LOCUS       pAGRIKOLA-CATMAXyZZZZZ        9535 bp    DNA     circular     21-JUN-2011
DEFINITION  LMBP 5554.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5554
            Reference: Hilson et al., Genome Research 14 (2004), 2176-2189
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 21-JUN-2011
            
            
COMMENT     VNTDATE|440685187|
COMMENT     VNTNAME|pAGRIKOLA-CATMAXyZZZZZ|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CFSignal (CObList) "Tn10R" 4 0 0 902 2110 0 (CStringList) 
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COMMENT        #3=(CFSignal (CObList) "Agri 56" 27 0 1 6950 6968 0 (CStringList) 
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COMMENT                          "Collection number: LMBP EXPRnnnnn." 1) 
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COMMENT                                        "intron of the Flaveria trinervia PDK gene encoding pyruvate orthophosphate dikinase" 
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COMMENT                                   -12.8788 -6.57266 0 -10 2.90058 1.06602 #353#) 
COMMENT                             #355=(CScratch 
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COMMENT                                   "5' gene specific primer" "@N" 1 -14.2831 
COMMENT                                   11.0946 0 -10 9.22235 1.06602 #355#) 
COMMENT                             #357=(CScratch 
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COMMENT                                   "3' gene specific primer" "@N" 1 -15.2127 
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COMMENT                                   "CaMV 35S promoter" "@N" 1 -15.6908 -8.71165 
COMMENT                                   0 -10 9.09839 1.11561 #364#) 
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COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 2.77267 2.85994) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 -6.74799 -12.8905 0 -10 5.3797 1.11561 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "SP6 promoter" 
COMMENT                                   "@N" 1 -8.10221 16.2046 0 -10 6.17302 1.11561 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   1) 
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FEATURES             Location/Qualifiers
     primer          6687..6703
                     /vntifkey="27"
                     /label=5'\gene\specific\primer
     primer          complement(6904..6920)
                     /vntifkey="27"
                     /label=3'\gene\specific\primer
     CDS             902..2110
                     /vntifkey="4"
                     /label=Tn10R
                     /note="Tn10 transposase for IS10 right"
     primer          complement(6950..6968)
                     /vntifkey="27"
                     /label=Agri\56
     promoter        5705..6655
                     /vntifkey="29"
                     /label=CaMV\35S\promoter
     rep_origin      complement(1..279)
                     /vntifkey="33"
                     /label=pMB1\ori
     primer          6524..6543
                     /vntifkey="27"
                     /label=Agri\51
     terminator      complement(3870..4129)
                     /vntifkey="43"
                     /label=nos\terminator
     misc_feature    3826..3850
                     /vntifkey="21"
                     /label=LB
                     /note="left flanking T-DNA border"
     rep_origin      3263..3810
                     /vntifkey="33"
                     /label=pSa\ori
     intron          6973..7741
                     /vntifkey="15"
                     /label=PDK\intron
                     /note="intron of the Flaveria trinervia PDK gene encoding pyruvate orthophosphate dikinase"
     promoter        complement(4716..4999)
                     /vntifkey="29"
                     /label=nos\promoter
     CDS             complement(4144..4695)
                     /vntifkey="4"
                     /label=basta\herbicide\resistance
                     /note="bar gene confers resistance to herbicide Basta"
     CDS             complement(2158..2973)
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
                     /note="nptI gene confers resistance to kanamycin and neomycin"
     primer          complement(8232..8248)
                     /vntifkey="27"
                     /label=5'\gene\specific\primer
     primer          8015..8031
                     /vntifkey="27"
                     /label=3'\gene\specific\primer
     primer          7770..7789
                     /vntifkey="27"
                     /label=Agri\64
     primer          complement(8292..8311)
                     /vntifkey="27"
                     /label=Agri\69
     misc_feature    9480..9505
                     /vntifkey="21"
                     /label=RB
                     /note="right flanking T-DNA border"
     terminator      8284..8991
                     /vntifkey="43"
                     /label=ocs\terminator
     intron          complement(7784..7965)
                     /vntifkey="15"
                     /label=CAT1\intron
                     /note="intron of the Ricinus communis (castor bean) CAT1 gene encoding catalase"
     attenuator      7989..8013
                     /vntifkey="0"
                     /label=attB2
     attenuator      complement(8251..8275)
                     /vntifkey="0"
                     /label=attB1
     attenuator      6660..6684
                     /vntifkey="0"
                     /label=attB1
