LOCUS       pAS2-mABIN-1           10524 bp    DNA     circular     29-JUN-2007
DEFINITION  LMBP 5392.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5392
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 29-JUN-2007
            
            
COMMENT     VNTDATE|441389640|
COMMENT     VNTNAME|pAS2-mABIN-1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAS2-mABIN-1" 0 10524 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BglI" "EcoRI" "NdeI" "NheI" "SacI" "SmaI" "SspI" "BsmI" 
COMMENT         "Bsp119I" "EaeI" "Eco32I" "PaeI" "Psp5II" "NotI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 10524 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 3 3134 0 6714 0 9728 0 
COMMENT            0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 3951 0 5946 0 6443 0 0 
COMMENT            "") #2=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 5998 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 5982 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 4 3298 0 6277 0 6787 0 7875 
COMMENT            0 0 "") #5=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 8127 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SspI" "aatatt" 4 0 4 880 0 2678 0 5443 0 10412 
COMMENT            0 0 "") #7=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 1 4302 1 0 "") 
COMMENT        #8=(CRSite (CStringList) "Bsp119I" "ttcgaa" 3 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "EaeI" "yggccr" 2 0 6 3271 0 4200 0 6001 0 7153 
COMMENT            0 8554 0 9996 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "Eco32I" "gatatc" 4 0 3 2169 0 3563 0 4769 0 0 
COMMENT             "") 
COMMENT        #11=(CRSite (CStringList) "PaeI" "gcatgc" 6 0 3 5077 0 5533 0 8354 0 0 
COMMENT             "") 
COMMENT        #12=(CRSite (CStringList) "Psp5II" "rggwccy" 3 0 3 3467 0 4567 0 6021 0 
COMMENT             0 "") #13=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "")) 
COMMENT       (CObList 
COMMENT        #14=(CFSignal (CObList) "FLP terminator" 43 0 1 1060 1164 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "CYC1 terminator" 43 0 1 3312 3586 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3312..3586)") 
COMMENT        #16=(CFSignal (CObList) "2mu ori" 33 0 1 2 982 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2..982)") 
COMMENT        #17=(CFSignal (CObList) "TRP1" 4 0 0 1884 2558 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1884..2558") 
COMMENT        #18=(CFSignal (CObList) "f1 ori" 33 0 1 2661 3116 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2661..3116)") 
COMMENT        #19=(CFSignal (CObList) "ADH1 promoter" 29 0 0 5075 5478 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5075..5478") 
COMMENT        #20=(CFSignal (CObList) "GALbd" 4 0 0 5501 5941 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5501..5941") 
COMMENT        #21=(CFSignal (CObList) "HA epitope" 22 0 0 5957 5983 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5957..5983") 
COMMENT        #22=(CFSignal (CObList) "mABIN-1" 4 0 0 6008 7951 0 (CStringList) 
COMMENT             (CStringList "/gene=\"ABINl\"" "/codon_start=1" 
COMMENT              "/product=\"ABINl, A20-binding inhibitor of NF-kappa B" 
COMMENT              "activation (large)\"" "/protein_id=\"CAB44240.1\"" 
COMMENT              "/db_xref=\"GI:4995753\"" "/db_xref=\"GOA:Q9WUU8\"" 
COMMENT              "/db_xref=\"UniProtKB/Swiss-Prot:Q9WUU8\"") 1 1 1 1 "117..2060") 
COMMENT        #23=(CFSignal (CObList) "lac promoter" 30 0 1 8416 8499 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "ADH1 terminator" 43 0 0 8162 8263 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6051..6152") 
COMMENT        #25=(CFSignal (CObList) "pMB1 ori" 33 0 1 8591 8993 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6480..6882)") 
COMMENT        #26=(CFSignal (CObList) "ampicillin" 4 0 1 9534 10394 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7423..8283)") 
COMMENT        #27=(CFSignal (CObList) "CYH2" 4 0 1 3855 4304 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3855..4304)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #28=(CFSignal (CObList) "LMBP 5392" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #29=(CFSignal (CObList) "29-JUN-2007" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
COMMENT              #31=(CRefLinePar 
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COMMENT                   5 "" 0 4) 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pAS2-mABIN-1'" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5392" 
COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/20/07 04:14" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/29/07 02:48" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 10524 bp" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #41=(CFolderPar 
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COMMENT                   "Standard Fields") 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #45=(CRefLinePar 
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COMMENT                   0 0) 
COMMENT              #46=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 5392") 
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COMMENT                           (CObjectList 
COMMENT                            #50=(CLinePar (CParagraph 0 (15 #29# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "29-JUN-2007"))) "Annotations") 
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COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
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COMMENT                                        "Start: 1884  End: 2558" 1) 
COMMENT                                   #55=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1884..2558" 1))) "TRP1") 
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COMMENT                                        "Start: 3855  End: 4304 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3855..4304)" 1))) "CYH2") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5501  End: 5941" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5501..5941" 1))) "GALbd") 
COMMENT                            #65=(CFolderPar 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6008  End: 7951" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   117..2060" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /gene=\"ABINl\"" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /codon_start=1" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /product=\"ABINl, A20-binding inhibitor of NF-kappa B" 
