ID pCD-MK standard; circular DNA; ; 7910 BP. DE LMBP 5536. CC Accession number: LMBP LMBP 5536 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 15-OCT-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 863..879 FT /vntifkey="30" FT /label=T7\promoter FT promoter 235..818 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT mutation 2880..2882 FT /vntifkey="62" FT /label=PCR\artefact FT /note="silent mutation; wt: ACA" FT mutation 2418..2420 FT /vntifkey="62" FT /label=stability\creator FT /note="silent mutation; wt: GCA ('GCA'GAAG recombines with the same heptamer in hAPI2)" FT mutation 936..938 FT /vntifkey="62" FT /label=Kozak FT /note="TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG" FT CDS 933..3404 FT /vntifkey="4" FT /label=hMLT FT CDS complement(6914..7774) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(5971..6373) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(5796..5879) FT /vntifkey="30" FT /label=lac\promoter FT CDS 4613..5407 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT rep_origin 4446..4521 FT /vntifkey="33" FT /label=SV40\ori FT promoter 4208..4489 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 3775..4203 FT /vntifkey="33" FT /label=f1\ori FT terminator 3505..3729 FT /vntifkey="43" FT /label=BGH\polyA FT terminator 5583..5657 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(5583..5657) FT /vntifkey="43" FT /label=SV40\polyA\late FT misc_marker 3459..3476 FT /vntifkey="22" FT /label=His-tag FT misc_marker 3414..3443 FT /vntifkey="22" FT /label=Myc\epitope SQ Sequence 7910 BP; 1979 A; 1918 C; 1968 G; 2045 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900 taagcttggt accgagctcg gatcctgcca ccatggcgct gttgggggac ccgctacagg 960 ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc ccctccggcc ggcgcgaccc 1020 tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga gctcctggat caggcgcccg 1080 agggccgggg ctggaggaga ctggcggagc tggcggggag tcgcgggcgc ctccgcctca 1140 gttgcctaga cctggagcag tgttctctta aggtactgga gcctgaagga agccccagcc 1200 tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt cacagaattg agtgatttcc 1260 tgcaggctat ggaacacact gaagttcttc agcttctcag ccccccagga ataaagatta 1320 ctgtaaaccc agagtcaaag gcagtcttgg ctggacagtt tgtgaaactg tgttgccggg 1380 caactggaca tccttttgtt caatatcagt ggttcaaaat gaataaagag attccaaatg 1440 gaaatacatc agagcttatt tttaatgcag tgcatgtaaa agatgcaggc ttttatgtct 1500 gtcgagttaa taacaatttc acctttgaat tcagccagtg gtcacagctg gatgtttgcg 1560 acatcccaga gagcttccag agaagtgttg atggcgtctc tgaatccaag ttgcaaatct 1620 gtgttgaacc aacttcccaa aagctgatgc caggcagcac attggtttta cagtgtgttg 1680 ctgttggaag ccctattcct cactaccagt ggttcaaaaa tgaattacca ttaacacatg 1740 agaccaaaaa gctatacatg gtgccttatg tggatttgga acaccaagga acctactggt 1800 gtcatgtata taatgatcga gacagtcaag atagcaagaa ggtagaaatc atcataggaa 1860 gaacagatga ggcagtggag tgcactgaag atgaattaaa taatcttggt catcctgata 1920 ataaagagca aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa 1980 atatgaatta ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga 2040 ctaacttact gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg 2100 agatgcgtaa tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat 2160 tatattatgc aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg 2220 ctccaaatcc atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc 2280 aagaaaaaga aactggactt aatgtgttct tattggatat gtgtaggaaa agaaatgact 2340 acgatgatac cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat 2400 atgccacgtg tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa 2460 tctttatgaa atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg 2520 atgaagttgc agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga 2580 ttcgaagtag tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt 2640 ctgctgaatc tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta 2700 tgtgtcttaa gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca 2760 atgtcatgat catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg 2820 cctacgttac tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca 2880 cgcctgaaga aactggcagc tacttggtat caaaggatct tcccaagcat tgcctctata 2940 ccagactcag ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat 3000 atcagtactc aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac 3060 ctctcattgc taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt 3120 gtcttatgtc taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt 3180 atcactcatt gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta 3240 atgttacacc agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt 3300 tcgctcacca tgcttcatgt cattttagta gaagtaatgt gccagtagag acaactgatg 3360 aaataccatt tagtttctct gacaggctca gaatttctga aaaagggccc ttcgaacaaa 3420 aactcatctc agaagaggat ctgaatatgc ataccggtca tcatcaccat caccattgag 3480 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 3540 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 3600 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 3660 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 3720 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 3780 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 3840 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 3900 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 3960 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 4020 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 4080 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 4140 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4200 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 4260 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 4320 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 4380 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4440 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 4500 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 4560 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 4620 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4680 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4740 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 4800 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4860 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4920 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4980 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 5040 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 5100 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 5160 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5220 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5280 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5340 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5400 cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat 5460 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 5520 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 5580 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5640 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5700 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 5760 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 5820 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 5880 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 5940 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6000 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6060 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6120 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6180 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6240 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6300 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6360 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6420 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6480 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6540 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 6600 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6660 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6720 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6780 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 6840 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 6900 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 6960 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7020 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7080 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7140 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7200 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7260 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7320 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7380 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7440 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 7500 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 7560 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 7620 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 7680 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 7740 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 7800 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 7860 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 7910 //