LOCUS       pCD-F-bio-MK-E4A        7919 bp    DNA     circular     31-JUL-2009
DEFINITION  LMBP 5680.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5680
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 31-JUL-2009
            
COMMENT     VNTDATE|465394201|
COMMENT     VNTNAME|pCD-F-bio-MK-E4A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-F-bio-MK-E4A" 0 7919 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AgeI" "Alw44I" "ApaI" "BstXI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "NheI" "NruI" "PstI" "SphI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7919 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 2973 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 33 0 6418 0 7664 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 3490 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 2523 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1608 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 912 0 2503 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 6 611 0 919 0 1012 0 3284 0 
COMMENT            4450 0 5185 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 896 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NruI" "tcgcga" 4 0 2 209 0 3429 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 1345 0 3236 0 4806 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SphI" "gcatgc" 6 0 5 1963 0 3717 0 4291 0 
COMMENT             4363 0 5158 0 0 "")) 
COMMENT       (CObList 
COMMENT        #11=(CFSignal (CObList) "FLAG epitope" 22 0 0 923 946 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 235 818 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "232..818") 
COMMENT        #13=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "863..879") 
COMMENT        #14=(CFSignal (CObList) "bio-tag" 22 0 0 953 997 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "hMLT mutant" 4 0 0 1013 3484 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "Kozak" 62 0 0 1016 1018 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1016..1016") 
COMMENT        #17=(CFSignal (CObList) "BGH polyA" 43 0 0 3516 3740 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1112..1336") 
COMMENT        #18=(CFSignal (CObList) "f1 ori" 33 0 0 3786 4214 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1382..1810") 
COMMENT        #19=(CFSignal (CObList) "SV40 ori" 33 0 0 4457 4532 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2053..2128") 
COMMENT        #20=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4219 4500 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1815..2096") 
COMMENT        #21=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4624 5418 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2220..3014") 
COMMENT        #22=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5592 5666 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3188..3262") 
COMMENT        #23=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5592 5666 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3188..3262)") 
COMMENT        #24=(CFSignal (CObList) "pMB1 ori" 33 0 1 5980 6382 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3576..3978)") 
COMMENT        #25=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6923 7783 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4519..5379)") 
COMMENT        #26=(CFSignal (CObList) "lac promoter" 30 0 1 5805 5888 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #27=(CFSignal (CObList) "E313A" 62 0 0 1949 1951 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #28=(CFSignal (CObList) "E316A" 62 0 0 1958 1960 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #29=(CFSignal (CObList) "E806A" 62 0 0 3425 3430 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #30=(CFSignal (CObList) "E653A" 62 0 0 2969 2977 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #31=(CFSignal (CObList) "stability creator" 62 0 0 2498 2500 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2500..2500") 
COMMENT        #32=(CFSignal (CObList) "PCR artefact" 62 0 0 2960 2962 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2962..2962")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #33=(CFSignal (CObList) "LMBP 5680" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #34=(CFSignal (CObList) "Last modified: 31-JUL-2009" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #35=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #36=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pCD-F-bio-MK-E4A" 
COMMENT                    2) 5 "" 0 4) 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCD-F-bio-MK-E4A'" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5680" 
COMMENT                          1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 12/10/07 12:10" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 07/31/09 10:24" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7919 bp" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #46=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #47=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #48=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #49=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #50=(CLinePar (CParagraph 0 (15 #33# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 5680") 
COMMENT                     #51=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #52=(CLinePar (CParagraph 0 (15 #34# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 31-JUL-2009"))) 
COMMENT                   "Annotations") 
COMMENT              #53=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #54=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1013 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1013  End: 3484" 1))) 
COMMENT                                 "hMLT mutant") 
COMMENT                            #57=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4624 (3 #21# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4624  End: 5418" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2220..3014" 1))) 
COMMENT                                 "Tn5 neomycin resistance") 
COMMENT                            #61=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6923 (3 #25# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6923  End: 7783 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4519..5379)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
COMMENT                     #65=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 923 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 923   End: 946 " 1))) 
COMMENT                                 "FLAG epitope") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 953 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 953   End: 997 " 1))) "bio-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
COMMENT                     #70=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 235 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 235   End: 818 " 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   232..818" 1))) 
COMMENT                                 "hCMV-IE promoter and enhancer") 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4219 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4219  End: 4500" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1815..2096" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 863 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 863   End: 879 " 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   863..879" 1))) "T7 promoter") 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5805 (3 #26# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5805  End: 5888 (Complementary)" 
