LOCUS       pCD-A7M8K-p20mut        8276 bp    DNA     circular     24-SEP-2008
DEFINITION  LMBP 5538.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5538
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-SEP-2008
            
COMMENT     VNTDATE|494162971|
COMMENT     VNTNAME|pCD-A7M8K-p20mut|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-A7M8K-p20mut" 0 8276 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "Alw44I" "ApaI" "BamHI" "HindIII" "NcoI" "NdeI" "PauI" 
COMMENT         "PdmI" "PstI" "SalI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 8276 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 33 0 6775 0 8021 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 3776 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 921 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 903 0 2789 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 6 611 0 932 0 1855 0 3570 0 
COMMENT            4805 0 5540 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 485 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PauI" "gcgcgc" 2 0 2 2689 0 5505 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PdmI" "gaannnnttc" 6 0 3 2566 0 4570 0 7953 0 
COMMENT            0 "") 
COMMENT        #8=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 1213 0 3522 0 5161 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 6087 0 8275 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "863..879") 
COMMENT        #11=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 235 818 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "235..818") 
COMMENT        #12=(CFSignal (CObList) "C464A" 62 0 0 2688 2693 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2688..2693") 
COMMENT        #13=(CFSignal (CObList) "hMLT mutated fragment" 4 0 0 2257 3770 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2257..3770") 
COMMENT        #14=(CFSignal (CObList) "hAPI2 fragment" 4 0 0 933 2256 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "933..2256") 
COMMENT        #15=(CFSignal (CObList) "stability creator" 62 0 0 2784 2786 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2784..2786") 
COMMENT        #16=(CFSignal (CObList) "PCR artefact" 62 0 0 3246 3248 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3246..3248") 
COMMENT        #17=(CFSignal (CObList) "PCR artefact" 62 0 0 2184 2186 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2184..2186") 
COMMENT        #18=(CFSignal (CObList) "Kozak" 62 0 0 936 938 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "936..938") 
COMMENT        #19=(CFSignal (CObList) "Myc epitope" 22 0 0 3780 3809 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3780..3809") 
COMMENT        #20=(CFSignal (CObList) "His-tag" 22 0 0 3825 3842 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3825..3842") 
COMMENT        #21=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5949 6023 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5949..6023)") 
COMMENT        #22=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5949 6023 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5949..6023") 
COMMENT        #23=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7280 8140 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(7280..8140)") 
COMMENT        #24=(CFSignal (CObList) "pMB1 ori" 33 0 1 6337 6739 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6337..6739)") 
COMMENT        #25=(CFSignal (CObList) "lac promoter" 30 0 1 6162 6245 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6162..6245)") 
COMMENT        #26=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4979 5773 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4979..5773") 
COMMENT        #27=(CFSignal (CObList) "SV40 ori" 33 0 0 4812 4887 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4812..4887") 
COMMENT        #28=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4574 4855 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4574..4855") 
COMMENT        #29=(CFSignal (CObList) "f1 ori" 33 0 0 4141 4569 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4141..4569") 
COMMENT        #30=(CFSignal (CObList) "BGH polyA" 43 0 0 3871 4095 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3871..4095")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #31=(CFSignal (CObList) "LMBP 5538" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #32=(CFSignal (CObList) "Last modified: 24-SEP-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #33=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #34=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pCD-A7M8K-p20mut" 
COMMENT                    2) 5 "" 0 4) 
COMMENT              #35=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCD-A7M8K-p20mut'" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5538." 
