LOCUS pCD-F-HLH-MK-E4A-bio 8432 bp DNA circular 27-JUL-2009 DEFINITION LMBP 5683. SOURCE ORGANISM COMMENT Accession number: LMBP 5683 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 27-JUL-2009 COMMENT VNTDATE|501510223| COMMENT VNTNAME|pCD-F-HLH-MK-E4A-bio| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pCD-F-HLH-MK-E4A-bio" 0 8432 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 COMMENT 0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) COMMENT (CWordArray) COMMENT (CStringList "AgeI" "Alw44I" "BstXI" "EcoRI" "HindIII" "NcoI" "NruI" COMMENT "PstI" "SphI" "XhoI" "XmnI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 8432 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 3423 0 0 "") COMMENT #1=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 33 0 6931 0 8177 0 0 "") COMMENT #2=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 2 1195 0 2973 0 0 "") COMMENT #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2058 0 0 "") COMMENT #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 912 0 2953 0 0 "") COMMENT #5=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 7 611 0 919 0 967 0 1462 0 COMMENT 3734 0 4963 0 5698 0 0 "") COMMENT #6=(CRSite (CStringList) "NruI" "tcgcga" 4 0 2 209 0 3879 0 0 "") COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 1795 0 3686 0 5319 0 0 "") COMMENT #8=(CRSite (CStringList) "SphI" "gcatgc" 6 0 5 2413 0 4230 0 4804 0 4876 COMMENT 0 5671 0 0 "") COMMENT #9=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1129 0 0 "") COMMENT #10=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 5 1363 0 1816 0 2730 0 COMMENT 4728 0 8109 0 0 "")) COMMENT (CObList COMMENT #11=(CFSignal (CObList) "FLAG epitope" 22 0 0 923 946 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #12=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 235 818 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "232..818") COMMENT #13=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "863..879") COMMENT #14=(CFSignal (CObList) "bio-tag" 22 0 0 3950 3994 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #15=(CFSignal (CObList) "Kozak" 62 0 0 971 973 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "971..971") COMMENT #16=(CFSignal (CObList) "BGH polyA" 43 0 0 4029 4253 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1112..1336") COMMENT #17=(CFSignal (CObList) "f1 ori" 33 0 0 4299 4727 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1382..1810") COMMENT #18=(CFSignal (CObList) "SV40 ori" 33 0 0 4970 5045 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2053..2128") COMMENT #19=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4732 5013 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1815..2096") COMMENT #20=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 5137 5931 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2220..3014") COMMENT #21=(CFSignal (CObList) "SV40 polyA early" 43 0 0 6105 6179 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3188..3262") COMMENT #22=(CFSignal (CObList) "SV40 polyA late" 43 0 1 6105 6179 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(3188..3262)") COMMENT #23=(CFSignal (CObList) "pMB1 ori" 33 0 1 6493 6895 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3576..3978)") COMMENT #24=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7436 8296 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(4519..5379)") COMMENT #25=(CFSignal (CObList) "lac promoter" 30 0 1 6318 6401 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #26=(CFSignal (CObList) "hMLT mutant" 4 0 0 1463 3934 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #27=(CFSignal (CObList) "Kozak" 62 0 0 1466 1468 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #28=(CFSignal (CObList) "E653A" 62 0 0 3419 3427 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #29=(CFSignal (CObList) "E806A" 62 0 0 3875 3880 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #30=(CFSignal (CObList) "E313A" 62 0 0 2399 2401 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #31=(CFSignal (CObList) "E316A" 62 0 0 2408 2410 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #32=(CFSignal (CObList) "HLH dimerization domain" 4 0 0 968 1447 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #33=(CFSignal (CObList) "stability creator" 62 0 0 2948 2950 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2950..2950") COMMENT #34=(CFSignal (CObList) "PCR artefact" 62 0 0 3410 3412 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3412..3412")) (CObList) (CObList) (CObList) COMMENT (CObList) COMMENT (CObList COMMENT #35=(CFSignal (CObList) "LMBP 5683" 2 0 0 0 0 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #36=(CFSignal (CObList) "Last modified: 27-JUL-2009" 2 0 0 0 0 0 COMMENT (CStringList) (CStringList) 1 1 0 0 "")) (CObList) COMMENT (CTextView 0 COMMENT #37=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #38=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) COMMENT "pCD-F-HLH-MK-E4A-bio" 2) 5 "" 0 4) COMMENT #39=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) COMMENT (CObjectList COMMENT #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'pCD-F-HLH-MK-E4A-bio'" 1) COMMENT #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 5683" COMMENT 1) COMMENT #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Local object" 1) COMMENT #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 12/12/08 12:23" 1) COMMENT #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 07/27/09 04:32" 1) COMMENT #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 8432 bp" 1) COMMENT #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #48=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) COMMENT "Standard Fields") COMMENT #49=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #50=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #51=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) COMMENT (CObjectList COMMENT #52=(CLinePar (CParagraph 0 (15 #35# 0) 1 2 2 0 180) COMMENT "" 1))) "LMBP 5683") COMMENT #53=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) COMMENT (CObjectList COMMENT #54=(CLinePar (CParagraph 0 (15 #36# 0) 1 2 2 0 180) COMMENT "" 1))) "Last modified: 27-JUL-2009"))) COMMENT "Annotations") COMMENT #55=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #56=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #57=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 968 (3 #32# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 968 End: 1447" 1) COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "(hMLT: 968-1093; hETV6: 1094-1447)" 1))) COMMENT "HLH dimerization domain") COMMENT #60=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1463 (3 #26# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1463 End: 3934" 1))) COMMENT "hMLT mutant") COMMENT #62=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5137 (3 #20# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5137 End: 5931" 1) COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2220..3014" 1))) COMMENT "Tn5 neomycin resistance") COMMENT #66=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 7436 (3 #24# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 7436 End: 8296 (Complementary)" COMMENT 1) COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4519..5379)" 1))) COMMENT "ampicillin resistance"))) "CDS (4 total)") COMMENT #70=(CFolderPar COMMENT (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #71=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 923 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 923 End: 946 " 1))) COMMENT "FLAG epitope") COMMENT #73=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3950 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3950 End: 3994" 1))) "bio-tag"))) COMMENT "Misc. Marker (2 total)") COMMENT #75=(CFolderPar COMMENT (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #76=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 235 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 235 End: 818 " 1) COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 232..818" 1))) COMMENT "hCMV-IE promoter and enhancer") COMMENT #80=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4732 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4732 End: 5013" 1) COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1815..2096" 1))) COMMENT "SV40 early promoter"))) COMMENT "Promoter Eukaryotic (2 total)") COMMENT #84=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #85=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 863 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 863 End: 879 " 1) COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 863..879" 1))) "T7 promoter") COMMENT #89=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6318 (3 #25# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6318 End: 6401 (Complementary)" COMMENT 1))) "lac promoter"))) COMMENT "Promoter Prokaryotic (2 total)") COMMENT #91=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #92=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4299 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4299 End: 4727" 1) COMMENT #94=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1382..1810" 1))) "f1 ori") COMMENT #96=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4970 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4970 End: 5045" 1) COMMENT #98=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #99=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2053..2128" 1))) "SV40 ori") COMMENT #100=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6493 (3 #23# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6493 End: 6895 (Complementary)" COMMENT 1) COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #103=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3576..3978)" 1))) COMMENT "pMB1 ori"))) "Replication Origin (3 total)") COMMENT #104=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #105=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4029 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #106=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4029 End: 4253" 1) COMMENT #107=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #108=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1112..1336" 1))) "BGH polyA") COMMENT #109=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6105 (3 #21# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #110=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6105 End: 6179" 1) COMMENT #111=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #112=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3188..3262" 1))) COMMENT "SV40 polyA early") COMMENT #113=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6105 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #114=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6105 