LOCUS       pCal-n-AK               7591 bp    DNA     circular     17-JUL-2012
DEFINITION  LMBP 5461.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5461
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-JUL-2012
            
            
COMMENT     VNTDATE|489335830|
COMMENT     VNTNAME|pCal-n-AK|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCal-n-AK" 0 7591 
COMMENT       (CDBMol 0 0 1 5230 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AatII" "Alw44I" "BamHI" "BcuI" "BglII" "HincII" "NcoI" 
COMMENT         "PaeI" "PdmI" "PstI" "SacI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "2mu ori" 
COMMENT             "AGAAAGTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGCACTTTCAAAAAACCAAAAACGCACCGGACTGTAACGAGCTACTAAAATATTGCGAATACCGCTTCCACAAACATTGCTCAAAAGTATCTCTTTGCTATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACTCGACCTCTACATTTTTTATGTTTATCTCTAGTATTACTCTTTAGACAAAAAAATTGTAGTAAGAACTATTCATAGAGTGAATCGAAAACAATACGAAAATGTAAACATTTCCTATACGTAGTATATAGAGACAAAATAGAAGAAACCGTTCATAATTTTCTGACCAATGAAGAATCATCAACGCTATCACTTTCTGTTCACAAAGTATGCGCAATCCACATCGGTATAGAATATAATCGGGGATGCCTTTATCTTGAAAAAATGCACCCGCAGCTTCGCTAGTAATCAGTAAACGCGGGAAGTGGAGTCAGGCTTTTTTTATGGAAGAGAAAATAGACACCAAAGTAGCCTTCTTCTAACCTTAACGGACCTACAGTGCAAAAAGTTATCAAGAGACTGCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTTAGAAAAATAGCGCTCTCGGGATGCATTTTTGTAGAACAAAAAAGAAGTATAGATTCTTTGTTGGTAAAATAGCGCTCTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTTTACAAAAATGAAGCACAGATTCTTCGTTGGTAAAATAGCGCTTTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTCTACAAAATGAAGCACAGATGCTTCGTT" 
COMMENT             "Fragment of pAS3; Length: 981" 0 (CStringList) 0) 
COMMENT         #1=(COligo "3' FR of \337-globin" 
COMMENT             "AATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATATGGGAGGGCAAATCATTTAAAACATCAGAATGAGTATTTGGTTTAGAGTTTGGCAACATATGCCCATATGCTGGCTGCCATGAACAAAGGTTGGCTATAAAGAGGTCATCAGTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTGACTTGAGGTTAGATTTTTTTTATATTTTGTTTTGTGTTATTTTTTTCTTTAACATCCCTAAAATTTTCCTTACATGTTTTACTAGCCAGATTTTTCCTCCTCTCCTGACTACTCCCAGTCATAGCTGTCCCTCTTCTCTTATGGAGATC" 
COMMENT             "Fragment of pCAGGS-FLAGAVPShOMI; Length: 357" 0 (CStringList) 0) 
COMMENT         #2=(COligo "3' MMLV-LTR" 
COMMENT             "AATGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTCAGGAACAGATGGAACAGCTGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGAACAGCTGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCAGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGGGCGCCAGTCCTCCGATTGACTGAGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTTGCAGTTGCATCCGACTTGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGGTCTTTCATT" 
COMMENT             "Fragment of LNXCO3; Length: 594" 0 (CStringList) 0) 
COMMENT         #3=(COligo "3' MSCV-PCMV LTR" 
COMMENT             "CTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTACCCGTGTATCCAATAAACCCTCTTGCAGTTGCATCCGACTTGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCGTCAGCGGGGGTCTTTCA" 
COMMENT             "Fragment of pMSCV-F-BirA; Length: 485" 0 (CStringList) 0) 
COMMENT         #4=(COligo "5' MMLV-LTR" 
COMMENT             "TTTGAAAGACCCCACCCGTAGGTGGCAAGCTAGCTTAAGTAACGCCACTTTGCAAGGCATGGAAAAATACATAACTGAGAATAGAAAAGTTCAGATCAAGGTCAGGAACAAAGAAACAGCTGAATACCAAACAGGATATCTGTGGTAAGCGGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGAGACAGCTGAGTGATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCGGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCAGCCCTCAGCAGTTTCTAGTGAATCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAAATGACCCTGTACCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCCGCTCTCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCTTCCGATAGACTGCGTCGCCCGGGTACCCGTATTCCCAATAAAGCCTCTTGCTGTTTGCATCCGAATCGTGGTCTCGCTGTTCCTTGGGAGGGTCTCCTCTGAGTGATTGACTACCCACGACGGGGGTCTTTCATT" 
COMMENT             "Fragment of LNXCO3; Length: 589" 0 (CStringList) 0) 
COMMENT         #5=(COligo "5' MSCV-PCMV LTR" 
COMMENT             "TGAAAGACCCCACCTGTAGGTTTGGCAAGCTAGCTTAAGTAACGCCATTTTGCAAGGCATGGAAAATACATAACTGAGAATAGAGAAGTTCAGATCAAGGTTAGGAACAGAGAGACAGCAGAATATGGGCCAAACAGGATATCTGTGGTAAGCAGTTCCTGCCCCGGCTCAGGGCCAAGAACAGATGGTCCCCAGATGCGGTCCCGCCCTCAGCAGTTTCTAGAGAACCATCAGATGTTTCCAGGGTGCCCCAAGGACCTGAAAATGACCCTGTGCCTTATTTGAACTAACCAATCAGTTCGCTTCTCGCTTCTGTTCGCGCGCTTCTGCTCCCCGAGCTCAATAAAAGAGCCCACAACCCCTCACTCGGCGCGCCAGTCCTCCGATAGACTGCGTCGCCCGGGTAC" 
COMMENT             "Fragment of pMSCV-F-BirA; Length: 407" 0 (CStringList) 0) 
COMMENT         #6=(COligo "5' UTR of AOX1" 
COMMENT             "ACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAA" 
COMMENT             "Fragment of pPP1MFmds1; Length: 165" 0 (CStringList) 0) 
COMMENT         #7=(COligo "ADE2 (1)" 
COMMENT             "ATGGATTCTAGAACAGTTGGTATATTAGGAGGGGGACAATTGGGACGTATGATTGTTGAGGCAGCAAACAGGCTCAACATTAAGACGGTAATACTAGATGCTGAAAATTCTCCTGCCAAACAAATAAGCAACTCCAATGACCACGTTAATGGCTCCTTTTCCAATCCTCTTGATATCGAAAAACTAGCTGAAAAATGTGATGTGCTAACGATTGAGATTGAGCATGTTGATGTTCCTACACTAAAGAATCTTCAAGTAAAACATCCCAAATTAAAAATTTACCCTTCTCCAGAAACAATCAGATTGATACAAGACAAATATATTCAAAAAGAGCATTTAATCAAAAATGGTATAGCAGTTACCCAAAGTGTTCCTGTGGAACAAGCCAGTGAGACGTCCCTATTGAATGTTGGAAGAGATTTGGGTTTTCCATTCGTCTTGAAGTCGAGGACTTTGGCATACGATGGAAGAGGTAACTTCGTTGTAAAGAATAAGGAAATGATTCCGGAAGCTTTGGAAGTACTGAAGGATCGTCCTTTGTACGCCGAAAAATGGGCACCATTTACTAAAGAATTAGCAGTCATGATTGTGAGATCTGTTAACGGTTTAGTGTTTTCTTACCCAATTGTAGAGACTATCCACAAGGACAATATTTGTGACTTATGTTATGCGCCTGCTAGAGTTCCGGACTCCGTTCAACTTAAGGCGAAGTTGTTGGCAGAAAATGCAATCAAATCTTTTCCCGGTTGTGGTATATTTGGTGTGGAAATGTTCTATTTAGAAACAGGGGAATTGCTTATTAACGAAATTGCCCCAAGGCCTCACAACTCTGGACATTATACCATTGATGCTTGCGTCACTTCTCAATTTGAAGCTCATTTGAGATCAATATTGGATTTGCCAATGCCAAAGAATTTCACATCTTTCTCCACCATTACAACGAACGCCATTATGCTAAATGTTCTTGGAGACAAACATACAAAAGATAAAGAGCTAGAAA" 
COMMENT             "Fragment of pAS3; Length: 1000" 0 (CStringList) 0) 
COMMENT         #8=(COligo "ADE2 (2)" 
COMMENT             "CTTGCGAAAGAGCATTGGCGACTCCAGGTTCCTCAGTGTACTTATATGGAAAAGAGTCTAGACCTAACAGAAAAGTAGGTCACATAAATATTATTGCCTCCAGTATGGCGGAATGTGAACAAAGGCTGAACTACATTACAGGTAGAACTGATATTCCAATCAAAATCTCTGTCGCTCAAAAGTTGGACTTGGAAGCAATGGTCAAACCATTGGTTGGAATCATCATGGGATCAGACTCTGACTTGCCGGTAATGTCTGCCGCATGTGCGGTTTTAAAAGATTTTGGCGTTCCATTTGAAGTGACAATAGTCTCTGCTCATAGAACTCCACATAGGATGTCAGCATATGCTATTTCCGCAAGCAAGCGTGGAATTAAAACAATTATCGCTGGAGCTGGTGGGGCTGCTCACTTGCCAGGTATGGTGGCTGCAATGACACCACTTCCTGTCATCGGTGTGCCCGTAAAAGGTTCTTGTCTAGATGGAGTAGATTCTTTACATTCAATTGTGCAAATGCCTAGAGGTGTTCCAGTAGCTACCGTCGCTATTAATAATAGTACGAACGCTGCGCTGTTGGCTGTCAGACTGCTTGGCGCTTATGATTCAAGTTATACAACGAAAATGGAACAGTTTTTATTAAAGCAAGAAGAAGAAGTTCTTGTCAAAGCACAAAAGTTAGAAACTGTCGGTTACGAAGCTTATCTAGAAAACAAGTAA" 
COMMENT             "Fragment of pAS3; Length: 716" 0 (CStringList) 0) 
COMMENT         #9=(COligo "ADH1 promoter" 
COMMENT             "TGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAGTTTGCCGCTTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTCCAAG" 
COMMENT             "Fragment of pAS3; Length: 404" 0 (CStringList) 0) 
COMMENT         #10=(COligo "ADH1 terminator" 
COMMENT              "GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTC" 
COMMENT              "Fragment of pRH007; Length: 102" 0 (CStringList) 0) 
COMMENT         #11=(COligo "AG promoter (1)" 
COMMENT              "GAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGGCCCCCCCCAGGCGGGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGAAAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGTTGCGCGCTGCCTTCCCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGGGCTCGGGGGAAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCAG" 
COMMENT              "Fragment of pCAGGS-FLAGAVPShOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #12=(COligo "AG promoter (2)" 
COMMENT              "GGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAA" 
COMMENT              "Fragment of pCAGGS-FLAGAVPShOMI; Length: 342" 0 (CStringList) 0) 
COMMENT         #13=(COligo "amp promoter" 
COMMENT              "CAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCT" 
COMMENT              "Fragment of pdEYFP; Length: 96" 0 (CStringList) 0) 
COMMENT         #14=(COligo "AMP RBS" "GATAAATGCTTCAATAATATTGAAAAAGGAAGAGT" 
COMMENT              "Fragment of pdECFP; Length: 35" 0 (CStringList) 0) 
COMMENT         #15=(COligo "ampicillin" 
COMMENT              "ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA" 
COMMENT              "Fragment of pEF6-E-mTRAF2; Length: 861" 0 (CStringList) 0) 
COMMENT         #16=(COligo "AOX1 promoter" 
COMMENT              "AACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGCCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTG" 
COMMENT              "Fragment of pPP1MFmds1; Length: 766" 0 (CStringList) 0) 
COMMENT         #17=(COligo "AOX1 terminator" 
COMMENT              "TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGAAGTTCGTTTGTGC" 
COMMENT              "Fragment of pPP1MFmds1; Length: 260" 0 (CStringList) 0) 
COMMENT         #18=(COligo "attR1" 
COMMENT              "CATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTTTTGTACAAACTTGT" 
COMMENT              "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #19=(COligo "attR2" 
COMMENT              "CATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGT" 
COMMENT              "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #20=(COligo "birA" 