     attenuator      complement(6922..6946)
                     /vntifkey="0"
                     /label=attB2
     CDS             6704..6903
                     /vntifkey="4"
                     /label=CATMAXyZZZZZ
     CDS             complement(8032..8231)
                     /vntifkey="4"
                     /label=CATMAXyZZZZZ
     promoter        5189..5205
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(9034..9050)
                     /vntifkey="30"
                     /label=SP6\promoter
     promoter        complement(9171..9254)
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     2656 a      2088 c      1991 g      2332 t       468 others
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
      781 atcaatctaa agtactgatg aatcccctaa tgatttttat caaaatcatt aagttaaggt 
      841 agatacacat cttgtcatat gatcaaatgg tttcgccaaa aatcaataat cagacaacaa 
      901 aatgtgcgaa ctcgatattt tacacgactc tctttaccaa ttctgccccg aattacactt 
      961 aaaacgactc aacagcttaa cgttggcttg ccacgcctta cttgactgta aaactctcac 
     1021 tcttaccgaa cttggccgta acctgccaac caaagcgaga acaaaacata acatcaaacg 
     1081 aatcgaccga ttgttaggta atcgtcacct ccacaaagag cgactcgctg tataccgttg 
     1141 gcatgctagc tttatctgtt cgggcaatac gatgcccatt gtacttgttg actggtctga 
     1201 tatccgtgag caaaaacggc ttatggtatt gcgagcttca gtcgcactac acggtcgttc 
     1261 tgttactctt tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga aagctcatga 
     1321 ccaatttcta gccgaccttg cgagcattct accgagtaac accacaccgc tcattgtcag 
     1381 tgatgctggc tttaaagtgc catggtataa atccgttgag aagctgggtt ggtactggtt 
     1441 aagtcgagta agaggaaaag tacaatatgc agacctagga gcggaaaact ggaaacctat 
     1501 cagcaactta catgatatgt catctagtca ctcaaagact ttaggctata agaggctgac 
     1561 taaaagcaat ccaatctcat gccaaattct attgtataaa tctcgctcta aaggccgaaa 
     1621 aaatcagcgc tcgacacgga ctcattgtca ccacccgtca cctaaaatct actcagcgtc 
     1681 ggcaaaggag ccatggattc tagcaactaa cttacctgtt gaaattcgaa cacccaaaca 
     1741 acttgttaat atctattcga agcgaatgca gattgaagaa accttccgag acttgaaaag 
     1801 tcctgcctac ggactaggct tacgccatag ccgaacgagc agctcagagc gttttgatat 
     1861 catgctgcta atcgccctga tgcttcaact aacatgttgg cttgcgggcg ttcatgctca 
     1921 gaaacaaggt tgggacaagc acttccaggc taacacagtc agaaatcgaa acgtactctc 
     1981 aacagttcgc ttaggcatgg aagttttgcg gcattctggc tacacaataa caagggaaga 
     2041 cttactcgtg gctgcaaccc tactagctca aaatttattc acacatggtt acgctttggg 
     2101 gaaattatga ggggatctct cagtctaaag tatatatgtg taacattggt ctagtgatta 
     2161 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc 
     2221 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag 
     2281 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat 
     2341 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga 
     2401 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc 
     2461 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc 
     2521 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg 
     2581 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc 
     2641 ttctaatacc tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc 
     2701 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag 
     2761 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa 
     2821 ctctggcgca tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt 
     2881 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct 
     2941 tgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta 
     3001 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag 
     3061 attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca 
     3121 gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac 
     3181 caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga 
     3241 tgatggggcg attcaggcga tccccatcca acagcccgcc gtcgagcggg cttttttatc 
     3301 cccggaagcc tgtggataga gggtagttat ccacgtgaaa ccgctaatgc cccgcaaagc 
     3361 cttgattcac ggggctttcc ggcccgctcc aaaaactatc cacgtgaaat cgctaatcag 
     3421 ggtacgtgaa atcgctaatc ggagtacgtg aaatcgctaa taaggtcacg tgaaatcgct 
     3481 aatcaaaaag gcacgtgaga acgctaatag ccctttcaga tcaacagctt gcaaacaccc 
     3541 ctcgctccgg caagtagtaa cagcaagtag tatgttcaat tagcttttca attatgaata 
     3601 tatatatcaa ttattggtcg cccttggctt gtggacaatg cgctacgcgc accggctccg 
     3661 cccgtggaca accgcaagcg gttgcccacc gtcgagcgcc tttgcccaca acccggcggc 
     3721 cggccgcaac agatcgtttt ataaattttt ttttttgaaa aagaaaaagc ccgaaaggcg 
     3781 gcaacctctc gggcttctgg atttccgatc cccggaatta gatcttggca ggatatattg 
     3841 tggtgtaacg ttatcagctt gcatgccggt cgatctagta acatagatga caccgcgcgc 
     3901 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat 
     3961 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt 
     4021 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca 
     4081 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttg actctagggg 
     4141 tcatcagatt tcggtgacgg gcaggaccgg acggggcggc accggcaggc tgaagtccag 
     4201 ctgccagaaa cccacgtcat gccagttccc gtgcttgaag ccggccgccc gcagcatgcc 
     4261 gcggggggca tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat 
     4321 gacagcgacc acgctcttga agccctgtgc ctccagggac ttcagcaggt gggtgtagag 
     4381 cgtggagccc agtcccgtcc gctggtggcg gggggagacg tacacggtcg actcggccgt 
     4441 ccagtcgtag gcgttgcgtg ccttccaggg acccgcgtag gcgatgccgg cgacctcgcc 
     4501 gtccacctcg gcgacgagcc agggatagcg ctcccgcaga cggacgaggt cgtccgtcca 
     4561 ctcctgcggt tcctgcggct cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt 