End: 6179 (Complementary)" COMMENT 1) COMMENT #115=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #116=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3188..3262)" 1))) COMMENT "SV40 polyA late"))) "Terminator (3 total)") COMMENT #117=(CFolderPar COMMENT (CGroupPar (CParagraph 62 (7 62 0) 1 1 1 1 178) COMMENT (CObjectList COMMENT #118=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 971 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #119=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 971 End: 973 " 1) COMMENT #120=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG" COMMENT 1) COMMENT #121=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #122=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 971..971" 1))) "Kozak") COMMENT #123=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1466 (3 #27# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #124=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1466 End: 1468" 1) COMMENT #125=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG" COMMENT 1))) "Kozak") COMMENT #126=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2399 (3 #30# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #127=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2399 End: 2401" 1) COMMENT #128=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "wt: GAG" 1))) "E313A") COMMENT #129=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2408 (3 #31# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #130=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2408 End: 2410" 1) COMMENT #131=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "wt: GAG" 1))) "E316A") COMMENT #132=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2948 (3 #33# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #133=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2948 End: 2950" 1) COMMENT #134=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "silent mutation; wt: GCA ('GCA'GAAG recombines with the same heptamer in hAPI2)" COMMENT 1) COMMENT #135=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #136=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2950..2950" 1))) COMMENT "stability creator") COMMENT #137=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3410 (3 #34# 0) 1 2 2 1 194) COMMENT (CObjectList COMMENT #138=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3410 End: 3412" 1) COMMENT #139=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "silent mutation; wt: ACA" 1) COMMENT #140=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #141=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3412..3412" 1))) "PCR artefact") COMMENT #142=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3419 (3 #28# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #143=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3419 End: 3427" 1) COMMENT #144=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "wt: GAA.ACT.GGC" 1))) "E653A") COMMENT #145=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3875 (3 #29# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #146=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3875 End: 3880" 1) COMMENT #147=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "wt: GTA.GAG" 1))) "E806A"))) COMMENT "Mutation (8 total)"))) "Feature Map") COMMENT #148=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #149=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #150=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #151=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 3423 " 1))))) "AgeI: 1 site") COMMENT 1 5 "ACCGGT" "TGGCCA") COMMENT #152=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #153=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #154=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 2058 " 1))))) "EcoRI: 1 site") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #155=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #156=(CGroupPar COMMENT (CParagraph 0 (10 #9# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #157=(CLinePar COMMENT (CParagraph 0 (1 #9# 1) 1 2 2 0 191) COMMENT " N1: 1129 " 1))))) "XhoI: 1 site") COMMENT 1 5 "CTCGAG" "GAGCTC") COMMENT #158=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #159=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #160=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 1195 " 1) COMMENT #161=(CLinePar COMMENT (CParagraph 0 (1 #2# 2) 1 2 2 0 191) COMMENT " N2: 2973 " 1))))) "BstXI: 2 sites") COMMENT 8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") COMMENT #162=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #163=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #164=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 912 " 1) COMMENT #165=(CLinePar COMMENT (CParagraph 0 (1 #4# 2) 1 2 2 0 191) COMMENT " N2: 2953 " 1))))) COMMENT "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") COMMENT #166=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #167=(CGroupPar COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #168=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT " N1: 209 " 1) COMMENT #169=(CLinePar COMMENT (CParagraph 0 (1 #6# 2) 1 2 2 0 191) COMMENT " N2: 3879 " 1))))) "NruI: 2 sites") COMMENT 3 3 "TCGCGA" "AGCGCT") COMMENT #170=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #171=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #172=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 