COMMENT              "ATGAAGGATAACACCGTGCCACTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCAGTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTCAGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGATACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGGTCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGAATCAGTACCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGCATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTTTTCGCCTTTTGGCGCAAACTTATATTTGTCGATGTTCTGGCGTCTGGAACAAGGCCCGGCGGCGGCGATTGGTTTAAGTCTGGTTATCGGTATCGTGATGGCGGAAGTATTACGCAAGCTGGGTGCAGATAAAGTTCGTGTTAAATGGCCTAATGACCTCTATCTGCAGGATCGCAAGCTGGCAGGCATTCTGGTGGAGCTGACTGGCAAAACTGGCGATGCGGCGCAAATAGTCATTGGAGCCGGGATCAACATGGCAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAA" 
COMMENT              "Fragment of MSCVp-F-BirA; Length: 966" 0 (CStringList) 0) 
COMMENT         #21=(COligo "BK ori" "GAGGCCGAGGCCGCCTCTGCCTC" 
COMMENT              "Fragment of pBK-1; Length: 23 " 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #22=(COligo "blasticidin" 
COMMENT              "ATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAA" 
COMMENT              "Fragment of pEF6-E-mTRAF2; Length: 399" 0 (CStringList) 0) 
COMMENT         #23=(COligo "bleomycin" 
COMMENT              "ATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACGGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCAAGCTGGAGTTCTTCGCCCACCCCGGGCTCGATCCCCTCGCGAGTTGGTTCAGCTGCTGCCTGAGGCTGGACGACCTCGCGGAGTTCTACCGGCAGTGCAAATCCGTCGGCATCCAGGAAACCAGCAGCGGCTATCCGCGCATCCATGCCCCCGAACTGCAGGAGTGGGGAGGCACGATGGCCGCTTTGGTCGACCCGGACGGGACGCTCCTGCGCCTGATACAGAACGAATTGCTTGCAGGCATCTCATGA" 
COMMENT              "Fragment of pSCUDMFE6L2; Length: 381" 0 (CStringList) 0) 
COMMENT         #24=(COligo "bovine growth hormone polyA" 
COMMENT              "CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG" 
COMMENT              "Fragment of pEF1hCASP-9S-His; Length: 225" 0 (CStringList) 0) 
COMMENT         #25=(COligo "CaGAL1 promoter (1)" 
COMMENT              "AGATCTGATATTGACGAAGAGGAACCAATGTAACGTTACACTGAAGAAAACACACAATAAACGGGAAGAAACGGTGTAAAAGTGTGAAAATAATTTTTGAATATCATTTCCCTTGGTTTAATTCCAAACGAAACGTGTTTTTTTTAGAGAATGGGAATTCTTATTGGATGTCTAGATTGTTTGTTTACTCCAGACTGTGCACAAAAACGTTTGGATGGATGATCAGAAGATATTTTTAGGCTTAGCTCTAAATATAAGAAATGATGCTTGAAAAACCAGACAGAAATTGAGTTTCAAAAATTGGTAATGTGAGGTATTAGTCAACTAACCAAATAACAATGCAAACCGGTTGATACATTTCATTTTGAAAATAATGAAACTGGAATTGGATGACCAGCACACAAACACATAAAGTAATTATGGGAATTAGAAGCGAACATAGAGGAGTACTTGGCCACGAACAGAATACAAGTGGGAACACTATTTTCTCCATTGTTTTAGTTCTGTTTTTTTGTCAGCCTAGTTTTGTGCTATGTGTAAAAAATATTGCCAAGAAAAAAAGCTTGTTTTGTGGCCAGTGTCCGAAAAAAATTTTGGGGAATCTTCGGATTAATTTATGTTTTCATTCCATCGGGGAAAGTGGGGGGGAAAAAATTTTAAGCAGTTCACAAAACCTTCCAAAAAATATATGGACAAAGATGATTGTATTTTCCCGACACCAAAATCATAATTAATTATGAGAAAGTTAAATGTAACGTTACAATTTATGTTTATTTGAAGGTGAAAAGCGATTTATGATTTTTCCGAAATGAAAATTTTTTTTAGGTTTATTTTTTTTGTCGGGCAAAGAAAAACTGAACAAGGATTATTAAAATTTTTGGTGTTTGTTTGTGTCTGGAGAATTCATTCCTCTCTCATCTTCACACAATGTTTAGACATCTGACACGATTCATGATAGTTCGGTTTCCGGGGTTGGTGTTTAGTTTTCGTTTTTCTTTTT" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 1000" 0 (CStringList) 0) 
COMMENT         #26=(COligo "CaGAL1 promoter (2)" 
COMMENT              "TTTTGGAAAGAATGTTTTAGCTCATTGGTTTTCTTTCTTCATTCAATAGTTTTGAAAGAATTTGCCCACTTGTTATTACAATCATATAAAATTAAACTTTGATATAAAATAGAGTTTGAAAGTTTCCCAGATCCTTTTTGATTTCTTTGTAAATTTTTTTTTCTCCCACATATACACACATACAAACCGATTTTTATAAGAAAGAGTTATACC" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 213" 0 (CStringList) 0) 
COMMENT         #27=(COligo "calmodulin-binding peptide" 
COMMENT              "AAGCGACGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGCCAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTT" 
COMMENT              "Fragment of pCal-c-A7M8K; Length: 78" 0 (CStringList) 0) 
COMMENT         #28=(COligo "CaMV promoter" 
COMMENT              "GATCTCCTTTGCCCCGGAGATCACCATGGACGACTTTCTCTATCTCTACGATCTAGGAAGAAAGTTCGACGGAGAAGGTGACGATACCATGTTCACCACCGATAATGAGAAGATTAGCCTCTTCAATTTCAGAAAGAATGCTGACCCACAGATGGTTAGAGAGGCCTACGCGGCAGGTCTCATCAAGACGATCTACCCGAGTAATAATCTCCAGGAGATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCATAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAGAAAGTAGTTCCTACTGAATCAAAGGCCATGGAGTCAAAAATTCAGATCGAGGATCTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCT" 
COMMENT              "Fragment of pEX1SOD; Length: 947" 0 (CStringList) 0) 
COMMENT         #29=(COligo "CaURA3" 
COMMENT              "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #30=(COligo "ccdB" 
COMMENT              "ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA" 
COMMENT              "Fragment of pRH001; Length: 306" 0 (CStringList) 0) 
COMMENT         #31=(COligo "CEN6/ARSH4 ori" 
COMMENT              "CTTTTCATCACGTGCTATAAAAATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAACAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGTGTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTTATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGGCAAGCGATCCGTCCTAAGAAACCA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 525" 0 (CStringList) 0) 
COMMENT         #32=(COligo "chloramphenicol" 
COMMENT              "ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA" 
COMMENT              "Fragment of pRH001; Length: 660" 0 (CStringList) 0) 
COMMENT         #33=(COligo "ColE1 ori" 
COMMENT              "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCTATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCTGCTACGTGGCCTTCTTCCTGTTCCTGGTCTTTTGCTCACATGTTCTTTCCGGCCTTATCCCCTGATTCTGTGGATAACTGTGTTACCGTTTTTGTGTGAGTCAGTACCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAAAGC" 
COMMENT              "Fragment of pPLa2311trpA1; Length: 392" 0 (CStringList) 0) 
COMMENT         #34=(COligo "CYC1 promoter" 
COMMENT              "GACAATCTTACATGGTCTACCTTTGATGACAACGAAACCATTCTTTCTCAAGGCAGAACATTGCATTGGGTAGGTGGCGGAGGCACCAGCGTCAGCATTTTCAAAGGTTGTGTTCTTCGTCAGACATGTTTTAGTGTGTGAATGAAATAGGTGTATGTTTTCTTTTGCAGACAATAATTAGGAACAAGGTAAGGGAACTAAAGTGTAGAATAAGAATTAAAAAAGAAGAACAAGTTGAAAGGCAAGTTGAAATTTCAAGAAAAAAGTCAATTGAAGTACAGTAAATTGACCTGAATATATCTGAGTTCCGAGCAACAATGAGTTTACCGAAGAGAACAATGGAATAGGAAACTTTGAACGAAGAAAGGAAAGCAGGAAAGGAAAAAATTTTTAGGCTCGAGAACAATAGGGCAAAAAAACAGGCAACGAACGAACAAATGGAAAAACGAAAAAAAAAAAAACACAGAAAAGAATGCAGAAAGTTGTAAACTGAAAAAAAAAAAAAAAAAAGGTGAACACAGGAAAAAAAATAAAAAAAAAAAAAAAGGAGGACGAAACAAAAAAGTGAAAAAAAATGAAAATTTTTTTGGAAAACCAAGAAATGAATTATATTTCCGTGTGAGACGACATCGTCGAATATGATTCAGGGTAACAGTATTTATGTAATCAATTTCCTACCTGAATCTAAAATTCCCGGGAGCAAGATCAAGATGTTTTCACCGATCTTTCCGGTCTCTTTGGCCGGGGTTTACGGACGATGACCGAAGACCAAGCGCCAGCTCATTTGGCGAGCGTTGGTTGGTGGATCAAGCCCACGCGTAGGCAATCCTCGAGCAGATCCGCCAGGCGTGTATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCT" 
COMMENT              "Fragment of pEX2; Length: 982" 0 (CStringList) 0) 
COMMENT         #35=(COligo "CYC1 terminator" 
COMMENT              "ACAGGCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAG" 
COMMENT              "Fragment of pAS2; Length: 276  (laatste N: G (276) ipv A (275) -> midden HindIII)" 
COMMENT              0 (CStringList) 0) 
COMMENT         #36=(COligo "CYH2" 
COMMENT              "ATGCCTTCCAGATTCACTAAGACTAGAAAGCACAGAGGTCACGTCTCAGCCGGTAAAGGTCGTATCGGTAAGCACAGAAAGCACCCCGGTGGTAGAGGTATGGCCGGTGGTCAACATCACCACAGAATTAACATGGATAAATACCATCCAGGTTATTTCGGTAAGGTTGGTATGAGATACTTCCACAAGCAACAAGCTCATTTCTGGAAGCCAGTCTTGAACTTGGACAAATTGTGGACATTGATCCCAGAAGACAAGAGAGACCAATACTTGAAATCTGCTTCTAAGGAAACTGCTCCAGTTATTGACACTTTGGCAGCCGGTTACGGTAAGATCTTGGGTAAGGGTAGAATTCCAAATGTTCCAGTTATCGTCAAAGCTAGATTCGTCTCCAAGTTGGCTGAAGAAAAAATCAGAGCTGCTGGTGGTGTTGTTGAATTGATCGCTTAA" 
COMMENT              "Fragment of pAS2; Length: 450" 0 (CStringList) 0) 
COMMENT         #37=(COligo "E-tag" "GGNGCNCCNGTNCCNTAYCCNGAYCCNYTNGARCCNCGN" 
COMMENT              " Fragment of pYD1TSE ; Length: 39" 0 (CStringList) 0) 
COMMENT         #38=(COligo "EBNA1 (1)" 
COMMENT              "ATGTCTGACGAGGGGCCAGGTACAGGACCTGGAAATGGCCTAGGAGAGAAGGGAGACACATCTGGACCAGAAGGCTCCGGCGGCAGTGGACCTCAAAGAAGAGGGGGTGATAACCATGGACGAGGACGGGGAAGAGGACGAGGACGAGGAGGCGGAAGACCAGGAGCCCCGGGCGGCTCAGGATCAGGGCCAAGACATAGAGATGGTGTCCGGAGACCCCAAAAACGTCCAAGTTGCATTGGCTGCAAAGGGACCCACGGTGGAACAGGAGCAGGAGCAGGAGCGGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGGGCAGGAGGGGCAGGAGCAGGAGGAGGGGCAGGAGCAGGAGGGGCAGGAGCAGGAGGTGGAGGCCGGGGTCGAGGAGGCA" 
COMMENT              "Fragment of pREP4FLAG; Length: 1000" 0 (CStringList) 0) 
COMMENT         #39=(COligo "EBNA1 (2)" 
COMMENT              "GTGGAGGCCGGGGTCGAGGAGGTAGTGGAGGCCGGGGTCGAGGAGGTAGTGGAGGCCGCCGGGGTAGAGGACGTGAAAGAGCCAGGGGGGGAAGTCGTGAAAGAGCCAGGGGGAGAGGTCGTGGACGTGGAGAAAAGAGGCCCAGGAGTCCCAGTAGTCAGTCATCATCATCCGGGTCTCCACCGCGCAGGCCCCCTCCAGGTAGAAGGCCATTTTTCCACCCTGTAGGGGAAGCCGATTATTTTGAATACCACCAAGAAGGTGGCCCAGATGGTGAGCCTGACGTGCCCCCGGGAGCGATAGAGCAGGGCCCCGCAGATGACCCAGGAGAAGGCCCAAGCACTGGACCCCGGGGTCAGGGTGATGGAGGCAGGCGCAAAAAAGGAGGGTGGTTTGGAAAGCATCGTGGTCAAGGAGGTTCCAACCCGAAATTTGAGAACATTGCAGAAGGTTTAAGAGCTCTCCTGGCTAGGAGTCACGTAGAAAGGACTACCGACGAAGGAACTTGGGTCGCCGGTGTGTTCGTATATGGAGGTAGTAAGACCTCCCTTTACAACCTAAGGCGAGGAACTGCCCTTGCTATTCCACAATGTCGTCTTACACCATTGAGTCGTCTCCCCTTTGGAATGGCCCCTGGACCCGGCCCACAACCTGGCCCGCTAAGGGAGTCCATTGTCTGTTATTTCATGGTCTTTTTACAAACTCATATATTTGCTGAGGTTTTGAAGGATGCGATTAAGGACCTTGTTATGACAAAGCCCGCTCCTACCTGCAATATCAGGGTGACTGTGTGCAGCTTTGACGATGGAGTAGATTTGCCTCCCTGGTTTCCACCTATGGTGGAAGGGGCTGCCGCGGAGGGTGATGACGGAGATGACGGAGATGAAGGAGGTGATGGAGATGAGGGTGAGGAAGGGCAGGAGTGA" 
COMMENT              "Fragment of pREP4FLAG; Length: 926" 0 (CStringList) 0) 
COMMENT         #40=(COligo "ECFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACATCAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pECFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #41=(COligo "EGFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEGFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #42=(COligo "EK recognition site" "GAYGAYGAYGAYAAR" "Length: 15 bp" 0 