COMMENT                                        1))) "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #86=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3786 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3786  End: 4214" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1382..1810" 1))) "f1 ori") 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4457 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4457  End: 4532" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2053..2128" 1))) "SV40 ori") 
COMMENT                            #95=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5980 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5980  End: 6382 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3576..3978)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #99=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #100=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 3516 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 3516  End: 3740" 1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   1112..1336" 1))) "BGH polyA") 
COMMENT                            #104=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 5592 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 5592  End: 5666" 1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   3188..3262" 1))) 
COMMENT                                  "SV40 polyA early") 
COMMENT                            #108=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 5592 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 5592  End: 5666 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #110=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #111=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(3188..3262)" 1))) 
COMMENT                                  "SV40 polyA late"))) "Terminator (3 total)") 
COMMENT                     #112=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 62 (7 62 0) 1 1 1 1 178) 
COMMENT                            (CObjectList 
COMMENT                             #113=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1016 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1016  End: 1018" 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG" 
COMMENT                                           1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1016..1016" 1))) "Kozak") 
COMMENT                             #118=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1949 (3 #27# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1949  End: 1951" 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "wt: GAG" 1))) "E313A") 
COMMENT                             #121=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1958 (3 #28# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1958  End: 1960" 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "wt: GAG" 1))) "E316A") 
COMMENT                             #124=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2498 (3 #31# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2498  End: 2500" 1) 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "silent mutation; wt: GCA  ('GCA'GAAG recombines with the same heptamer in hAPI2)" 
COMMENT                                           1) 
COMMENT                                     #127=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2500..2500" 1))) 
COMMENT                                   "stability creator") 
COMMENT                             #129=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2960 (3 #32# 0) 1 2 2 1 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2960  End: 2962" 1) 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "silent mutation; wt: ACA" 1) 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2962..2962" 1))) "PCR artefact") 
COMMENT                             #134=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2969 (3 #30# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2969  End: 2977" 1) 
COMMENT                                     #136=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "wt: GAA.ACT.GGC" 1))) "E653A") 
COMMENT                             #137=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3425 (3 #29# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #138=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3425  End: 3430" 1) 
COMMENT                                     #139=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "wt: GTA.GAG" 1))) "E806A"))) 
COMMENT                           "Mutation (7 total)"))) "Feature Map") 
COMMENT              #140=(CFolderPar 
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COMMENT                            1 5 "ACCGGT" "TGGCCA") 
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COMMENT                            5 1 "GGGCCC" "CCCGGG") 
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FEATURES             Location/Qualifiers
     misc_marker     923..946
                     /vntifkey="22"
                     /label=FLAG\epitope
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     953..997
                     /vntifkey="22"
                     /label=bio-tag
     CDS             1013..3484
                     /vntifkey="4"
                     /label=hMLT\mutant
     mutation        1016..1018
                     /vntifkey="62"
                     /label=Kozak
                     /note="TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG"
     mutation        2960..2962
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation; wt: ACA"
     mutation        2498..2500
                     /vntifkey="62"
                     /label=stability\creator
                     /note="silent mutation; wt: GCA  ('GCA'GAAG recombines with the same heptamer in hAPI2)"
     terminator      3516..3740
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      3786..4214
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      4457..4532
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        4219..4500
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             4624..5418
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      5592..5666
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5592..5666)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5980..6382)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6923..7783)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(5805..5888)
                     /vntifkey="30"
                     /label=lac\promoter
     mutation        1949..1951
                     /vntifkey="62"
                     /label=E313A
                     /note="wt: GAG"
     mutation        1958..1960
                     /vntifkey="62"
                     /label=E316A
                     /note="wt: GAG"
     mutation        3425..3430
                     /vntifkey="62"
                     /label=E806A
                     /note="wt: GTA.GAG"
     mutation        2969..2977
                     /vntifkey="62"
                     /label=E653A
                     /note="wt: GAA.ACT.GGC"
BASE COUNT     1978 a      1921 c      1978 g      2042 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caggcctgaa 
      961 cgacatcttc gaggcccaga agatcgagtg gcacgaggag gatcctgcca ccatggcgct 
     1021 gttgggggac ccgctacagg ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc 
     1081 ccctccggcc ggcgcgaccc tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga 
     1141 gctcctggat caggcgcccg agggccgggg ctggaggaga ctggcggagc tggcggggag 
     1201 tcgcgggcgc ctccgcctca gttgcctaga cctggagcag tgttctctta aggtactgga 
