LOCUS       pCAGGS-E-mABIN-1(420-647)        5665 bp    DNA     circular     29-MAY-2007
DEFINITION  LMBP 5361.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5361
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
            
COMMENT     VNTDATE|447683958|
COMMENT     VNTNAME|pCAGGS-E-mABIN-1(420-647)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        (CStringList "HindIII" "NotI" "PvuI" "Eco147I" "AlwNI" "EcoRI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 13 90 1 5665 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1421 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3386 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "Eco147I" "aggcct" 4 0 3 137 0 703 0 1790 0 0 
COMMENT            "") 
COMMENT        #4=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 3 719 0 2539 0 5618 0 0 
COMMENT            "") #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "")) 
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COMMENT        #8=(CFSignal (CObList) "\337-globin polyA" 43 0 0 955 1046 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "955..1046") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 1481 1563 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "intron AG promoter" 15 0 0 4690 5614 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4690..5614") 
COMMENT        #11=(CFSignal (CObList) "AG promoter" 29 0 0 4321 5662 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3938 4315 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3938..4315") 
COMMENT        #13=(CFSignal (CObList) "ampicillin" 4 0 1 2942 3802 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1047 1403 0 
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COMMENT        #15=(CFSignal (CObList) "SV40 ori" 33 0 0 1705 1780 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "mABIN-1(420-647)" 4 0 0 64 750 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: margotbaetens" 
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COMMENT                          "Created: 05/29/07 12:39" 1) 
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COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     misc_feature    1047..1403
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      complement(1811..1885)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1811..1885
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(2000..2401)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      955..1046
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="Rabbit ß-globin polyadenylation signal"
     promoter        complement(1481..1563)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="Escherichia coli lac operon promoter"
     rep_origin      1705..1780
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     intron          4690..5614
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4321..5662
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="Chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3938..4315
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     CDS             complement(2942..3802)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     CDS             64..750
                     /vntifkey="4"
                     /label=mABIN-1(420-647)
                     /note="Mouse A20-binding inhibitor of NF-kappa B activation cDNA; aa 420-647"
BASE COUNT     1228 a      1535 c      1574 g      1328 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaaggcaac gagaatacca ggagaaggag atccagcggc tcaataaggc cctggaggag 
      121 gccctcagca tccaggcctc tccatcatct ccgcctgcag cttttgggag tccagaaggc 
      181 gttgggggcc atctgaggaa gcaggaacta gtgacacaga atgagttgct gaaacagcag 
      241 gtaaagatct ttgaagagga cttccagagg gaacggagtg accgtgaacg catgaatgaa 
      301 gagaaggagg agctgaagaa gcaagtagag aagctgcagg cccaggtcac cctgactaat 
      361 gcccagctca aaactctcaa agaggaggag aaggccaagg aagccctcaa acagcagaag 
      421 aggaaagcaa aggcttcggg agagcgctac cacatggaac cccaccctga gcacgtctgc 
      481 ggcgcctatc cctatgccta cccacccatg ccagccatgg tacctcacca tgcctacaag 
      541 gactggtccc agatccgata ccctccaccc cctgtgccca tggagcaccc gcccccacac 
      601 cccaactctc gcctcttcca tctgccggag tacacctggc gtccaccctg tgcagggatt 
      661 cggaatcaga gctctcaagt gatggacccg cccccagaca ggcctgcaga gccagagtct 
      721 gcagacaatg actgtgatgg gccccagtga ggctgcagtg ggtcatttgg ttccaccttc 
      781 atctttcaga gccagctgac ctcagattgc caaaagtttg aaggccatgt gcatgttctg 
      841 tgtgacccaa gccttggcag aggagaggct gggatgggta gctggctcac atccccagcc 
      901 aagcctcgaa ctgttgacaa gacccccaat gccctggctc acaaatacca ctgagatctt 
      961 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc 
     1021 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc 
     1081 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt 
     1141 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat 
     1201 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga 
     1261 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt 
     1321 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct 
     1381 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc 
     1441 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     1501 aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt aattgcgttg 
     1561 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt 
     1621 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 
     1681 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     1741 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 
     1801 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     1861 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     1921 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga 
     1981 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     2041 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     2101 tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     2161 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     2221 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     2281 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     2341 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     2401 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     2461 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     2521 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     2581 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 
     2641 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     2701 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     2761 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     2821 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     2881 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     2941 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     3001 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     3061 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     3121 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     3181 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     3241 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     3301 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     3361 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     3421 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     3481 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     3541 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     3601 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     3661 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     3721 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     3781 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     3841 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     3901 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat 
     3961 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 
     4021 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 
     4081 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 
     4141 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 
     4201 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 
     4261 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc 
     4321 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt 
     4381 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg 
     4441 ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg 
     4501 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg 
     4561 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc 
     4621 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta 
     4681 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt 
     4741 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc 
     4801 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc 
     4861 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt 
     4921 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg 
     4981 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt 
     5041 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac 
     5101 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc 
     5161 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc 
     5221 tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg 
     5281 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat 
     5341 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa 
     5401 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg 
     5461 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg 
     5521 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc 
     5581 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt 
     5641 gctgtctcat cattttggca aagaa 
//