COMMENT              (CStringList) 0) 
COMMENT         #43=(COligo "Epstein-Barr oriP (1)" 
COMMENT              "CAGGAAAAGGACAAGCAGCGAAAATTCACGCCCCCTTGGGAGGTGGCGGCATATGCAAAGGATAGCACTCCCACTCTACTACTGGGTATCATATGCTGACTGTATATGCATGAGGATAGCATATGCTACCCGGATACAGATTAGGATAGCATATACTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCCTATGCTACCCAGATATAAATTAGGATAGCATATACTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCCTATGCTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCATATGCTATCCAGATATTTGGGTAGTATATGCTACCCAGATATAAATTAGGATAGCATATACTACCCTAATCTCTATTAGGATAGCATATGCTACCCGGATACAGATTAGGATAGCATATACTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCCTATGCTACCCAGATATAAATTAGGATAGCATATACTACCCAGATATAGATTAGGATAGCATATGCTACCCAGATATAGATTAGGATAGCCTATGCTACCCAGATATAGATTAGGATAGCATATGCTATCCAGATATTTGGGTAGTATATGCTACCCATGGCAACATTAGCCCACCGTGCTCTCAGCGACCTCGTGAATATGAGGACCAACAACCCTGTGCTTGGCGCTCAGGCGCAAGTGTGTGTAATTTGTCCTCCAGATCGCAGCAATCGCGCCCCTATCTTGGCCCGCCCACCTACTTATGCAGGTATTCCCCGGGGTGCCATTAGTGGTTTTGTGGGCAAGTGGTTTGACCGCAGTGGTTAGCGGGGTTACAATCAGCCAAGTTATTACACCCTTATTTTACAGTCCAAAACCGCAGGGCGGCGTGTGGGGGCTGACGCGTGCCCCCACTCCACAATTT" 
COMMENT              "Fragment of pREP4FLAG; Length: 1000" 0 (CStringList) 0) 
COMMENT         #44=(COligo "Epstein-Barr oriP (2)" 
COMMENT              "CAAAAAAAAGAGTGGCCACTTGTCTTTGTTTATGGGCCCCATTGGCGTGGAGCCCCGTTTAATTTTCGGGGGTGTTAGAGACAACCAGTGGAGTCCGCTGCTGTCGGCGTCCACTCTCTTTCCCCTTGTTACAAATAGAGTGTAACAACATGGTTCACCTGTCTTGGTCCCTGCCTGGGACACATCTTAATAACCCCAGTATCATATTGCACTAGGATTATGTGTTGCCCATAGCCATAAATTCGTGTGAGATGGACATCCAGTCTTTACGGCTTGTCCCCACCCCATGGATTTCTATTGTTAAAGATATTCAGAATGTTTCATTCCTACACTAGTATTTATTGCCCAAGGGGTTTGTGAGGGTTATATTGGTGTCATAGCACAATGCCACCACTGAACCCCCCGTCCAAATTTTATTCTGGGGGCGTCACCTGAAACCTTGTTTTCGAGCACCTCACATACACCTTACTGTTCACAACTCAGCAGTTATTCTATTAGCTAAACGAAGGAGAATGAAGAAGCAGGCGAAGATTCAGGAGAGTTCACTGCCCGCTCCTTGATCTTCAGCCACTGCCCTTGTGACTAAAATGGTTCACTACCCTCGTGGAATCCTGACCCCATGTAAATAAAACCGTGACAGCTCATGGGGTGGGAGATATCGCTGTTCCTTAGGACCCTTTTACTAACCCTAATTCGATAGCATATGCTTCCCGTTGGGTAACATATGCTATTGAATTAGGGTTAGTCTGGATAGTATATACTACTACCCGGG" 
COMMENT              "Fragment of pREP4FLAG; Length: 772" 0 (CStringList) 0) 
COMMENT         #45=(COligo "EYFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEYFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #46=(COligo "f1 ori" 
COMMENT              "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT              "Fragment of pAS2; Length: 456" 0 (CStringList) 0) 
COMMENT         #47=(COligo "fd terminator" 
COMMENT              "TAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGAGATTTTCAACGTGAAAAAATTATTATTCGCAATTCC" 
COMMENT              "Fragment of bacteriophage_fd; Length: 82, zoals in pMa58" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #48=(COligo "FLAG epitope" "GAYTAYAARGAYGAYGAYGAYAAR" 
COMMENT              " Fragment of pCAGGS-FLAGmA20; Length: 24" 0 (CStringList) 0) 
COMMENT         #49=(COligo "FLD1 promoter start" 
COMMENT              "GCATGCAGGAATCTCTGGCACGGTGCTAATGGTAGTTATCCAACGGAGCTGAGGTAGTCGATATATCTGGATATGCCGCCTATAGGATAAAAACAGGAGA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #50=(COligo "FLD1 promoter stop" 
COMMENT              "TTTAGAGCCTTACCTGATCGCAATCAGGATTTCACTACTCATATAAATACATCGCTCAAAGCTCCAACTTTGCTTGTTCATACAATTCTTGATATTCACA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #51=(COligo "FLP terminator" 
COMMENT              "GTACGCATTTAAGCATAAACACGCACTATGCCGTTCTTCTCATGTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTGTATGTGC" 
COMMENT              "Fragment of pSCGALyEGFP1 compl; Length: 105" 0 (CStringList) 0) 
COMMENT         #52=(COligo "GAL1 promoter" 
COMMENT              "AAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 384" 0 (CStringList) 0) 
COMMENT         #53=(COligo "GAL10/CYC1 promoter" 
COMMENT              "ATAACCATAAAAGCTAGTATTGTAGAATCTTTATTGTTCGGAGCAGTGCGGCGCGAGGCACATCTGCGTTTCAGGAACGCGACCGGTGAAGACGAGGACGCACGGAGGAGAGTCTTCCTTCGGAGGGCTGTCACCCGCTCGGCGGCTTCTAATCCGTACTTCAATATAGCAATGAGCAGTTAAGCGTATTACTGAAAGTTCCAAAGAGAAGGTTTTTTTAGGCTAATCGACCTCGAGCAGATCCGCCAGGCGTGTATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCTCTAAATATTCTTTCCTTATACAT" 
COMMENT              "Fragment of pEMBLyex4; Length: 408" 0 (CStringList) 0) 
COMMENT         #54=(COligo "GALad" 
COMMENT              "ATGGATAAAGCGGAATTAATTCCCGAGCCTCCAAAAAAGAAGAGAAAGGTCGAATTGGGTACCGCCGCCAATTTTAATCAAAGTGGGAATATTGCTGATAGCTCATTGTCCTTCACTTTCACTAACAGTAGCAACGGTCCGAACCTCATAACAACTCAAACAAATTCTCAAGCGCTTTCACAACCAATTGCCTCCTCTAACGTTCATGATAACTTCATGAATAATGAAATCACGGCTAGTAAAATTGATGATGGTAATAATTCAAAACCACTGTCACCTGGTTGGACGGACCAAACTGCGTATAACGCGTTTGGAATCACTACAGGGATGTTTAATACCACTACAATGGATGATGTATATAACTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAG" 
COMMENT              "Fragment of pGAD-mCASP-11; Length: 408" 0 (CStringList) 0) 
COMMENT         #55=(COligo "GALbd" 
COMMENT              "ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCG" 
COMMENT              "Fragment of pAS3; Length: 441" 0 (CStringList) 0) 
COMMENT         #56=(COligo "GALL promoter" 
COMMENT              "TGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of p415GALLmBAX; Length: 369" 0 (CStringList) 0) 
COMMENT         #57=(COligo "GAP promoter" 
COMMENT              "CTTTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACGGCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGCCCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAAACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAATTTATTTGTCCCTATTTCAATCAATTGAACAACTAT" 
COMMENT              "Fragment of pGAPZMFManHDEL; Length: 483" 0 (CStringList) 0) 
COMMENT         #58=(COligo "GST" 
COMMENT              "ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAA" 
COMMENT              "Fragment of pGEXGSTp65(12-317); Length: 654" 0 (CStringList) 0) 
COMMENT         #59=(COligo "GUS start" 
COMMENT              "ATGGTCCGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAACTGTGGAATTGATCAGCGTT" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #60=(COligo "GUS stop" 
COMMENT              "CACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATAA" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #61=(COligo "HA epitope" "TAYCCNTAYGAYGTNCCNGAYTAYGCN" 
COMMENT              "Fragment of pAS2; Length: 27" 0 (CStringList) 0) 
COMMENT         #62=(COligo "hBID" 
COMMENT              "ATGGACTGTGAGGTCAACAACGGTTCCAGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCTTCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCACTCCCGCTTGGGAAGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGA" 
COMMENT              "Fragment of pAS2hBID; Length: 588" 0 (CStringList) 0) 
COMMENT         #63=(COligo "hCMV-IE enhancer" 
COMMENT              "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCA" 
COMMENT              "Fragment of pCAGGS-FLAGAVPShOMI; Length: 378" 0 (CStringList) 0) 
COMMENT         #64=(COligo "hCMV-IE promoter and enhancer" 
COMMENT              "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT" 
COMMENT              "Fragment of pCDM8; Length: 584" 0 (CStringList) 0) 
COMMENT         #65=(COligo "HDEL-tag" "CAYGAYGARYTN" 
COMMENT              "Fragment of pGAPZScgls2a-HDEL; Length: 12" 0 (CStringList) 0) 
COMMENT         #66=(COligo "hEF1 promoter (1)" 
COMMENT              "GCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACT" 
COMMENT              "Fragment of pEF1-hOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #67=(COligo "hEF1 promoter (2)" 
COMMENT              "GAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGA" 
COMMENT              "Fragment of pEF1-hOMI; Length: 144" 0 (CStringList) 0) 
COMMENT         #68=(COligo "His-tag" "CAYCAYCAYCAYCAYCAY" "" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #69=(COligo "HIS3 start" 
COMMENT              "ATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAAATGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCGATAGAGCACTCGA" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #70=(COligo "HIS3 stop" 
COMMENT              "TAGTGAGAGTGCGTTCAAGGCTCTTGCGGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCTCCACCAAAGGTGTTCTTATGTAG" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #71=(COligo "HIS4 terminator" 
COMMENT              "TTATTTAGAGATTTTAACTTACATTTAGATTCGATAGATCC" 
COMMENT              "Fragment of pGlycoSwitchM5; Length: 41" 0 (CStringList) 0) 
COMMENT         #72=(COligo "hOMI (1)" 
COMMENT              "ATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGACCCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTCACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACTCTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCTCGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCC" 
COMMENT              "Fragment of pEF1-hOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #73=(COligo "hOMI (2)" 
COMMENT              "CTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTGCTGAACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGA" 
COMMENT              "Fragment of pEF1-hOMI; Length: 377" 0 (CStringList) 0) 
COMMENT         #74=(COligo "HSV-TK polyA" 
COMMENT              "AACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCAC" 
COMMENT              "Fragment of pEYFP-N1; Length: 70" 0 (CStringList) 0) 
COMMENT         #75=(COligo "HSV-TK promoter" 
COMMENT              "CCCCGCCCAGCGTCTTGTCATTGGCGAATTCGAACACGCAGATGCAGTCGGGGCGGCGCGGTCCCAGGTCCACTTCGCATATTAAGGTGACGCGTGTGGCCTCGA" 
COMMENT              "Fragment of p220.2; Length: 105" 0 (CStringList) 0) 
COMMENT         #76=(COligo "htBID" 