COMMENT                                        1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   activation (large)\"" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /protein_id=<a href=\"ncbi:protein/CAB44240.1\">CAB44240.1</a>" 
COMMENT                                        1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /db_xref=<a href=\"ncbi:sequence/4995753\">GI:4995753</a>" 
COMMENT                                        1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /db_xref=<a href=\"ncbilink:goa/Q9WUU8\">GOA:Q9WUU8</a>" 
COMMENT                                        1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /db_xref=<a href=\"ncbilink:uniprotkb/swiss-prot/Q9WUU8\">UniProtKB/Swiss-Prot:Q9WUU8</a>" 
COMMENT                                        1))) "mABIN-1") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 9534 (3 #26# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 9534  End: 10394 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7423..8283)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (5 total)") 
COMMENT                     #82=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #83=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5957 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5957  End: 5983" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5957..5983" 1))) "HA epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #87=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5075 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5075  End: 5478" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5075..5478" 1))) "ADH1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #92=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8416  End: 8499 (Complementary)" 
COMMENT                                        1))) "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #95=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 2     End: 982  (Complementary)" 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2..982)" 1))) "2mu ori") 
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COMMENT                                          "Start: 2661  End: 3116 (Complementary)" 
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COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(2661..3116)" 1))) 
COMMENT                                  "f1 ori") 
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COMMENT                                          "Start: 8591  End: 8993 (Complementary)" 
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COMMENT                                    #107=(CLinePar 
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COMMENT                                          "   complement(6480..6882)" 1))) 
COMMENT                                  "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #108=(CFolderPar 
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COMMENT                                           "Start: 1060  End: 1164 (Complementary)" 
COMMENT                                           1))) "FLP terminator") 
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COMMENT                                           "Start: 3312  End: 3586 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #113=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(3312..3586)" 1))) 
COMMENT                                   "CYC1 terminator") 
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COMMENT                                    (CObjectList 
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COMMENT                                     #117=(CLinePar 
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COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   6051..6152" 1))) 
COMMENT                                   "ADH1 terminator"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #119=(CFolderPar 
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COMMENT                     (CObjectList 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList)) "Bsp119I: 0 sites") 2 4 "TTCGAA" 
COMMENT                            "AAGCTT") 
COMMENT                      #121=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" 
COMMENT                            "CGCCGGCG") 
COMMENT                      #122=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5998 " 1))))) "NdeI: 1 site") 
COMMENT                            2 4 "CATATG" "GTATAC") 
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COMMENT                                     (CObjectList 
COMMENT                                      #127=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5982 " 1))))) "NheI: 1 site") 
COMMENT                            1 5 "GCTAGC" "CGATCG") 
COMMENT                      #128=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #130=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 8127 " 1))))) "SmaI: 1 site") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
COMMENT                      #131=(CRSFolderPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #133=(CLinePar 
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COMMENT                                            " N1: 4302 (on complementary strand)" 
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FEATURES             Location/Qualifiers
     terminator      complement(1060..1164)
                     /vntifkey="43"
                     /label=FLP\terminator
     terminator      complement(3312..3586)
                     /vntifkey="43"
                     /label=CYC1\terminator
     rep_origin      complement(2..982)
                     /vntifkey="33"
                     /label=2mu\ori
     CDS             1884..2558
                     /vntifkey="4"
                     /label=TRP1
     rep_origin      complement(2661..3116)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        5075..5478
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             5501..5941
                     /vntifkey="4"
                     /label=GALbd
     misc_marker     5957..5983
                     /vntifkey="22"
                     /label=HA\epitope
     CDS             6008..7951
                     /gene="ABINl"
                     /codon_start=1
