LOCUS       pCAGGS-E-mABIN-1(469-647)        5518 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5372.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5372
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|457353348|
COMMENT     VNTNAME|pCAGGS-E-mABIN-1(469-647)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "CaiI" "cagnnnctg" 7 0 3 572 0 2392 0 5471 0 0 
COMMENT            "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 12 0 369 0 432 0 1551 0 
COMMENT            4167 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 894 1 2930 0 3104 1 5138 
COMMENT            1 0 "") #4=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3239 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "")) 
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COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1664 1738 1 
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COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1664 1738 1 
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COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1853 2254 1 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "\337-globin polyA" 43 0 0 808 899 1 
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COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 1334 1416 1 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "SV40 ori" 33 0 0 1558 1633 1 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "ampicillin" 4 0 1 2795 3655 1 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "mABIN-1(469-647)" 4 0 0 64 603 0 (CStringList) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 09/13/07 10:35" 1) 
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COMMENT                          "Modification Date in the Original DB: 13-SEP-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Mouse A20-binding inhibitor of NF-kappa B activation 1 cDNA; aa 469-647" 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     misc_feature    900..1256
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      complement(1664..1738)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1664..1738
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(1853..2254)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      808..899
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        complement(1334..1416)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="Escherichia coli lac operon promoter"
     rep_origin      1558..1633
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     intron          4543..5467
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4174..5515
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="Chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3791..4168
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only"
     CDS             complement(2795..3655)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     CDS             64..603
                     /vntifkey="4"
                     /label=mABIN-1(469-647)
                     /note="Mouse A20-binding inhibitor of NF-kappa B activation 1 cDNA; aa 469-647"
BASE COUNT     1190 a      1495 c      1528 g      1305 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcagtgacac agaatgagtt gctgaaacag caggtaaaga tctttgaaga ggacttccag 
      121 agggaacgga gtgaccgtga acgcatgaat gaagagaagg aggagctgaa gaagcaagta 
      181 gagaagctgc aggcccaggt caccctgact aatgcccagc tcaaaactct caaagaggag 
      241 gagaaggcca aggaagccct caaacagcag aagaggaaag caaaggcttc gggagagcgc 
      301 taccacatgg aaccccaccc tgagcacgtc tgcggcgcct atccctatgc ctacccaccc 
      361 atgccagcca tggtacctca ccatgcctac aaggactggt cccagatccg ataccctcca 
      421 ccccctgtgc ccatggagca cccgccccca caccccaact ctcgcctctt ccatctgccg 
      481 gagtacacct ggcgtccacc ctgtgcaggg attcggaatc agagctctca agtgatggac 
      541 ccgcccccag acaggcctgc agagccagag tctgcagaca atgactgtga tgggccccag 
      601 tgaggctgca gtgggtcatt tggttccacc ttcatctttc agagccagct gacctcagat 
      661 tgccaaaagt ttgaaggcca tgtgcatgtt ctgtgtgacc caagccttgg cagaggagag 
      721 gctgggatgg gtagctggct cacatcccca gccaagcctc gaactgttga caagaccccc 
      781 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac 
      841 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca 
      901 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat 
      961 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct 
     1021 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc 
     1081 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt 
     1141 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt 
     1201 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc 
     1261 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 
     1321 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc 
     1381 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     1441 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa 
     1501 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     1561 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 
     1621 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc 
     1681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     1741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg 
     1801 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     1861 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     1921 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt 
     1981 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     2041 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     2101 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     2161 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     2221 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     2281 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     2341 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     2401 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     2461 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     2521 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     2581 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     2641 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     2701 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     2761 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     2821 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     2881 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     2941 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     3001 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     3061 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     3121 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     3181 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     3241 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     3301 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     3361 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     3421 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     3481 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     3541 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     3601 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     3661 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     3721 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     3781 cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta 
     3841 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 
     3901 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 
     3961 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 
     4021 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 
     4081 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 
     4141 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact 
     4201 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt 
     4261 tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc ggggcggggc 
     4321 gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc 
     4381 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg 
     4441 cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg 
     4501 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc 
     4561 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg 
     4621 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg 
     4681 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc 
     4741 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc 
     4801 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg 
     4861 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc 
     4921 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt 
     4981 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg 
     5041 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg gcggcccccg 
     5101 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg 
     5161 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg 
     5221 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat 
     5281 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg 
     5341 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt 
     5401 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt 
     5461 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaa 
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