ID pCal-c-AK standard; circular DNA; ; 7575 BP. DE LMBP 5463. CC Accession number: LMBP 5463 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 17-JUL-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 2655..2732 FT /vntifkey="30" FT /label=lacI\promoter FT /note="E. coli repressor lacI promoter, incl. ribosome binding site" FT RBS complement(2261..2303) FT /vntifkey="32" FT /label=5'\UTR\of\T7\gene\10,\incl.\RBS FT promoter complement(2305..2346) FT /vntifkey="30" FT /label=T7\promoter\with\lac\operator FT CDS 2733..3815 FT /vntifkey="4" FT /label=lacI FT CDS 5127..5318 FT /vntifkey="4" FT /label=rop FT /note="modulation repressor coding sequence" FT rep_origin complement(5564..5966) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(6507..7367) FT /vntifkey="4" FT /label=ampicillin\resistance FT terminator complement(213..260) FT /vntifkey="43" FT /label=T7\terminator FT misc_marker complement(325..402) FT /vntifkey="22" FT /label=calmodulin-binding\peptide\tag FT misc_marker complement(409..426) FT /vntifkey="22" FT /label=thrombin\recognition\site FT CDS complement(433..2244) FT /vntifkey="4" FT /label=hAPI2 FT mutation complement(2239..2241) FT /vntifkey="62" FT /label=Kozak FT /note="AAC was replaced by GAC (N2D) creating Kozak consensus sequence GCCACCATGG" SQ Sequence 7575 BP; 1828 A; 1948 C; 1865 G; 1934 t; ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300 cgggctttgt tagcagccgg atcaaagtgc cccggaggat gagattttct taaagcggtt 360 ggctgctgag acggctatga aattcttttt ccatcgtcgc ttggtaccat tcccacgtgg 420 atacatggat cctgaaagaa atgtacgaac tgtacccttg attgtactcc tacaaatagg 480 acactttctt aaagaaggag cacaatcttt gcatactact agatgaccac aaggaataaa 540 cactatggac acttctttgt ccatacacac tttacatgtt ctttcttctt gtagtctccg 600 caattgttct tccactggta gatctgaaac atcttctgtg ggaatatatt ttatgtcctg 660 ttgcacaaat aaatgctcat ataacacagc ttcagcttct tgcagagagt ttctgaatac 720 agtggctgca atatttcctt ttactaaaat cgtatcaatc agttctcttg cttgtaaaga 780 cgtctgtgtc ttctgtttaa taacatcatg ttcttgttca ttaataattc cggcagttag 840 tagactatcc aggattggaa ttacacaagt caaatgttga aaaagtgcca ttctattctt 900 ccggattaat aataaatcat ttgattcttt ttcctcagtt gctctttctc tctcctcttc 960 ccttatttca tcttctgcat tgagtaagtc taacacaaga tcattgacta gtctataatt 1020 ctctccagtt gctaggattt ttctctgaac tgtctgtttt accaggcttc tactaaagcc 1080 catttccacg gcagcattaa tcacaggagt attcatcatg attgcatctt ctgaatggtc 1140 ttctccaggt tcaaaatgga taattgatga ctctgcattt tcatctcctg ggctgtctga 1200 tgtggatagc agctgttcaa gtagatgagg gtaactggct tgaacttgac ggatgaactc 1260 ctgtccttta attcttatca agtactcaca ccttggaaac cacttggcat gttgaaccca 1320 tggatcatct ccagattccc aacacctgag tccaccatca cagcaaaagc atttgacatc 1380 atcactgtta cccacataat aaaaacccgc acttgcaagc tgctcaggat taactagaac 1440 actagagggc cagttaaaga atgttttaaa gcgggctgca tgtgtctgca tgctcagatt 1500 agaaactgtg tatcttgaag tgtcttgaag ctgattttct ataaatgggc atttgggaaa 1560 atgtctcagg tgttctgaca tagcattatc cttcggttcc caattgctca attttccacc 1620 acaggcaaag caagccactc tgtctccagg tcctatgtag taaaagcctg cttttgccag 1680 atctgttggc gacagaaaag tcaatggcca tgtctgaaaa gtaagtaatc tggcattttc 1740 gttattcatt gcacagtggt aggaacttct catcaaggca gaaaaatctt gatttgctct 1800 ggagtttaca ggatttgatg gagagtttga ataagagcca cggaaatatc cactgttttc 1860 tgtacccgga agtaatgagt gtgtggaatt tgttactgaa gaaggaaaag taggctgaga 1920 ggtagcttcc aagttgttaa cggaatttag actctgaacg aatctgcagc taggatacaa 1980 ctttttatgc ttttcagtag gactgtctcc tcttttccag ttatccagca tcaggccaca 2040 acagaagcat ttgaccttgt cattcacacc agtgtaatag aaaccagcac gagcaagact 2100 cctttctgag acaggaaccc cagcaggaaa agtggaatac gtagacattc ggtacagttc 2160 acatgacaag tcgtatttca gttcaaacgt gttggcgctt ttcatcaaat ttgataagaa 2220 tatgctgttt tctactatgt ccatggtggc aggatccatg gtatatctcc ttcttaaagt 2280 taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc 2340 gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat 2400 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 2460 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 2520 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 2580 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 2640 gcgtcgagat cccggacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc 2700 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg 2760 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg 2820 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca 2880 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca 2940 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac 3000 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg 3060 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg 3120 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg 3180 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg 3240 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa 3300 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa 3360 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca 3420 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg 3480 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg 3540 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca 3600 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg 3660 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc 3720 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 3780 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag 3840 ctcactcatt aggcaccggg atctcgaccg atgcccttga gagccttcaa cccagtcagc 3900 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc 3960 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt 4020 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc 4080 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc 4140 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg 4200 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag 4260 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg 4320 gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc 4380 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc 4440 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc 4500 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa 4560 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca 4620 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc 4680 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat 4740 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa 4800 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc 4860 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac 4920 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc 4980 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat 5040 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca 5100 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg 5160 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg 5220 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct 5280 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 5340 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 5400 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 5460 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt 5520 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 5580 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5640 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5700 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5760 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5820 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 5880 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 5940 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 6000 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 6060 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 6120 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 6180 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 6240 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 6300 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 6360 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 6420 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 6480 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 6540 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 6600 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 6660 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6720 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6780 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca 6840 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6900 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6960 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 7020 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 7080 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 7140 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 7200 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 7260 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 7320 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 7380 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 7440 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 7500 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 7560 tttcgtcttc aagaa 7575 //