     1261 gcctgaagga agccccagcc tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt 
     1321 cacagaattg agtgatttcc tgcaggctat ggaacacact gaagttcttc agcttctcag 
     1381 ccccccagga ataaagatta ctgtaaaccc agagtcaaag gcagtcttgg ctggacagtt 
     1441 tgtgaaactg tgttgccggg caactggaca tccttttgtt caatatcagt ggttcaaaat 
     1501 gaataaagag attccaaatg gaaatacatc agagcttatt tttaatgcag tgcatgtaaa 
     1561 agatgcaggc ttttatgtct gtcgagttaa taacaatttc acctttgaat tcagccagtg 
     1621 gtcacagctg gatgtttgcg acatcccaga gagcttccag agaagtgttg atggcgtctc 
     1681 tgaatccaag ttgcaaatct gtgttgaacc aacttcccaa aagctgatgc caggcagcac 
     1741 attggtttta cagtgtgttg ctgttggaag ccctattcct cactaccagt ggttcaaaaa 
     1801 tgaattacca ttaacacatg agaccaaaaa gctatacatg gtgccttatg tggatttgga 
     1861 acaccaagga acctactggt gtcatgtata taatgatcga gacagtcaag atagcaagaa 
     1921 ggtagaaatc atcataggaa gaacagatgc ggcagtggca tgcactgaag atgaattaaa 
     1981 taatcttggt catcctgata ataaagagca aacaactgac cagcctttgg cgaaggacaa 
     2041 ggttgccctt ttgataggaa atatgaatta ccgggagcac cccaagctca aagctccttt 
     2101 ggtggatgtg tacgaattga ctaacttact gagacagctg gacttcaaag tggtttcact 
     2161 gttggatctt actgaatatg agatgcgtaa tgctgtggat gagtttttac tccttttaga 
     2221 caagggagta tatgggttat tatattatgc aggacatggt tatgaaaatt ttgggaacag 
     2281 cttcatggtc cccgttgatg ctccaaatcc atataggtct gaaaattgtc tgtgtgtaca 
     2341 aaatatactg aaattgatgc aagaaaaaga aactggactt aatgtgttct tattggatat 
     2401 gtgtaggaaa agaaatgact acgatgatac cattccaatc ttggatgcac taaaagtcac 
     2461 cgccaatatt gtgtttggat atgccacgtg tcaaggagct gaagcttttg aaatccagca 
     2521 ttctggattg gcaaatggaa tctttatgaa atttttaaaa gacagattat tagaagataa 
     2581 gaaaatcact gtgttactgg atgaagttgc agaagatatg ggtaagtgtc accttaccaa 
     2641 aggcaaacag gctctagaga ttcgaagtag tttatctgag aagagagcac ttactgatcc 
     2701 aatacaggga acagaatatt ctgctgaatc tcttgtgcgg aatctacagt gggccaaggc 
     2761 tcatgaactt ccagaaagta tgtgtcttaa gtttgactgt ggtgttcaga ttcaattagg 
     2821 atttgcagct gagttttcca atgtcatgat catctataca agtatagttt acaaaccacc 
     2881 ggagataata atgtgtgatg cctacgttac tgattttcca cttgatctag atattgatcc 
     2941 aaaagatgca aataaaggca cgcctgaagc aaccggtagc tacttggtat caaaggatct 
     3001 tcccaagcat tgcctctata ccagactcag ttcactgcaa aaattaaagg aacatctagt 
     3061 cttcacagta tgtttatcat atcagtactc aggattggaa gatactgtag aggacaagca 
     3121 ggaagtgaat gttgggaaac ctctcattgc taaattagac atgcatcgag gtttgggaag 
     3181 gaagacttgc tttcaaactt gtcttatgtc taatggtcct taccagagtt ctgcagccac 
     3241 ctcaggagga gcagggcatt atcactcatt gcaagaccca ttccatggtg tttaccattc 
     3301 acatcctggt aatccaagta atgttacacc agcagatagc tgtcattgca gccggactcc 
     3361 agatgcattt atttcaagtt tcgctcacca tgcttcatgt cattttagta gaagtaatgt 
     3421 gccagtcgcg acaactgatg aaataccatt tagtttctct gacaggctca gaatttctga 
     3481 aaaagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 
     3541 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 
     3601 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 
     3661 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 
     3721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 
     3781 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 
     3841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 
     3901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 
     3961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 
     4021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 
     4081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 
     4141 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 
     4201 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 
     4261 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 
     4321 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 
     4381 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 
     4441 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 
     4501 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 
     4561 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 
     4621 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 
     4681 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 
     4741 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 
     4801 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 
     4861 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 
     4921 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 
     4981 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 
     5041 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 
     5101 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 
     5161 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 
     5221 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 
     5281 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 
     5341 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 
     5401 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 
     5461 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 
     5521 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 
     5581 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     5641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     5701 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 
     5761 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 
     5821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 
     5881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 
     5941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     6001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     6061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     6121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     6181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     6241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     6301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     6361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     6421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     6481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     6541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     6601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     6661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     6721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     6781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     6841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     6901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     6961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     7021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     7081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     7141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     7201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     7261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     7321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     7381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     7441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     7501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     7561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     7621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     7681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     7741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     7801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     7861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc 
//