COMMENT                          1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: GeneCorner Plasmid Collection. Original author: LMBP Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 09/23/08 11:29" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 09/24/08 10:54" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8276 bp" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #44=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 24-SEP-2008" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #47=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 4 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Author") 
COMMENT              #50=(CFolderPar 
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COMMENT                    (CObjectList 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #53=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #54=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #55=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #56=(CLinePar (CParagraph 0 (15 #31# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5538") 
COMMENT                     #57=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #58=(CLinePar (CParagraph 0 (15 #32# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 24-SEP-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #59=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #60=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #61=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 933 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 933   End: 2256" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   933..2256" 1))) "hAPI2 fragment") 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2257 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2257  End: 3770" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2257..3770" 1))) 
COMMENT                                 "hMLT mutated fragment") 
COMMENT                            #69=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4979 (3 #26# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4979  End: 5773" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4979..5773" 1))) 
COMMENT                                 "Tn5 neomycin resistance") 
COMMENT                            #73=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7280 (3 #23# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7280  End: 8140 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7280..8140)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (4 total)") 
COMMENT                     #77=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3780 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3780  End: 3809" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3780..3809" 1))) "Myc epitope") 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3825 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3825  End: 3842" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3825..3842" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
COMMENT                     #86=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 235 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 235   End: 818 " 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   235..818" 1))) 
COMMENT                                 "hCMV-IE promoter and enhancer") 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4574 (3 #28# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4574  End: 4855" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4574..4855" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #95=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #96=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 863 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 863   End: 879 " 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   863..879" 1))) "T7 promoter") 
COMMENT                            #100=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 6162 (3 #25# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
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COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 6162  End: 6245 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #102=(CLinePar 
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COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(6162..6245)" 1))) 
COMMENT                                  "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #104=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                     #107=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4141..4569" 1))) "f1 ori") 
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COMMENT                                    (CObjectList 
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COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4812..4887" 1))) "SV40 ori") 
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COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6337 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #114=(CLinePar 
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COMMENT                                           "Start: 6337  End: 6739 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(6337..6739)" 1))) 
COMMENT                                   "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #117=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                   (CGroupPar 
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COMMENT                                    (CObjectList 
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COMMENT                                     #120=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3871..4095" 1))) "BGH polyA") 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 5949  End: 6023 (Complementary)" 
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COMMENT                                     #124=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(5949..6023)" 1))) 
COMMENT                                   "SV40 polyA late") 
COMMENT                             #126=(CFolderPar 
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COMMENT                                    (CObjectList 
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COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   5949..6023" 1))) 
COMMENT                                   "SV40 polyA early"))) "Terminator (3 total)") 
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COMMENT                                           "Start: 936   End: 938 " 1) 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "AAC was replaced by GAC (N2D) creating Kozak consensus sequence GCCACCATGG" 
COMMENT                                           1) 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   936..938" 1))) "Kozak") 
