LOCUS       pCAGGS-Flag-IKKalpha-KL        6302 bp    DNA     circular     28-JAN-2008
DEFINITION  LMBP 5486.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5486
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-JAN-2008
            
COMMENT     VNTDATE|446031121|
COMMENT     VNTNAME|pCAGGS-Flag-IKKalpha-KL|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-Flag-IKKalpha-KL" 0 6302 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "Alw44I" "ApaI" "EcoRI" "KpnI" "NcoI" "NheI" "NotI" "PvuI" 
COMMENT         "PvuII" "XbaI" "XhoI" "FspI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 5572 5577 1 6302 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 1334 0 3070 0 4316 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 3 84 0 5080 0 5439 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1508 0 6301 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 42 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 42 0 669 0 855 0 2331 0 
COMMENT            4951 0 0 "") #5=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 4019 0 4574 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "PvuII" "cagctg" 4 0 1 1309 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 1159 0 6205 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 5 0 1502 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3871 0 0 "")) 
COMMENT       (CObList 
COMMENT        #12=(CFSignal (CObList) "hIKKalpha-KL" 4 0 0 43 1500 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "43..1497") 
COMMENT        #13=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "13..36") 
COMMENT        #14=(CFSignal (CObList) "ampicillin" 4 0 1 3575 4435 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3575..4435)") 
COMMENT        #15=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1588 1679 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1588..1679") 
COMMENT        #16=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1680 2036 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1680..2036") 
COMMENT        #17=(CFSignal (CObList) "lac promoter" 30 0 1 2114 2196 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2114..2196)") 
COMMENT        #18=(CFSignal (CObList) "SV40 ori" 33 0 0 2338 2413 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2338..2413") 
COMMENT        #19=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2444 2518 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2444..2518") 
COMMENT        #20=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2444 2518 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2444..2518)") 
COMMENT        #21=(CFSignal (CObList) "pMB1 ori" 33 0 1 2633 3034 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2633..3034)") 
COMMENT        #22=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4575 4952 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4575..4952") 
COMMENT        #23=(CFSignal (CObList) "AG promoter" 29 0 0 4958 6299 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4958..6299") 
COMMENT        #24=(CFSignal (CObList) "intron AG promoter" 15 0 0 5327 6251 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5327..6251")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #25=(CFSignal (CObList) "LMBP 5486" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #26=(CFSignal (CObList) "Last modified: 28-JAN-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
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COMMENT                    "pCAGGS-Flag-IKKalpha-KL" 2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pCAGGS-Flag-IKKalpha-KL'" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5486." 
COMMENT                          1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 05/10/07 09:32" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/28/08 11:44" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6302 bp" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 10-MAY-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #41=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
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COMMENT                     #43=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 5486") 
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COMMENT                                 "" 1))) "Last modified: 28-JAN-2008"))) 
COMMENT                   "Annotations") 
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COMMENT                           (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 43    End: 1500" 1) 
COMMENT                                   #51=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #52=(CLinePar 
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COMMENT                                        "   43..1497" 1))) "hIKKalpha-KL") 
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COMMENT                                        "Start: 3575  End: 4435 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3575..4435)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
COMMENT                     #57=(CFolderPar 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5327..6251" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                        "Start: 1680  End: 2036" 1) 
COMMENT                                   #65=(CLinePar 
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COMMENT                                        "3' flanking region of the rabbit \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1680..2036" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "Start: 13    End: 36  " 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   13..36" 1))) "FLAG epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #73=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4575..4952" 1))) "hCMV-IE enhancer") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4958..6299" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2114  End: 2196 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2114..2196)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #87=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2338  End: 2413" 1) 
COMMENT                                   #90=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2338..2413" 1))) "SV40 ori") 
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COMMENT                                        "Start: 2633  End: 3034 (Complementary)" 
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COMMENT                                   #94=(CLinePar 
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COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2633..3034)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                        "Start: 1588  End: 1679" 1) 
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COMMENT                                        "3' UTR of the rabbit \337-globin gene, incl. polyA" 
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COMMENT                                 "\337-globin polyA") 
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COMMENT                                  "SV40 polyA early") 
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COMMENT                                    #108=(CLinePar 
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COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(2444..2518)" 1))) 
COMMENT                                  "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                            "CGATCG") 
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COMMENT                            "CGCCGGCG") 
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COMMENT                                            " N1: 42 " 1))))) "KpnI: 1 site") 5 
COMMENT                            1 "GGTACC" "CCATGG") 
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COMMENT                                            " N1: 1309 " 1))))) "PvuII: 1 site") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
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COMMENT                                            " N1: 3871 " 1))))) "FspI: 1 site") 
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COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                                            " N1: 4019 " 1) 
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COMMENT                                            " N2: 4574 " 1))))) "PvuI: 2 sites") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
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COMMENT                                            " N1: 1159 " 1) 
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COMMENT                                            (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6205 " 1))))) "XbaI: 2 sites") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
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COMMENT                                            " N1: 5 " 1) 
