ID pAS2-mABIN-2 standard; circular DNA; ; 9714 BP. DE LMBP 5448. CC Accession number: LMBP 5448 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 18-SEP-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT misc_marker 5957..5983 FT /vntifkey="22" FT /label=HA\epitope FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT CDS 6026..7318 FT /vntifkey="4" FT /label=mABIN-2 FT terminator 7352..7453 FT /vntifkey="43" FT /label=ADH1\terminator FT rep_origin complement(7781..8183) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(8724..9584) FT /vntifkey="4" FT /label=ampicillin FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT promoter complement(7606..7689) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 9714 BP; 2691 A; 2076 C; 2299 G; 2648 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 6000 ggccatggag gccccgggga tccgtatgtc gtctggggac ccaaggtctg gtagacagga 6060 cggggccccg cgtgcggccg cagcgctctg tggcctgtac cacgaggccg gccagcaact 6120 acagcgcctg aaggatcagc tggccgcgcg tgacgccctc atcgcgagcc tccgcacccg 6180 cctcgcggct ctggaagggc acacggcgcc gtcactcgtg gacgcacttc tggatcaggt 6240 ggagcgcttc cgtgagcagc tgcgacgaca ggaggaaggc gcttcggaga cccagctgcg 6300 gcaggaagtt gaaagactta cggagcgtct agaggaaaaa gagagggaga tgcaacagct 6360 gatgagccag cctcagcatg agcaagagaa ggaggtagtc ttgcttcggc gaagtgtggc 6420 agagaaggag aaagccaggg ccgccagtga tgttctgtgc cgctccttgg ctgatgagac 6480 ccaccaactg cgcaggacat tggcagccac tgcccacatg tgccaacatc tggccaaatg 6540 tctggatgaa cgacagtgtg cacagggaga cgctggggag aaaagccctg ctgagctaga 6600 gcaaacaagc agcgatgctt ctggccagag tgttattaag aagttacagg aagaaaatcg 6660 actgttaaaa cagaaggtga ctcatgtaga agacctcaat gctaagtggc agcgttatga 6720 tgcaagtagg gacgaatatg tgaaggggtt gcatgcccag ctaaagaggc ggcaggtccc 6780 tctggagcct gagctgatga agaaggagat ttcccgactt aacagacagt tggaggagaa 6840 aataagtgac tgtgcggaag caaaccagga gctgacagcc atgaggatgt cccgggacac 6900 tgcgctggag cgagtgcaga tgctagaaca gcagattctt gcttacaagg atgacttcaa 6960 atcagaaagg gcagatcggg aacgagcgca cagtaggatt caagagctgg aggaaaagat 7020 catgtccttg atgtaccaag tgtcccagag acaggactcc cgggagccag gaccctgtcg 7080 gattcatacg gggaacaaaa ctgccaagta cttagagatg gatgcactgg agcatgtgac 7140 ccctggcggc tggaggcctg agtctaggtc ccaacagatg gaaccttctg cagagggtgg 7200 gcatgtgtgc acagcccaga gaggtcaggg tgaccttcag tgccctcatt gcctgcggtg 7260 cttcagtgat gagcaaggcg aggcattcct caggcacctg tctgagtgct gccaatgaag 7320 atccgtcgac ctgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat 7380 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt 7440 aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt 7500 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca 7560 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 7620 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt 7680 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct ggattaatga 7740 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 7800 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 7860 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 7920 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 7980 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 8040 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 8100 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 8160 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 8220 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 8280 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 8340 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 8400 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 8460 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 8520 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 8580 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 8640 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 8700 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 8760 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 8820 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 8880 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 8940 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 9000 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 9060 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 9120 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 9180 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 9240 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 9300 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 9360 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 9420 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 9480 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 9540 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 9600 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 9660 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acct 9714 //