LOCUS       pCal-c-MK               8235 bp    DNA     circular     17-JUL-2012
DEFINITION  LMBP 5464.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5464
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-JUL-2012
            
            
COMMENT     VNTDATE|489320866|
COMMENT     VNTNAME|pCal-c-MK|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCal-c-MK" 0 8235 
COMMENT       (CDBMol 0 0 1 5230 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AatII" "Alw44I" "BamHI" "BglII" "HincII" "KpnI" "NcoI" 
COMMENT         "PaeI" "PdmI" "PstI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 2921 2963 1 8235 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 8163 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 5 1953 0 3724 0 6162 0 
COMMENT            6662 0 7908 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 428 0 2913 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BglII" "agatct" 2 0 1 3022 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HincII" "gtyrac" 4 0 2 4250 0 7782 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 408 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 630 0 2902 0 2917 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PaeI" "gcatgc" 6 0 1 3219 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "PdmI" "gaannnnttc" 6 0 4 1638 0 2552 0 5906 0 
COMMENT            7840 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 686 0 2577 0 7486 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "lacI promoter" 30 0 0 3315 3392 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3315..3392") 
COMMENT        #11=(CFSignal (CObList) "T7 promoter with lac operator" 30 0 1 2965 3006 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "complement(2963..3006)") 
COMMENT        #12=(CFSignal (CObList) "lacI" 4 0 0 3393 4475 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3393..4475") 
COMMENT        #13=(CFSignal (CObList) "rop" 4 0 0 5787 5978 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5787..5978") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 6224 6626 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6224..6626)") 
COMMENT        #15=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7167 8027 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(7167..8027)") 
COMMENT        #16=(CFSignal (CObList) "T7 terminator" 43 0 1 213 260 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(213..260)") 
COMMENT        #17=(CFSignal (CObList) "calmodulin-binding peptide tag" 22 0 1 325 402 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "complement(325..402)") 
COMMENT        #18=(CFSignal (CObList) "thrombin recognition site" 22 0 1 409 426 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(409..426)") 
COMMENT        #19=(CFSignal (CObList) "hMLT" 4 0 1 433 2904 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(433..2904)") 
COMMENT        #20=(CFSignal (CObList) "PCR artefact" 62 0 1 955 957 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(955..957)") 
COMMENT        #21=(CFSignal (CObList) "stability creator" 62 0 1 1417 1419 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1417..1419)") 
COMMENT        #22=(CFSignal (CObList) "Kozak" 62 0 1 2899 2901 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2899..2901)") 
COMMENT        #23=(CFSignal (CObList) "5' UTR of T7 gene 10, incl. RBS" 32 0 1 2921 
COMMENT             2963 0 (CStringList) (CStringList) 1 1 1 1 "complement(2921..2963)")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT        #25=(CFSignal (CObList) "Last modified: 17-JUL-2012" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCal-c-MK'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 07/31/08 10:27" 1) 
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COMMENT                          "Last Modified: 07/17/12 12:46" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8235 bp" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 12-SEP-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                 "thrombin recognition site"))) 
COMMENT                          "Misc. Marker (2 total)") 
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COMMENT                                   #84=(CLinePar 
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COMMENT                                        "E. coli repressor lacI promoter, incl. ribosome binding site" 
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COMMENT                                        "   3315..3392" 1))) "lacI promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                                           "silent mutation; ACA was replaced by ACG" 
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COMMENT                                           "silent mutation; wt: GCA ('GCA'GAAG recombines with the same heptamer in hAPI2)" 
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FEATURES             Location/Qualifiers
     promoter        3315..3392
                     /vntifkey="30"
                     /label=lacI\promoter
                     /note="E. coli repressor lacI promoter, incl. ribosome binding site"
     RBS             complement(2921..2963)
                     /vntifkey="32"
                     /label=5'\UTR\of\T7\gene\10,\incl.\RBS
     promoter        complement(2965..3006)
                     /vntifkey="30"
                     /label=T7\promoter\with\lac\operator