COMMENT                             #136=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2184 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #137=(CLinePar 
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COMMENT                                           "Start: 2184  End: 2186" 1) 
COMMENT                                     #138=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "silent mutation; wt: TTA" 1) 
COMMENT                                     #139=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #140=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2184..2186" 1))) "PCR artefact") 
COMMENT                             #141=(CFolderPar 
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COMMENT                                    (CObjectList 
COMMENT                                     #142=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2688  End: 2693" 1) 
COMMENT                                     #143=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "wt: TGT.AGG   (Cys Arg --> Ala Arg)   p20 mutant" 
COMMENT                                           1) 
COMMENT                                     #144=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #145=(CLinePar 
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COMMENT                                           "   2688..2693" 1))) "C464A") 
COMMENT                             #146=(CFolderPar 
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COMMENT                                    (CObjectList 
COMMENT                                     #147=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2784  End: 2786" 1) 
COMMENT                                     #148=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "silent mutation; wt: GCA  ('GCA'GAAG recombines with the same heptamer in hAPI2)" 
COMMENT                                           1) 
COMMENT                                     #149=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #150=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2784..2786" 1))) 
COMMENT                                   "stability creator") 
COMMENT                             #151=(CFolderPar 
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COMMENT                                    (CObjectList 
COMMENT                                     #152=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3246  End: 3248" 1) 
COMMENT                                     #153=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "silent mutation; wt: ACA" 1) 
COMMENT                                     #154=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #155=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3246..3248" 1))) "PCR artefact"))) 
COMMENT                           "Mutation (5 total)"))) "Feature Map") 
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COMMENT                     (CObjectList 
COMMENT                      #157=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 3776 " 1))))) "ApaI: 1 site") 
COMMENT                            5 1 "GGGCCC" "CCCGGG") 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 921 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #163=(CRSFolderPar 
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COMMENT                               #164=(CGroupPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 485 " 1))))) "NdeI: 1 site") 2 
COMMENT                            4 "CATATG" "GTATAC") 
COMMENT                      #166=(CRSFolderPar 
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COMMENT                              (CObjectList 
COMMENT                               #167=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 903 " 1) 
COMMENT                                      #169=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2789 " 1))))) 
COMMENT                             "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #170=(CRSFolderPar 
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COMMENT                              (CObjectList 
COMMENT                               #171=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 2689 " 1) 
COMMENT                                      #173=(CLinePar 
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COMMENT                                            " N2: 5505 " 1))))) "PauI: 2 sites") 
COMMENT                            1 5 "GCGCGC" "CGCGCG") 
COMMENT                      #174=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #175=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #176=(CLinePar 
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COMMENT                                            " N1: 6087 " 1) 
COMMENT                                      #177=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 8275 " 1))))) "SalI: 2 sites") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #178=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #179=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #180=(CLinePar 
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COMMENT                                            " N1: 33 " 1) 
COMMENT                                      #181=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6775 " 1) 
COMMENT                                      #182=(CLinePar 
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COMMENT                                            " N3: 8021 " 1))))) 
COMMENT                             "Alw44I: 3 sites") 1 5 "GTGCAC" "CACGTG") 
COMMENT                      #183=(CRSFolderPar 
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COMMENT                              (CObjectList 
COMMENT                               #184=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #185=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2566 " 1) 
COMMENT                                      #186=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4570 " 1) 
COMMENT                                      #187=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 7953 " 1))))) "PdmI: 3 sites") 
COMMENT                            5 5 "GAANNNNTTC" "CTTNNNNAAG") 
COMMENT                      #188=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
COMMENT                               #189=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #190=(CLinePar 
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COMMENT                                            " N1: 1213 " 1) 
COMMENT                                      #191=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3522 " 1) 
COMMENT                                      #192=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 5161 " 1))))) "PstI: 3 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
