LOCUS       pCAGGS-Flag-IKKalpha-K        5756 bp    DNA     circular     28-JAN-2008
DEFINITION  LMBP 5485.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5485
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-JAN-2008
            
COMMENT     VNTDATE|445964865|
COMMENT     VNTNAME|pCAGGS-Flag-IKKalpha-K|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-Flag-IKKalpha-K" 0 5756 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "Alw44I" "ApaI" "EcoRI" "KpnI" "NcoI" "NheI" "NotI" "PvuI" 
COMMENT         "XbaI" "XhoI" "FspI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5756 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 2 2524 0 3770 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "ApaI" "gggccc" 6 0 3 84 0 4534 0 4893 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 962 0 5755 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 42 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 42 0 669 0 855 0 1785 0 
COMMENT            4405 0 0 "") #8=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 3473 0 4028 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 5659 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 5 0 956 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3325 0 0 "")) 
COMMENT       (CObList 
COMMENT        #14=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "13..36") 
COMMENT        #15=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1042 1133 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1042..1133") 
COMMENT        #16=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1134 1490 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1134..1490") 
COMMENT        #17=(CFSignal (CObList) "lac promoter" 30 0 1 1568 1650 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1568..1650)") 
COMMENT        #18=(CFSignal (CObList) "SV40 ori" 33 0 0 1792 1867 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1792..1867") 
COMMENT        #19=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1898 1972 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1898..1972") 
COMMENT        #20=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1898 1972 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1898..1972)") 
COMMENT        #21=(CFSignal (CObList) "pMB1 ori" 33 0 1 2087 2488 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2087..2488)") 
COMMENT        #22=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4029 4406 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4029..4406") 
COMMENT        #23=(CFSignal (CObList) "AG promoter" 29 0 0 4412 5753 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4412..5753") 
COMMENT        #24=(CFSignal (CObList) "intron AG promoter" 15 0 0 4781 5705 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4781..5705") 
COMMENT        #25=(CFSignal (CObList) "hIKKalpha-K" 4 0 0 43 954 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "43..951") 
COMMENT        #26=(CFSignal (CObList) "ampicillin" 4 0 1 3029 3889 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3029..3889)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #27=(CFSignal (CObList) "LMBP 5485" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #28=(CFSignal (CObList) "Last modified: 28-JAN-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #29=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #30=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pCAGGS-Flag-IKKalpha-K" 2) 5 "" 0 4) 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-Flag-IKKalpha-K'" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5485." 
COMMENT                          1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 05/09/07 03:07" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/28/08 11:05" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5756 bp" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 10-OCT-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #43=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #44=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CLinePar (CParagraph 0 (15 #27# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5485") 
COMMENT                     #47=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #48=(CLinePar (CParagraph 0 (15 #28# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 28-JAN-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #49=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #50=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 43 (3 #25# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 43    End: 954 " 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   43..951" 1))) "hIKKalpha-K") 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3029 (3 #26# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3029  End: 3889 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3029..3889)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
COMMENT                     #60=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #61=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4781 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4781  End: 5705" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Chicken \337-actin/rabbit \337-globin hybrid intron" 
COMMENT                                        1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4781..5705" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
COMMENT                     #66=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1134 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1134  End: 1490" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' flanking region of the rabbit \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1134..1490" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #72=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #73=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 13 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 13    End: 36  " 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "FLAG epitope tag" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   13..36" 1))) "FLAG epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #78=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4029 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4029  End: 4406" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Human cytomegalovirus immediate early enhancer" 
COMMENT                                        1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4029..4406" 1))) "hCMV-IE enhancer") 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4412 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4412  End: 5753" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4412..5753" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #89=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #90=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1568 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1568  End: 1650 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli lac operon promoter" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1568..1650)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #95=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #96=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1792 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1792  End: 1867" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "bidirectional origin of SV40" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   1792..1867" 1))) "SV40 ori") 
COMMENT                            #101=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 2087 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 2087  End: 2488 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "E. coli plasmid pMB1 origin" 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(2087..2488)" 1))) 
COMMENT                                  "pMB1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #106=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #107=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1042 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1042  End: 1133" 1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "3' UTR of the rabbit \337-globin gene, incl. polyA" 
COMMENT                                           1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1042..1133" 1))) 
COMMENT                                   "\337-globin polyA") 
COMMENT                             #112=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1898 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1898  End: 1972" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "polyA signal of the SV40 early region" 
COMMENT                                           1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1898..1972" 1))) 
COMMENT                                   "SV40 polyA early") 
COMMENT                             #117=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1898 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1898  End: 1972 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "polyA signal of the SV40 late region" 
COMMENT                                           1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
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FEATURES             Location/Qualifiers
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
                     /note="FLAG epitope tag"
     terminator      1042..1133
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    1134..1490
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(1568..1650)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      1792..1867
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      1898..1972
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(1898..1972)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2087..2488)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="E. coli plasmid pMB1 origin"
     promoter        4029..4406
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="Human cytomegalovirus immediate early enhancer"
     promoter        4412..5753
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="Chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          4781..5705
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="Chicken ß-actin/rabbit ß-globin hybrid intron"
     CDS             43..954
                     /vntifkey="4"
                     /label=hIKKalpha-K
     CDS             complement(3029..3889)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1283 a      1447 c      1583 g      1443 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg 
       61 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc 
      121 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt 
      181 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag 
      241 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg 
      301 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg 
      361 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt 
      421 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa 
      481 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg 
      541 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag 
      601 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc 
      661 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag 
      721 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa 
      781 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt 
      841 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag 
      901 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt atgactcgag 
      961 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc 
     1021 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg 
     1081 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg 
     1141 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa 
     1201 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg 
     1261 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc 
     1321 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta 
     1381 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc 
     1441 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg 
     1501 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 
     1561 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga 
     1621 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     1681 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     1741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     1801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     1861 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa 
     1921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     1981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat 
     2041 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 
     2101 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     2161 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa 
     2221 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     2281 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     2341 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     2401 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     2461 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     2521 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     2581 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     2641 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     2701 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     2761 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     2821 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     2881 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     2941 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     3001 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     3061 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     3121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     3181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     3241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     3301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     3361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     3421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     3481 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     3541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     3601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     3661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     3721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     3781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     3841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     3901 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     3961 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     4021 ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 
     4081 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 
     4141 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 
     4201 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 
     4261 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 
     4321 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 
     4381 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct 
     4441 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 
     4501 tgcagcgatg ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga 
     4561 ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg 
     4621 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg 
     4681 gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg 
     4741 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc 
     4801 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct 
     4861 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg 
     4921 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg 
     4981 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc 
     5041 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg 
     5101 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc 
     5161 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac 
     5221 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg 
     5281 cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga 
     5341 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg 
     5401 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc 
     5461 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg 
     5521 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg 
     5581 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct 
     5641 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc 
     5701 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa 
//
