ID pCAGGS-Flag-IKKalpha-K standard; circular DNA; ; 5756 BP. DE LMBP 5485. CC Accession number: LMBP 5485 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 28-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT /note="FLAG epitope tag" FT terminator 1042..1133 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 1134..1490 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(1568..1650) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 1792..1867 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 1898..1972 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(1898..1972) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(2087..2488) FT /vntifkey="33" FT /label=pMB1\ori FT /note="E. coli plasmid pMB1 origin" FT promoter 4029..4406 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="Human cytomegalovirus immediate early enhancer" FT promoter 4412..5753 FT /vntifkey="29" FT /label=AG\promoter FT /note="Chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 4781..5705 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="Chicken ß-actin/rabbit ß-globin hybrid intron" FT CDS 43..954 FT /vntifkey="4" FT /label=hIKKalpha-K FT CDS complement(3029..3889) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 5756 BP; 1283 A; 1447 C; 1583 G; 1443 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg 60 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc 120 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt 180 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag 240 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg 300 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg 360 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt 420 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa 480 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg 540 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag 600 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc 660 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag 720 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa 780 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt 840 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag 900 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt atgactcgag 960 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc 1020 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg 1080 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg 1140 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa 1200 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg 1260 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc 1320 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta 1380 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc 1440 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg 1500 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 1560 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga 1620 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 1680 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 1740 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 1800 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 1860 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa 1920 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 1980 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat 2040 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 2100 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 2160 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa 2220 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 2280 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 2340 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 2400 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 2460 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 2520 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 2580 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 2640 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 2700 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 2760 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 2820 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 2880 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 2940 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 3000 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 3060 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 3120 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 3180 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 3240 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 3300 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 3360 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 3420 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 3480 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 3540 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 3600 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 3660 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 3720 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 3780 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 3840 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 3900 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 3960 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 4020 ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 4080 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 4140 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 4200 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 4260 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 4320 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 4380 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct 4440 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 4500 tgcagcgatg ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga 4560 ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg 4620 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg 4680 gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg 4740 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc 4800 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct 4860 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg 4920 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg 4980 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc 5040 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg 5100 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc 5160 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac 5220 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg 5280 cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga 5340 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg 5400 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc 5460 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg 5520 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg 5580 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct 5640 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc 5700 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa 5756 //