LOCUS pCAGGS-E-mABIN-1(444-647) 5593 bp DNA circular 1-OCT-2007 DEFINITION LMBP 5369. SOURCE ORGANISM COMMENT Accession number: LMBP 5369 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 01-OCT-2007 COMMENT VNTDATE|457287421| COMMENT VNTNAME|pCAGGS-E-mABIN-1(444-647)| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pCAGGS-E-mABIN-1(444-647)" 0 5593 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 COMMENT 0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) COMMENT (CWordArray) (CStringList "BsrDI" "CaiI" "NcoI" "NotI" "PvuI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") COMMENT (CObList COMMENT #0=(COligo "pMB1 ori" COMMENT "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGC" COMMENT "Fragment of pRH007; Length: 403" 0 (CStringList) 0)) 1 "{(0,1),2}" COMMENT 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 460 459 1 5593 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #1=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 969 1 3005 0 3179 1 5213 COMMENT 1 0 "") COMMENT #2=(CRSite (CStringList) "CaiI" "cagnnnctg" 7 0 3 647 0 2467 0 5546 0 0 COMMENT "") COMMENT #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 12 0 444 0 507 0 1626 0 COMMENT 4242 0 0 "") COMMENT #4=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") COMMENT #5=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3314 0 0 "")) COMMENT (CObList COMMENT #6=(CFSignal (CObList) "mABIN-1 (444-647)" 4 0 0 64 678 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "64..678") COMMENT #7=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "16..54") COMMENT #8=(CFSignal (CObList) "ampicillin" 4 0 1 2870 3730 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2870..3730)") COMMENT #9=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3866 4243 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3866..4243") COMMENT #10=(CFSignal (CObList) "AG promoter" 29 0 0 4249 5590 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4249..5590") COMMENT #11=(CFSignal (CObList) "intron AG promoter" 15 0 0 4618 5542 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4618..5542") COMMENT #12=(CFSignal (CObList) "SV40 ori" 33 0 0 1633 1708 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1633..1708") COMMENT #13=(CFSignal (CObList) "lac promoter" 30 0 1 1409 1491 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1409..1491)") COMMENT #14=(CFSignal (CObList) "\337-globin polyA" 43 0 0 883 974 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "883..974") COMMENT #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 1928 2329 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1928..2329)") COMMENT #16=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1739 1813 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1739..1813") COMMENT #17=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1739 1813 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(1739..1813)") COMMENT #18=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 975 1331 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "975..1331")) (CObList) COMMENT (CObList) (CObList) (CObList) COMMENT (CObList COMMENT #19=(CFSignal (CObList) "LMBP 5369" 2 0 0 0 0 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #20=(CFSignal (CObList) "Last modified: 01-OCT-2007" 2 0 0 0 0 0 COMMENT (CStringList) (CStringList) 1 1 0 0 "")) (CObList) COMMENT (CTextView 0 COMMENT #21=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #22=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) COMMENT "pCAGGS-E-mABIN-1(444-647)" 2) 5 "" 0 4) COMMENT #23=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) COMMENT (CObjectList COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'pCAGGS-E-mABIN-1(444-647)'" 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5369." COMMENT 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. 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Marker (1 total)") COMMENT #74=(CFolderPar COMMENT (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #75=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3866 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3866 End: 4243" 1) COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only" COMMENT 1) COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3866..4243" 1))) "hCMV-IE enhancer") COMMENT #80=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4249 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4249 End: 5590" 1) COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Chicken 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COMMENT "Promoter Prokaryotic (1 total)") COMMENT #91=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #92=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1633 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1633 End: 1708" 1) COMMENT #94=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "bidirectional origin of SV40" 1) COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #96=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1633..1708" 1))) "SV40 ori") COMMENT #97=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1928 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #98=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1928 End: 2329 (Complementary)" COMMENT 1) COMMENT #99=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "origin plasmid" 1) 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COMMENT #125=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 30615904 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #126=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #127=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 647 " 1) COMMENT #128=(CLinePar COMMENT (CParagraph 0 (1 #2# 2) 1 2 2 0 191) COMMENT " N2: 2467 " 1) COMMENT #129=(CLinePar COMMENT (CParagraph 0 (1 #2# 3) 1 2 2 0 191) COMMENT " N3: 5546 " 1))))) "CaiI: 3 sites") COMMENT 6 3 "CAGNNNCTG" "GTCNNNGAC") COMMENT #130=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 30614944 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #131=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #132=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 12 " 1) COMMENT #133=(CLinePar COMMENT (CParagraph 0 (1 #3# 2) 1 2 2 0 191) COMMENT " N2: 444 " 1) COMMENT #134=(CLinePar COMMENT (CParagraph 0 (1 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Location/Qualifiers CDS 64..678 /vntifkey="4" /label=mABIN-1\(444-647) /note="Mouse A20-binding inhibitor of NF-kappaB activation 1 cDNA; aa 444-647" misc_marker 16..54 /vntifkey="22" /label=E-tag /note="E-tag" CDS complement(2870..3730) /vntifkey="4" /label=ampicillin /note="antibiotic resistance gene; coding sequence" promoter 3866..4243 /vntifkey="29" /label=hCMV-IE\enhancer /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only" promoter 4249..5590 /vntifkey="29" /label=AG\promoter /note="Chicken ß-actin/rabbit ß-globin hybrid promoter" intron 4618..5542 /vntifkey="15" /label=intron\AG\promoter /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter" rep_origin 1633..1708 /vntifkey="33" /label=SV40\ori /note="bidirectional origin of SV40" promoter complement(1409..1491) /vntifkey="30" /label=lac\promoter /note="Escherichia coli lac operon promoter" terminator 883..974 /vntifkey="43" /label=ß-globin\polyA /note="3' UTR of the rabbit ß-globin gene, incl. polyA" rep_origin complement(1928..2329) /vntifkey="33" /label=pMB1\ori /note="origin plasmid" terminator 1739..1813 /vntifkey="43" /label=SV40\polyA\early /note="polyA signal of the SV40 early region" terminator complement(1739..1813) /vntifkey="43" /label=SV40\polyA\late /note="polyA signal of the SV40 late region" misc_feature 975..1331 /vntifkey="21" /label=3'\FR\of\ß-globin /note="3' flanking region of the rabbit ß-globin gene" BASE COUNT 1205 a 1516 c 1551 g 1321 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gcagcctctc catcatctcc gcctgcagct tttgggagtc cagaaggcgt tgggggccat 121 ctgaggaagc aggaactagt gacacagaat gagttgctga aacagcaggt aaagatcttt 181 gaagaggact tccagaggga acggagtgac cgtgaacgca tgaatgaaga gaaggaggag 241 ctgaagaagc aagtagagaa gctgcaggcc caggtcaccc tgactaatgc ccagctcaaa 301 actctcaaag aggaggagaa ggccaaggaa gccctcaaac agcagaagag gaaagcaaag 361 gcttcgggag agcgctacca catggaaccc caccctgagc acgtctgcgg cgcctatccc 421 tatgcctacc cacccatgcc agccatggta cctcaccatg cctacaagga ctggtcccag 481 atccgatacc ctccaccccc tgtgcccatg gagcacccgc ccccacaccc caactctcgc 541 ctcttccatc tgccggagta cacctggcgt ccaccctgtg cagggattcg gaatcagagc 601 tctcaagtga tggacccgcc cccagacagg cctgcagagc cagagtctgc agacaatgac 661 tgtgatgggc cccagtgagg ctgcagtggg tcatttggtt ccaccttcat ctttcagagc 721 cagctgacct cagattgcca aaagtttgaa ggccatgtgc atgttctgtg tgacccaagc 781 cttggcagag gagaggctgg gatgggtagc tggctcacat ccccagccaa gcctcgaact 841 gttgacaaga cccccaatgc cctggctcac aaataccact gagatctttt tccctctgcc 901 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa 961 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat 1021 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat 1081 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa 1141 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt 1201 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt 1261 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc 1321 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc 1381 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 1441 taaagcctgg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 1501 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca 1561 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 1621 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 1681 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa 1741 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 1801 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 1861 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 1921 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 1981 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg 2041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 2101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 2161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 2221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 2281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 2341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 2401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 2461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 2521 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 2581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 2641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 2701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 2761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 2821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 2881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 2941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 3001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 3061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 3121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 3181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 3241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 3301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 3361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 3421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 3481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 3541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 3601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 3661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 3721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 3781 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 3841 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca 3901 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 3961 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 4021 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 4081 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 4141 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 4201 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc 4261 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt 4321 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc 4381 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat 4441 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat 4501 aaaaagcgaa gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc 4561 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 4621 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 4681 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 4741 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 4801 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 4861 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 4921 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 4981 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 5041 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 5101 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg 5161 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct 5221 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc 5281 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc 5341 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct 5401 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag 5461 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca 5521 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca 5581 ttttggcaaa gaa //