COMMENT              "GGCAACCGCAGCAGCCACTCCCGCTTGGGAAGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGA" 
COMMENT              "Fragment of pAS2htBID; Length: 408" 0 (CStringList) 0) 
COMMENT         #77=(COligo "hygromycin (1)" 
COMMENT              "ATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCA" 
COMMENT              "Fragment of pREP4FLAG; Length: 1000" 0 (CStringList) 0) 
COMMENT         #78=(COligo "hygromycin (2)" "GCACTCGTCCGAGGGCAAAGGAATAG" 
COMMENT              "Fragment of pREP4FLAG; Length: 26" 0 (CStringList) 0) 
COMMENT         #79=(COligo "intron AG promoter" 
COMMENT              "GTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGGGCTCGGGGGAAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAG" 
COMMENT              "Fragment of pCAGGS-FLAGAVPShOMI; Length: 925" 0 (CStringList) 0) 
COMMENT         #80=(COligo "intron hEF1 promoter" 
COMMENT              "GTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAG" 
COMMENT              "Fragment of pEF1-hOMI; Length: 939" 0 (CStringList) 0) 
COMMENT         #81=(COligo "IRES start" 
COMMENT              "CCCCCCCCCCCCCCCCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCC" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #82=(COligo "IRES stop" 
COMMENT              "CTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAA" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #83=(COligo "kanamycin" 
COMMENT              "ATGAGCCATATTCAACGGGAAACGTCTTGCTCAAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGTTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA" 
COMMENT              "Fragment of pRH001; Length: 816" 0 (CStringList) 0) 
COMMENT         #84=(COligo "lac operator NIET APART AANDUIDEN" "TTGTGAGCGGATAACAATT" 
COMMENT              "Fragment of pCAL-c; Length: 19     SAMEN MET PROMOTER AANDUIDEN! zie T7" 
COMMENT              0 (CStringList) 0) 
COMMENT         #85=(COligo "lac promoter" 
COMMENT              "GCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGG" 
COMMENT              "from pUC18; 84 bp." 0 (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #86=(COligo "lacA" 
COMMENT              "TTGAACATGCCAATGACCGAAAGAATAAGAGCAGGCAAGCTATTTACCGATATGTGCGAAGGCTTACCGGAAAAAAGACTTCGTGGGAAAACGTTAATGTATGAGTTTAATCACTCGCATCCATCAGAAGTTGAAAAAAGAGAAAGCCTGATTAAAGAAATGTTTGCCACGGTAGGGGAAAACGCCTGGGTAGAACCGCCTGTCTATTTCTCTTACGGTTCCAACATCCATATAGGCCGCAATTTTTATGCAAATTTCAATTTAACCATTGTCGATGACTACACGGTAACAATCGGTGATAACGTACTGATTGCACCCAACGTTACTCTTTCCGTTACGGGACACCCTGTACACCATGAATTGAGAAAAAACGGCGAGATGTACTCTTTTCCGATAACGATTGGCAATAACGTCTGGATCGGAAGTCATGTGGTTATTAATCCAGGCGTCACCATCGGGGATAATTCTGTTATTGGCGCGGGTAGTATCGTCACAAAAGACATTCCACCAAACGTCGTGGCGGCTGGCGTTCCTTGTCGGGTTATTCGCGAAATAAACGACCGGGATAAGCACTATTATTTCAAAGATTATAAAGTTGAATCGTCAGTTTAA" 
COMMENT              "Fragment of pLacDN10HIFNG; Length: 612" 0 (CStringList) 0) 
COMMENT         #87=(COligo "lacI promoter" 
COMMENT              "GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of pREP4; Length: 78" 0 (CStringList) 0) 
COMMENT         #88=(COligo "lacI start" 
COMMENT              "GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #89=(COligo "lacI stop" 
COMMENT              "CACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #90=(COligo "lacIq promoter" 
COMMENT              "GACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of p714; Length: 78" 0 (CStringList) 0) 
COMMENT         #91=(COligo "lacUV5 promoter" 
COMMENT              "CACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGG" 
COMMENT              "Fragment of p714; Length: 57" 0 (CStringList) 0) 
COMMENT         #92=(COligo "lacZ RBS" "AATTGTGAGCGGATAACAATTTCACACAGGAAACAGCT" 
COMMENT              "Fragment of pGBF-NLScre; Length: 38" 0 (CStringList) 0) 
COMMENT         #93=(COligo "lacZ start" 
COMMENT              "ATGGGGCCCAAGAAGAAACGCAAAGTGGGGAGCATGGGGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #94=(COligo "lacZ stop" 
COMMENT              "GGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTACAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAA" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #95=(COligo "LEU2 start" 
COMMENT              "ATGTTTGTTCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTC" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #96=(COligo "LEU2 stop" 
COMMENT              "TTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAA" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #97=(COligo "loxP" "ATAACTTCGTATAGCATACATTATACGAAGTT" 
COMMENT              "Fragment of pCALNL5; Length: 32" 0 (CStringList) 0) 
COMMENT         #98=(COligo "LUC start" 
COMMENT              "ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCGCTAGAGGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACG" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #99=(COligo "LUC stop" 
COMMENT              "TGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAA" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #100=(COligo "luxA (1)" 
COMMENT               "ATGAAGTTTGGAAATATTTGTTTTTCGTATCAACCACCAGGTGAAACTCATAAGCAAGTAATGGATCGCTTTGTTCGGCTTGGTATCGCCTCAGAAGAGGTAGGGTTTGATACATATTGGACCTTAGAACATCATTTTACAGAGTTTGGTCTTACGGGAAATTTATTTGTTGCTGCGGCTAACCTGTTAGGAAGAACTAAAACATTAAATGTTGGCACTATGGGGGTTGTTATTCCGACAGCACACCCAGTTCGACAGTTAGAAGACGTTTTATTATTAGATCAAATGTCGAAAGGTCGTTTTAATTTTGGAACCGTTCGAGGGCTATACCATAAAGATTTTCGAGTATTTGGTGTTGATATGGAAGAGTCTCGAGCAATTACTCAAAATTTCTACCAGATGATAATGGAAAGCTTACAGACAGGAACCATTAGCTCTGATAGTGATTACATTCAATTTCCTAAGGTTGATGTATATCCCAAAGTGTACTCAAAAAATGTACCAACCTGTATGACTGCTGAGTCCGCAAGTACGACAGAATGGCTAGCAATACAAGGGCTACCAATGGTTCTTAGTTGGATTATTGGTACTAATGAAAAAAAAGCACAGATGGAACTCTATAATGAAATTGCGACAGAATATGGTCATGATATATCTAAAATAGATCATTGTATGACTTATATTTGTTCTGTTGATGATGATGCACAAAAGGCGCAAGATGTTTGTCGGGAGTTTCTGAAAAATTGGTATGACTCATATGTAAATGCGACCAATATCTTTAATGATAGCAATCAAACTCGTGGTTATGATTATCATAAAGGTCAATGGCGTGATTTTGTTTTACAAGGACATACAAACACCAATCGACGTGTTGATTATAGCAATGGTATTAACCCTGTAGGCACTCCTGAGCAGTGTATTGAAATCATTCAACGTGATATTGATGCAACGGGTATTACAAACATTACATGCGGATTTGAAGCTAATGGAACTGAAGATG" 
COMMENT               "Fragment of pJE202; Length: 1000" 0 (CStringList) 0) 
COMMENT         #101=(COligo "luxA (2)" 
COMMENT               "AAATAATTGCTTCCATGCGACGCTTTATGACACAAGTCGCTCCTTTCTTAAAAGAACCTAAATAA" 
COMMENT               "Fragment of pJE202; Length: 65" 0 (CStringList) 0) 
COMMENT         #102=(COligo "luxB" 
COMMENT               "ATGAAATTTGGATTATTTTTTCTAAACTTTCAGAAAGATGGAATAACATCTGAAGAAACGTTGGATAATATGGTAAAGACTGTCACGTTAATTGATTCAACTAAATATCATTTTAATACTGCCTTTGTTAATGAACATCACTTTTCAAAAAATGGTATTGTTGGAGCACCTATTACCGCAGCTGGTTTTTTATTAGGGTTAACAAATAAATTACATATTGGTTCATTAAATCAAGTAATTACCACCCATCACCCTGTACGTGTAGCAGAAGAAGCCAGTTTATTAGATCAAATGTCAGAGGGACGCTTCATTCTTGGTTTTAGTGACTGCGAAAGTGATTTCGAAATGGAATTTTTTAGACGTCATATCTCATCAAGGCAACAACAATTTGAAGCATGCTATGAAATAATTAATGACGCATTAACTACAGGTTATTGTCATCCCCAAAACGACTTTTATGATTTTCCAAAGGTTTCAATTAATCCACACTGTTACAGTGAGAATGGACCTAAGCAATATGTATCCGCTACATCAAAAGAAGTCGTCATGTGGGCAGCGAAAAAGGCACTGCCTTTAACATTTAAGTGGGAGGATAATTTAGAAACCAAAGAACGCTATGCAATTCTATATAATAAAACAGCACAACAATATGGTATTGATATTTCGGATGTTGATCATCAATTAACTGTAATTGCGAACTTAAATGCTGATAGAAGTACGGCTCAAGAAGAAGTGAGAGAATACTTAAAAGACTATATCACTGAAACTTACCCTCAAATGGACAGAGATGAAAAAATTAACTGCATTATTGAAGAGAATGCAGTTGGGTCTCATGATGACTATTATGAATCGACAAAATTAGCAGTGGAAAAAACAGGGTCTAAAAATATTTTATTATCCTTTGAATCAATGTCCGATATTAAAGATGTAAAAGATATTATTGATATGTTGAACCAAAAAATCGAAATGAATTTACCATAA" 
COMMENT               "Fragment of pJE202; Length: 981" 0 (CStringList) 0) 
COMMENT         #103=(COligo "luxC (1)" 
COMMENT               "ATGAATAAATGTATTCCAATGATAATTAATGGAATGATTCAAGATTTTGATAATTATGCATATAAAGAAGTTAAACTAAATAATGATAATAGAGTAAAATTATCTGTCATTACTGAAAGTTCAGTTTCAAAAACATTAAATATCAAAGATAGAATTAATCTAAATTTAAATCAGATTGTGAATTTTTTATATACCGTTGGTCAACGATGGAAAAGTGAAGAATATAATCGGCGACGAACCTATATCCGTGAGTTAAAAACATATCTTGGTTATTCTGATGAAATGGCAAGATTAGAAGCGAATTGGATTGCAATGTTATTGTGCTCTAAAAGTGCTTTGTATGACATTGTTAATTATGATTTGGGCTCTATACACGTATTAGATGAATGGCTTCCACGTGGTGATTGCTATGTTAAAGCACAACCGAAAGGTGTTTCTGTTCACTTGTTAGCTGGTAATGTTCCATTATCAGGAGTGACATCTATTTTGCGTGCTATTTTAACAAAAAATGAGTGCATTATTAAAACTTCGTCTTCAGATCCTTTTACTGCAAACGCTTTAGTTTCCAGTTTTATTGATGTTAATGCAGACCATCCAATAACCAAATCAATGTCTGTTATGTATTGGCCGCATGATGAAGATATGACTCTATCTCAAAGAATAATGAATCATGCCGACGTGGTTATTGCTTGGGGTGGAGACGAGGCGATTAAATGGGCGGTAAAATATTCACCACCGCATGTCGATATTCTGAAATTTGGACCAAAGAAAAGCTTAAGTATTATTGAAGCTCCTAAAGATATAGAAGCCGCAGCAATGGGGGTTGCTCATGATATTTGTTTCTATGACCAGCAAGCCTGCTTCTCTACTCAAGACGTTTATTATATAGGAGATAATTTACCTTTATTTTTAAATGAACTTGAAAAACAGCTAGATCGATACGCGAAAATTTTACCAAAAGGTTCAAATAGTTTTGATGAAAAAGCGGCGTTTACTCTTA" 
COMMENT               "Fragment of pJE202; Length: 1000" 0 (CStringList) 0) 
COMMENT         #104=(COligo "luxC (2)" 
COMMENT               "CTGAAAAAGAAAGTCTATTTGCTGGATATGAAGTGAGAAAGGGAGATAAGCAAGCTTGGTTAATAGTCGTATCACCTACAAATAGCTTTGGAAATCAACCGCTATCACGAAGTGTGTATGTTCATCAAGTATCTGATATTAAAGAGATAATTCCTTTTGTTAATAAAAATAGAACACAAACTGTTTCTATTTATCCTTGGGAAGCGTCATTAAAATATCGAGATAAATTAGCAAGAAGTGGAGTTGAAAGAATTGTTGAATCAGGCATGAATAATATTTTCAGAGTTGGAGGGGCTCATGATTCATTATCTCCTCTCCAGTACCTAGTTAGGTTTGTATCGCATGAGAGACCATTTAATTATACGACAAAAGATGTTGCGGTTGAAATCGAACAAACACGTTACTTAGAGGAAGATAAGTTTTTAGTTTTTGTCCCATAG" 
COMMENT               "Fragment of pJE202; Length: 440" 0 (CStringList) 0) 
COMMENT         #105=(COligo "luxD" 
