ID pCal-n-AK standard; circular DNA; ; 7591 BP. DE LMBP 5461. CC Accession number: LMBP 5461 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 17-JUL-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 2671..2748 FT /vntifkey="30" FT /label=lacI\promoter FT /note="E. coli repressor lacI promoter, incl. ribosome binding site" FT promoter complement(2321..2362) FT /vntifkey="30" FT /label=T7\promoter\with\lac\operator FT RBS complement(2275..2319) FT /vntifkey="32" FT /label=5'\UTR\of\T7\gene\10,\incl.\RBS FT CDS 2749..3831 FT /vntifkey="4" FT /label=lacI FT CDS 5143..5334 FT /vntifkey="4" FT /label=rop FT /note="modulation repressor coding sequence" FT rep_origin complement(5580..5982) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(6523..7383) FT /vntifkey="4" FT /label=ampicillin\resistance FT terminator complement(213..260) FT /vntifkey="43" FT /label=T7\terminator FT misc_marker complement(2176..2193) FT /vntifkey="22" FT /label=thrombin\recognition\site FT misc_marker complement(2194..2271) FT /vntifkey="22" FT /label=calmodulin-binding\peptide\tag FT CDS complement(331..2142) FT /vntifkey="4" FT /label=hAPI2 FT mutation complement(2137..2139) FT /vntifkey="62" FT /label=Kozak FT /note="AAC was replaced by GAC (N2D) creating Kozak consensus sequence GCCACCATGG" SQ Sequence 7591 BP; 1831 A; 1951 C; 1871 G; 1938 t; ttgtcatgtt tgacagctta tcttcgatta gctttaatgc ggtagtttat cacagttaaa 60 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300 cgggctttgt tagcagccgg atctaagctc tgaaagaaat gtacgaactg tacccttgat 360 tgtactccta caaataggac actttcttaa agaaggagca caatctttgc atactactag 420 atgaccacaa ggaataaaca ctatggacac ttctttgtcc atacacactt tacatgttct 480 ttcttcttgt agtctccgca attgttcttc cactggtaga tctgaaacat cttctgtggg 540 aatatatttt atgtcctgtt gcacaaataa atgctcatat aacacagctt cagcttcttg 600 cagagagttt ctgaatacag tggctgcaat atttcctttt actaaaatcg tatcaatcag 660 ttctcttgct tgtaaagacg tctgtgtctt ctgtttaata acatcatgtt cttgttcatt 720 aataattccg gcagttagta gactatccag gattggaatt acacaagtca aatgttgaaa 780 aagtgccatt ctattcttcc ggattaataa taaatcattt gattcttttt cctcagttgc 840 tctttctctc tcctcttccc ttatttcatc ttctgcattg agtaagtcta acacaagatc 900 attgactagt ctataattct ctccagttgc taggattttt ctctgaactg tctgttttac 960 caggcttcta ctaaagccca tttccacggc agcattaatc acaggagtat tcatcatgat 1020 tgcatcttct gaatggtctt ctccaggttc aaaatggata attgatgact ctgcattttc 1080 atctcctggg ctgtctgatg tggatagcag ctgttcaagt agatgagggt aactggcttg 1140 aacttgacgg atgaactcct gtcctttaat tcttatcaag tactcacacc ttggaaacca 1200 cttggcatgt tgaacccatg gatcatctcc agattcccaa cacctgagtc caccatcaca 1260 gcaaaagcat ttgacatcat cactgttacc cacataataa aaacccgcac ttgcaagctg 1320 ctcaggatta actagaacac tagagggcca gttaaagaat gttttaaagc gggctgcatg 1380 tgtctgcatg ctcagattag aaactgtgta tcttgaagtg tcttgaagct gattttctat 1440 aaatgggcat ttgggaaaat gtctcaggtg ttctgacata gcattatcct tcggttccca 1500 attgctcaat tttccaccac aggcaaagca agccactctg tctccaggtc ctatgtagta 1560 aaagcctgct tttgccagat ctgttggcga cagaaaagtc aatggccatg tctgaaaagt 1620 aagtaatctg gcattttcgt tattcattgc acagtggtag gaacttctca tcaaggcaga 1680 aaaatcttga tttgctctgg agtttacagg atttgatgga gagtttgaat aagagccacg 1740 gaaatatcca ctgttttctg tacccggaag taatgagtgt gtggaatttg ttactgaaga 1800 aggaaaagta ggctgagagg tagcttccaa gttgttaacg gaatttagac tctgaacgaa 1860 tctgcagcta ggatacaact ttttatgctt ttcagtagga ctgtctcctc ttttccagtt 1920 atccagcatc aggccacaac agaagcattt gaccttgtca ttcacaccag tgtaatagaa 1980 accagcacga gcaagactcc tttctgagac aggaacccca gcaggaaaag tggaatacgt 2040 agacattcgg tacagttcac atgacaagtc gtatttcagt tcaaacgtgt tggcgctttt 2100 catcaaattt gataagaata tgctgttttc tactatgtcc atggtggcag gatccatgga 2160 gtctagaatt cccggggatc cacgcggaac cagaagtgcc ccggaggatg agattttctt 2220 aaagcggttg gctgctgaga cggctatgaa attctttttc catcgtcgct tcatatgtat 2280 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat 2340 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac 2400 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca 2460 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg 2520 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat 2580 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg 2640 agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc 2700 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta 2760 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 2820 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 2880 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 2940 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 3000 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 3060 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 3120 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 3180 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 3240 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 3300 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 3360 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 3420 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 3480 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 3540 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 3600 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 3660 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 3720 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 3780 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 3840 aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc 3900 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat 3960 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt 4020 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg 4080 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga 4140 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt 4200 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg 4260 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct 4320 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac 4380 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc 4440 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac 4500 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat 4560 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg 4620 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg 4680 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact 4740 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg 4800 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa 4860 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg 4920 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt 4980 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac 5040 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc 5100 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa 5160 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc 5220 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat 5280 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 5340 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 5400 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 5460 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 5520 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 5580 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 5640 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 5700 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 5760 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 5820 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 5880 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 5940 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 6000 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 6060 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 6120 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 6180 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 6240 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 6300 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 6360 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6420 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 6480 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 6540 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 6600 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6660 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 6720 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 6780 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 6840 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 6900 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 6960 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7020 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7080 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 7140 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7200 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7260 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7320 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 7380 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 7440 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 7500 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 7560 gcgtatcacg aggccctttc gtcttcaaga a 7591 //