33 " 1) COMMENT #173=(CLinePar COMMENT (CParagraph 0 (1 #1# 2) 1 2 2 0 191) COMMENT " N2: 6931 " 1) COMMENT #174=(CLinePar COMMENT (CParagraph 0 (1 #1# 3) 1 2 2 0 191) COMMENT " N3: 8177 " 1))))) COMMENT "Alw44I: 3 sites") 1 5 "GTGCAC" "CACGTG") COMMENT #175=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #176=(CGroupPar COMMENT (CParagraph 0 (10 #7# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #177=(CLinePar COMMENT (CParagraph 0 (1 #7# 1) 1 2 2 0 191) COMMENT " N1: 1795 " 1) COMMENT #178=(CLinePar COMMENT (CParagraph 0 (1 #7# 2) 1 2 2 0 191) COMMENT " N2: 3686 " 1) COMMENT #179=(CLinePar COMMENT (CParagraph 0 (1 #7# 3) 1 2 2 0 191) COMMENT " N3: 5319 " 1))))) "PstI: 3 sites") COMMENT 5 1 "CTGCAG" "GACGTC") COMMENT #180=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 5 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #181=(CGroupPar COMMENT (CParagraph 0 (10 #8# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #182=(CLinePar COMMENT (CParagraph 0 (1 #8# 1) 1 2 2 0 191) COMMENT " N1: 2413 " 1) COMMENT #183=(CLinePar COMMENT (CParagraph 0 (1 #8# 2) 1 2 2 0 191) COMMENT " N2: 4230 " 1) COMMENT #184=(CLinePar COMMENT (CParagraph 0 (1 #8# 3) 1 2 2 0 191) COMMENT " N3: 4804 " 1) COMMENT #185=(CLinePar COMMENT (CParagraph 0 (1 #8# 4) 1 2 2 0 191) COMMENT " N4: 4876 " 1) COMMENT #186=(CLinePar COMMENT (CParagraph 0 (1 #8# 5) 1 2 2 0 191) COMMENT " N5: 5671 " 1))))) "SphI: 5 sites") COMMENT 5 1 "GCATGC" "CGTACG") COMMENT #187=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 5 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #188=(CGroupPar COMMENT (CParagraph 0 (10 #10# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #189=(CLinePar COMMENT (CParagraph 0 (1 #10# 1) 1 2 2 0 191) COMMENT " N1: 1363 " 1) COMMENT #190=(CLinePar COMMENT (CParagraph 0 (1 #10# 2) 1 2 2 0 191) COMMENT " N2: 1816 " 1) COMMENT #191=(CLinePar COMMENT (CParagraph 0 (1 #10# 3) 1 2 2 0 191) COMMENT " N3: 2730 " 1) COMMENT #192=(CLinePar COMMENT (CParagraph 0 (1 #10# 4) 1 2 2 0 191) COMMENT " N4: 4728 " 1) COMMENT #193=(CLinePar COMMENT (CParagraph 0 (1 #10# 5) 1 2 2 0 191) COMMENT " N5: 8109 " 1))))) "XmnI: 5 sites") COMMENT 5 5 "GAANNNNTTC" "CTTNNNNAAG") COMMENT #194=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 7 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #195=(CGroupPar COMMENT (CParagraph 0 (10 #5# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #196=(CLinePar COMMENT (CParagraph 0 (1 #5# 1) 1 2 2 0 191) COMMENT " N1: 611 " 1) COMMENT #197=(CLinePar COMMENT (CParagraph 0 (1 #5# 2) 1 2 2 0 191) COMMENT " N2: 919 " 1) COMMENT #198=(CLinePar COMMENT (CParagraph 0 (1 #5# 3) 1 2 2 0 191) COMMENT " N3: 967 " 1) COMMENT #199=(CLinePar COMMENT (CParagraph 0 (1 #5# 4) 1 2 2 0 191) COMMENT " N4: 1462 " 1) COMMENT #200=(CLinePar COMMENT (CParagraph 0 (1 #5# 5) 1 2 2 0 191) COMMENT " N5: 3734 " 1) COMMENT #201=(CLinePar COMMENT (CParagraph 0 (1 #5# 6) 1 2 2 0 191) COMMENT " N6: 4963 " 1) COMMENT #202=(CLinePar COMMENT (CParagraph 0 (1 #5# 7) 1 2 2 0 191) COMMENT " N7: 5698 " 1))))) "NcoI: 7 sites") COMMENT 1 5 "CCATGG" "GGTACC"))) COMMENT "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #203=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 5 "@N (@S)" 0) COMMENT #204=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #205=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 COMMENT "@L bp" 0) COMMENT #206=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) COMMENT #207=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 COMMENT 0 0) COMMENT #208=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 8388736 0) 0 0 0) COMMENT #209=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) COMMENT 0 0 0) COMMENT #210=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #211=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #212=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) COMMENT #213=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 5 "@N (@S)" 0) COMMENT #214=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #215=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #216=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 COMMENT "@N (@H%)" 0) COMMENT #217=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #218=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #219=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #220=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #221=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) #222=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #223=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #224=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #225=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #226=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #227=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #228=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #229=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #230=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #231=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #232=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #233=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #234=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #235=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #236=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 COMMENT 65535 "@N" 0) COMMENT #237=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 