LOCUS       pCAGGS-E-mABIN-1(444-524)        5052 bp    DNA     circular     24-SEP-2007
DEFINITION  LMBP 5375.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5375
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-SEP-2007
COMMENT     VNTDATE|457276829|
COMMENT     VNTNAME|pCAGGS-E-mABIN-1(444-524)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-mABIN-1(444-524)" 0 5052 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "BcuI" "HindIII" "NotI" "PvuI" "TaqI" "EcoRI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5052 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 808 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 2773 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "TaqI" "tcga" 2 0 6 794 0 1610 0 3054 0 3324 0 
COMMENT            3707 0 4651 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "16..54") 
COMMENT        #7=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 434 790 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1198 1272 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1198 1272 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1387 1788 1 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "\337-globin polyA" 43 0 0 342 433 1 
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COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 868 950 1 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "intron AG promoter" 15 0 0 4077 5001 1 
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COMMENT        #15=(CFSignal (CObList) "AG promoter" 29 0 0 3708 5049 1 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3325 3702 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #17=(CFSignal (CObList) "ampicillin" 4 0 1 2329 3189 1 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "mABIN-1 (444-524)" 4 0 0 64 309 0 (CStringList) 
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COMMENT                          1) 
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COMMENT                          "Created: 09/12/07 01:20" 1) 
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COMMENT                          "Last Modified: 09/24/07 09:45" 1) 
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COMMENT                                        "Mouse A20-binding inhibitor of NF-kappaB activation 1 cDNA (mABIN-1); aa 444-524" 
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COMMENT                                 "\337-globin polyA") 
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COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     misc_feature    434..790
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      complement(1198..1272)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1198..1272
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(1387..1788)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      342..433
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        complement(868..950)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1092..1167
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     intron          4077..5001
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3708..5049
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3325..3702
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     CDS             complement(2329..3189)
                     /vntifkey="4"
                     /label=ampicillin
     CDS             64..309
                     /vntifkey="4"
                     /label=mABIN-1\(444-524)
                     /note="Mouse A20-binding inhibitor of NF-kappaB activation 1 cDNA (mABIN-1); aa 444-524"
BASE COUNT     1076 a      1338 c      1411 g      1227 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcagcctctc catcatctcc gcctgcagct tttgggagtc cagaaggcgt tgggggccat 
      121 ctgaggaagc aggaactagt gacacagaat gagttgctga aacagcaggt aaagatcttt 
      181 gaagaggact tccagaggga acggagtgac cgtgaacgca tgaatgaaga gaaggaggag 
      241 ctgaagaagc aagtagagaa gctgcaggcc caggtcaccc tgactaatgc ccagctcaaa 
      301 actctctgac ccccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca 
      361 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat 
      421 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg 
      481 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg 
      541 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac 
      601 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt 
      661 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt 
      721 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct 
      781 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct 
      841 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
      901 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
      961 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat 
     1021 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     1081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     1141 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac 
     1201 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     1261 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     1321 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     1381 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     1441 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc 
     1501 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     1561 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     1621 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     1681 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     1741 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     1801 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     1861 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     1921 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     1981 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 
     2041 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     2101 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     2161 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     2221 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     2281 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     2341 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     2401 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     2461 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     2521 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     2581 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     2641 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     2701 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     2761 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     2821 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     2881 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     2941 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     3001 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     3061 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     3121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     3181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     3241 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     3301 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa 
     3361 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 
     3421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     3481 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     3541 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     3601 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     3661 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 
     3721 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 
     3781 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg 
     3841 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc 
     3901 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 
     3961 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct 
     4021 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga 
     4081 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg 
     4141 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg 
     4201 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg 
     4261 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg 
     4321 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga 
     4381 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg 
     4441 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg 
     4501 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc 
     4561 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg 
     4621 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 
     4681 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 
     4741 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 
     4801 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 
     4861 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 
     4921 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 
     4981 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 
     5041 tttggcaaag aa 
//
