ID pCAGGS-Flag-IKKalpha-deltaNLS standard; circular DNA; ; 7082 BP. DE LMBP 5490. CC Accession number: LMBP 5490 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 28-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 43..2280 FT /vntifkey="4" FT /label=hIKKalpha-deltaNLS FT mutation 745..753 FT /vntifkey="62" FT /label=KKK235-237AAA FT /note="WT: AAG.AAG.AAG" FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT CDS complement(4355..5215) FT /vntifkey="4" FT /label=ampicillin FT terminator 2368..2459 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 2460..2816 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(2894..2976) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin 3118..3193 FT /vntifkey="33" FT /label=SV40\ori FT terminator 3224..3298 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(3224..3298) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin complement(3413..3814) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 5355..5732 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter 5738..7079 FT /vntifkey="29" FT /label=AG\promoter FT intron 6107..7031 FT /vntifkey="15" FT /label=intron\AG\promoter SQ Sequence 7082 BP; 1699 A; 1694 C; 1888 G; 1801 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg 60 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc 120 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt 180 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag 240 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg 300 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg 360 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt 420 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa 480 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg 540 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag 600 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc 660 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag 720 ccatttacct ggcatgagaa gattgcggcc gcggatccaa agtgtatatt tgcatgtgaa 780 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt 840 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag 900 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt aatggatcac 960 attttgaatt tgaagatagt acacatccta aatatgactt ctgcaaagat aatttctttt 1020 ctgttaccac ctgatgaaag tcttcattca ctacagtctc gtattgagcg tgaaactgga 1080 ataaatactg gttctcaaga acttctttca gagacaggaa tttctctgga tcctcggaaa 1140 ccagcctctc aatgtgttct agatggagtt agaggctgtg atagctatat ggtttatttg 1200 tttgataaaa gtaaaactgt atatgaaggg ccatttgctt ccagaagttt atctgattgt 1260 gtaaattata ttgtacagga cagcaaaata cagcttccaa ttatacagct gcgtaaagtg 1320 tgggctgaag cagtgcacta tgtgtctgga ctaaaagaag actatagcag gctctttcag 1380 ggacaaaggg cagcaatgtt aagtcttctt agatataatg ctaacttaac aaaaatgaag 1440 aacactttga tctcagcatc acaacaactg aaagctaaat tggagttttt tcacaaaagc 1500 attcagcttg acttggagag atacagcgag cagatgacgt atgggatatc ttcagaaaaa 1560 atgctaaaag catggaaaga aatggaagaa aaggccatcc actatgctga ggttggtgtc 1620 attggatacc tggaggatca gattatgtct ttgcatgctg aaatcatgga gctacagaag 1680 agcccctatg gaagacgtca gggagacttg atggaatctc tggaacagcg tgccattgat 1740 ctatataagc agttaaaaca cagaccttca gatcactcct acagtgacag cacagagatg 1800 gtgaaaatca ttgtgcacac tgtgcagagt caggaccgtg tgctcaagga gctgtttggt 1860 catttgagca agttgttggg ctgtaagcag aagattattg atctactccc taaggtggaa 1920 gtggccctca gtaatatcaa agaagctgac aatactgtca tgttcatgca gggaaaaagg 1980 cagaaagaaa tatggcatct ccttaaaatt gcctgtacac agagttctgc ccggtccctt 2040 gtaggatcca gtctagaagg tgcagtaacc cctcagacat cagcatggct gcccccgact 2100 tcagcagaac atgatcattc tctgtcatgt gtggtaactc ctcaagatgg ggagacttca 2160 gcacaaatga tagaagaaaa tttgaactgc cttggccatt taagcactat tattcatgag 2220 gcaaatgagg aacagggcaa tagtatgatg aatcttgatt ggagttggtt aacagaatga 2280 ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc 2340 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac 2400 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca 2460 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat 2520 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct 2580 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc 2640 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt 2700 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt 2760 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc 2820 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 2880 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc 2940 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 3000 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa 3060 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 3120 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 3180 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc 3240 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3300 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg 3360 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 3420 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 3480 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt 3540 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3600 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3660 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3720 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3780 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3840 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3900 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3960 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 4020 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 4080 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 4140 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 4200 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 4260 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 4320 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 4380 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 4440 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 4500 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 4560 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 4620 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4680 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4740 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4800 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4860 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4920 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 4980 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 5040 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 5100 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 5160 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 5220 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 5280 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 5340 cacctgggtc gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc 5400 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 5460 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 5520 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 5580 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 5640 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 5700 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt 5760 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt 5820 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg 5880 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc 5940 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag 6000 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc 6060 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga 6120 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct 6180 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc 6240 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg 6300 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg 6360 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg 6420 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca 6480 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct 6540 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt 6600 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc 6660 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat 6720 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga 6780 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg 6840 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc 6900 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg 6960 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct 7020 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag 7080 aa 7082 //