COMMENT               "ATGAAAGATGAAAGTGCTTTTTTTACGATTGATCACATCATCAAGCTTGATAATGGTCAGTCTATCCGAGTTTGGGAAACACTCCCTAAAAAGAACGTACCAGAGAAAAAACATACAATACTTATTGCTTCGGGTTTTGCTAGAAGAATGGATCATTTTGCAGGTCTTGCTGAGTATTTATCTACTAACGGTTTTCATGTCATTCGCTACGATTCTTTGCATCATGTTGGATTAAGCAGTGGATGTATAAATGAATTTACGATGTCGATTGGAAAAAATAGCCTGCTTACAGTCGTAGATTGGCTTACAGATCATGGTGTCGAACGAATAGGGCTGATTGCTGCTAGTTTGTCAGCGAGAATCGCCTATGAGGTAGTAAATAAAATTAAATTATCATTTTTAATTACGGCCGTAGGTGTCGTTAATCTTAGAGATACATTAGAAAAAGCATTGGAGTATGACTATTTGCAATTACCTATTTCAGAGTTACCAGAAGATCTTGACTTTGAAGGTCATAATTTAGGATCGGAGGTCTTTGTTACAGATTGCTTTAAACATAATTGGGACACGTTAGACTCGACACTTAATAGTGTTAAAGGATTAGCGATTCCATTTATTGCTTTTACTGCAAACGATGATAGTTGGGTAAAGCAAAGTGAAGTTATAGAGCTCATTGATAGCATTGAATCTAGTAATTGTAAGCTCTATTCGCTAATTGGAAGTTCACATGATCTTGGGGAAAATTTGGTTGTATTAAGAAATTTTTATCAATCAGTGACGAAGGCAGCCTTAGCATTAGATGATGGTTTATTGGATTTAGAGATAGACATTATTGAACCTCGATTTGAGGACGTTACAAGTATTACTGTTAAGGAGCGTAGATTAAAAAATGAAATTGAAAATGAATTATTAGAATTGGCTTAA" 
COMMENT               "Fragment of pJE202; Length: 924" 0 (CStringList) 0) 
COMMENT         #106=(COligo "luxE (1)" 
COMMENT               "ATGACTGTCCATACTGAATATAAAAGAAATCAAATCATTGCTAGTTCAGAAATTGATGATCTTATCTTTATGACGAAACCACAAGAGTGGTCATTTGAAGAGCAAAAAGAAATACGGGATAAATTAGTTCGTGAGGCTTTTTATTTTCACTACAATAGAAATGAAGAATATAGAAATTATTGTATCAATCAGCATGTGAGTGATAATTTACACACTATTGATGAAATACCCGTGTTTCCAACATCTGTTTTTAAATATAAGAAATTACATACTGTCACAGCCGAGGACATTGAAAATTGGTATACAAGTAGTGGAACTCGTGGAGTAAAAAGTCATATTGCACGTGATCGTCTTAGCATTGAACGCTTGCTTGGTTCTGTCAACTTCGGAATGAAATACGTTGGAGATTGGTTTGAGCATCAAATGGAATTGATAAATTTAGGACCAGATAGATTCAATACAAATAATATTTGGTTTAAATATGTCATGAGTTTGGTCGAGTTACTTTATCCGACTGAATTTACAGTTGATAATGACAAAATAGATTTTGAAAAAACAGTAAAACATCTATTTAGAATTAAGAATAGTAAAAAAGACATTTGCTTAATTGGGCCACCATTTTTTGTGTATCTTTTGTGCCAATATATGAAAGAAAACAATATTGAATTTAAAGGAGGAGATAGAGTACATATTATTACTGGTGGAGGATGGAAATCTAATCAGAATGACTCTTTAGATCGTGCTGATTTTAATCAATTATTAATGGATACTTTCCAACTCGACAAGATTAATCAAATTAGAGATACCTTTAATCAAGTTGAGCTTAATACTTGTTTTTTTGAAGATGAATTTCAAAGAAAACATGTTCCACCGTGGGTATATGCTCGGGCTCTTGATCCTGAAACCTTGAAACCCGTAGCAGATGGTGAGATCGGGTTGTTAAGTTATATGGACGCCTCATCAACTGCTTACCCTGCTTTTATTGTTACTGATGATATCG" 
COMMENT               "Fragment of pJE202; Length: 1000" 0 (CStringList) 0) 
COMMENT         #107=(COligo "luxE (2)" 
COMMENT               "GTATTGTAAAAGAAATTAGAGAACCAGATCCTTACCCAGGGGTAACTGTTGAGATTGTTCGGCGCTTAAATACACGTGCGCAAAAAGGATGCGCGCTCTCTATGGCTAATGTCATACAAAAGAATATCAAGGATTAA" 
COMMENT               "Fragment of pJE202; Length: 137" 0 (CStringList) 0) 
COMMENT         #108=(COligo "luxG" 
COMMENT               "ATGATTGTTGATGGTAGAGTTTCAAAAATAGTATTAGCGTCAATAAGAAATAATATATATAAGGTATTTATTACTGTAAATTCACCAATAAAGTTCATCGCTGGACAATTTGTAATGGTCACGATTAATGGGAAAAAATGCCCTTTTTCAATTGCGAATTGCCCGACAAAAAATTACGAAATAGAATTGCATATTGGTAGTTCGAATAGAGACTGCTCATTGGATATTATCGAATATTTTGTCGATGCTCTTGTTGAGGAAGTCGCAATTGAGTTAGATGCTCCCCATGGAAACGCTTGGTTACGGTCTGAAAGTAATAACCCATTGCTATTAATTGCGGGAGGTACAGGTTTATCATATATAAATAGCATTCTAACAAATTGCTTAAATAGGAATATACCTCAAGATATTTATCTTTACTGGGGAGTAAAAGACAGTTCTCTTTTGTATGAAGATGAAGAGTTACTAAACTTATCACTAAACAACAAAAACTTTCATTATATTCCTGTTATTGAAGATAAAAGTGAAGAATGGATAGGGAGAAAAGGCACTGTTCTTGATGCTGTCATGGAAGATTTTACTGATCTTACTTATTTTGATATTTATGTTTGTGGACCCTTCATGATGGCTAAAACAGCAAAAGAAAAATTAATTGAAGAGAAAAAAGCAAAGTCAGAACAGATGTTTGCCGATGCTTTTGCATACGTATAA" 
COMMENT               "Fragment of pJE202; Length: 711" 0 (CStringList) 0) 
COMMENT         #109=(COligo "luxI" 
COMMENT               "ATGACTATAATGATAAAAAAATCGGATTTTTTGGCAATTCCATCGGAGGAGTATAAAGGTATTCTAAGTCTTCGTTATCAAGTGTTTAAGCAAAGACTTGAGTGGGACTTAGTTGTAGAAAATAACCTTGAATCAGATGAGTATGATAACTCAAATGCAGAATATATTTATGCTTGTGATGATACTGAAAATGTAAGTGGATGCTGGCGTTTATTACCTACAACAGGTGATTATATGCTGAAAAGTGTTTTTCCTGAATTGCTTGGTCAACAGAGTGCTCCCAAAGATCCTAATATAGTCGAATTAAGTCGTTTTGCTGTAGGTAAAAATAGCTCAAAGATAAATAACTCTGCTAGTGAAATTACAATGAAACTATTTGAAGCTATATATAAACACGCTGTTAGTCAAGGTATTACAGAATATGTAACAGTAACATCAACAGCAATAGAGCGATTTTTAAAGCGTATTAAAGTTCCTTGTCATCGTATTGGAGACAAAGAAATTCATGTATTAGGTGATACTAAATCGGTTGTATTGTCTATGCCTATTAATGAACAGTTTAAAAAAGCAGTCTTAAATTAA" 
COMMENT               "Fragment of pJE202; Length: 582" 0 (CStringList) 0) 
COMMENT         #110=(COligo "luxICDABEG terminator" 
COMMENT               "AGAGAATATAAAAAGCCAGATTATTAATCCGGCTT" 
COMMENT               "Fragment of pJE202; Length: 35" 0 (CStringList) 0) 
COMMENT         #111=(COligo "luxR" 
COMMENT               "ATGAAAAACATAAATGCCGACGACACATACAGAATAATTAATAAAATTAAAGCTTGTAGAAGCAATAATGATATTAATCAATGCTTATCTGATATGACTAAAATGGTACATTGTGAATATTATTTACTCGCGATCATTTATCCTCATTCTATGGTTAAATCTGATATTTCAATTCTAGATAATTACCCTAAAAAATGGAGGCAATATTATGATGACGCTAATTTAATAAAATATGATCCTATAGTAGATTATTCTAACTCCAATCATTCACCAATTAATTGGAATATATTTGAAAACAATGCTGTAAATAAAAAATCTCCAAATGTAATTAAAGAAGCGAAAACATCAGGTCTTATCACTGGGTTTAGTTTCCCTATTCATACGGCTAACAATGGCTTCGGAATGCTTAGTTTTGCACATTCAGAAAAAGACAACTATATAGATAGTTTATTTTTACATGCGTGTATGAACATACCATTAATTGTTCCTTCTCTAGTTGATAATTATCGAAAAATAAATATAGCAAATAATAAATCAAACAACGATTTAACCAAAAGAGAAAAAGAATGTTTAGCGTGGGCATGCGAAGGAAAAAGCTCTTGGGATATTTCAAAAATATTAGGCTGCAGTGAGCGTACTGTCACTTTCCATTTAACCAATGCGCAAATGAAACTCAATACAACAAACCGCTGCCAAAGTATTTCTAAAGCAATTTTAACAGGAGCAATTGATTGCCCATACTTTAAAAATTAA" 
COMMENT               "Fragment of pJE202; Length: 753" 0 (CStringList) 0) 
COMMENT         #112=(COligo "M13 ori" 
COMMENT               "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT               "Fragment of pCS4; Length: 456" 0 (CStringList) 0) 
COMMENT         #113=(COligo "mABIN-2 (1)" 
COMMENT               "ATGTCGTCTGGGGACCCAAGGTCTGGTAGACAGGACGGGGCCCCGCGTGCGGCCGCAGCGCTCTGTGGCCTGTACCACGAGGCCGGCCAGCAACTACAGCGCCTGAAGGATCAGCTGGCCGCGCGTGACGCCCTCATCGCGAGCCTCCGCACCCGCCTCGCGGCTCTGGAAGGGCACACGGCGCCGTCACTCGTGGACGCACTTCTGGATCAGGTGGAGCGCTTCCGTGAGCAGCTGCGACGACAGGAGGAAGGCGCTTCGGAGACCCAGCTGCGGCAGGAAGTTGAAAGACTTACGGAGCGTCTAGAGGAAAAAGAGAGGGAGATGCAACAGCTGATGAGCCAGCCTCAGCATGAGCAAGAGAAGGAGGTAGTCTTGCTTCGGCGAAGTGTGGCAGAGAAGGAGAAAGCCAGGGCCGCCAGTGATGTTCTGTGCCGCTCCTTGGCTGATGAGACCCACCAACTGCGCAGGACATTGGCAGCCACTGCCCACATGTGCCAACATCTGGCCAAATGTCTGGATGAACGACAGTGTGCACAGGGAGACGCTGGGGAGAAAAGCCCTGCTGAGCTAGAGCAAACAAGCAGCGATGCTTCTGGCCAGAGTGTTATTAAGAAGTTACAGGAAGAAAATCGACTGTTAAAACAGAAGGTGACTCATGTAGAAGACCTCAATGCTAAGTGGCAGCGTTATGATGCAAGTAGGGACGAATATGTGAAGGGGTTGCATGCCCAGCTAAAGAGGCGGCAGGTCCCTCTGGAGCCTGAGCTGATGAAGAAGGAGATTTCCCGACTTAACAGACAGTTGGAGGAGAAAATAAGTGACTGTGCGGAAGCAAACCAGGAGCTGACAGCCATGAGGATGTCCCGGGACACTGCGCTGGAGCGAGTGCAGATGCTAGAACAGCAGATTCTTGCTTACAAGGATGACTTCAAATCAGAAAGGGCAGATCGGGAACGAGCGCACAGTAGGATTCAAGAGCTGGAGGAAAAGATCATGT" 
COMMENT               "Fragment of pAS2-mABIN-2new; Length: 1000" 0 (CStringList) 0) 
COMMENT         #114=(COligo "mABIN-2 (2)" 
COMMENT               "CCTTGATGTACCAAGTGTCCCAGAGACAGGACTCCCGGGAGCCAGGACCCTGTCGGATTCATACGGGGAACAAAACTGCCAAGTACTTAGAGATGGATGCACTGGAGCATGTGACCCCTGGCGGCTGGAGGCCTGAGTCTAGGTCCCAACAGATGGAACCTTCTGCAGAGGGTGGGCATGTGTGCACAGCCCAGAGAGGTCAGGGTGACCTTCAGTGCCCTCATTGCCTGCGGTGCTTCAGTGATGAGCAAGGCGAGGCATTCCTCAGGCACCTGTCTGAGTGCTGCCAATGA" 
COMMENT               "Fragment of pAS2-mABIN-2new; Length: 293" 0 (CStringList) 0) 
COMMENT         #115=(COligo "mBAX" 
COMMENT               "ATGGACGGGTCCGGGGAGCAGCTTGGGAGCGGCGGGCCCACCAGCTCTGAACAGATCATGAAGACAGGGGCCTTTTTGCTACAGGGTTTCATCCAGGATCGAGCAGGGAGGATGGCTGGGGAGACACCTGAGCTGACCTTGGAGCAGCCGCCCCAGGATGCGTCCACCAAGAAGCTGAGCGAGTGTCTCCGGCGAATTGGAGATGAACTGGATAGCAATATGGAGCTGCAGAGGATGATTGCTGACGTGGACACGGACTCCCCCCGAGAGGTCTTCTTCCGGGTGGCAGCTGACATGTTTGCTGATGGCAACTTCAACTGGGGCCGCGTGGTTGCCCTCTTCTACTTTGCTAGCAAACTGGTGCTCAAGGCCCTGTGCACTAAAGTGCCCGAGCTGATCAGAACCATCATGGGCTGGACACTGGACTTCCTCCGTGAGCGGCTGCTTGTCTGGATCCAAGACCAGGGTGGCTGGGAAGGCCTCCTCTCCTACTTCGGGACCCCCACATGGCAGACAGTGACCATCTTTGTGGCTGGAGTCCTCACCGCCTCGCTCACCATCTGGAAGAAGATGGGCTGA" 
COMMENT               "Fragment of pRS415GAL1mBAX; Length: 579" 0 (CStringList) 0) 
COMMENT         #116=(COligo "mcPLA2 (1)" 
COMMENT               "ATGTCTTTCATAGATCCTTATCAGCACATTATAGTGGAACACCAGTACTCCCATAAGTTTACTGTTGTGGTTCTACGTGCCACCAAAGTAACCAAGGGGACCTTTGGCGATATGCTGGACACTCCAGATCCTTATGTGGAACTTTTCATCTCTACAACCCCTGACAGCAGGAAGCGAACGAGACACTTCAATAATGATATAAACCCCGTGTGGAATGAGACCTTTGAGTTCATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATCACACTGATGGATGCCAACTACGTCATGGATGAAACCCTAGGCACAGCTACATTCCCTGTATCTTCAATGAAAGTGGGAGAGAAGAAAGAAGTCCCTTTTATTTTCAACCAAGTCACTGAAATGATTCTGGAAATGTCTCTGGAAGTTTGTTCATGCCCAGACCTACGGTTCAGCATGGCACTGTGTGATCAGGAGAAAACTTTCAGACAGCAGAGGAAAGAGAACATAAAAGAGAACATGAAGAAACTTTTGGGTCCAAAAAAGAGTGAGGGGCTTTATTCCACACGTGATGTGCCGGTGGTGGCCATTTTGGGTTCAGGTGGGGGTTTCCGGGCCATGGTGGGATTCTCTGGTGTGATGAAGGCACTGTATGAGTCGGGGATTTTGGACTGTGCTACATACATTGCTGGTCTTTCTGGATCCACATGGTACATGTCAACCTTGTACTCTCACCCCGATTTTCCAGAGAAAGGTCCCGAGGAGATTAATGAAGAGCTAATGAAAAATGTCAGCCACAACCCTCTCTTACTTCTTACACCACAGAAAGTTAAAAGATACGTTGAGTCTTTATGGAAGAAGAAAAGTTCTGGCCAGCCTGTCACCTTTACTGACATCTTTGGGATGCTAATAGGAGAAACACTAATTCAAAATAGGATGAGCATGACCCTGAGTAGTTTGAAGGAAAAGGTCAATGCCGCCCGGTGTCCTTTGCCTCTCTTCACGTGTCTCCACG" 
COMMENT               "Fragment of pEF6-Etag-mcPLA2; Length: 1000" 0 (CStringList) 0) 
COMMENT         #117=(COligo "mcPLA2 (2)" 
COMMENT               "TCAAACCTGATGTGTCAGAGCTGATGTTTGCCGATTGGGTGGAATTTAGTCCATATGAGATTGGCATGGCAAAATATGGTACCTTTATGGCTCCTGACCTATTTGGAAGCAAGTTTTTTATGGGAACAGTTGTAAAAAAATATGAAGAAAACCCCTTGCATTTCTTGATGGGTGTCTGGGGCAGTGCCTTTTCTATACTGTTCAACAGAGTTTTGGGAGTTTCTGGCTCACAGAATAAAGGCTCTACAATGGAAGAGGAATTAGAAAATATTACAGCAAAGCACATCGTGAGTAATGACAGCTCCGACAGTGATGATGAGGCTCAAGGACCCAAAGGCACCGAGAATGAAGAAGCTGAAAAAGAGTACCAAAGCGACAACCAAGCAAGTTGGGTCCATCGGATGCTAATGGCCTTGGTGAGCGACTCGGCTTTATTCAATACCCGAGAAGGACGTGCCGGAAAGGTGCATAACTTCATGCTGGGCTTGAATCTCAACACATCATATCCACTGTCTCCCCTGAGAGACTTCAGCTCTCAGGATTCCTTCGATGACGAGCTCGACGCAGCGGTAGCAGATCCAGATGAATTTGAACGAATATATGAACCACTGGATGTCAAAAGTAAGAAGATTCATGTGGTAGATAGTGGGCTCACATTTAACCTGCCGTATCCCTTGATTCTGCGACCTCAGAGAGGTGTGGATCTTATCATCTCCTTTGACTTTTCTGCAAGGCCGAGTGACACCAGTCCCCCTTTCAAGGAACTTCTGCTTGCAGAGAAGTGGGCGAAAATGAACAAGCTTCCCTTTCCAAAGATCGATCCTTATGTGTTTGATCGGGAAGGATTAAAGGAATGCTATGTTTTTAAACCTAAGAATCCTGATGTGGAGAAGGATTGCCCAACCATTATCCACTTTGTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCCCCAGGTGTTCTAAGGGAAACCAAAGAAGAGAAAGAAATCGCTGACTT" 
