ID pCAGGS-Flag-IKKalpha-LHS standard; circular DNA; ; 6173 BP. DE LMBP 5487. CC Accession number: LMBP 5487 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 28-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 43..1371 FT /vntifkey="4" FT /label=hIKKalpha-LHS FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT CDS complement(3446..4306) FT /vntifkey="4" FT /label=ampicillin FT terminator 1459..1550 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 1551..1907 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(1985..2067) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 2209..2284 FT /vntifkey="33" FT /label=SV40\ori FT terminator 2315..2389 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2315..2389) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(2504..2905) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 4446..4823 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter 4829..6170 FT /vntifkey="29" FT /label=AG\promoter FT intron 5198..6122 FT /vntifkey="15" FT /label=intron\AG\promoter SQ Sequence 6173 BP; 1440 A; 1525 C; 1654 G; 1554 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggatca cattttgaat 60 ttgaagatag tacacatcct aaatatgact tctgcaaaga taatttcttt tctgttacca 120 cctgatgaaa gtcttcattc actacagtct cgtattgagc gtgaaactgg aataaatact 180 ggttctcaag aacttctttc agagacagga atttctctgg atcctcggaa accagcctct 240 caatgtgttc tagatggagt tagaggctgt gatagctata tggtttattt gtttgataaa 300 agtaaaactg tatatgaagg gccatttgct tccagaagtt tatctgattg tgtaaattat 360 attgtacagg acagcaaaat acagcttcca attatacagc tgcgtaaagt gtgggctgaa 420 gcagtgcact atgtgtctgg actaaaagaa gactatagca ggctctttca gggacaaagg 480 gcagcaatgt taagtcttct tagatataat gctaacttaa caaaaatgaa gaacactttg 540 atctcagcat cacaacaact gaaagctaaa ttggagtttt ttcacaaaag cattcagctt 600 gacttggaga gatacagcga gcagatgacg tatgggatat cttcagaaaa aatgctaaaa 660 gcatggaaag aaatggaaga aaaggccatc cactatgctg aggttggtgt cattggatac 720 ctggaggatc agattatgtc tttgcatgct gaaatcatgg agctacagaa gagcccctat 780 ggaagacgtc agggagactt gatggaatct ctggaacagc gtgccattga tctatataag 840 cagttaaaac acagaccttc agatcactcc tacagtgaca gcacagagat ggtgaaaatc 900 attgtgcaca ctgtgcagag tcaggaccgt gtgctcaagg agctgtttgg tcatttgagc 960 aagttgttgg gctgtaagca gaagattatt gatctactcc ctaaggtgga agtggccctc 1020 agtaatatca aagaagctga caatactgtc atgttcatgc agggaaaaag gcagaaagaa 1080 atatggcatc tccttaaaat tgcctgtaca cagagttctg cccggtccct tgtaggatcc 1140 agtctagaag gtgcagtaac ccctcagaca tcagcatggc tgcccccgac ttcagcagaa 1200 catgatcatt ctctgtcatg tgtggtaact cctcaagatg gggagacttc agcacaaatg 1260 atagaagaaa atttgaactg ccttggccat ttaagcacta ttattcatga ggcaaatgag 1320 gaacagggca atagtatgat gaatcttgat tggagttggt taacagaatg actcgaggaa 1380 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg 1440 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag 1500 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt 1560 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat 1620 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac 1680 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta 1740 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt 1800 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc 1860 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag 1920 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 1980 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg 2040 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 2100 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca 2160 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 2220 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 2280 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg 2340 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 2400 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa 2460 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 2520 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 2580 gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg tgagcaaaag 2640 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 2700 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 2760 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 2820 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 2880 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 2940 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 3000 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 3060 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 3120 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 3180 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 3240 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 3300 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 3360 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 3420 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 3480 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 3540 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 3600 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 3660 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 3720 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 3780 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 3840 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 3900 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 3960 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 4020 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 4080 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 4140 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 4200 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 4260 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 4320 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 4380 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt 4440 cgatcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 4500 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 4560 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 4620 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 4680 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 4740 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 4800 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc 4860 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 4920 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg 4980 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 5040 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 5100 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg 5160 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 5220 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 5280 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 5340 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 5400 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 5460 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 5520 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 5580 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 5640 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 5700 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg 5760 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga 5820 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc 5880 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 5940 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct 6000 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 6060 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 6120 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa 6173 //