LOCUS       pCAGGS-Flag-IKKalpha        7082 bp    DNA     circular     18-JAN-2008
DEFINITION  LMBP 5483.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5483
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 18-JAN-2008
            
COMMENT     VNTDATE|442945158|
COMMENT     VNTNAME|pCAGGS-Flag-IKKalpha|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-Flag-IKKalpha" 0 7082 
COMMENT       (CDBMol 0 0 1 2433 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "Alw44I" "ApaI" "KpnI" "NcoI" "NheI" "PvuI" "PvuII" "XbaI" 
COMMENT         "XhoI" "NotI" "EcoRI" "FspI" "Eco147I") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 6066 6065 1 7082 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 4 1334 0 1814 0 3850 0 
COMMENT            5096 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 3 84 0 5860 0 6219 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 42 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 42 0 669 0 855 0 3111 0 
COMMENT            5731 0 0 "") #4=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 4799 0 5354 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PvuII" "cagctg" 4 0 1 1309 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XbaI" "tctaga" 2 0 3 1159 0 2053 0 6985 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 5 0 2282 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 2288 0 7081 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 4651 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "Eco147I" "aggcct" 4 0 3 269 0 700 0 3203 0 0 
COMMENT             "")) 
COMMENT       (CObList 
COMMENT        #13=(CFSignal (CObList) "intron AG promoter" 15 0 0 6107 7031 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6107..7031") 
COMMENT        #14=(CFSignal (CObList) "AG promoter" 29 0 0 5738 7079 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5738..7079") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5355 5732 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5355..5732") 
COMMENT        #16=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "13..36") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 3413 3814 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3413..3814)") 
COMMENT        #18=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3224 3298 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3224..3298)") 
COMMENT        #19=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3224 3298 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3224..3298") 
COMMENT        #20=(CFSignal (CObList) "SV40 ori" 33 0 0 3118 3193 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3118..3193") 
COMMENT        #21=(CFSignal (CObList) "lac promoter" 30 0 1 2894 2976 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2894..2976)") 
COMMENT        #22=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 2460 2816 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2460..2816") 
COMMENT        #23=(CFSignal (CObList) "\337-globin polyA" 43 0 0 2368 2459 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2368..2459") 
COMMENT        #24=(CFSignal (CObList) "ampicillin" 4 0 1 4355 5215 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4355..5215)") 
COMMENT        #25=(CFSignal (CObList) "hIKKalpha" 4 0 0 43 2280 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #26=(CFSignal (CObList) "LMBP 5483" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #27=(CFSignal (CObList) "Last modified: 18-JAN-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    "pCAGGS-Flag-IKKalpha" 2) 5 "" 0 4) 
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COMMENT                    (CObjectList 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-Flag-IKKalpha'" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5483" 
COMMENT                          1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 04/06/07 04:19" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/18/08 09:57" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7082 bp" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #39=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 25-SEP-2007" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  CHUK" 1))) "Standard Fields") 
COMMENT              #43=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #44=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 5483") 
COMMENT                     #47=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #48=(CLinePar (CParagraph 0 (15 #27# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 18-JAN-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #49=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #50=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 43 (3 #25# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 43    End: 2280" 1))) 
COMMENT                                 "hIKKalpha") 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4355 (3 #24# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4355  End: 5215 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4355..5215)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
COMMENT                     #57=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 6107  End: 7031" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   6107..7031" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
COMMENT                     #62=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 2460  End: 2816" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' flanking region of the rabbit \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2460..2816" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #68=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #69=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 13    End: 36  " 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   13..36" 1))) "FLAG epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5355  End: 5732" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5355..5732" 1))) "hCMV-IE enhancer") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5738 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5738  End: 7079" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5738..7079" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #82=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2894  End: 2976 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2894..2976)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #87=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3118 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3118  End: 3193" 1) 
COMMENT                                   #90=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3118..3193" 1))) "SV40 ori") 
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COMMENT                                        "Start: 3413  End: 3814 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3413..3814)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #96=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2368  End: 2459" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' UTR of the rabbit \337-globin gene, incl. polyA" 
COMMENT                                        1) 
COMMENT                                   #100=(CLinePar 
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COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
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COMMENT                                         "   2368..2459" 1))) 
COMMENT                                 "\337-globin polyA") 
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COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #105=(CLinePar 
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COMMENT                                          "   complement(3224..3298)" 1))) 
COMMENT                                  "SV40 polyA late") 
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COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   3224..3298" 1))) 
COMMENT                                  "SV40 polyA early"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                     (CObjectList 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #112=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" 
COMMENT                            "CGCCGGCG") 
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COMMENT                            (CFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 42 " 1))))) "KpnI: 1 site") 5 
COMMENT                            1 "GGTACC" "CCATGG") 
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COMMENT                                            " N1: 1309 " 1))))) "PvuII: 1 site") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
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COMMENT                                            " N1: 4651 " 1))))) "FspI: 1 site") 
COMMENT                            3 3 "TGCGCA" "ACGCGT") 
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COMMENT                                            " N1: 4799 " 1) 
COMMENT                                      #125=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5354 " 1))))) "PvuI: 2 sites") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
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COMMENT                                            " N1: 5 " 1) 
COMMENT                                      #129=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2282 " 1))))) "XhoI: 2 sites") 
COMMENT                            1 5 "CTCGAG" "GAGCTC") 