COMMENT               "Fragment of pEF6-Etag-mcPLA2; Length: 1000" 0 (CStringList) 0) 
COMMENT         #118=(COligo "mcPLA2 (3)" 
COMMENT               "TGACATTTTTGATGACCCCGAATCGCCATTTTCAACCTTCAACTTTCAGTATCCCAATCAAGCATTCAAAAGGCTGCATGATCTGATGTACTTCAACACACTGAACAACATTGATGTGATAAAGGATGCCATTGTTGAGAGCATTGAATACAGAAGACAGAACCCATCTCGTTGCTCTGTTTCCCTCAGTAATGTTGAAGCAAGAAAATTCTTCAATAAGGAGTTTCTAAGTAAACCCACTGTGTAA" 
COMMENT               "Fragment of pEF6-Etag-mcPLA2; Length: 247" 0 (CStringList) 0) 
COMMENT         #119=(COligo "mDHFR" 
COMMENT               "ATGGTTCGACCATTGAACTGCATCGTCGCCGTGTCCCAAAATATGGGGATTGGCAAGAACGGAGACCTACCCTGGCCTCCGCTCAGGAACGAGTTCAAGTACTTCCAAAGAATGACCACAACCTCTTCAGTGGAAGGTAAACAGAATCTGGTGATTATGGGTAGGAAAACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGACAGAATTAATATAGTTCTCAGTAGAGAACTCAAAGAACCACCACGAGGAGCTCATTTTCTTGCCAAAAGTTTGGATGATGCCTTAAGACTTATTGAACAACCGGAATTGGCAAGTAAAGTAGACATGGTTTGGATAGTCGGAGGCAGTTCTGTTTACCAGGAAGCCATGAATCAACCAGGCCACCTTAGACTCTTTGTGACAAGGATCATGCAGGAATTTGAAAGTGACACGTTTTTCCCAGAAATTGATTTGGGGAAATATAAACTTCTCCCAGAATACCCAGGCGTCCTCTCTGAGGTCCAGGAGGAAAAAGGCATCAAGTATAAGTTTGAAGTCTACGAGAAGAAAGACTAA" 
COMMENT               "Fragment of pSCUDMFE6L2; Length: 564" 0 (CStringList) 0) 
COMMENT         #120=(COligo "mob'" 
COMMENT               "ATGGCGGCATACGCGATCATGCGATGCAAGAAGCTGGCGAAAATGGGCAACGTGGCGGCCAGTCTCAAGCACGCCTACCGCGAGCGCGAGACGCCCAACGCTGACGCCAGCAGGACGCCAGAGAACGAGCACTGGGCGGCCAGCAGCACCGATGAAGCGATGGGCCGACTGCGCGAGTTGCTGCCAGAGAAGCGGCGCAAGGACGCTGTGTTGGCGGTCGAGTACGTCATGACGGCCAGCCCGGAATGGTGGAAGTCGGCCAGCCAAGAACAGCAGGCGGCGTTCTTCGAGAAGGCGCACAAGTGGCTGGCGGACAAGTACGGGGCGGATCGCATCGTGACGGCCAGCATCCACCGTGACGAAACCAGCCCGCACATGACCGCGTTCGTGGTGCCGCTGACGCAGGACGGCAGGCTGTCGGCCAAGGAGTTCATCGGCAACAAAGCGCAGATGACCCGCGACCAGACCACGTTTGCGGCCGCTGTGGCCGATCTAGGGCTGCAACGGGGCATCGAGGGCAGCAAGGCACGTCACACGCGCATTCAGGCGTTCTACGAGGCCCTGGAGCGGCCACCAGTGGGCCACGTCACCATCAGCCCGCAAGCGGTCGAGCCACGCGCCTATGCACCGCAGGGATTGGCCGAAAAGCTGGGAATCTCAAAGCGCGTTGAGACGCCGGAAGCCGTGGCCGACCGGCTGACAAAAGCGGTTCGGCAGGGGTATGAGCCTGCCCTACAGGCCGCCGCAGGAGCGCGTGAGATGCGCAAGAAGGCCGATCAAGCCCAAGAGACGGCCCGAGACCTTCGGGAGCGCCTGAAGCCCGTTCTGGACGCCCTGGGGCCGTTGAATCGGGATATGCAGGCCAAGGCCGCCGCGATCATCAAGGCCGTGGGCGAAAAGCTGCTGACGGAACAGCGGGAAGTCCAGCGCCAGAAACAGGCCCAGCGCCAGCAGGAACGCGGGCGCG" 
COMMENT               "incomplete but functional mobilization gene; 6 C-terminal codons missing" 
COMMENT               0 (CStringList) 0) 
COMMENT         #121=(COligo "mPGK1 promoter" 
COMMENT               "TTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGA" 
COMMENT               "Fragment of pPNEcad; Length: 508" 0 (CStringList) 0) 
COMMENT         #122=(COligo "Myc epitope" "GARCARAARYTNATHWSNGARGARGAYYTN" 
COMMENT               "Fragment of MYC-TAG; Length: 30" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #123=(COligo "neomycin" 
COMMENT               "ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA" 
COMMENT               "Fragment of pCDNA-mCASP-8; Length: 795" 0 (CStringList) 0) 
COMMENT         #124=(COligo "nos promoter start" 
COMMENT               "GATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGATTGAA" 
COMMENT               "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #125=(COligo "nos promoter stop" 
COMMENT               "AGGTCACTATCAGCTAGCAAATATTTCTTGTCAAAAATGCTCCACTGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAA" 
COMMENT               "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #126=(COligo "ocs terminator start" 
COMMENT               "CTGCTTTAATGAGATATGCGAGACGCCTATGATCGCATGATATTTGCTTTCAATTCTGTTGTGCACGTTGTAAAAAACCTGAGCATGTGTAGCTCAGATC" 
COMMENT               "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #127=(COligo "ocs terminator stop" 
COMMENT               "GTGGGCCCCCAAATGAAGTGCAGGTCAAACCTTGACAGTGACGACAAATCGTTGGGCGGGTCCAGGGCGAATTTTGCGACAACATGTCGAGGCTCAGCAG" 
COMMENT               "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #128=(COligo "p15A ori start" 
COMMENT               "GGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGT" 
COMMENT               "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #129=(COligo "p15A ori stop" 
COMMENT               "GTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTT" 
COMMENT               "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #130=(COligo "parA" 
COMMENT               "TTGAAGGTCGCCCGCATCTACCTGCGCGCCAGTACGGACGAGCAGAATCTTGAACGCCAGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTTGCGGAGAAGATCGACCGCATCAGCCGCTTGCCGTTGGCCGAGGCCGAGCGCCTGGTTGCGTCGATCCGGGCCAAAGGGGCCAAGCTGGCCGTGCCTGGCGTGGTGGACCTGTCGGAGCTGGCCGCCGAGGCGAACGGAGTGGCGAAAATCGTTCTGGAATCCGTCCAGGACATGCTTTTGAAGCTCGCCTTGCAGATGGCCCGCGACGACTACGAGGATCGGCGCGAGCGTCAACGTCAGGGTGTCCAGTTGGCGAAGGCCGCCGGCCGCTACACCGGCCGCAAACGTGACGCCGGCATGCACGACCGCATCATCACGCTTCGCTCCGGCGGATCGAGCATTGCCAAGACGGCCAAGCTGGTCGGATGCAGCCCGAGCCAGGTCAAACGAGTGTGGGCGGCCTGGAACGCGCAGCAGCAAAAATAA" 
COMMENT               "Fragment of pRH001; Length: 762" 0 (CStringList) 0) 
COMMENT         #131=(COligo "parB" 
COMMENT               "ATGAAGCGCCGAAGCTACGCCATGCTGCGCGCCGCTGCCGCGCTGGCCGTCCTGGTCGTTGCCTCGCCGGCATGGGCCGAGCTGCGCGGCGAGGTCGTGCGCATCATCGACGGCGACACCATCGACGTGCTGGTAGACAAGCAGCCGGTGCGCGTGCGCCTGGTGGACATTGACGCGCCGGAAAAGCGGCAAGCCTTCGGCGAACGTGCGCGCCAGGCGCTGGCCGGCATGGTGTTCCGCCGGCACGTCCTGGTCGACGAGAAGGACACCGACCGTTACGGCCGCACGCTGGGCACCGTGTGGGTCAACATGGAGCTGGCCAGCCGGCCGCCGCAGCCGCGCAACGTCAACGCCGCGATGGTTCACCAGGGCATGGCGTGGGCCTATCGCTTCCACGGCCGCGCGGCCGACCCTGAAATGCTGCGGCTCGAACAGGAGGCGCGAGGCAAGCGCGTCGGCCTCTGGTCCGATCCGCACGCCGTCGAGCCGTGGAAATGGCGACGCGAGAGCAACAACCGGAGGGACGAAGGTTGA" 
COMMENT               "Fragment of pRH001; Length: 534" 0 (CStringList) 0) 
COMMENT         #132=(COligo "parC" 
COMMENT               "ATGGGTATACCCAATTTGACCAAGGGGGACGCGATGAGGGCGGCCAAGCACTACCGACAACTTCTATCCATCGACTTCAACATCGAGGCGCTGGCCTTCGTGCCTGGACCCGACGGCACACGCGGCCGGCGCATCCACGTCCTGGGGCGCGAGGTCCGCGACCGGCCCGGCCTGGTCGAGTACCTTTCGCCGGCGTTCGGCTCGCGGGTGGCGCTGGACGGCTACTGCAAGGCCAATTTCGATGCAGTGCTGCACCTGGCGTACCCCGATCATCAGCAATGGGGCCACGCATGA" 
COMMENT               "Fragment of pRH001; Length: 294" 0 (CStringList) 0) 
COMMENT         #133=(COligo "parD" 
COMMENT               "ATGAGCCGCCTGACAATCGACATGACGGACCAGCAGCACCAGAGCCTGAAAGCCCTGGCCGCCTTGCAGGGCAAGACCATTAAGCAATACGCCCTCGAACGTCTGTTCCCCGGTGACGCTGATGCCGATCAGGCATGGCAGGAACTGAAAACCATGCTGGGGAACCGCATCAACGATGGGCTTGCCGGCAAGGTGTCCACCAAGAGCGTCGGCGAAATTCTTGATGAAGAACTCAGCGGGGATCGCGCTTGA" 
COMMENT               "Fragment of pMR10cat; Length: 252" 0 (CStringList) 0) 
COMMENT         #134=(COligo "parE" 
COMMENT               "TTGACGGCCTACATCCTCACGGCTGAGGCCGAAGCCGATCTACGCGGCATCATCCGCTACACGCGCCGGGAGTGGGGCGCGGCGCAGGTGCGCCGCTATATCGCTAAGCTGGAACAGGGCATAGCCAGGCTTGCCGCCGGCGAAGGCCCGTTTAAGGACATGAGCGAACTCTTTCCCGCGCTGCGGATGGCCCGCTGCGAACACCACTACGTTTTTTGCCTGCCGCGTGCGGGCGAACCCGCGTTGGTCGTGGCGATCCTGCATGAGCGCATGGACCTCATGACGCGACTTGCCGACAGGCTCAAGGGCTGA" 
COMMENT               "Fragment of pMR10cat; Length: 312" 0 (CStringList) 0) 
COMMENT         #135=(COligo "pBBR1 ori" 
COMMENT               "ATGGCCACGCAGTCCAGAGAAATCGGCATTCAAGCCAAGAACAAGCCCGGTCACTGGGTGCAAACGGAACGCAAAGCGCATGAGGCGTGGGCCGGGCTTATTGCGAGGAAACCCACGGCGGCAATGCTGCTGCATCACCTCGTGGCGCAGATGGGCCACCAGAACGCCGTGGTGGTCAGCCAGAAGACACTTTCCAAGCTCATCGGACGTTCTTTGCGGACGGTCCAATACGCAGTCAAGGACTTGGTGGCCGAGCGCTGGATCTCCGTCGTGAAGCTCAACGGCCCCGGCACCGTGTCGGCCTACGTGGTCAATGACCGCGTGGCGTGGGGCCAGCCCCGCGACCAGTTGCGCCTGTCGGTGTTCAGTGCCGCCGTGGTGGTTGATCACGACGACCAGGACGAATCGCTGTTGGGGCATGGCGACCTGCGCCGCATCCCGACCCTGTATCCGGGCGAGCAGCAACTACCGACCGGCCCCGGCGAGGAGCCGCCCAGCCAGCCCGGCATTCCGGGCATGGAACCAGACCTGCCAGCCTTGACCGAAACGGAGGAATGGGAACGGCGCGGGCAGCAGCGCCTGCCGATGCCCGATGAGCCGTGTTTTCTGGACGATGGCGAGCCGTTGGAGCCGCCGACACGGGTCACGCTGCCGCGCCGGTAG" 
COMMENT               "" 0 (CStringList) 0) 
COMMENT         #136=(COligo "Pichia URA3" 
COMMENT               "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT               "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #137=(COligo "PL promoter" 
COMMENT               "TCTCTCACCTACCAAACAATGCCCCCCTGCAAAAAATAAATTCATATAAAAAACATACAGATAACCATCTGCGGTGATAAATTATCTCTGGCGGTGTTGACATAAATACCACTGGCGGTGATACTGAGCAC" 
COMMENT               "Fragment of pLT-mCASP-1p30-1; Length: 131" 0 (CStringList) 0) 
COMMENT         #138=(COligo "pMB1 ori" 
COMMENT               "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGC" 
COMMENT               "Fragment of pRH007; Length: 403" 0 (CStringList) 0) 
COMMENT         #139=(COligo "polyoma ori" 
COMMENT               "CTGTTTTTTTTAGTATTAAGCAGAGGCCGGGGACCCCTGGGCCCGCTTACTCTGGAGAAAAAGAAGAGAGGCATTGTAGAGGCTTCCAGAGGCAACTTGTCAAAACAGGACTG" 
COMMENT               "Fragment of pCDM92; Length: 113" 0 (CStringList) 0) 
COMMENT         #140=(COligo "ppMF" 
COMMENT               "ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCT" 
COMMENT               "Fragment of pGAPZMFManHDEL; Length: 243" 0 (CStringList) 0) 
COMMENT         #141=(COligo "puromycin" 
COMMENT               "ATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGA" 
COMMENT               "Fragment of pSVpaz22; Length: 600" 0 (CStringList) 0) 
COMMENT         #142=(COligo "R6K ori" 
COMMENT               "TCTGAAGATCAGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTCAGTGACACAGGAACACTTAACGGCTGACAT" 
COMMENT               "Fragment of pKNG101; Length: 388" 0 (CStringList) 0) 
COMMENT         #143=(COligo "RK2 ori" 
