LOCUS       pCAGGS-HA-mABIN-1(54-647)        6765 bp    DNA     circular     26-OCT-2007
DEFINITION  LMBP 5388.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5388
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 26-OCT-2007
            
COMMENT     VNTDATE|450455924|
COMMENT     VNTNAME|pCAGGS-HA-mABIN-1(54-647)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT            0 5414 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 511 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 6 613 0 2844 0 4236 0 
COMMENT            5144 0 5266 0 5337 0 0 "")) 
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COMMENT            (CStringList) 1 1 1 1 "5957..5983") 
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COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5038 5415 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #7=(CFSignal (CObList) "SV40 ori" 33 0 0 2805 2880 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 2581 2663 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "\337-globin polyA" 43 0 0 2055 2146 0 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 06/29/07 02:38" 1) 
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FEATURES             Location/Qualifiers
     misc_marker     15..41
                     /vntifkey="22"
                     /label=HA\epitope
     CDS             complement(4042..4902)
                     /vntifkey="4"
                     /label=ampicillin
     promoter        5038..5415
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        5421..6762
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken  ß-actin/rabbit  ß-globin hybrid promoter"
     intron          5790..6714
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken  ß-actin/rabbit  ß-globin hybrid intron"
     rep_origin      2805..2880
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        complement(2581..2663)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      2055..2146
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     rep_origin      complement(3100..3501)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      2911..2985
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2911..2985)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     misc_feature    2147..2503
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin"
     CDS             66..1850
                     /vntifkey="4"
                     /label=mABIN-1(54-647)
BASE COUNT     1528 a      1845 c      1897 g      1495 t 
ORIGIN
        1 ttgaattcat ggcttaccca tacgatgttc cagattacgc tagcttgggt ggtcatatgg 
       61 ccgcgatgga agcgtccaga ctccggcaga aggcagagga gctggtcaag gacagcgagc 
      121 tgtcaccacc gacatctgcc ccctccttgg tctcctttga tgacctggct gagctcacag 
      181 gacaggatac aaaggtccag gtacatcctg ctaccagcac tgccgccacc accaccgcca 
      241 ccgccaccac gggaaactcc atggagaagc ccgagccagc ctccaaatct ccgtccaatg 
      301 gcgcctcctc ggactttgaa gtggtcccta ctgaggagca gaattcaccc gaaactggca 
      361 gccaccctac gaacatgatg gacctggggc ccccaccccc agaggacagc aacctgaagc 
      421 tccacctgca gcgcctggag accaccctta gcgtgtgtgc agaggagcca gaccacagcc 
      481 agctcttcac ccacctgggc cgcatggccc tcgagttcaa caggttggcc tccaaagtgc 
      541 ataaaaatga gcagcgcacc tccatcctgc agaccttatg tgagcagctg cgccaggaga 
      601 atgaagccct gaaggccaag ctggacaagg gcctggaaca gcgggatctg gctgctgaga 
      661 ggctgcggga ggaaaacacg gagctcaaga aactgttgat gaacagcagc tgcaaagagg 
      721 gactctgtgg gcagcccagc tccccaaagc cagagggtgc tggcaagaag ggcgtggctg 
      781 gacagcagca ggccagtgtg atggcgagta aagtccctga agcgggggcc tttggagcag 
      841 ctgagaagaa agtgaagttg ctagaacagc aacgcatgga gctgctggaa gtgaacaagc 
      901 agtgggacca gcatttccgg tccatgaagc agcagtatga gcagaagatc acagagcttc 
      961 gccagaagct ggtggacctg cagaaacagg taactgagct ggaggccgaa cgggagcaga 
     1021 agcagcgtga ctttgaccgg aaactcctcc tggccaaatc gaagatagag atggaagaga 
     1081 ccgacaagga gcagctgaca gcagaggcca aggaactgcg ccagaaggtc aggtacctac 
     1141 aggatcagct gagcccgctc acaaggcaac gagaatacca ggagaaggag atccagcggc 
     1201 tcaataaggc cctggaggag gccctcagca tccaggcctc tccatcatct ccgcctgcag 
     1261 cttttgggag tccagaaggc gttgggggcc atctgaggaa gcaggaacta gtgacacaga 
     1321 atgagttgct gaaacagcag gtaaagatct ttgaagagga cttccagagg gaacggagtg 
     1381 accgtgaacg catgaatgaa gagaaggagg agctgaagaa gcaagtagag aagctgcagg 
     1441 cccaggtcac cctgactaat gcccagctca aaactctcaa agaggaggag aaggccaagg 
     1501 aagccctcaa acagcagaag aggaaagcaa aggcttcggg agagcgctac cacatggaac 
     1561 cccaccctga gcacgtctgc ggcgcctatc cctatgccta cccacccatg ccagccatgg 
     1621 tacctcacca tgcctacaag gactggtccc agatccgata ccctccaccc cctgtgccca 
     1681 tggagcaccc gcccccacac cccaactctc gcctcttcca tctgccggag tacacctggc 
     1741 gtccaccctg tgcagggatt cggaatcaga gctctcaagt gatggacccg cccccagaca 
     1801 ggcctgcaga gccagagtct gcagacaatg actgtgatgg gccccagtga ggctgcagtg 
     1861 ggtcatttgg ttccaccttc atctttcaga gccagctgac ctcagattgc caaaagtttg 
     1921 aaggccatgt gcatgttctg tgtgacccaa gccttggcag aggagaggct gggatgggta 
     1981 gctggctcac atccccagcc aagcctcgaa ctgttgacaa gacccccaat gccctggctc 
     2041 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc 
     2101 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga 
     2161 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga 
     2221 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag 
     2281 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc 
     2341 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt 
     2401 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct 
     2461 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca 
     2521 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
     2581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct 
     2641 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     2701 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 
     2761 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
     2821 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 
     2881 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac 
     2941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     3001 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa 
     3061 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     3121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     3181 taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca 
     3241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     3301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     3361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     3421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     3481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     3541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     3601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     3661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     3721 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     3781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     3841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     3901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     3961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     4021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     4081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     4141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     4201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     4261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     4321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     4381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     4441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     4501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     4561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     4621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     4681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     4741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     4801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     4861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     4921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     4981 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac 
     5041 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 
     5101 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
     5161 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
     5221 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
     5281 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
     5341 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
     5401 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc 
     5461 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg 
     5521 gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg 
     5581 gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 
     5641 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag 
     5701 tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc 
     5761 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg 
     5821 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc 
     5881 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt 
     5941 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg 
     6001 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg 
     6061 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 
     6121 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc 
     6181 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg 
     6241 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc 
     6301 cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc 
     6361 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag 
     6421 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc 
     6481 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc 
     6541 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg 
     6601 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc 
     6661 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg 
     6721 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa 
//
