LOCUS       pCAGGS-Flag-IKKalpha-HS        5627 bp    DNA     circular     28-JAN-2008
DEFINITION  LMBP 5488.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5488
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-JAN-2008
            
COMMENT     VNTDATE|459856246|
COMMENT     VNTNAME|pCAGGS-Flag-IKKalpha-HS|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-Flag-IKKalpha-HS" 0 5627 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "Alw44I" "ApaI" "EcoRI" "KpnI" "NcoI" "NheI" "NotI" "PvuI" 
COMMENT         "XbaI" "XhoI" "FspI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 468 472 1 5627 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 359 0 2395 0 3641 0 0 
COMMENT            "") 
COMMENT        #4=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 4405 0 4764 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 833 0 5626 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 42 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1656 0 4276 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 3344 0 3899 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 598 0 5530 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 5 0 827 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3196 0 0 "")) 
COMMENT       (CObList 
COMMENT        #14=(CFSignal (CObList) "hIKKalpha-HS" 4 0 0 43 825 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "43..825") 
COMMENT        #15=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "13..36") 
COMMENT        #16=(CFSignal (CObList) "ampicillin" 4 0 1 2900 3760 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2900..3760)") 
COMMENT        #17=(CFSignal (CObList) "\337-globin polyA" 43 0 0 913 1004 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "913..1004") 
COMMENT        #18=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1005 1361 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1005..1361") 
COMMENT        #19=(CFSignal (CObList) "lac promoter" 30 0 1 1439 1521 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1439..1521)") 
COMMENT        #20=(CFSignal (CObList) "SV40 ori" 33 0 0 1663 1738 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1663..1738") 
COMMENT        #21=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1769 1843 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1769..1843") 
COMMENT        #22=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1769 1843 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1769..1843)") 
COMMENT        #23=(CFSignal (CObList) "pMB1 ori" 33 0 1 1958 2359 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1958..2359)") 
COMMENT        #24=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3900 4277 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3900..4277") 
COMMENT        #25=(CFSignal (CObList) "AG promoter" 29 0 0 4283 5624 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4283..5624") 
COMMENT        #26=(CFSignal (CObList) "intron AG promoter" 15 0 0 4652 5576 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4652..5576")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #27=(CFSignal (CObList) "LMBP 5488" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #28=(CFSignal (CObList) "Last modified: 28-JAN-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    "pCAGGS-Flag-IKKalpha-HS" 2) 5 "" 0 4) 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-Flag-IKKalpha-HS'" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5488" 
COMMENT                          1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 10/10/07 09:50" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/28/08 03:49" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5627 bp" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #40=(CFolderPar 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 10-OCT-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #43=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
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COMMENT                     #45=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #46=(CLinePar (CParagraph 0 (15 #27# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5488") 
COMMENT                     #47=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #48=(CLinePar (CParagraph 0 (15 #28# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 28-JAN-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #49=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #50=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 43 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 43    End: 825 " 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   43..825" 1))) "hIKKalpha-HS") 
COMMENT                            #55=(CFolderPar 
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COMMENT                                  (CParagraph 2900 (3 #16# 0) 1 2 2 0 327) 
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COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2900  End: 3760 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2900..3760)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
COMMENT                     #59=(CFolderPar 
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COMMENT                                   #61=(CLinePar 
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COMMENT                                        "Start: 4652  End: 5576" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4652..5576" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
COMMENT                     #64=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #65=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1005  End: 1361" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' flanking region of the rabbit \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #68=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1005..1361" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #70=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 13    End: 36  " 1) 
COMMENT                                   #73=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   13..36" 1))) "FLAG epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #75=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #76=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3900  End: 4277" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3900..4277" 1))) "hCMV-IE enhancer") 
COMMENT                            #80=(CFolderPar 
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COMMENT                                   #81=(CLinePar 
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COMMENT                                        "Start: 4283  End: 5624" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4283..5624" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #84=(CFolderPar 
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COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1439  End: 1521 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #87=(CLinePar 
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COMMENT                                   #88=(CLinePar 
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COMMENT                                        "   complement(1439..1521)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Start: 1663  End: 1738" 1) 
COMMENT                                   #92=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1663..1738" 1))) "SV40 ori") 
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COMMENT                                        "Start: 1958  End: 2359 (Complementary)" 
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COMMENT                                   #96=(CLinePar 
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COMMENT                                   #97=(CLinePar 
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COMMENT                                        "   complement(1958..2359)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                         "Start: 913   End: 1004" 1) 
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COMMENT                                         "3' UTR of the rabbit \337-globin gene, incl. polyA" 
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COMMENT                                   #103=(CLinePar 
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COMMENT                                         "   913..1004" 1))) "\337-globin polyA") 
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COMMENT                                          "Start: 1769  End: 1843" 1) 
COMMENT                                    #106=(CLinePar 
