LOCUS       pCAGGS-E-mABIN-1(469-601)        5208 bp    DNA     circular     24-OCT-2007
DEFINITION  LMBP 5374.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5374
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 24-OCT-2007
            
COMMENT     VNTDATE|454689894|
COMMENT     VNTNAME|pCAGGS-E-mABIN-1(469-601)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        (CWordArray) (CStringList "BglII" "HindIII" "NotI" "PvuI" "TaqI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 964 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BglII" "agatct" 2 0 2 99 0 498 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "TaqI" "tcga" 2 0 6 950 0 1766 0 3210 0 3480 0 
COMMENT            3863 0 4807 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 2929 0 0 "")) 
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COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 33 0 1 1543 1944 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "lac promoter" 30 0 1 1024 1106 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3481 3858 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #9=(CFSignal (CObList) "ampicillin" 4 0 1 2485 3345 1 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "\337-globin polyA" 43 0 0 498 589 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 590 946 1 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 08/14/07 02:44" 1) 
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COMMENT                          "Last Modified: 10/24/07 11:58" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     misc_feature    590..946
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the ß-globin gene"
     terminator      complement(1354..1428)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1354..1428
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(1543..1944)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      498..589
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        complement(1024..1106)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1248..1323
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     intron          4233..5157
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     promoter        3864..5205
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3481..3858
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     CDS             complement(2485..3345)
                     /vntifkey="4"
                     /label=ampicillin
     CDS             64..465
                     /vntifkey="4"
                     /label=mABIN-1(469-601)
BASE COUNT     1123 a      1405 c      1440 g      1240 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcagtgacac agaatgagtt gctgaaacag caggtaaaga tctttgaaga ggacttccag 
      121 agggaacgga gtgaccgtga acgcatgaat gaagagaagg aggagctgaa gaagcaagta 
      181 gagaagctgc aggcccaggt caccctgact aatgcccagc tcaaaactct caaagaggag 
      241 gagaaggcca aggaagccct caaacagcag aagaggaaag caaaggcttc gggagagcgc 
      301 taccacatgg aaccccaccc tgagcacgtc tgcggcgcct atccctatgc ctacccaccc 
      361 atgccagcca tggtacctca ccatgcctac aaggactggt cccagatccg ataccctcca 
      421 ccccctgtgc ccatggagca cccgccccca caccccaact cttgaccccc aatgccctgg 
      481 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc 
      541 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt 
      601 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc 
      661 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca 
      721 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat 
      781 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt 
      841 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct 
      901 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc 
      961 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     1021 attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc taatgagtga 
     1081 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     1141 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 
     1201 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 
     1261 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 
     1321 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt 
     1381 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     1441 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat 
     1501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
     1561 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     1621 cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt gagcaaaagg 
     1681 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     1741 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     1801 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     1861 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     1921 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     1981 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     2041 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     2101 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     2161 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     2221 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     2281 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     2341 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     2401 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     2461 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     2521 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     2581 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     2641 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     2701 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     2761 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 
     2821 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     2881 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     2941 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     3001 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     3061 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 
     3121 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     3181 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     3241 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     3301 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     3361 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     3421 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc 
     3481 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 
     3541 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 
     3601 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 
     3661 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 
     3721 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 
     3781 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 
     3841 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct 
     3901 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga 
     3961 tgggggcggg gggggggggg gggccccccc caggcggggc ggggcggggc gaggggcggg 
     4021 gcggggcgag gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc 
     4081 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg 
     4141 gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg cgccgcccgc 
     4201 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc 
     4261 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa 
     4321 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg 
     4381 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct 
     4441 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg 
     4501 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt 
     4561 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc 
     4621 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg 
     4681 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg 
     4741 ggccgcctcg ggccggggag ggctcggggg aaggggcgcg gcggcccccg gagcgccggc 
     4801 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg 
     4861 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc 
     4921 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag 
     4981 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg 
     5041 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg 
     5101 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc 
     5161 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaa 
//
