 gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc gcctcgcgcc 
     4801 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt 
     4861 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc 
     4921 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg 
     4981 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg 
     5041 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg 
     5101 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg 
     5161 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc 
     5221 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg 
     5281 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg 
     5341 gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg cccccggagc 
     5401 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag 
     5461 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc 
     5521 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc 
     5581 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc 
     5641 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg 
     5701 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta 
     5761 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaa 
//