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COMMENT                                    #107=(CLinePar 
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COMMENT                                          "   1769..1843" 1))) 
COMMENT                                  "SV40 polyA early") 
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COMMENT                                    #110=(CLinePar 
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COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #111=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(1769..1843)" 1))) 
COMMENT                                  "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                     (CObjectList 
COMMENT                      #113=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #114=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" 
COMMENT                            "CGCCGGCG") 
COMMENT                      #115=(CRSFolderPar 
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COMMENT                                      #117=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 42 " 1))))) "KpnI: 1 site") 5 
COMMENT                            1 "GGTACC" "CCATGG") 
COMMENT                      #118=(CRSFolderPar 
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FEATURES             Location/Qualifiers
     CDS             43..825
                     /vntifkey="4"
                     /label=hIKKalpha-HS
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
     CDS             complement(2900..3760)
                     /vntifkey="4"
                     /label=ampicillin
     terminator      913..1004
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    1005..1361
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(1439..1521)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      1663..1738
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      1769..1843
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1769..1843)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(1958..2359)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3900..4277
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        4283..5624
                     /vntifkey="29"
                     /label=AG\promoter
     intron          4652..5576
                     /vntifkey="15"
                     /label=intron\AG\promoter
BASE COUNT     1262 a      1432 c      1547 g      1386 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccagcattca gcttgacttg 
       61 gagagataca gcgagcagat gacgtatggg atatcttcag aaaaaatgct aaaagcatgg 
      121 aaagaaatgg aagaaaaggc catccactat gctgaggttg gtgtcattgg atacctggag 
      181 gatcagatta tgtctttgca tgctgaaatc atggagctac agaagagccc ctatggaaga 
      241 cgtcagggag acttgatgga atctctggaa cagcgtgcca ttgatctata taagcagtta 
      301 aaacacagac cttcagatca ctcctacagt gacagcacag agatggtgaa aatcattgtg 
      361 cacactgtgc agagtcagga ccgtgtgctc aaggagctgt ttggtcattt gagcaagttg 
      421 ttgggctgta agcagaagat tattgatcta ctccctaagg tggaagtggc cctcagtaat 
      481 atcaaagaag ctgacaatac tgtcatgttc atgcagggaa aaaggcagaa agaaatatgg 
      541 catctcctta aaattgcctg tacacagagt tctgcccggt cccttgtagg atccagtcta 
      601 gaaggtgcag taacccctca gacatcagca tggctgcccc cgacttcagc agaacatgat 
      661 cattctctgt catgtgtggt aactcctcaa gatggggaga cttcagcaca aatgatagaa 
      721 gaaaatttga actgccttgg ccatttaagc actattattc atgaggcaaa tgaggaacag 
      781 ggcaatagta tgatgaatct tgattggagt tggttaacag aatgactcga ggaattcact 
      841 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac 
      901 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 
      961 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 
     1021 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 
     1081 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt 
     1141 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 
     1201 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 
     1261 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 
     1321 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag 
     1381 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     1441 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa 
     1501 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     1561 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     1621 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     1681 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     1741 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa 
     1801 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     1861 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc 
     1921 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     1981 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     2041 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc 
     2101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     2161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     2221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     2281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     2341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     2401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     2461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     2521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     2581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     2641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     2701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     2761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     2821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     2881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     2941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     3001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     3061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     3121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     3181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     3241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     3301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     3361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     3421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     3481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     3541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     3601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     3661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     3721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     3781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     3841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgatcg 
     3901 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 
     3961 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 
     4021 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 
     4081 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 
     4141 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 
     4201 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 
     4261 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 
     4321 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 
     4381 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg 
     4441 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 
     4501 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 
     4561 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 
     4621 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
     4681 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 
     4741 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt 
     4801 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg 
     4861 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg 
     4921 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg 
     4981 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc 
     5041 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg 
     5101 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg 
     5161 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg 
     5221 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc 
     5281 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc 
     5341 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg 
     5401 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc 
     5461 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 
     5521 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 
     5581 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa 
//