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COMMENT                                            " N1: 2288 " 1) 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 7081 " 1))))) "EcoRI: 2 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                                            " N1: 84 " 1) 
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COMMENT                                            " N2: 5860 " 1) 
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FEATURES             Location/Qualifiers
     intron          6107..7031
                     /vntifkey="15"
                     /label=intron\AG\promoter
     promoter        5738..7079
                     /vntifkey="29"
                     /label=AG\promoter
     promoter        5355..5732
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
     rep_origin      complement(3413..3814)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(3224..3298)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      3224..3298
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      3118..3193
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(2894..2976)
                     /vntifkey="30"
                     /label=lac\promoter
     misc_feature    2460..2816
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      2368..2459
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     CDS             complement(4355..5215)
                     /vntifkey="4"
                     /label=ampicillin
     CDS             43..2280
                     /vntifkey="4"
                     /label=hIKKalpha
BASE COUNT     1705 a      1690 c      1886 g      1801 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg 
       61 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc 
      121 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt 
      181 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag 
      241 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg 
      301 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg 
      361 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt 
      421 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa 
      481 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg 
      541 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag 
      601 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc 
      661 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag 
      721 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa 
      781 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt 
      841 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag 
      901 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt aatggatcac 
      961 attttgaatt tgaagatagt acacatccta aatatgactt ctgcaaagat aatttctttt 
     1021 ctgttaccac ctgatgaaag tcttcattca ctacagtctc gtattgagcg tgaaactgga 
     1081 ataaatactg gttctcaaga acttctttca gagacaggaa tttctctgga tcctcggaaa 
     1141 ccagcctctc aatgtgttct agatggagtt agaggctgtg atagctatat ggtttatttg 
     1201 tttgataaaa gtaaaactgt atatgaaggg ccatttgctt ccagaagttt atctgattgt 
     1261 gtaaattata ttgtacagga cagcaaaata cagcttccaa ttatacagct gcgtaaagtg 
     1321 tgggctgaag cagtgcacta tgtgtctgga ctaaaagaag actatagcag gctctttcag 
     1381 ggacaaaggg cagcaatgtt aagtcttctt agatataatg ctaacttaac aaaaatgaag 
     1441 aacactttga tctcagcatc acaacaactg aaagctaaat tggagttttt tcacaaaagc 
     1501 attcagcttg acttggagag atacagcgag cagatgacgt atgggatatc ttcagaaaaa 
     1561 atgctaaaag catggaaaga aatggaagaa aaggccatcc actatgctga ggttggtgtc 
     1621 attggatacc tggaggatca gattatgtct ttgcatgctg aaatcatgga gctacagaag 
     1681 agcccctatg gaagacgtca gggagacttg atggaatctc tggaacagcg tgccattgat 
     1741 ctatataagc agttaaaaca cagaccttca gatcactcct acagtgacag cacagagatg 
     1801 gtgaaaatca ttgtgcacac tgtgcagagt caggaccgtg tgctcaagga gctgtttggt 
     1861 catttgagca agttgttggg ctgtaagcag aagattattg atctactccc taaggtggaa 
     1921 gtggccctca gtaatatcaa agaagctgac aatactgtca tgttcatgca gggaaaaagg 
     1981 cagaaagaaa tatggcatct ccttaaaatt gcctgtacac agagttctgc ccggtccctt 
     2041 gtaggatcca gtctagaagg tgcagtaacc cctcagacat cagcatggct gcccccgact 
     2101 tcagcagaac atgatcattc tctgtcatgt gtggtaactc ctcaagatgg ggagacttca 
     2161 gcacaaatga tagaagaaaa tttgaactgc cttggccatt taagcactat tattcatgag 
     2221 gcaaatgagg aacagggcaa tagtatgatg aatcttgatt ggagttggtt aacagaatga 
     2281 ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc 
     2341 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac 
     2401 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca 
     2461 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat 
     2521 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct 
     2581 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc 
     2641 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt 
     2701 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt 
     2761 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc 
     2821 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 
     2881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc 
     2941 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     3001 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa 
     3061 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     3121 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 
     3181 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc 
     3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg 
     3361 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     3421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     3481 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt 
     3541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     3601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     3661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     3721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     3781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     3841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     3901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     3961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     4021 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     4081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     4141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     4201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     4261 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     4321 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     4381 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     4441 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     4501 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     4561 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     4621 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     4681 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     4741 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     4801 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     4861 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     4921 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     4981 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     5041 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     5101 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     5161 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     5221 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     5281 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     5341 cacctgggtc gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc 
     5401 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 
     5461 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 
     5521 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 
     5581 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 
     5641 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 
     5701 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt 
     5761 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt 
     5821 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg 
     5881 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc 
     5941 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag 
     6001 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc 
     6061 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga 
     6121 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct 
     6181 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc 
     6241 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg 
     6301 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg 
     6361 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg 
     6421 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca 
     6481 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct 
     6541 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt 
     6601 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc 
     6661 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat 
     6721 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga 
     6781 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg 
     6841 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc 
     6901 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg 
     6961 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct 
     7021 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag 
     7081 aa 
//
