ID pCD-A7M8K standard; circular DNA; ; 8276 BP. DE LMBP 5537. CC Accession number: LMBP 5537 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 24-SEP-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 863..879 FT /vntifkey="30" FT /label=T7\promoter FT promoter 235..818 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT CDS 2257..3770 FT /vntifkey="4" FT /label=hMLT\fragment FT /note="exon 8 --> end" FT CDS 933..2256 FT /vntifkey="4" FT /label=hAPI2\fragment FT /note="exon 1 --> 7" FT mutation 3246..3248 FT /vntifkey="62" FT /label=PCR\artefact FT /note="silent mutation; wt: ACA" FT mutation 2184..2186 FT /vntifkey="62" FT /label=PCR\artefact FT /note="silent mutation; wt: TTA" FT mutation 2784..2786 FT /vntifkey="62" FT /label=stability\creator FT /note="silent mutation; wt: GCA ('GCA'GAAG recombines with the same heptamer in hAPI2)" FT mutation 936..938 FT /vntifkey="62" FT /label=Kozak FT /note="AAC was replaced by GAC (N2D) creating Kozak consensus sequence GCCACCATGG" FT misc_marker 3780..3809 FT /vntifkey="22" FT /label=Myc\epitope FT misc_marker 3825..3842 FT /vntifkey="22" FT /label=His-tag FT terminator complement(5949..6023) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 5949..6023 FT /vntifkey="43" FT /label=SV40\polyA\early FT CDS complement(7280..8140) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(6337..6739) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(6162..6245) FT /vntifkey="30" FT /label=lac\promoter FT CDS 4979..5773 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT rep_origin 4812..4887 FT /vntifkey="33" FT /label=SV40\ori FT promoter 4574..4855 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 4141..4569 FT /vntifkey="33" FT /label=f1\ori FT terminator 3871..4095 FT /vntifkey="43" FT /label=BGH\polyA SQ Sequence 8276 BP; 2141 A; 1955 C; 1994 G; 2186 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900 taagcttggt accgagctcg gatcctgcca ccatggacat agtagaaaac agcatattct 960 tatcaaattt gatgaaaagc gccaacacgt ttgaactgaa atacgacttg tcatgtgaac 1020 tgtaccgaat gtctacgtat tccacttttc ctgctggggt tcctgtctca gaaaggagtc 1080 ttgctcgtgc tggtttctat tacactggtg tgaatgacaa ggtcaaatgc ttctgttgtg 1140 gcctgatgct ggataactgg aaaagaggag acagtcctac tgaaaagcat aaaaagttgt 1200 atcctagctg cagattcgtt cagagtctaa attccgttaa caacttggaa gctacctctc 1260 agcctacttt tccttcttca gtaacaaatt ccacacactc attacttccg ggtacagaaa 1320 acagtggata tttccgtggc tcttattcaa actctccatc aaatcctgta aactccagag 1380 caaatcaaga tttttctgcc ttgatgagaa gttcctacca ctgtgcaatg aataacgaaa 1440 atgccagatt acttactttt cagacatggc cattgacttt tctgtcgcca acagatctgg 1500 caaaagcagg cttttactac ataggacctg gagacagagt ggcttgcttt gcctgtggtg 1560 gaaaattgag caattgggaa ccgaaggata atgctatgtc agaacacctg agacattttc 1620 ccaaatgccc atttatagaa aatcagcttc aagacacttc aagatacaca gtttctaatc 1680 tgagcatgca gacacatgca gcccgcttta aaacattctt taactggccc tctagtgttc 1740 tagttaatcc tgagcagctt gcaagtgcgg gtttttatta tgtgggtaac agtgatgatg 1800 tcaaatgctt ttgctgtgat ggtggactca ggtgttggga atctggagat gatccatggg 1860 ttcaacatgc caagtggttt ccaaggtgtg agtacttgat aagaattaaa ggacaggagt 1920 tcatccgtca agttcaagcc agttaccctc atctacttga acagctgcta tccacatcag 1980 acagcccagg agatgaaaat gcagagtcat caattatcca ttttgaacct ggagaagacc 2040 attcagaaga tgcaatcatg atgaatactc ctgtgattaa tgctgccgtg gaaatgggct 2100 ttagtagaag cctggtaaaa cagacagttc agagaaaaat cctagcaact ggagagaatt 2160 atagactagt caatgatctt gtgttggact tactcaatgc agaagatgaa ataagggaag 2220 aggagagaga aagagcaact gaggaaaaag aatcaaatga attaaataat cttggtcatc 2280 ctgataataa agagcaaaca actgaccagc ctttggcgaa ggacaaggtt gcccttttga 2340 taggaaatat gaattaccgg gagcacccca agctcaaagc tcctttggtg gatgtgtacg 2400 aattgactaa cttactgaga cagctggact tcaaagtggt ttcactgttg gatcttactg 2460 aatatgagat gcgtaatgct gtggatgagt ttttactcct tttagacaag ggagtatatg 2520 ggttattata ttatgcagga catggttatg aaaattttgg gaacagcttc atggtccccg 2580 ttgatgctcc aaatccatat aggtctgaaa attgtctgtg tgtacaaaat atactgaaat 2640 tgatgcaaga aaaagaaact ggacttaatg tgttcttatt ggatatgtgt aggaaaagaa 2700 atgactacga tgataccatt ccaatcttgg atgcactaaa agtcaccgcc aatattgtgt 2760 ttggatatgc cacgtgtcaa ggagctgaag cttttgaaat ccagcattct ggattggcaa 2820 atggaatctt tatgaaattt ttaaaagaca gattattaga agataagaaa atcactgtgt 2880 tactggatga agttgcagaa gatatgggta agtgtcacct taccaaaggc aaacaggctc 2940 tagagattcg aagtagttta tctgagaaga gagcacttac tgatccaata cagggaacag 3000 aatattctgc tgaatctctt gtgcggaatc tacagtgggc caaggctcat gaacttccag 3060 aaagtatgtg tcttaagttt gactgtggtg ttcagattca attaggattt gcagctgagt 3120 tttccaatgt catgatcatc tatacaagta tagtttacaa accaccggag ataataatgt 3180 gtgatgccta cgttactgat tttccacttg atctagatat tgatccaaaa gatgcaaata 3240 aaggcacgcc tgaagaaact ggcagctact tggtatcaaa ggatcttccc aagcattgcc 3300 tctataccag actcagttca ctgcaaaaat taaaggaaca tctagtcttc acagtatgtt 3360 tatcatatca gtactcagga ttggaagata ctgtagagga caagcaggaa gtgaatgttg 