LOCUS       pCAGGS-E-mABIN-2(50-200)        5314 bp    DNA     circular      6-JUL-2007
DEFINITION  LMBP 5442.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5442
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 06-JUL-2007
            
COMMENT     VNTDATE|450463772|
COMMENT     VNTNAME|pCAGGS-E-mABIN-2(50-200)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3030 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 519 0 5313 0 0 "")) 
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COMMENT        #6=(CFSignal (CObList) "\337-globin polyA" 43 0 0 599 690 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 691 1047 1 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 1125 1207 1 (CStringList) 
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COMMENT                          "Created: 06/29/07 04:49" 1) 
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COMMENT                          "Last Modified: 07/06/07 03:37" 1) 
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FEATURES             Location/Qualifiers
     misc_marker     11..49
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     misc_feature    691..1047
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      complement(1455..1529)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      1455..1529
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(1644..2045)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      599..690
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     promoter        complement(1125..1207)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="Escherichia coli lac operon promoter"
     rep_origin      1349..1424
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     intron          4334..5258
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="intron of the chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3965..5306
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3582..3959
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early promoter; enhancer only"
     CDS             complement(2586..3446)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     CDS             59..511
                     /vntifkey="4"
                     /label=mABIN-2(50-200)
                     /note="Mouse A20-binding inhibitor of NF-kappaB activation 2 cDNA; fragment encoding aa 50-200"
BASE COUNT     1126 a      1409 c      1510 g      1269 t 
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgccac 
       61 ccgcctcgcg gctctggaag ggcacacggc gccgtcactc gtggacgcac ttctggatca 
      121 ggtggagcgc ttccgtgagc agctgcgacg acaggaggaa ggcgcttcgg agacccagct 
      181 gcggcaggaa gttgaaagac ttacggagcg tctagaggaa aaagagaggg agatgcaaca 
      241 gctgatgagc cagcctcagc atgagcaaga gaaggaggta gtcttgcttc ggcgaagtgt 
      301 ggcagagaag gagaaagcca gggccgccag tgatgttctg tgccgctcct tggctgatga 
      361 gacccaccaa ctgcgcagga cattggcagc cactgcccac atgtgccaac atctggccaa 
      421 atgtctggat gaacgacagt gtgcacaggg agacgctggg gagaaaagcc ctgctgagct 
      481 agagcaaaca agcagcgatg cttctggctg actcgaggaa ttcactcctc aggtgcaggc 
      541 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga 
      601 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc 
      661 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc 
      721 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta 
      781 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg 
      841 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac 
      901 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa 
      961 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat 
     1021 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat 
     1081 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 
     1141 ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg agctaactca cattaattgc 
     1201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc 
     1261 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 
     1321 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 
     1381 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 
     1441 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     1501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     1561 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg 
     1621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     1681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     1741 agaatcaggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
     1801 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
     1861 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
     1921 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
     1981 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 
     2041 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
     2101 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
     2161 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
     2221 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 
     2281 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
     2341 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
     2401 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
     2461 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 
     2521 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
     2581 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 
     2641 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 
     2701 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 
     2761 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 
     2821 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
     2881 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 
     2941 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 
     3001 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 
     3061 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 
     3121 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
     3181 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 
     3241 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 
     3301 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 
     3361 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 
     3421 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
     3481 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
     3541 ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat tattgactag 
     3601 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt 
     3661 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac 
     3721 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg 
     3781 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag 
     3841 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat 
     3901 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat 
     3961 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc 
     4021 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg 
     4081 ggggcccccc ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggaaagg 
     4141 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg 
     4201 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgttg cgcgctgcct 
     4261 tccccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc 
     4321 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt 
     4381 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga 
     4441 gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag 
     4501 cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct 
     4561 ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg 
     4621 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg 
     4681 gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga 
     4741 gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc 
     4801 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga 
     4861 gggctcgggg gaaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga 
     4921 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc 
     4981 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg 
     5041 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc 
     5101 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc 
     5161 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct 
     5221 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta 
     5281 ttgtgctgtc tcatcatttt ggcaaagaat tgaa 
//