COMMENT               "GGCCGCCGGCGTTGTGGATACCTCGCGGAAAACTTGGCCCTCACTGACAGATGAGGGGCGGACGTTGACACTTGAGGGGCCGACTCACCCGGCGCGGCGTTGACAGATGAGGGGCAGGCTCGATTTCGGCCGGCGACGTGGAGCTGGCCAGCCTCGCAAATCGGCGAAAACGCCTGATTTTACGCGAGTTTCCCACAGATGATGTGGACAAGCCTGGGGATAAGTGCCCTGCGGTATTGACACTTGAGGGGCGCGACTACTGACAGATGAGGGGCGCGATCCTTGACACTTGAGGGGCAGAGTGCTGACAGATGAGGGGCGCACCTATTGACATTTGAGGGGCTGTCCACAGGCAGAAAATCCAGCATTTGCAAGGGTTTCCGCCCGTTTTTCGGCCACCGCTAACCTGTCTTTTAACCTGCTTTTAAACCAATATTTATAAACCTTGTTTTTAACCAGGGCTGCGCCCTGTGCGCGTGACCGCGCACGCCGAAGGGGGGTGCCCCCCCTTCTCGAACCCTCCCGG" 
COMMENT               "Fragment of pRH001; Length: 526" 0 (CStringList) 0) 
COMMENT         #144=(COligo "RK2/RP4 oriT" 
COMMENT               "CGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGG" 
COMMENT               "Fragment of pRH001; Length: 111" 0 (CStringList) 0) 
COMMENT         #145=(COligo "rop" 
COMMENT               "GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA" 
COMMENT               "Fragment of pBR322; Length: 192" 0 (CStringList) 0) 
COMMENT         #146=(COligo "RSV-LTR" 
COMMENT               "GCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACAGGTCTGACATGGATTGGACGAACCACTGAATTCCGCATTGCAGAGATAATTGTATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCACCT" 
COMMENT               "Fragment of pRcRSVp65; Length: 183" 0 (CStringList) 0) 
COMMENT         #147=(COligo "SP6 promoter" "ATTTAGGTGACACTATA" 
COMMENT               "Fragment of pdCS2-MT ; Length: 17" 0 (CStringList) 0) 
COMMENT         #148=(COligo "Strep-tag" "GCTTGGCGTCACCCGCAGTTCGGTGGT" 
COMMENT               "pUChGNTI2: 27 nucleotides" 0 (CStringList) 0) 
COMMENT         #149=(COligo "supF" 
COMMENT               "TCTTTCTCAACGTAACACTTTACAGCGGCGCGTCATTTGATATGATGCGCCCCGCTTCCCGATAAGGGAGCAGGCCAGTAAAAGCATTACCCGTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTCTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCGAAAGAAT" 
COMMENT               "Fragment of pCDM92; Length: 203" 0 (CStringList) 0) 
COMMENT         #150=(COligo "SV40 early promoter" 
COMMENT               "CTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCC" 
COMMENT               "Fragment of pEF6/Myc-HisA; Length: 282" 0 (CStringList) 0) 
COMMENT         #151=(COligo "SV40 ori" 
COMMENT               "GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCKGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTT" 
COMMENT               "Fragment of SV40; Length: 76" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #152=(COligo "SV40 polyA early" 
COMMENT               "AATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTT" 
COMMENT               "Fragment of pdEYFP; Length: 97" 0 (CStringList) 0) 
COMMENT         #153=(COligo "synthetic EM7 promoter" 
COMMENT               "GTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACC" 
COMMENT               "Fragment of pPICZTS; Length: 66, as in the pEF6 plasmids" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #154=(COligo "T1 terminator" 
COMMENT               "ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT" 
COMMENT               "Fragment of pDONR201; Length: 44" 0 (CStringList) 0) 
COMMENT         #155=(COligo "T2 terminator" "AGAAGGCCATCCTGACGGATGGCCTTTT" 
COMMENT               "Fragment of pDONR201; Length: 28" 0 (CStringList) 0) 
COMMENT         #156=(COligo "T3 promoter" "AATTAACCCTCACTAAA" 
COMMENT               "Fragment of pLT-mCASP-14" 0 (CStringList) 0) 
COMMENT         #157=(COligo "T7 promoter" "TAATACGACTCACTATA" 
COMMENT               "Fragment of pGEM-TAX1BP1-S; Length: 17" 0 (CStringList) 0) 
COMMENT         #158=(COligo "T7 promoter with lac operator" 
COMMENT               "TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCC" 
COMMENT               "Fragment of pCAL-c; Length: 44" 0 (CStringList) 0) 
COMMENT         #159=(COligo "T7 RBS" 
COMMENT               "GGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAT" 
COMMENT               "Fragment of pLT10Bgal; Length: 63" 0 (CStringList) 0) 
COMMENT         #160=(COligo "T7 terminator" 
COMMENT               "CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG" 
COMMENT               "Fragment of pGBKT7; Length: 48" 0 (CStringList) 0) 
COMMENT         #161=(COligo "tac promoter" "GTTGACAATTAATCATCGGCTCGTATAATGTGTGG" 
COMMENT               "Fragment of pGEX5X1; Length: 35" 0 (CStringList) 0) 
COMMENT         #162=(COligo "TEF1 E. gossypii promoter" 
COMMENT               "GGTTGTTTATGTTCGGATGTGATGTGAGAACTGTATCCTAGCAAGATTTTAAAAGGAAGTATATGAAAGAAGAACCTCAGTGGCAAATCCTAACCTTTTATATTTCTCTACAGGGGCGCGGCGTGGGGACAATTCAACGCGTCTGTGAGGGGAGCGTTTCCCTGCTCGCAGGTCTGCAGCGAGGAGCCGTAATTTTTGCTTCGCGCCGTGCGGCCATCAAAATGTATGGATGCAAATGATTATACATGGGGATGTATGGGCTAAATGTACGGGCGACAGTCACATCATGCCCCTGAGCTGCGCACGTCAAGACTGTCAAGGAGGGTATTCTGGGCCTCCATGTC" 
COMMENT               "E. gossypii TEF1alpha promoter" 0 (CStringList) 0) 
COMMENT         #163=(COligo "TEF1 promoter" 
COMMENT               "CCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTTCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAATTTTTTTTTTTAGTTTTTTTCTCTTTCAGTGACCTCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGTTCATTAGAAAGA" 
COMMENT               "Fragment of pGlycoSwitchM8; Length: 406" 0 (CStringList) 0) 
COMMENT         #164=(COligo "TEF1 terminator start" 
COMMENT               "AGTAACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATT" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #165=(COligo "TEF1 terminator stop" 
COMMENT               "ATATTTTTTTTGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #166=(COligo "TET start" 
COMMENT               "ATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGG" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #167=(COligo "TET stop" 
COMMENT               "CACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGA" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #168=(COligo "thrombin recognition site" "CTGGTTCCGCGTGGATCC" 
COMMENT               "Fragment of pGEX-4T2; Length: 18" 0 (CStringList) 0) 
COMMENT         #169=(COligo "TRP1" 
COMMENT               "ATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTAGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAG" 
COMMENT               "Fragment of pAS2; Length: 675" 0 (CStringList) 0) 
COMMENT         #170=(COligo "URA3" 
COMMENT               "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT               "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0) 
COMMENT         #171=(COligo "V5 epitope" "GGNAARCCNATHCCNAAYCCNYTNYTNGGNYTNGAYWSNACN" 
COMMENT               "length: 42 bp" 0 (CStringList) 0) 
COMMENT         #172=(COligo "VSV epitope" "TACACCGACATCGAGATGAACCGGTTGGGCAAG" 
COMMENT               "Fragment of pCR3VSV-CED-4; Length: 33" 0 (CStringList) 0) 
COMMENT         #173=(COligo "XGPRT" 
COMMENT               "ATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTAAAGTGCTGAAACGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAA" 
COMMENT               "Fragment of pDelta1; Length: 459" 0 (CStringList) 0) 
COMMENT         #174=(COligo "Xpress epitope" "GAYYTNTAYGAYGAYGAYGAYAAR" 
COMMENT               "Fragment of pYD1TSE ; Length: 24" 0 (CStringList) 0) 
COMMENT         #175=(COligo "zeocin" 
COMMENT               "ATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGA" 
COMMENT               "Fragment of pGAPZMFManHDEL; Length: 375" 0 (CStringList) 0) 
COMMENT         #176=(COligo "\337-globin polyA" 
COMMENT               "GATCTTTTTCCCTCTGCCAAAAATTATGGGGACATCATGAAGCCCCTTGAGCATCTGACTTCTGGSTAATAAAGGAAATTTATTTTCATTGC" 
COMMENT               "Fragment of pBI-G; Length: 92" 0 (CStringList) 0)) 1 "{(0,1),2}" 
COMMENT        0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7591 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #177=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 682 0 7519 0 0 "") 
COMMENT        #178=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 4 3080 0 5518 0 6018 0 
COMMENT              7264 0 0 "") 
COMMENT        #179=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 2151 0 2177 0 0 "") 
COMMENT        #180=(CRSite (CStringList) "BcuI" "actagt" 2 0 1 906 0 0 "") 
COMMENT        #181=(CRSite (CStringList) "BglII" "agatct" 2 0 3 519 0 1578 0 2378 0 0 
COMMENT              "") 
COMMENT        #182=(CRSite (CStringList) "HincII" "gtyrac" 4 0 3 1837 0 3606 0 7138 0 
COMMENT              0 "") 
COMMENT        #183=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 1217 0 2140 0 2155 0 0 
COMMENT              "") 
COMMENT        #184=(CRSite (CStringList) "PaeI" "gcatgc" 6 0 2 1391 0 2575 0 0 "") 
COMMENT        #185=(CRSite (CStringList) "PdmI" "gaannnnttc" 6 0 2 5262 0 7196 0 0 "") 
COMMENT        #186=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 1867 0 6842 0 0 "") 
COMMENT        #187=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "")) 
COMMENT       (CObList 
COMMENT        #188=(CFSignal (CObList) "lacI promoter" 30 0 0 2671 2748 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #189=(CFSignal (CObList) "lacI" 4 0 0 2749 3831 0 (CStringList) 
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FEATURES             Location/Qualifiers
     promoter        2671..2748
                     /vntifkey="30"
                     /label=lacI\promoter
                     /note="E. coli repressor lacI promoter, incl. ribosome binding site"
     promoter        complement(2321..2362)
                     /vntifkey="30"
                     /label=T7\promoter\with\lac\operator
     RBS             complement(2275..2319)
                     /vntifkey="32"
                     /label=5'\UTR\of\T7\gene\10,\incl.\RBS
     CDS             2749..3831
                     /vntifkey="4"
                     /label=lacI
     CDS             5143..5334