COMMENT                      #193=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #194=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 611 " 1) 
COMMENT                                      #196=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 932 " 1) 
COMMENT                                      #197=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1855 " 1) 
COMMENT                                      #198=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 3570 " 1) 
COMMENT                                      #199=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 4805 " 1) 
COMMENT                                      #200=(CLinePar 
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COMMENT                                            " N6: 5540 " 1))))) "NcoI: 6 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC"))) 
COMMENT                    "Restriction/Methylation Map")))) 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #204=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
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COMMENT          #208=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #209=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #210=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #211=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
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COMMENT          #212=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
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COMMENT          #213=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
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COMMENT          #214=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
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COMMENT          #215=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #216=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
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COMMENT          #225=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #226=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #227=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #228=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
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COMMENT          #229=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #230=(CWidgetStyle "Annotation" 0 0 1 
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COMMENT          #231=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
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COMMENT          #232=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
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COMMENT          #233=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
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COMMENT          #237=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
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COMMENT          #241=(CWidgetStyle "~Scrap" 0 0 1 
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FEATURES             Location/Qualifiers
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     mutation        2688..2693
                     /vntifkey="62"
                     /label=C464A
                     /note="wt: TGT.AGG   (Cys Arg --> Ala Arg)   p20 mutant"
     CDS             2257..3770
                     /vntifkey="4"
                     /label=hMLT\mutated\fragment
     CDS             933..2256
                     /vntifkey="4"
                     /label=hAPI2\fragment
     mutation        2784..2786
                     /vntifkey="62"
                     /label=stability\creator
                     /note="silent mutation; wt: GCA  ('GCA'GAAG recombines with the same heptamer in hAPI2)"
     mutation        3246..3248
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation; wt: ACA"
     mutation        2184..2186
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation; wt: TTA"
     mutation        936..938
                     /vntifkey="62"
                     /label=Kozak
                     /note="AAC was replaced by GAC (N2D) creating Kozak consensus sequence GCCACCATGG"
     misc_marker     3780..3809
                     /vntifkey="22"
                     /label=Myc\epitope
     misc_marker     3825..3842
                     /vntifkey="22"
                     /label=His-tag
     terminator      complement(5949..6023)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      5949..6023
                     /vntifkey="43"
                     /label=SV40\polyA\early
     CDS             complement(7280..8140)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(6337..6739)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(6162..6245)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             4979..5773
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     rep_origin      4812..4887
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        4574..4855
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4141..4569
                     /vntifkey="33"
                     /label=f1\ori
     terminator      3871..4095
                     /vntifkey="43"
                     /label=BGH\polyA
BASE COUNT     2140 a      1958 c      1994 g      2184 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accgagctcg gatcctgcca ccatggacat agtagaaaac agcatattct 
      961 tatcaaattt gatgaaaagc gccaacacgt ttgaactgaa atacgacttg tcatgtgaac 
     1021 tgtaccgaat gtctacgtat tccacttttc ctgctggggt tcctgtctca gaaaggagtc 
     1081 ttgctcgtgc tggtttctat tacactggtg tgaatgacaa ggtcaaatgc ttctgttgtg 
     1141 gcctgatgct ggataactgg aaaagaggag acagtcctac tgaaaagcat aaaaagttgt 
     1201 atcctagctg cagattcgtt cagagtctaa attccgttaa caacttggaa gctacctctc 
     1261 agcctacttt tccttcttca gtaacaaatt ccacacactc attacttccg ggtacagaaa 
     1321 acagtggata tttccgtggc tcttattcaa actctccatc aaatcctgta aactccagag 
     1381 caaatcaaga tttttctgcc ttgatgagaa gttcctacca ctgtgcaatg aataacgaaa 
     1441 atgccagatt acttactttt cagacatggc cattgacttt tctgtcgcca acagatctgg 
     1501 caaaagcagg cttttactac ataggacctg gagacagagt ggcttgcttt gcctgtggtg 
     1561 gaaaattgag caattgggaa ccgaaggata atgctatgtc agaacacctg agacattttc 
     1621 ccaaatgccc atttatagaa aatcagcttc aagacacttc aagatacaca gtttctaatc 
     1681 tgagcatgca gacacatgca gcccgcttta aaacattctt taactggccc tctagtgttc 
     1741 tagttaatcc tgagcagctt gcaagtgcgg gtttttatta tgtgggtaac agtgatgatg 
     1801 tcaaatgctt ttgctgtgat ggtggactca ggtgttggga atctggagat gatccatggg 
     1861 ttcaacatgc caagtggttt ccaaggtgtg agtacttgat aagaattaaa ggacaggagt 
     1921 tcatccgtca agttcaagcc agttaccctc atctacttga acagctgcta tccacatcag 
     1981 acagcccagg agatgaaaat gcagagtcat caattatcca ttttgaacct ggagaagacc 
     2041 attcagaaga tgcaatcatg atgaatactc ctgtgattaa tgctgccgtg gaaatgggct 
     2101 ttagtagaag cctggtaaaa cagacagttc agagaaaaat cctagcaact ggagagaatt 
