ID pAS2.1-MLT5 standard; circular DNA; ; 10116 BP. DE LMBP 5539. CC Accession number: LMBP 5539 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 19-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 5993..6388 FT /vntifkey="4" FT /label=hMLT\fragment FT /note="N-terminal fragment (AA1 -> 132) containing death domain" FT mutation 5996..5998 FT /vntifkey="62" FT /label=Kozak FT /note="TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG" FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT /note="2µ origin, incl. region conferring stability (STB)" FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT promoter complement(8008..8091) FT /vntifkey="30" FT /label=lac\promoter FT CDS complement(9126..9986) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(8183..8585) FT /vntifkey="33" FT /label=pMB1\ori FT terminator 6416..6517 FT /vntifkey="43" FT /label=ADH1\terminator FT CDS complement(6584..7792) FT /vntifkey="4" FT /label=IS10R FT /note="E. coli IS10R (insertion sequence 10 right) transposase coding sequence" FT RBS complement(7970..8007) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS SQ Sequence 10116 BP; 2767 A; 2143 C; 2314 G; 2892 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggtattg caatacccag ctttgactca tatggccatg gaggccgaat tcatggcgct 6000 gttgggggac ccgctacagg ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc 6060 ccctccggcc ggcgcgaccc tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga 6120 gctcctggat caggcgcccg agggccgggg ctggaggaga ctggcggagc tggcggggag 6180 tcgcgggcgc ctccgcctca gttgcctaga cctggagcag tgttctctta aggtactgga 6240 gcctgaagga agccccagcc tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt 6300 cacagaattg agtgatttcc tgcaggctat ggaacacact gaagttcttc agcttctcag 6360 ccccccagga ataaagatta ctgtaaacgt cgacctgcag ccaagctaat tccgggcgaa 6420 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa 6480 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct 6540 gtaggtcagg ttgctttctc aggtatagca ctgagagatc ccctcataat ttccccaaag 6600 cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt aagtcttccc 6660 ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta 6720 cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat 6780 gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa 6840 aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca 6900 agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg 6960 gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt gccgacgctg 7020 agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc 7080 ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc 7140 tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt 7200 tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt 7260 accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa 7320 tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag 7380 ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac 7440 cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga atatcagacc 7500 agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt 7560 atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga 7620 tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag 7680 ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta 7740 attcggggca gaattggtaa agagagtcgt gtaaaatatc gagttcgcac atcttgttgt 7800 ctgattattg atttttcgcg aaaccatttg atcatatgac aagatgtgta tccaccttaa 7860 cttaatgatt tttaccaaaa tcattagggg attcatcagg gtatagcatg aggtcgctct 7920 tattgaccac acctctaccg gcatgcaagc ttggcgtaat catggtcata gctgtttcct 7980 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 8040 aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc 8100 gctttccagt cgggaaacct gtcgtgccag ctggattaat gaatcggcca acgcgcgggg 8160 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 8220 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 8280 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 8340 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 8400 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 8460 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 8520 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 8580 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 8640 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 8700 ttatcgccac tggcagcagc cactagtaac aggattagca gagcgaggta tgtaggcggt 8760 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 8820 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 8880 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 8940 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 9000 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 9060 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 9120 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 9180 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 9240 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 9300 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 9360 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 9420 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 9480 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 9540 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 9600 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 9660 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 9720 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 9780 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 9840 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 9900 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 9960 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 10020 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 10080 ggggttccgc gcacatttcc ccgaaaagtg ccacct 10116 //