     CDS             3393..4475
                     /vntifkey="4"
                     /label=lacI
     CDS             5787..5978
                     /vntifkey="4"
                     /label=rop
                     /note="modulation repressor coding sequence"
     rep_origin      complement(6224..6626)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(7167..8027)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      complement(213..260)
                     /vntifkey="43"
                     /label=T7\terminator
     misc_marker     complement(325..402)
                     /vntifkey="22"
                     /label=calmodulin-binding\peptide\tag
     misc_marker     complement(409..426)
                     /vntifkey="22"
                     /label=thrombin\recognition\site
     CDS             complement(433..2904)
                     /vntifkey="4"
                     /label=hMLT
     mutation        complement(955..957)
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation; ACA was replaced by ACG"
     mutation        complement(1417..1419)
                     /vntifkey="62"
                     /label=stability\creator
                     /note="silent mutation; wt: GCA ('GCA'GAAG recombines with the same heptamer in hAPI2)"
     mutation        complement(2899..2901)
                     /vntifkey="62"
                     /label=Kozak
                     /note="TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG"
BASE COUNT     1982 a      2152 c      2025 g      2076 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 
      241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 
      301 cgggctttgt tagcagccgg atcaaagtgc cccggaggat gagattttct taaagcggtt 
      361 ggctgctgag acggctatga aattcttttt ccatcgtcgc ttggtaccat tcccacgtgg 
      421 atacatggat cctttttcag aaattctgag cctgtcagag aaactaaatg gtatttcatc 
      481 agttgtctct actggcacat tacttctact aaaatgacat gaagcatggt gagcgaaact 
      541 tgaaataaat gcatctggag tccggctgca atgacagcta tctgctggtg taacattact 
      601 tggattacca ggatgtgaat ggtaaacacc atggaatggg tcttgcaatg agtgataatg 
      661 ccctgctcct cctgaggtgg ctgcagaact ctggtaagga ccattagaca taagacaagt 
      721 ttgaaagcaa gtcttccttc ccaaacctcg atgcatgtct aatttagcaa tgagaggttt 
      781 cccaacattc acttcctgct tgtcctctac agtatcttcc aatcctgagt actgatatga 
      841 taaacatact gtgaagacta gatgttcctt taatttttgc agtgaactga gtctggtata 
      901 gaggcaatgc ttgggaagat cctttgatac caagtagctg ccagtttctt caggcgtgcc 
      961 tttatttgca tcttttggat caatatctag atcaagtgga aaatcagtaa cgtaggcatc 
     1021 acacattatt atctccggtg gtttgtaaac tatacttgta tagatgatca tgacattgga 
     1081 aaactcagct gcaaatccta attgaatctg aacaccacag tcaaacttaa gacacatact 
     1141 ttctggaagt tcatgagcct tggcccactg tagattccgc acaagagatt cagcagaata 
     1201 ttctgttccc tgtattggat cagtaagtgc tctcttctca gataaactac ttcgaatctc 
     1261 tagagcctgt ttgcctttgg taaggtgaca cttacccata tcttctgcaa cttcatccag 
     1321 taacacagtg attttcttat cttctaataa tctgtctttt aaaaatttca taaagattcc 
     1381 atttgccaat ccagaatgct ggatttcaaa agcttcagct ccttgacacg tggcatatcc 
     1441 aaacacaata ttggcggtga cttttagtgc atccaagatt ggaatggtat catcgtagtc 
     1501 atttcttttc ctacacatat ccaataagaa cacattaagt ccagtttctt tttcttgcat 
     1561 caatttcagt atattttgta cacacagaca attttcagac ctatatggat ttggagcatc 
     1621 aacggggacc atgaagctgt tcccaaaatt ttcataacca tgtcctgcat aatataataa 
     1681 cccatatact cccttgtcta aaaggagtaa aaactcatcc acagcattac gcatctcata 
     1741 ttcagtaaga tccaacagtg aaaccacttt gaagtccagc tgtctcagta agttagtcaa 
     1801 ttcgtacaca tccaccaaag gagctttgag cttggggtgc tcccggtaat tcatatttcc 
     1861 tatcaaaagg gcaaccttgt ccttcgccaa aggctggtca gttgtttgct ctttattatc 
     1921 aggatgacca agattattta attcatcttc agtgcactcc actgcctcat ctgttcttcc 
     1981 tatgatgatt tctaccttct tgctatcttg actgtctcga tcattatata catgacacca 
     2041 gtaggttcct tggtgttcca aatccacata aggcaccatg tatagctttt tggtctcatg 
     2101 tgttaatggt aattcatttt tgaaccactg gtagtgagga atagggcttc caacagcaac 
     2161 acactgtaaa accaatgtgc tgcctggcat cagcttttgg gaagttggtt caacacagat 
     2221 ttgcaacttg gattcagaga cgccatcaac acttctctgg aagctctctg ggatgtcgca 
     2281 aacatccagc tgtgaccact ggctgaattc aaaggtgaaa ttgttattaa ctcgacagac 
     2341 ataaaagcct gcatctttta catgcactgc attaaaaata agctctgatg tatttccatt 
     2401 tggaatctct ttattcattt tgaaccactg atattgaaca aaaggatgtc cagttgcccg 
     2461 gcaacacagt ttcacaaact gtccagccaa gactgccttt gactctgggt ttacagtaat 
     2521 ctttattcct ggggggctga gaagctgaag aacttcagtg tgttccatag cctgcaggaa 
     2581 atcactcaat tctgtgactg tgcaaccttt ttcacccatt aacttcagca gacacaggct 
     2641 ggggcttcct tcaggctcca gtaccttaag agaacactgc tccaggtcta ggcaactgag 
     2701 gcggaggcgc ccgcgactcc ccgccagctc cgccagtctc ctccagcccc ggccctcggg 
     2761 cgcctgatcc aggagctcgc tgagcctccg cagcagcggc tcccgcaggc ggttgagggt 
     2821 cgcgccggcc ggaggggcga gcagcggccc cgtgggggcg gccgagggcg gcagggcctg 
     2881 tagcgggtcc cccaacagcg ccatggtggc aggatccatg gtatatctcc ttcttaaagt 
     2941 taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc 
     3001 gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat 
     3061 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 
     3121 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 
     3181 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 
     3241 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 
