ID pCAGGS-Flag-IKKalpha-KL standard; circular DNA; ; 6302 BP. DE LMBP 5486. CC Accession number: LMBP 5486 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 28-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 43..1500 FT /vntifkey="4" FT /label=hIKKalpha-KL FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT CDS complement(3575..4435) FT /vntifkey="4" FT /label=ampicillin FT terminator 1588..1679 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 1680..2036 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(2114..2196) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 2338..2413 FT /vntifkey="33" FT /label=SV40\ori FT terminator 2444..2518 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2444..2518) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(2633..3034) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 4575..4952 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter 4958..6299 FT /vntifkey="29" FT /label=AG\promoter FT intron 5327..6251 FT /vntifkey="15" FT /label=intron\AG\promoter SQ Sequence 6302 BP; 1461 A; 1540 C; 1690 G; 1611 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg 60 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc 120 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt 180 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag 240 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg 300 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg 360 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt 420 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa 480 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg 540 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag 600 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc 660 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag 720 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa 780 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt 840 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag 900 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt aatggatcac 960 attttgaatt tgaagatagt acacatccta aatatgactt ctgcaaagat aatttctttt 1020 ctgttaccac ctgatgaaag tcttcattca ctacagtctc gtattgagcg tgaaactgga 1080 ataaatactg gttctcaaga acttctttca gagacaggaa tttctctgga tcctcggaaa 1140 ccagcctctc aatgtgttct agatggagtt agaggctgtg atagctatat ggtttatttg 1200 tttgataaaa gtaaaactgt atatgaaggg ccatttgctt ccagaagttt atctgattgt 1260 gtaaattata ttgtacagga cagcaaaata cagcttccaa ttatacagct gcgtaaagtg 1320 tgggctgaag cagtgcacta tgtgtctgga ctaaaagaag actatagcag gctctttcag 1380 ggacaaaggg cagcaatgtt aagtcttctt agatataatg ctaacttaac aaaaatgaag 1440 aacactttga tctcagcatc acaacaactg aaagctaaat tggagttttt tcacaaatga 1500 ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc 1560 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac 1620 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca 1680 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat 1740 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct 1800 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc 1860 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt 1920 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt 1980 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc 2040 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 2100 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc 2160 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 2220 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa 2280 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 2340 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 2400 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc 2460 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 2520 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg 2580 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 2640 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 2700 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt 2760 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 2820 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 2880 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 2940 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3000 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3060 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3120 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3180 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 3240 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 3300 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 3360 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3420 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 3480 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 3540 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 3600 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 3660 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 3720 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 3780 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 3840 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 3900 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 3960 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4020 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4080 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4140 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 4200 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 4260 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 4320 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 4380 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 4440 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 4500 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 4560 cacctgggtc gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc 4620 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 4680 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 4740 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 4800 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 4860 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 4920 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt 4980 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt 5040 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg 5100 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc 5160 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag 5220 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc 5280 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga 5340 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct 5400 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc 5460 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg 5520 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg 5580 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg 5640 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca 5700 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct 5760 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt 5820 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc 5880 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat 5940 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga 6000 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg 6060 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc 6120 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg 6180 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct 6240 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag 6300 aa 6302 //