LOCUS       pCAGGS-mMyD88s          5560 bp    DNA     circular     18-JUN-2013
DEFINITION  LMBP 5649.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5649
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 18-JUN-2013
COMMENT     VNTDATE|661441088|
COMMENT     VNTNAME|pCAGGS-mMyD88s|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-mMyD88s" 0 5560 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BstXI" "NcoI" "PvuI" "ScaI" "XbaI" "XhoI" "SacII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5560 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 2 70 0 829 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1593 0 4209 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3281 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 179 0 3391 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 764 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 5463 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 4865 0 5394 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "3' UTR of \337-globin, incl. polyA" 43 0 0 850 
COMMENT            941 0 (CStringList) (CStringList) 1 1 1 1 "850..941") 
COMMENT        #8=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            942 1298 0 (CStringList) (CStringList) 1 1 1 1 "942..1298") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 1376 1458 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1376..1458)") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 1600 1675 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1600..1675") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1706 1780 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1706..1780") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1706 1780 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1706..1780)") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 1895 2296 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1895..2296)") 
COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2837 3697 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2837..3697)") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3833 4210 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3833..4210") 
COMMENT        #16=(CFSignal (CObList) "AG promoter" 29 0 0 4216 5557 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4216..5557") 
COMMENT        #17=(CFSignal (CObList) "intron AG promoter" 15 0 0 4585 5509 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4585..5509") 
COMMENT        #18=(CFSignal (CObList) "mMyd88 fragment" 4 0 0 7 762 0 (CStringList) 
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COMMENT       (CObList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "Last modified: 18-JUN-2013" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-mMyD88s'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 05/07/13 01:38" 1) 
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COMMENT                          "Last Modified: 06/18/13 04:10" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5560 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 7-MAY-2013" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 5649") 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
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COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
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COMMENT                                        "   4216..5557" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                                        "   complement(1376..1458)" 1))) 
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COMMENT                                        "bidirectional origin of SV40" 1) 
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COMMENT                                        "   1600..1675" 1))) "SV40 ori") 
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COMMENT                                        "origin plasmid" 1) 
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COMMENT                                        "   complement(1895..2296)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                   "Feature Map") 
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COMMENT                    "Restriction/Methylation Map") 
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COMMENT        #332=(CSampFrag "pCAGGS-mMyD88s" 2072 3391 "ScaI" 5463 "XbaI" 
COMMENT              (LOGPEN 0 0 0) 1.23989e-316 4.2373e-310) 
COMMENT        #333=(CSampFrag "pCAGGS-mMyD88s" 276 5463 "XbaI" 179 "ScaI" 
COMMENT              (LOGPEN 0 0 0) 3.10144e+045 2.23811e-310))))
FEATURES             Location/Qualifiers
     terminator      850..941
                     /vntifkey="43"
                     /label=3'\UTR\of\ß-globin,\incl.\polyA
     misc_feature    942..1298
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(1376..1458)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      1600..1675
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      1706..1780
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(1706..1780)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(1895..2296)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2837..3697)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3833..4210
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        4216..5557
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          4585..5509
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     CDS             7..762
                     /vntifkey="4"
                     /label=mMyd88\fragment
BASE COUNT     1153 a      1482 c      1555 g      1370 t 
ORIGIN
        1 ttggcgatgt ctgcgggaga cccccgcgtg ggatccgggt ccctggactc cttcatgttc 
       61 tccataccct tggtcgcgct taacgtggga gtgaggcgcc gcctatcgct gttcttgaac 
      121 cctcggacgc ccgtggcggc cgactggacc ttgctcccgg aggagatggg cttcgagtac 
      181 ttggagatcc gagagctgga aacgcgccct gaccccactc gcagtttgtt ggatgcctgg 
      241 caggggcgct ctggcgcgtc tgtcggcagg ctgctagagc tgctggcctt gttagaccgt 
      301 gaggatatac tgaaggagct gaagtcgcgc atcggacaaa cgccggaact tttcgatgcc 
      361 tttatctgct actgccccaa cgatatcgag tttgtgcagg agatgatccg gcaactagaa 
      421 cagacagact atcggcttaa gttgtgtgtg tccgaccgtg acgtcctgcc gggcacctgt 
      481 gtctggtcca ttgccagcga gctaattgag aaaaggtgtc gccgcatggt ggtggttgtt 
      541 tctgacgatt atctacagag caaggaatgt gacttccaga ccaagtttgc actcagcctg 
      601 tctccaggtg tccaacagaa gcgactgatt cctattaaat acaaggcgat gaagaaggac 
      661 tttcccagta tcctgcggtt catcactata tgcgactata ccaacccttg caccaagtcc 
      721 tggttctgga cccgccttgc caaggctttg tccctgccct gactcgagga attcactcct 
      781 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa 
      841 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag 
      901 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt 
      961 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag 
     1021 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg 
     1081 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga 
     1141 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta 
     1201 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta 
     1261 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt 
     1321 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     1381 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc 
     1441 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg 
     1501 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 
     1561 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     1621 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     1681 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata 
     1741 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     1801 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc 
     1861 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     1921 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     1981 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     2041 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     2101 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     2161 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     2221 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     2281 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     2341 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     2401 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     2461 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 
     2521 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     2581 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     2641 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     2701 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     2761 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     2821 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     2881 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     2941 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     3001 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     3061 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     3121 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 
     3181 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     3241 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     3301 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     3361 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     3421 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 
     3481 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     3541 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     3601 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     3661 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     3721 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     3781 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga 
     3841 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 
     3901 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     3961 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     4021 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     4081 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     4141 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     4201 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc 
     4261 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg 
     4321 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg 
     4381 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg 
     4441 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt 
     4501 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc 
     4561 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt 
     4621 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag 
     4681 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg 
     4741 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc 
     4801 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc 
     4861 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 
     4921 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc 
     4981 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg 
     5041 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 
     5101 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg 
     5161 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 
     5221 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 
     5281 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 
     5341 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 
     5401 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 
     5461 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 
     5521 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 
//
