LOCUS       pCAGGS-E-mABIN-1(420-560)        5232 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5367.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5367
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|449840789|
COMMENT     VNTNAME|pCAGGS-E-mABIN-1(420-560)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 988 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Eco47III" "agcgct" 4 0 3 446 0 4300 0 4462 0 0 
COMMENT            "") 
COMMENT        #3=(CRSite (CStringList) "TaqI" "tcga" 2 0 6 974 0 1790 0 3234 0 3504 0 
COMMENT            3887 0 4831 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 2953 0 0 "")) 
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COMMENT        #7=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 614 970 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1378 1452 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1378 1452 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1567 1968 1 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 1048 1130 1 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 1272 1347 1 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "intron AG promoter" 15 0 0 4257 5181 1 
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COMMENT        #14=(CFSignal (CObList) "AG promoter" 29 0 0 3888 5229 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5934..7275") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3505 3882 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #16=(CFSignal (CObList) "ampicillin" 4 0 1 2509 3369 1 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "mABIN-1(420-560)" 4 0 0 64 489 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "\337-globin polyA" 43 0 0 522 613 0 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 06/22/07 11:46" 1) 
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COMMENT                                        "Mouse A20-binding inhibitor of NF-kappa B activation 1 cDNA; aa 420-560" 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     misc_feature    614..970
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      complement(1378..1452)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1378..1452
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(1567..1968)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      522..613
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        complement(1048..1130)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="Escherichia coli lac operon promoter"
     rep_origin      1272..1347
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     intron          4257..5181
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3888..5229
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="Chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3505..3882
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     CDS             complement(2509..3369)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     CDS             64..489
                     /vntifkey="4"
                     /label=mABIN-1(420-560)
                     /note="Mouse A20-binding inhibitor of NF-kappa B activation 1 cDNA; aa 420-560"
BASE COUNT     1135 a      1387 c      1468 g      1242 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaaggcaac gagaatacca ggagaaggag atccagcggc tcaataaggc cctggaggag 
      121 gccctcagca tccaggcctc tccatcatct ccgcctgcag cttttgggag tccagaaggc 
      181 gttgggggcc atctgaggaa gcaggaacta gtgacacaga atgagttgct gaaacagcag 
      241 gtaaagatct ttgaagagga cttccagagg gaacggagtg accgtgaacg catgaatgaa 
      301 gagaaggagg agctgaagaa gcaagtagag aagctgcagg cccaggtcac cctgactaat 
      361 gcccagctca aaactctcaa agaggaggag aaggccaagg aagccctcaa acagcagaag 
      421 aggaaagcaa aggcttcggg agagcgctac cacatggaac cccaccctga gcacgtctgc 
      481 ggcgcctgac ccccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca 
      541 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat 
      601 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg 
      661 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg 
      721 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac 
      781 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt 
      841 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt 
      901 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct 
      961 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct 
     1021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
     1081 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     1141 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat 
     1201 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     1261 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     1321 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac 
     1381 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     1441 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     1501 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     1561 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     1621 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc 
     1681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     1741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     1801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     1861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     1921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     1981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     2041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     2101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     2161 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 
     2221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     2281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     2341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     2401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     2461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     2521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     2581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     2641 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     2701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     2761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     2821 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     2881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     2941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     3001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     3061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     3121 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     3181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     3241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     3301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     3361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     3421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     3481 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa 
     3541 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 
     3601 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     3661 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     3721 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     3781 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     3841 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 
     3901 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 
     3961 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg 
     4021 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc 
     4081 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 
     4141 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct 
     4201 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga 
     4261 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg 
     4321 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg 
     4381 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg 
     4441 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg 
     4501 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga 
     4561 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg 
     4621 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg 
     4681 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc 
     4741 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg 
     4801 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 
     4861 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 
     4921 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 
     4981 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 
     5041 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 
     5101 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 
     5161 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 
     5221 tttggcaaag aa 
//
