ID pCD-F-HLH-MK-bioC standard; circular DNA; ; 8447 BP. DE LMBP 5681. CC Accession number: LMBP 5681 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 27-JUL-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 863..879 FT /vntifkey="30" FT /label=T7\promoter FT promoter 235..818 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT misc_marker 3965..4009 FT /vntifkey="22" FT /label=bio-tag FT CDS 1463..3934 FT /vntifkey="4" FT /label=hMLT FT CDS 968..1447 FT /vntifkey="4" FT /label=HLH\dimerization\domain FT /note="(hMLT: 968-1093; hETV6: 1094-1447)" FT misc_marker 923..946 FT /vntifkey="22" FT /label=FLAG\epitope FT mutation 971..973 FT /vntifkey="62" FT /label=Kozak FT /note="TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG" FT mutation 1466..1468 FT /vntifkey="62" FT /label=Kozak FT /note="TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG" FT mutation 3410..3412 FT /vntifkey="62" FT /label=PCR\artefact FT /note="silent mutation; wt: ACA" FT mutation 2948..2950 FT /vntifkey="62" FT /label=stability\creator FT /note="silent mutation; wt: GCA ('GCA'GAAG recombines with the same heptamer in hAPI2)" FT rep_origin complement(6508..6910) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(7451..8311) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter complement(6333..6416) FT /vntifkey="30" FT /label=lac\promoter FT terminator complement(6120..6194) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 6120..6194 FT /vntifkey="43" FT /label=SV40\polyA\early FT CDS 5152..5946 FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT rep_origin 4985..5060 FT /vntifkey="33" FT /label=SV40\ori FT promoter 4747..5028 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 4314..4742 FT /vntifkey="33" FT /label=f1\ori FT terminator 4044..4268 FT /vntifkey="43" FT /label=BGH\polyA FT CDS 3941..3964 FT /vntifkey="4" FT /label=SGSSGSSG\linker SQ Sequence 8447 BP; 2088 A; 2085 C; 2116 G; 2158 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc 960 tgccaccatg gcgctgttgg gggacccgct acaggccctg ccgccctcgg ccgcccccac 1020 ggggccgctg ctcgcccctc cggccggcgc gaccctcaac cgcctgcggg agccgctgct 1080 gcggaggctc agctacgctt cctcgacgcc acttcatgtt ccagtgcctc gagcgctcag 1140 gatggaggaa gactcgatcc gcctgcctgc gcacctgcgc ttgcagccaa tttactggag 1200 cagggatgac gtagcccagt ggctcaagtg ggctgaaaat gagttttctt taaggccaat 1260 tgacagcaac acgtttgaaa tgaatggcaa agctctcctg ctgctgacca aagaggactt 1320 tcgctatcga tctcctcatt caggtgatgt gctctatgaa ctccttcagc atattctgaa 1380 gcagaggaaa cctcggattc ttttttcacc attcttccac cctggaaact ctatacacac 1440 acagccggtt gatcctgcca ccatggcgct gttgggggac ccgctacagg ccctgccgcc 1500 ctcggccgcc cccacggggc cgctgctcgc ccctccggcc ggcgcgaccc tcaaccgcct 1560 gcgggagccg ctgctgcgga ggctcagcga gctcctggat caggcgcccg agggccgggg 1620 ctggaggaga ctggcggagc tggcggggag tcgcgggcgc ctccgcctca gttgcctaga 1680 cctggagcag tgttctctta aggtactgga gcctgaagga agccccagcc tgtgtctgct 1740 gaagttaatg ggtgaaaaag gttgcacagt cacagaattg agtgatttcc tgcaggctat 1800 ggaacacact gaagttcttc agcttctcag ccccccagga ataaagatta ctgtaaaccc 1860 agagtcaaag gcagtcttgg ctggacagtt tgtgaaactg tgttgccggg caactggaca 1920 tccttttgtt caatatcagt ggttcaaaat gaataaagag attccaaatg gaaatacatc 1980 agagcttatt tttaatgcag tgcatgtaaa agatgcaggc ttttatgtct gtcgagttaa 2040 taacaatttc acctttgaat tcagccagtg gtcacagctg gatgtttgcg acatcccaga 2100 gagcttccag agaagtgttg atggcgtctc tgaatccaag ttgcaaatct gtgttgaacc 2160 aacttcccaa aagctgatgc caggcagcac attggtttta cagtgtgttg ctgttggaag 2220 ccctattcct cactaccagt ggttcaaaaa tgaattacca ttaacacatg agaccaaaaa 2280 gctatacatg gtgccttatg tggatttgga acaccaagga acctactggt gtcatgtata 2340 taatgatcga gacagtcaag atagcaagaa ggtagaaatc atcataggaa gaacagatga 2400 ggcagtggag tgcactgaag atgaattaaa taatcttggt catcctgata ataaagagca 2460 aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa atatgaatta 2520 ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga ctaacttact 2580 gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg agatgcgtaa 2640 tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat tatattatgc 2700 aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg ctccaaatcc 2760 atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc aagaaaaaga 2820 aactggactt aatgtgttct tattggatat gtgtaggaaa agaaatgact acgatgatac 2880 cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat atgccacgtg 2940 tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa tctttatgaa 3000 atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg atgaagttgc 3060 agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga ttcgaagtag 3120 tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt ctgctgaatc 3180 tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta tgtgtcttaa 3240 gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca atgtcatgat 3300 catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg cctacgttac 3360 tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca cgcctgaaga 3420 aactggcagc tacttggtat caaaggatct tcccaagcat tgcctctata ccagactcag 3480 ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat atcagtactc 3540 aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac ctctcattgc 3600 taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt gtcttatgtc 3660 taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt atcactcatt 3720 gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta atgttacacc 3780 agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt tcgctcacca 3840 tgcttcatgt cattttagta gaagtaatgt gccagtagag acaactgatg aaataccatt 3900 tagtttctct gacaggctca gaatttctga aaaagggccc agcggaagtt caggttcatc 3960 cggaggcctg aacgacatct tcgaggccca gaagatcgag tggcacgagt aagggcccgt 4020 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 4080 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 4140 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 4200 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 4260 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 4320 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 4380 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 4440 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 4500 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 4560 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 4620 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 4680 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 4740 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 4800 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 4860 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 4920 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 4980 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 5040 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 5100 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa 5160 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 5220 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 5280 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag 5340 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 5400 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 5460 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 5520 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 5580 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 5640 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 5700 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 5760 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 5820 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 5880 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 5940 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 6000 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 6060 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 6120 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6180 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6240 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 6300 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 6360 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 6420 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 6480 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 6540 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6600 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6660 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6720 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6780 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6840 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 6900 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6960 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 7020 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 7080 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 7140 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 7200 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 7260 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 7320 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 7380 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 7440 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 7500 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 7560 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7620 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7680 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 7740 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 7800 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7860 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7920 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7980 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 8040 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 8100 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 8160 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 8220 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 8280 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 8340 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 8400 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc 8447 //