LOCUS       pCAGGS-Flag-IKKalpha-LHS        6173 bp    DNA     circular     28-JAN-2008
DEFINITION  LMBP 5487.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5487
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-JAN-2008
            
COMMENT     VNTDATE|472487699|
COMMENT     VNTNAME|pCAGGS-Flag-IKKalpha-LHS|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-Flag-IKKalpha-LHS" 0 6173 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "FspI" "ApaI" "EcoRI" "KpnI" "NcoI" "NheI" "NotI" "PvuI" 
COMMENT         "PvuII" "XbaI" "XhoI" "Alw44I") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6173 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #3=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3742 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 4951 0 5310 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1379 0 6172 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 42 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 42 0 2202 0 4822 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 3890 0 4445 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "PvuII" "cagctg" 4 0 1 400 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "XbaI" "tctaga" 2 0 3 250 0 1144 0 6076 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 5 0 1373 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 4 425 0 905 0 2941 0 
COMMENT             4187 0 0 "")) 
COMMENT       (CObList 
COMMENT        #15=(CFSignal (CObList) "hIKKalpha-LHS" 4 0 0 43 1371 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "43..1371") 
COMMENT        #16=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "13..36") 
COMMENT        #17=(CFSignal (CObList) "ampicillin" 4 0 1 3446 4306 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3446..4306)") 
COMMENT        #18=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1459 1550 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1459..1550") 
COMMENT        #19=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1551 1907 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1551..1907") 
COMMENT        #20=(CFSignal (CObList) "lac promoter" 30 0 1 1985 2067 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1985..2067)") 
COMMENT        #21=(CFSignal (CObList) "SV40 ori" 33 0 0 2209 2284 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2209..2284") 
COMMENT        #22=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2315 2389 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2315..2389") 
COMMENT        #23=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2315 2389 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2315..2389)") 
COMMENT        #24=(CFSignal (CObList) "pMB1 ori" 33 0 1 2504 2905 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2504..2905)") 
COMMENT        #25=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4446 4823 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4446..4823") 
COMMENT        #26=(CFSignal (CObList) "AG promoter" 29 0 0 4829 6170 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4829..6170") 
COMMENT        #27=(CFSignal (CObList) "intron AG promoter" 15 0 0 5198 6122 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5198..6122")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #28=(CFSignal (CObList) "LMBP 5487" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #29=(CFSignal (CObList) "Last modified: 28-JAN-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #30=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #31=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pCAGGS-Flag-IKKalpha-LHS" 2) 5 "" 0 4) 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 1 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-Flag-IKKalpha-LHS'" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5487" 
COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/28/08 02:34" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/28/08 02:45" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6173 bp" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #41=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #46=(CRefLinePar 
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COMMENT                   0 0) 
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COMMENT                     #48=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #49=(CLinePar (CParagraph 0 (15 #28# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5487") 
COMMENT                     #50=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #51=(CLinePar (CParagraph 0 (15 #29# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 28-JAN-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #52=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #53=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 43 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 43    End: 1371" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   43..1371" 1))) "hIKKalpha-LHS") 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3446 (3 #17# 0) 1 2 2 0 327) 
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COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3446  End: 4306 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3446..4306)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5198 (3 #27# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5198  End: 6122" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5198..6122" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
COMMENT                     #67=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1551 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1551  End: 1907" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' flanking region of the rabbit \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1551..1907" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 13    End: 36  " 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   13..36" 1))) "FLAG epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #78=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #79=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4446  End: 4823" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4446..4823" 1))) "hCMV-IE enhancer") 
COMMENT                            #83=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4829 (3 #26# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
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COMMENT                                        "Start: 4829  End: 6170" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4829..6170" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 1985  End: 2067 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #90=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1985..2067)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #92=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 2209  End: 2284" 1) 
COMMENT                                   #95=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
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COMMENT                                        "   2209..2284" 1))) "SV40 ori") 
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COMMENT                                        "Start: 2504  End: 2905 (Complementary)" 
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COMMENT                                   #99=(CLinePar 
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COMMENT                                   #100=(CLinePar 
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COMMENT                                         "   complement(2504..2905)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                           "Start: 1459  End: 1550" 1) 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "3' UTR of the rabbit \337-globin gene, incl. polyA" 
COMMENT                                           1) 
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COMMENT                                     #106=(CLinePar 
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COMMENT                                           "   1459..1550" 1))) 
COMMENT                                   "\337-globin polyA") 
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COMMENT                                           "Start: 2315  End: 2389" 1) 
COMMENT                                     #109=(CLinePar 
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COMMENT                                     #110=(CLinePar 
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COMMENT                                           "   2315..2389" 1))) 
COMMENT                                   "SV40 polyA early") 
