ID pAS2.1-MLT3a standard; circular DNA; ; 10338 BP. DE LMBP 5540. CC Accession number: LMBP 5540 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 19-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT /note="2µ origin, incl. region conferring stability (STB)" FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT promoter complement(8230..8313) FT /vntifkey="30" FT /label=lac\promoter FT CDS complement(9348..10208) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(8405..8807) FT /vntifkey="33" FT /label=pMB1\ori FT terminator 6638..6739 FT /vntifkey="43" FT /label=ADH1\terminator FT CDS 5993..6610 FT /vntifkey="4" FT /label=hMLT\fragment FT /note="internal fragment (AA304 -> 509) containing caspase-like domain (hMLTp20)" FT mutation 6569..6571 FT /vntifkey="62" FT /label=stability\creator FT /note="silent mutation; wt: GCA ('GCA'GAAG recombines with the same heptamer in hAPI2)" FT CDS complement(6806..8014) FT /vntifkey="4" FT /label=IS10R FT /note="E. coli IS10R (insertion sequence 10 right) transposase coding sequence" FT RBS complement(8192..8229) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS SQ Sequence 10338 BP; 2891 A; 2119 C; 2330 G; 2998 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggtattg caatacccag ctttgactca tatggccatg gaggccgaat tcgtagaaat 6000 catcatagga agaacagatg aggcagtgga gtgcactgaa gatgaattaa ataatcttgg 6060 tcatcctgat aataaagagc aaacaactga ccagcctttg gcgaaggaca aggttgccct 6120 tttgatagga aatatgaatt accgggagca ccccaagctc aaagctcctt tggtggatgt 6180 gtacgaattg actaacttac tgagacagct ggacttcaaa gtggtttcac tgttggatct 6240 tactgaatat gagatgcgta atgctgtgga tgagttttta ctccttttag acaagggagt 6300 atatgggtta ttatattatg caggacatgg ttatgaaaat tttgggaaca gcttcatggt 6360 ccccgttgat gctccaaatc catataggtc tgaaaattgt ctgtgtgtac aaaatatact 6420 gaaattgatg caagaaaaag aaactggact taatgtgttc ttattggata tgtgtaggaa 6480 aagaaatgac tacgatgata ccattccaat cttggatgca ctaaaagtca ccgccaatat 6540 tgtgtttgga tatgccacgt gtcaaggagc tgaagctttt gaaatccagc attctggatt 6600 ggcaaatgga gtcgacctgc agccaagcta attccgggcg aatttcttat gatttatgat 6660 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta 6720 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc 6780 tcaggtatag cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata 6840 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag 6900 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg 6960 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac 7020 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg 7080 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt 7140 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag 7200 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg 7260 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat 7320 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct 7380 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta 7440 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa 7500 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac 7560 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct 7620 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag 7680 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg 7740 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt 7800 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg 7860 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg 7920 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg cagaattggt 7980 aaagagagtc gtgtaaaata tcgagttcgc acatcttgtt gtctgattat tgatttttcg 8040 cgaaaccatt tgatcatatg acaagatgtg tatccacctt aacttaatga tttttaccaa 8100 aatcattagg ggattcatca gggtatagca tgaggtcgct cttattgacc acacctctac 8160 cggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 8220 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 8280 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 8340 ctgtcgtgcc agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 8400 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 8460 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 8520 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 8580 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 8640 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 8700 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 8760 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 8820 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 8880 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 8940 gccactagta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 9000 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 9060 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 9120 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 9180 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 9240 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 9300 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 9360 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 9420 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 9480 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 9540 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 9600 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 9660 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 9720 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 9780 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 9840 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 9900 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 9960 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 10020 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 10080 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 10140 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 10200 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 10260 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 10320 ccccgaaaag tgccacct 10338 //