COMMENT                                      #137=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 2) 1 2 2 0 191) 
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COMMENT                                   "NcoI (42)" "@N (@S)" 5 2.89059 13.194 0 -10 
COMMENT                                   3.6452 1.05197 #335#) 
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COMMENT                                   "NcoI (669)" "@N (@S)" 5 9.74508 10.272 0 -10 
COMMENT                                   4.08556 1.05197 #337#) 
COMMENT                             #339=(CScratch 
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COMMENT                                   "NcoI (2331)" "@N (@S)" 5 10.9381 -8.23308 0 
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COMMENT                                   "NcoI (4951)" "@N (@S)" 5 -14.0295 3.11234 0 
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FEATURES             Location/Qualifiers
     CDS             43..1500
                     /vntifkey="4"
                     /label=hIKKalpha-KL
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
     CDS             complement(3575..4435)
                     /vntifkey="4"
                     /label=ampicillin
     terminator      1588..1679
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    1680..2036
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(2114..2196)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      2338..2413
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      2444..2518
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2444..2518)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(2633..3034)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        4575..4952
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        4958..6299
                     /vntifkey="29"
                     /label=AG\promoter
     intron          5327..6251
                     /vntifkey="15"
                     /label=intron\AG\promoter
BASE COUNT     1461 a      1540 c      1690 g      1611 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg 
       61 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc 
      121 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt 
      181 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag 
      241 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg 
      301 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg 
      361 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt 
      421 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa 
      481 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg 
      541 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag 
      601 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc 
      661 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag 
      721 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa 
      781 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt 
      841 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag 
      901 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt aatggatcac 
      961 attttgaatt tgaagatagt acacatccta aatatgactt ctgcaaagat aatttctttt 
     1021 ctgttaccac ctgatgaaag tcttcattca ctacagtctc gtattgagcg tgaaactgga 
     1081 ataaatactg gttctcaaga acttctttca gagacaggaa tttctctgga tcctcggaaa 
     1141 ccagcctctc aatgtgttct agatggagtt agaggctgtg atagctatat ggtttatttg 
     1201 tttgataaaa gtaaaactgt atatgaaggg ccatttgctt ccagaagttt atctgattgt 
     1261 gtaaattata ttgtacagga cagcaaaata cagcttccaa ttatacagct gcgtaaagtg 
     1321 tgggctgaag cagtgcacta tgtgtctgga ctaaaagaag actatagcag gctctttcag 
     1381 ggacaaaggg cagcaatgtt aagtcttctt agatataatg ctaacttaac aaaaatgaag 
     1441 aacactttga tctcagcatc acaacaactg aaagctaaat tggagttttt tcacaaatga 
     1501 ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc 
     1561 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac 
     1621 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca 
     1681 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat 
     1741 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct 
     1801 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc 
     1861 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt 
     1921 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt 
     1981 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc 
     2041 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 
     2101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc 
     2161 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     2221 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa 
     2281 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     2341 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 
     2401 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc 
     2461 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     2521 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg 
     2581 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     2641 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     2701 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt 
     2761 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     2821 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     2881 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     2941 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     3001 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     3061 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     3121 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     3181 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     3241 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     3301 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     3361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     3421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     3481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     3541 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     3601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     3661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     3721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     3781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     3841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     3901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     3961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     4021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     4081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     4141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     4201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     4261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     4321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     4381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     4441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     4501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     4561 cacctgggtc gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc 
     4621 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 
     4681 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 
     4741 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 
     4801 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 
     4861 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 
     4921 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt 
     4981 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt 
     5041 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg 
     5101 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc 
     5161 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag 
     5221 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc 
     5281 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga 
     5341 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct 
     5401 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc 
     5461 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg 
     5521 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg 
     5581 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg 
     5641 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca 
     5701 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct 
     5761 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt 
     5821 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc 
     5881 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat 
     5941 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga 
     6001 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg 
     6061 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc 
     6121 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg 
     6181 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct 
     6241 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag 
     6301 aa 
//