3420 ggaaacctct cattgctaaa ttagacatgc atcgaggttt gggaaggaag acttgctttc 3480 aaacttgtct tatgtctaat ggtccttacc agagttctgc agccacctca ggaggagcag 3540 ggcattatca ctcattgcaa gacccattcc atggtgttta ccattcacat cctggtaatc 3600 caagtaatgt tacaccagca gatagctgtc attgcagccg gactccagat gcatttattt 3660 caagtttcgc tcaccatgct tcatgtcatt ttagtagaag taatgtgcca gtagagacaa 3720 ctgatgaaat accatttagt ttctctgaca ggctcagaat ttctgaaaaa gggcccttcg 3780 aacaaaaact catctcagaa gaggatctga atatgcatac cggtcatcat caccatcacc 3840 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 3900 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 3960 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 4020 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 4080 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 4140 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 4200 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 4260 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 4320 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 4380 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 4440 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 4500 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 4560 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 4620 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 4680 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 4740 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4800 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 4860 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 4920 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 4980 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 5040 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 5100 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 5160 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 5220 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 5280 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 5340 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 5400 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 5460 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 5520 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 5580 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 5640 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 5700 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 5760 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 5820 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 5880 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 5940 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 6000 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 6060 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 6120 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6180 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6240 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 6300 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 6360 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 6420 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 6480 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 6540 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 6600 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 6660 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 6720 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 6780 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 6840 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 6900 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 6960 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 7020 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7080 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7140 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 7200 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 7260 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 7320 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 7380 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 7440 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 7500 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 7560 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 7620 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 7680 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 7740 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 7800 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 7860 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 7920 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 7980 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 8040 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 8100 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 8160 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 8220 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 8276 //