                     /product="ABINl, A20-binding inhibitor of NF-kappa B
                     activation (large)"
                     /protein_id="CAB44240.1"
                     /db_xref="GI:4995753"
                     /db_xref="GOA:Q9WUU8"
                     /db_xref="UniProtKB/Swiss-Prot:Q9WUU8"
                     /vntifkey="4"
                     /label=mABIN-1
     promoter        complement(8416..8499)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      8162..8263
                     /vntifkey="43"
                     /label=ADH1\terminator
     rep_origin      complement(8591..8993)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(9534..10394)
                     /vntifkey="4"
                     /label=ampicillin
     CDS             complement(3855..4304)
                     /vntifkey="4"
                     /label=CYH2
BASE COUNT     2917 a      2358 c      2498 g      2751 t 
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 
     5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 
     5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 
     6001 ggccgccatg gaagggagag gaccctaccg gatctacgac ccagggggca gcacgcctct 
     6061 gggagaggtg tccgcagctt ttgaacgtct agtggaggag aatactcggc tgaagggaaa 
     6121 aatgcaaggg ataaagatgt taggggagct tctggaggag tctcagatgg aagcgtccag 
     6181 actccggcag aaggcagagg agctggtcaa ggacagcgag ctgtcaccac cgacatctgc 
     6241 cccctccttg gtctcctttg atgacctggc tgagctcaca ggacaggata caaaggtcca 
     6301 ggtacatcct gctaccagca ctgccgccac caccaccgcc accgccacca cgggaaactc 
     6361 catggagaag cccgagccag cctccaaatc tccgtccaat ggcgcctcct cggactttga 
     6421 agtggtccct actgaggagc agaattcacc cgaaactggc agccacccta cgaacatgat 
     6481 ggacctgggg cccccacccc cagaggacag caacctgaag ctccacctgc agcgcctgga 
     6541 gaccaccctt agcgtgtgtg cagaggagcc agaccacagc cagctcttca cccacctggg 
     6601 ccgcatggcc ctcgagttca acaggttggc ctccaaagtg cataaaaatg agcagcgcac 
     6661 ctccatcctg cagaccttat gtgagcagct gcgccaggag aatgaagccc tgaaggccaa 
     6721 gctggacaag ggcctggaac agcgggatct ggctgctgag aggctgcggg aggaaaacac 
     6781 ggagctcaag aaactgttga tgaacagcag ctgcaaagag ggactctgtg ggcagcccag 
     6841 ctccccaaag ccagagggtg ctggcaagaa gggcgtggct ggacagcagc aggccagtgt 
     6901 gatggcgagt aaagtccctg aagcgggggc ctttggagca gctgagaaga aagtgaagtt 
     6961 gctagaacag caacgcatgg agctgctgga agtgaacaag cagtgggacc agcatttccg 
     7021 gtccatgaag cagcagtatg agcagaagat cacagagctt cgccagaagc tggtggacct 
     7081 gcagaaacag gtaactgagc tggaggccga acgggagcag aagcagcgtg actttgaccg 
     7141 gaaactcctc ctggccaaat cgaagataga gatggaagag accgacaagg agcagctgac 
     7201 agcagaggcc aaggaactgc gccagaaggt caggtaccta caggatcagc tgagcccgct 
     7261 cacaaggcaa cgagaatacc aggagaagga gatccagcgg ctcaataagg ccctggagga 
     7321 ggccctcagc atccaggcct ctccatcatc tccgcctgca gcttttggga gtccagaagg 
     7381 cgttgggggc catctgagga agcaggaact agtgacacag aatgagttgc tgaaacagca 
     7441 ggtaaagatc tttgaagagg acttccagag ggaacggagt gaccgtgaac gcatgaatga 
     7501 agagaaggag gagctgaaga agcaagtaga gaagctgcag gcccaggtca ccctgactaa 
     7561 tgcccagctc aaaactctca aagaggagga gaaggccaag gaagccctca aacagcagaa 
     7621 gaggaaagca aaggcttcgg gagagcgcta ccacatggaa ccccaccctg agcacgtctg 
     7681 cggcgcctat ccctatgcct acccacccat gccagccatg gtacctcacc atgcctacaa 
     7741 ggactggtcc cagatccgat accctccacc ccctgtgccc atggagcacc cgcccccaca 
     7801 ccccaactct cgcctcttcc atctgccgga gtacacctgg cgtccaccct gtgcagggat 
     7861 tcggaatcag agctctcaag tgatggaccc gcccccagac aggcctgcag agccagagtc 
     7921 tgcagacaat gactgtgatg ggccccagtg aggctgcagt gggtcatttg gttccacctt 
     7981 catctttcag agccagctga cctcagattg ccaaaagttt gaaggccatg tgcatgttct 
     8041 gtgtgaccca agccttggca gaggagaggc tgggatgggt agctggctca catccccagc 
     8101 caagcctcga actgttgaca agacccgggg atccgtcgac ctgcagccaa gctaattccg 
     8161 ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt 
     8221 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttgttct tgagtaactc 
     8281 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac 
     8341 ctctaccggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 
     8401 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 
     8461 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 
     8521 ggaaacctgt cgtgccagct ggattaatga atcggccaac gcgcggggag aggcggtttg 
     8581 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     8641 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     8701 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     8761 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     8821 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     8881 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     8941 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
     9001 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     9061 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     9121 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     9181 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 
     9241 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     9301 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     9361 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     9421 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     9481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
     9541 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
     9601 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
     9661 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     9721 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     9781 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     9841 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     9901 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     9961 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
    10021 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
    10081 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
    10141 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
    10201 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
    10261 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
    10321 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
    10381 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
    10441 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 
    10501 acatttcccc gaaaagtgcc acct 
//
