LOCUS       pcI857                  4182 bp    DNA     circular     24-MAR-2011
DEFINITION  LMBP 537.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 537
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-MAR-2011
            
            
COMMENT     VNTNAME|pcI857|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pcI857" 0 4182 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AgeI" "AvaI" "BglII" "ClaI" "PvuI" "XhoI" "HindII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 2040 0 2364 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 2 2814 0 3088 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BglII" "agatct" 2 0 1 59 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 2907 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3216 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2814 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindII" "gtyrac" 4 0 2 177 0 733 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "lambda cl857" 4 0 0 226 939 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "226..939") 
COMMENT        #8=(CFSignal (CObList) "p15A ori" 33 0 0 1765 2264 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1765..2264") 
COMMENT        #9=(CFSignal (CObList) "Tn903 neomycin resistance" 4 0 0 2784 3599 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2784..3599") 
COMMENT        #10=(CFSignal (CObList) "lambda PR promoter" 30 0 1 144 210 0 
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COMMENT        #11=(CFSignal (CObList) "lambda PRM promoter" 30 0 0 170 225 0 
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COMMENT        #13=(CFSignal (CObList) "Last modified: 24-MAR-2011" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pcI857'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 03/24/11 03:53" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4182 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 22-MAR-2011" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "phage lambda thermosensitive repressor" 
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COMMENT                                        "phage lambda major rightward promoter" 
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COMMENT                          "ACCGGT" "TGGCCA") 
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COMMENT                          3 "GTYRAC" "CARYTG"))) "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     promoter        170..225
                     /vntifkey="30"
                     /label=lambda\PRM\promoter
                     /note="phage lambda promoter for repressor maintenance"
     promoter        complement(144..210)
                     /vntifkey="30"
                     /label=lambda\PR\promoter
                     /note="phage lambda major rightward promoter"
     CDS             226..939
                     /vntifkey="4"
                     /label=lambda\cl857
                     /note="phage lambda thermosensitive repressor"
     rep_origin      1765..2264
                     /vntifkey="33"
                     /label=p15A\ori
     CDS             2784..3599
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
BASE COUNT     1108 a       927 c      1041 g      1106 t 
ORIGIN
        1 tctcgatgat ggttacgcca gactatcaaa tatgctgctt gaggcttatt cgggcgcaga 
       61 tctttagctg tcttggtttg cccaaagcgc attgcataat ctttcagggt tatgcgttgt 
      121 tccatacaac ctccttagta catgcaacca ttatcaccgc cagaggtaaa atagtcaaca 
      181 cgcacggtgt tagatattta tcccttgcgg tgatagattt aacgtatgag cacaaaaaag 
      241 aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa 
      301 aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag 
      361 tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg 
      421 cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc 
      481 tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct 
      541 gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt 
      601 gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag 
      661 gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg 
      721 ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt 
      781 gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa 
      841 ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa 
      901 gttatcgcta gtcagtggcc tgaagagacg tttggctgat cggcaaggtg ttctggtcgg 
      961 cgcatagctg ataacaattg agcaagaatc ttcatcgaat taggggaatt ttcactcccc 
     1021 tcagaacata acatagtaaa tggattgaat tatgaagaat ggtttttatg cgacttaccg 
     1081 cagcaaaaat aaagggaaag ataagcgctc aataaacctg tctgttttcc ttaattctct 
     1141 gctggctgat aatcatcacc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc 
     1201 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 
     1261 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 
     1321 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 
     1381 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 
     1441 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 
     1501 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 
     1561 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 
     1621 gaccccttaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct 
     1681 gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc taccaactct 
     1741 ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct ttcagtttag 
     1801 ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag tggctgctgc 
     1861 cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac cggataaggc 
     1921 gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta 
     1981 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac 
     2041 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg ggaaacgcct 
     2101 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat 
     2161 gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc tcacttccct 
     2221 gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag ccatttccgc 
     2281 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatatcct 
     2341 gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac atgaagcact 
     2401 tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc tagcgctgag 
     2461 gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca 
     2521 gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga 
     2581 ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat 
     2641 ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct caaaatctct 
     2701 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca 
     2761 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc 
     2821 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg 
     2881 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt 
     2941 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa 
     3001 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg 
     3061 atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat 
     3121 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt 
     3181 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc 
     3241 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct 
     3301 ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag 
     3361 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag 
     3421 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat 
     3481 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta 
     3541 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat 
     3601 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg 
     3661 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga 
     3721 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa 
     3781 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc 
     3841 ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgctcaa agatgcaggg 
     3901 gtaaaagcta accgcatctt taccgacaag gcatccggca gttcaacaga tcgggaaggg 
     3961 ctggatttgc tgaggatgaa ggtggaggaa ggtgatgtca ttctggtgaa gaagctcgac 
     4021 cgtcttggcc gcgacacgcc gacatgatcc aactgataaa agagtttgat gctcagggtg 
     4081 tagcggttcg gtttattgac gacgggatca gtaccgacgg tgatatgggg caaatggtgg 
     4141 tcaccatcct gtcggctgtg gcacaggctg aacgccggag ga 
//