     2161 atagactagt caatgatctt gtgttggact tactcaatgc agaagatgaa ataagggaag 
     2221 aggagagaga aagagcaact gaggaaaaag aatcaaatga attaaataat cttggtcatc 
     2281 ctgataataa agagcaaaca actgaccagc ctttggcgaa ggacaaggtt gcccttttga 
     2341 taggaaatat gaattaccgg gagcacccca agctcaaagc tcctttggtg gatgtgtacg 
     2401 aattgactaa cttactgaga cagctggact tcaaagtggt ttcactgttg gatcttactg 
     2461 aatatgagat gcgtaatgct gtggatgagt ttttactcct tttagacaag ggagtatatg 
     2521 ggttattata ttatgcagga catggttatg aaaattttgg gaacagcttc atggtccccg 
     2581 ttgatgctcc aaatccatat aggtctgaaa attgtctgtg tgtacaaaat atactgaaat 
     2641 tgatgcaaga aaaagaaact ggacttaatg tgttcttatt ggatatggcg cgcaaaagaa 
     2701 atgactacga tgataccatt ccaatcttgg atgcactaaa agtcaccgcc aatattgtgt 
     2761 ttggatatgc cacgtgtcaa ggagctgaag cttttgaaat ccagcattct ggattggcaa 
     2821 atggaatctt tatgaaattt ttaaaagaca gattattaga agataagaaa atcactgtgt 
     2881 tactggatga agttgcagaa gatatgggta agtgtcacct taccaaaggc aaacaggctc 
     2941 tagagattcg aagtagttta tctgagaaga gagcacttac tgatccaata cagggaacag 
     3001 aatattctgc tgaatctctt gtgcggaatc tacagtgggc caaggctcat gaacttccag 
     3061 aaagtatgtg tcttaagttt gactgtggtg ttcagattca attaggattt gcagctgagt 
     3121 tttccaatgt catgatcatc tatacaagta tagtttacaa accaccggag ataataatgt 
     3181 gtgatgccta cgttactgat tttccacttg atctagatat tgatccaaaa gatgcaaata 
     3241 aaggcacgcc tgaagaaact ggcagctact tggtatcaaa ggatcttccc aagcattgcc 
     3301 tctataccag actcagttca ctgcaaaaat taaaggaaca tctagtcttc acagtatgtt 
     3361 tatcatatca gtactcagga ttggaagata ctgtagagga caagcaggaa gtgaatgttg 
     3421 ggaaacctct cattgctaaa ttagacatgc atcgaggttt gggaaggaag acttgctttc 
     3481 aaacttgtct tatgtctaat ggtccttacc agagttctgc agccacctca ggaggagcag 
     3541 ggcattatca ctcattgcaa gacccattcc atggtgttta ccattcacat cctggtaatc 
     3601 caagtaatgt tacaccagca gatagctgtc attgcagccg gactccagat gcatttattt 
     3661 caagtttcgc tcaccatgct tcatgtcatt ttagtagaag taatgtgcca gtagagacaa 
     3721 ctgatgaaat accatttagt ttctctgaca ggctcagaat ttctgaaaaa gggcccttcg 
     3781 aacaaaaact catctcagaa gaggatctga atatgcatac cggtcatcat caccatcacc 
     3841 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     3901 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     3961 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     4021 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     4081 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     4141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     4201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     4261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 
     4321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     4381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     4441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     4501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     4561 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     4621 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     4681 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     4741 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     4801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     4861 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     4921 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 
     4981 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     5041 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     5101 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     5161 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     5221 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     5281 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     5341 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     5401 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     5461 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
     5521 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     5581 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     5641 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     5701 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     5761 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 
     5821 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
     5881 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
     5941 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     6001 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     6061 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 
     6121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     6181 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     6241 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     6301 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     6361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     6421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     6481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     6541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     6601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     6661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     6721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     6781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     6841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     6901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     6961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     7021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     7081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     7141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     7201 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     7261 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     7321 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     7381 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     7441 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     7501 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     7561 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     7621 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     7681 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     7741 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     7801 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     7861 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     7921 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     7981 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     8041 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     8101 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     8161 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     8221 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 
//