     4621 gacgatggtg cagaccgccg gcatgtccgc ctcggtggca cggcggatgt cggccgggcg 
     4681 tcgttctggg ctcatggtag atcccctcga tcgagttgag agtgaatatg agactctaat 
     4741 tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct 
     4801 agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct 
     4861 tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg cggttctgtc 
     4921 agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct 
     4981 taattctccg ctcatgatcg ataacattaa cgcttacaat ttccattcgc cattcaggct 
     5041 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 
     5101 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 
     5161 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac 
     5221 cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg 
     5281 gggatccact agttctagag cggccgctcg acgaattaat tccaatccca caaaaatctg 
     5341 agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac cctcatatca 
     5401 actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg ggttgtacaa 
     5461 aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact attacagagg 
     5521 caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg aacttcatcc 
     5581 ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca agcccaccaa 
     5641 agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat ccagccccaa 
     5701 aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt tacgatctag 
     5761 gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat gagaaggtta 
     5821 gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc tacgcagcag 
     5881 gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac cttcccaaga 
     5941 aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca gagaaagaca 
     6001 tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg cttcataaac 
     6061 caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa tctaaggcca 
     6121 tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtgaa gactggcgaa 
     6181 cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg 
     6241 gagcacgacc ctctggtcta ccccaaaaat gtcaaagata cagtctcaga agaccaaagg 
     6301 gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca 
     6361 gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat 
     6421 cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat 
     6481 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag 
     6541 caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct 
     6601 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctcgaga 
     6661 caagtttgta caaaaaagca ggcttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     6901 nnnnnnnnnn nnnnnnnnnn gacccagctt tcttgtacaa agtggtctcg aggaattcgg 
     6961 taccccagct tggtaaggaa ataattattt tcttttttcc ttttagtata aaatagttaa 
     7021 gtgatgttaa ttagtatgat tataataata tagttgttat aattgtgaaa aaataattta 
     7081 taaatatatt gtttacataa acaacatagt aatgtaaaaa aatatgacaa gtgatgtgta 
     7141 agacgaagaa gataaaagtt gagagtaagt atattatttt taatgaattt gatcgaacat 
     7201 gtaagatgat atactagcat taatatttgt tttaatcata atagtaattc tagctggttt 
     7261 gatgaattaa atatcaatga taaaatacta tagtaaaaat aagaataaat aaattaaaat 
     7321 aatatttttt tatgattaat agtttattat ataattaaat atctatacca ttactaaata 
     7381 ttttagttta aaagttaata aatattttgt tagaaattcc aatctgcttg taatttatca 
     7441 ataaacaaaa tattaaataa caagctaaag taacaaataa tatcaaacta atagaaacag 
     7501 taatctaatg taacaaaaca taatctaatg ctaatataac aaagcgcaag atctatcaat 
     7561 tttatatagt attatttttc aatcaacatt cttattaatt tctaaataat acttgtagtt 
     7621 ttattaactt ctaaatggat tgactattaa ttaaatgaat tagtcgaaca tgaataaaca 
     7681 aggtaacatg atagatcatg tcattgtgtt atcattgatc ttacatttgg attgattaca 
     7741 gttgggaagc tgggttcgaa atcgataagc ttgcgctgca gttatcatca tcatcataga 
     7801 cacacgaaat aaagtaatca gattatcagt taaagctatg taatatttac accataacca 
     7861 atcaattaaa aaatagatca gtttaaagaa agatcaaagc tcaaaaaaat aaaaagagaa 
     7921 aagggtccta accaagaaaa tgaaggagaa aaactagaaa tttacctgca caagcttgga 
     7981 tcctctagac cactttgtac aagaaagctg ggtcnnnnnn nnnnnnnnnn nnnnnnnnnn 
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga agcctgcttt tttgtacaaa cttgtctaga 
     8281 gtcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct 
     8341 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg 
     8401 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt 
     8461 caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt 
     8521 gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt 
     8581 tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg 
     8641 attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc 
     8701 gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg 
     8761 tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc 
     8821 aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt 
     8881 tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat 
     8941 cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag 
     9001 gcatgcaagc tagcttacta gtgatgcata ttctatagtg tcacctaaat ctgcggccgc 
     9061 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt 
     9121 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     9181 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 
     9241 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
     9301 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     9361 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     9421 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgaaggcct 
     9481 tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga gatct 
//