                     /vntifkey="4"
                     /label=rop
                     /note="modulation repressor coding sequence"
     rep_origin      complement(5580..5982)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6523..7383)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      complement(213..260)
                     /vntifkey="43"
                     /label=T7\terminator
     misc_marker     complement(2176..2193)
                     /vntifkey="22"
                     /label=thrombin\recognition\site
     misc_marker     complement(2194..2271)
                     /vntifkey="22"
                     /label=calmodulin-binding\peptide\tag
     CDS             complement(331..2142)
                     /vntifkey="4"
                     /label=hAPI2
     mutation        complement(2137..2139)
                     /vntifkey="62"
                     /label=Kozak
                     /note="AAC was replaced by GAC (N2D) creating Kozak consensus sequence GCCACCATGG"
BASE COUNT     1831 a      1951 c      1871 g      1938 t 
ORIGIN
        1 ttgtcatgtt tgacagctta tcttcgatta gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 
      241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 
      301 cgggctttgt tagcagccgg atctaagctc tgaaagaaat gtacgaactg tacccttgat 
      361 tgtactccta caaataggac actttcttaa agaaggagca caatctttgc atactactag 
      421 atgaccacaa ggaataaaca ctatggacac ttctttgtcc atacacactt tacatgttct 
      481 ttcttcttgt agtctccgca attgttcttc cactggtaga tctgaaacat cttctgtggg 
      541 aatatatttt atgtcctgtt gcacaaataa atgctcatat aacacagctt cagcttcttg 
      601 cagagagttt ctgaatacag tggctgcaat atttcctttt actaaaatcg tatcaatcag 
      661 ttctcttgct tgtaaagacg tctgtgtctt ctgtttaata acatcatgtt cttgttcatt 
      721 aataattccg gcagttagta gactatccag gattggaatt acacaagtca aatgttgaaa 
      781 aagtgccatt ctattcttcc ggattaataa taaatcattt gattcttttt cctcagttgc 
      841 tctttctctc tcctcttccc ttatttcatc ttctgcattg agtaagtcta acacaagatc 
      901 attgactagt ctataattct ctccagttgc taggattttt ctctgaactg tctgttttac 
      961 caggcttcta ctaaagccca tttccacggc agcattaatc acaggagtat tcatcatgat 
     1021 tgcatcttct gaatggtctt ctccaggttc aaaatggata attgatgact ctgcattttc 
     1081 atctcctggg ctgtctgatg tggatagcag ctgttcaagt agatgagggt aactggcttg 
     1141 aacttgacgg atgaactcct gtcctttaat tcttatcaag tactcacacc ttggaaacca 
     1201 cttggcatgt tgaacccatg gatcatctcc agattcccaa cacctgagtc caccatcaca 
     1261 gcaaaagcat ttgacatcat cactgttacc cacataataa aaacccgcac ttgcaagctg 
     1321 ctcaggatta actagaacac tagagggcca gttaaagaat gttttaaagc gggctgcatg 
     1381 tgtctgcatg ctcagattag aaactgtgta tcttgaagtg tcttgaagct gattttctat 
     1441 aaatgggcat ttgggaaaat gtctcaggtg ttctgacata gcattatcct tcggttccca 
     1501 attgctcaat tttccaccac aggcaaagca agccactctg tctccaggtc ctatgtagta 
     1561 aaagcctgct tttgccagat ctgttggcga cagaaaagtc aatggccatg tctgaaaagt 
     1621 aagtaatctg gcattttcgt tattcattgc acagtggtag gaacttctca tcaaggcaga 
     1681 aaaatcttga tttgctctgg agtttacagg atttgatgga gagtttgaat aagagccacg 
     1741 gaaatatcca ctgttttctg tacccggaag taatgagtgt gtggaatttg ttactgaaga 
     1801 aggaaaagta ggctgagagg tagcttccaa gttgttaacg gaatttagac tctgaacgaa 
     1861 tctgcagcta ggatacaact ttttatgctt ttcagtagga ctgtctcctc ttttccagtt 
     1921 atccagcatc aggccacaac agaagcattt gaccttgtca ttcacaccag tgtaatagaa 
     1981 accagcacga gcaagactcc tttctgagac aggaacccca gcaggaaaag tggaatacgt 
     2041 agacattcgg tacagttcac atgacaagtc gtatttcagt tcaaacgtgt tggcgctttt 
     2101 catcaaattt gataagaata tgctgttttc tactatgtcc atggtggcag gatccatgga 
     2161 gtctagaatt cccggggatc cacgcggaac cagaagtgcc ccggaggatg agattttctt 
     2221 aaagcggttg gctgctgaga cggctatgaa attctttttc catcgtcgct tcatatgtat 
     2281 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat 
     2341 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac 
     2401 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca 
     2461 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg 
     2521 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat 
     2581 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg 
     2641 agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc 
     2701 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta 
     2761 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 
     2821 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 
     2881 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 
     2941 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 
     3001 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 
     3061 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 
     3121 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 
     3181 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 
     3241 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 
     3301 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 
     3361 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 
     3421 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 
     3481 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 
     3541 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 
     3601 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 
     3661 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 
     3721 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 
     3781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 
     3841 aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc 
     3901 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat 
     3961 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt 
     4021 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg 
     4081 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga 
     4141 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt 
     4201 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg 
     4261 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct 
     4321 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac 
     4381 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc 
     4441 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac 
     4501 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat 
     4561 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg 
     4621 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg 
     4681 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact 
     4741 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg 
     4801 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa 
     4861 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg 
     4921 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt 
     4981 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac 
     5041 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc 
     5101 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa 
     5161 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc 
     5221 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat 
     5281 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 
     5341 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 
     5401 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 
     5461 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
     5521 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 
     5581 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     5641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     5701 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     5761 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     5821 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     5881 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     5941 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     6001 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     6061 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     6121 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     6181 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     6241 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     6301 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     6361 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     6421 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     6481 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     6541 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     6601 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     6661 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     6721 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     6781 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 
     6841 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     6901 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     6961 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     7021 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     7081 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 
     7141 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     7201 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     7261 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     7321 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     7381 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     7441 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     7501 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 
     7561 gcgtatcacg aggccctttc gtcttcaaga a 
//
