ID pAS2.1-MLT3b standard; circular DNA; ; 11283 BP. DE LMBP 5542. CC Accession number: LMBP 5542 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 19-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT /note="2µ origin, incl. region conferring stability (STB)" FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT promoter complement(9175..9258) FT /vntifkey="30" FT /label=lac\promoter FT CDS complement(10293..11153) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(9350..9752) FT /vntifkey="33" FT /label=pMB1\ori FT terminator 7583..7684 FT /vntifkey="43" FT /label=ADH1\terminator FT CDS 5993..7555 FT /vntifkey="4" FT /label=hMLT\fragment FT /note="C-terminal fragment (AA304 -> 824) containing caspase-like domain (hMLTp20)" FT mutation 6569..6571 FT /vntifkey="62" FT /label=stability\creator FT /note="silent mutation; wt: GCA ('GCA'GAAG recombines with the same heptamer in hAPI2)" FT mutation 7031..7033 FT /vntifkey="62" FT /label=PCR\artefact FT /note="silent mutation; ACA was replaced by ACG" FT CDS complement(7751..8959) FT /vntifkey="4" FT /label=IS10R FT /note="E. coli IS10R (insertion sequence 10 right) transposase coding sequence" FT RBS complement(9137..9174) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS SQ Sequence 11283 BP; 3187 A; 2299 C; 2526 G; 3271 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggtattg caatacccag ctttgactca tatggccatg gaggccgaat tcgtagaaat 6000 catcatagga agaacagatg aggcagtgga gtgcactgaa gatgaattaa ataatcttgg 6060 tcatcctgat aataaagagc aaacaactga ccagcctttg gcgaaggaca aggttgccct 6120 tttgatagga aatatgaatt accgggagca ccccaagctc aaagctcctt tggtggatgt 6180 gtacgaattg actaacttac tgagacagct ggacttcaaa gtggtttcac tgttggatct 6240 tactgaatat gagatgcgta atgctgtgga tgagttttta ctccttttag acaagggagt 6300 atatgggtta ttatattatg caggacatgg ttatgaaaat tttgggaaca gcttcatggt 6360 ccccgttgat gctccaaatc catataggtc tgaaaattgt ctgtgtgtac aaaatatact 6420 gaaattgatg caagaaaaag aaactggact taatgtgttc ttattggata tgtgtaggaa 6480 aagaaatgac tacgatgata ccattccaat cttggatgca ctaaaagtca ccgccaatat 6540 tgtgtttgga tatgccacgt gtcaaggagc tgaagctttt gaaatccagc attctggatt 6600 ggcaaatgga atctttatga aatttttaaa agacagatta ttagaagata agaaaatcac 6660 tgtgttactg gatgaagttg cagaagatat gggtaagtgt caccttacca aaggcaaaca 6720 ggctctagag attcgaagta gtttatctga gaagagagca cttactgatc caatacaggg 6780 aacagaatat tctgctgaat ctcttgtgcg gaatctacag tgggccaagg ctcatgaact 6840 tccagaaagt atgtgtctta agtttgactg tggtgttcag attcaattag gatttgcagc 6900 tgagttttcc aatgtcatga tcatctatac aagtatagtt tacaaaccac cggagataat 6960 aatgtgtgat gcctacgtta ctgattttcc acttgatcta gatattgatc caaaagatgc 7020 aaataaaggc acgcctgaag aaactggcag ctacttggta tcaaaggatc ttcccaagca 7080 ttgcctctat accagactca gttcactgca aaaattaaag gaacatctag tcttcacagt 7140 atgtttatca tatcagtact caggattgga agatactgta gaggacaagc aggaagtgaa 7200 tgttgggaaa cctctcattg ctaaattaga catgcatcga ggtttgggaa ggaagacttg 7260 ctttcaaact tgtcttatgt ctaatggtcc ttaccagagt tctgcagcca cctcaggagg 7320 agcagggcat tatcactcat tgcaagaccc attccatggt gtttaccatt cacatcctgg 7380 taatccaagt aatgttacac cagcagatag ctgtcattgc agccggactc cagatgcatt 7440 tatttcaagt ttcgctcacc atgcttcatg tcattttagt agaagtaatg tgccagtaga 7500 gacaactgat gaaataccat ttagtttctc tgacaggctc agaatttctg aaaaagtcga 7560 cctgcagcca agctaattcc gggcgaattt cttatgattt atgattttta ttattaaata 7620 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa 7680 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcactg 7740 agagatcccc tcataatttc cccaaagcgt aaccatgtgt gaataaattt tgagctagta 7800 gggttgcagc cacgagtaag tcttcccttg ttattgtgta gccagaatgc cgcaaaactt 7860 ccatgcctaa gcgaactgtt gagagtacgt ttcgatttct gactgtgtta gcctggaagt 7920 gcttgtccca accttgtttc tgagcatgaa cgcccgcaag ccaacatgtt agttgaagca 7980 tcagggcgat tagcagcatg atatcaaaac gctctgagct gctcgttcgg ctatggcgta 8040 ggcctagtcc gtaggcagga cttttcaagt ctcggaaggt ttcttcaatc tgcattcgct 8100 tcgaatagat attaacaagt tgtttgggtg ttcgaatttc aacaggtaag ttagttgcta 8160 gaacccatgg ctcctttgcc gacgctgagt agattttagg tgacgggtgg tgacaatgag 8220 tccgtgtcga gcgctgattt tttcggcctt tagagcgaga tttatacaat agaatttggc 8280 atgagattgg attgctttta gtcagcctct tatagcctaa agtctttgag tgactagatg 8340 acatatcatg taagttgctg ataggtttcc agttttccgc tcctaggtct gcatattgta 8400 cttttcctct tactcgactt aaccagtacc aacccagctt ctcaacggat ttataccatg 8460 gcactttaaa gccagcatca ctgacaatga gcggtgtggt gttactcggt agaatgctcg 8520 caaggtcggc tagaaattgg tcatgagctt tctttgaaca ttgctctgaa agcgggaacg 8580 ctttctcata aagagtaaca gaacgaccgt gtagtgcgac tgaagctcgc aataccataa 8640 gtcgtttttg ctcacgaata tcagaccagt caacaagtac aatgggcatc gtattgcccg 8700 aacagataaa gctagcatgc caacggtata cagcgagtcg ctctttgtgg aggtgacgat 8760 tacctaacaa tcggtcgatt cgtttgatgt tatgttttgt tctcgctttg gttggcaggt 8820 tacggccaag ttcggtaaga gtgagagttt tacagtcaag taatgcgtgg caagccaacg 8880 ttaagctgtt gagtcgtttt aagtgtaatt cggggcagaa ttggtaaaga gagtcgtgta 8940 aaatatcgag ttcgcacatc ttgttgtctg attattgatt tttcgcgaaa ccatttgatc 9000 atatgacaag atgtgtatcc accttaactt aatgattttt accaaaatca ttaggggatt 9060 catcagggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg 9120 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 9180 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc 9240 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 9300 gattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 9360 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 9420 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 9480 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 9540 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 9600 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 9660 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 9720 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 9780 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 9840 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tagtaacagg 9900 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 9960 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 10020 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 10080 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 10140 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 10200 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 10260 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 10320 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 10380 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 10440 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 10500 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 10560 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 10620 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 10680 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 10740 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 10800 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 10860 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 10920 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 10980 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 11040 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 11100 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 11160 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 11220 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 11280 cct 11283 //