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COMMENT                                     #113=(CLinePar 
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COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(2315..2389)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                     (CObjectList 
COMMENT                      #116=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #117=(CRSFolderPar 
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COMMENT                              (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" 
COMMENT                            "CGCCGGCG") 
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FEATURES             Location/Qualifiers
     CDS             43..1371
                     /vntifkey="4"
                     /label=hIKKalpha-LHS
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
     CDS             complement(3446..4306)
                     /vntifkey="4"
                     /label=ampicillin
     terminator      1459..1550
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    1551..1907
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(1985..2067)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      2209..2284
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      2315..2389
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2315..2389)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(2504..2905)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        4446..4823
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        4829..6170
                     /vntifkey="29"
                     /label=AG\promoter
     intron          5198..6122
                     /vntifkey="15"
                     /label=intron\AG\promoter
BASE COUNT     1440 a      1525 c      1654 g      1554 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggatca cattttgaat 
       61 ttgaagatag tacacatcct aaatatgact tctgcaaaga taatttcttt tctgttacca 
      121 cctgatgaaa gtcttcattc actacagtct cgtattgagc gtgaaactgg aataaatact 
      181 ggttctcaag aacttctttc agagacagga atttctctgg atcctcggaa accagcctct 
      241 caatgtgttc tagatggagt tagaggctgt gatagctata tggtttattt gtttgataaa 
      301 agtaaaactg tatatgaagg gccatttgct tccagaagtt tatctgattg tgtaaattat 
      361 attgtacagg acagcaaaat acagcttcca attatacagc tgcgtaaagt gtgggctgaa 
      421 gcagtgcact atgtgtctgg actaaaagaa gactatagca ggctctttca gggacaaagg 
      481 gcagcaatgt taagtcttct tagatataat gctaacttaa caaaaatgaa gaacactttg 
      541 atctcagcat cacaacaact gaaagctaaa ttggagtttt ttcacaaaag cattcagctt 
      601 gacttggaga gatacagcga gcagatgacg tatgggatat cttcagaaaa aatgctaaaa 
      661 gcatggaaag aaatggaaga aaaggccatc cactatgctg aggttggtgt cattggatac 
      721 ctggaggatc agattatgtc tttgcatgct gaaatcatgg agctacagaa gagcccctat 
      781 ggaagacgtc agggagactt gatggaatct ctggaacagc gtgccattga tctatataag 
      841 cagttaaaac acagaccttc agatcactcc tacagtgaca gcacagagat ggtgaaaatc 
      901 attgtgcaca ctgtgcagag tcaggaccgt gtgctcaagg agctgtttgg tcatttgagc 
      961 aagttgttgg gctgtaagca gaagattatt gatctactcc ctaaggtgga agtggccctc 
     1021 agtaatatca aagaagctga caatactgtc atgttcatgc agggaaaaag gcagaaagaa 
     1081 atatggcatc tccttaaaat tgcctgtaca cagagttctg cccggtccct tgtaggatcc 
     1141 agtctagaag gtgcagtaac ccctcagaca tcagcatggc tgcccccgac ttcagcagaa 
     1201 catgatcatt ctctgtcatg tgtggtaact cctcaagatg gggagacttc agcacaaatg 
     1261 atagaagaaa atttgaactg ccttggccat ttaagcacta ttattcatga ggcaaatgag 
     1321 gaacagggca atagtatgat gaatcttgat tggagttggt taacagaatg actcgaggaa 
     1381 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg 
     1441 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag 
     1501 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt 
     1561 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat 
     1621 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac 
     1681 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta 
     1741 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt 
     1801 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc 
     1861 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag 
     1921 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     1981 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg 
     2041 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     2101 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     2161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     2221 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 
     2281 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg 
     2341 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     2401 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa 
     2461 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 
     2521 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     2581 gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg tgagcaaaag 
     2641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     2701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     2761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     2821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     2881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     2941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     3001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     3061 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     3121 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     3181 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     3241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     3301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     3361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     3421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     3481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     3541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     3601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     3661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     3721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     3781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     3841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     3901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     3961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     4021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     4081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     4141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     4201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     4261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     4321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     4381 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt 
     4441 cgatcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 
     4501 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     4561 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     4621 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     4681 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     4741 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     4801 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc 
     4861 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 
     4921 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg 
     4981 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 
     5041 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 
     5101 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg 
     5161 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 
     5221 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 
     5281 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 
     5341 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 
     5401 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 
     5461 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 
     5521 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 
     5581 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 
     5641 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 
     5701 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg 
     5761 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga 
     5821 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc 
     5881 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 
     5941 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct 
     6001 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 
     6061 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 
     6121 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa 
//