     3301 gcgtcgagat cccggacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc 
     3361 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg 
     3421 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg 
     3481 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca 
     3541 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca 
     3601 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac 
     3661 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg 
     3721 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg 
     3781 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg 
     3841 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg 
     3901 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa 
     3961 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa 
     4021 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca 
     4081 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg 
     4141 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg 
     4201 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca 
     4261 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg 
     4321 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc 
     4381 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 
     4441 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag 
     4501 ctcactcatt aggcaccggg atctcgaccg atgcccttga gagccttcaa cccagtcagc 
     4561 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc 
     4621 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt 
     4681 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc 
     4741 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc 
     4801 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg 
     4861 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag 
     4921 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg 
     4981 gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc 
     5041 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc 
     5101 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc 
     5161 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa 
     5221 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca 
     5281 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc 
     5341 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat 
     5401 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa 
     5461 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc 
     5521 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac 
     5581 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc 
     5641 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat 
     5701 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca 
     5761 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg 
     5821 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg 
     5881 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct 
     5941 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 
     6001 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 
     6061 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 
     6121 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt 
     6181 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 
     6241 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     6301 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
     6361 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     6421 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     6481 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     6541 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     6601 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     6661 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     6721 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     6781 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
     6841 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     6901 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
     6961 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
     7021 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
     7081 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
     7141 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
     7201 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
     7261 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
     7321 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
     7381 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
     7441 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca 
     7501 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
     7561 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
     7621 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
     7681 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
     7741 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 
     7801 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
     7861 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
     7921 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
     7981 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
     8041 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
     8101 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 
     8161 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 
     8221 tttcgtcttc aagaa 
//