3342336) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #238=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #239=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #240=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 COMMENT 5 "@N (@S)" 0) COMMENT #241=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 COMMENT 1 5 "@N (@S)" 0) COMMENT #242=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32768 1 5 "@N (@S)" 0) COMMENT #243=(CWidgetStyle "~Scrap" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 COMMENT "@L bp" 0) COMMENT #244=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16711935 1 5 "@N (@S)" 0) COMMENT #245=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #246=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16711680 1 5 "@N (@S)" 0) COMMENT #247=(CWidgetStyle "Intron Label" 1 (LOGPEN 0 0 3342336) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #248=(CWidgetStyle "Misc_Feature Label" 1 (LOGPEN 0 0 3342336) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #249=(CWidgetStyle "Promoter_E Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #250=(CWidgetStyle "Promoter_P Label" 1 (LOGPEN 0 0 3342336) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #251=(CWidgetStyle "Rep_Origin Label" 1 (LOGPEN 0 0 3342336) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #252=(CWidgetStyle "Terminator Label" 1 (LOGPEN 0 0 3342336) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #253=(CWidgetStyle "oriT Label" 1 (LOGPEN 0 0 13107) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #254=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #255=(CWidgetStyle "Intron" 1 (LOGPEN 0 0 16711680) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #256=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #257=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #258=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #259=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #260=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #261=(CWidgetStyle "Label 12" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16711680 1 5 "@N (@S)" 0) COMMENT #262=(CWidgetStyle "Uniq RSite" 1 (LOGPEN 0 0 16750899) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #263=(CWidgetStyle "Label 13" 1 (LOGPEN 0 0 16763904) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16711680 1 5 "@N (@S)" 0) COMMENT #264=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 39168) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32768 1 5 "@N (@S)" 0) COMMENT #265=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0 16750848) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16711680 1 5 "@N (@S)" 0) COMMENT #266=(CWidgetStyle "Marker" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #267=(CWidgetStyle "Marker Label" 1 (LOGPEN 0 0 0) 3 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #268=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 3 1 (LOGBRUSH 0 128 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #269=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 16711680) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #270=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 3381504) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #271=(CWidgetStyle "Label 16" 1 (LOGPEN 0 0 16724787) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16711680 1 5 "@N (@S)" 0) COMMENT #272=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 16737792) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #273=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0 16737792) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32768 1 5 "@N (@S)" 0) COMMENT #274=(CWidgetStyle "Label 18" 1 (LOGPEN 0 0 3381504) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32768 1 5 "@N (@S)" 0) COMMENT #275=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #276=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 10040064 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #277=(CWidgetStyle "Label 19" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #278=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 153) 3 1 COMMENT (LOGBRUSH 0 10040064 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #279=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 10040064) 4 1 COMMENT (LOGBRUSH 0 10040064 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #280=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 COMMENT (LOGBRUSH 0 10040064 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #281=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #282=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #283=(CWidgetStyle "RBS Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #284=(CWidgetStyle "Label 22" 1 (LOGPEN 0 0 3381504) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 COMMENT 5 "@N (@S)" 0) COMMENT #285=(CWidgetStyle "Label 23" 1 (LOGPEN 0 0 3381504) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32768 1 5 "@N (@S)" 0) COMMENT #286=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #287=(CWidgetStyle "Attenuator" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #288=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 6684774) 4 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #289=(CWidgetStyle "Promoter_P" 1 (LOGPEN 0 0 6684774) 8 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #290=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 6684774) 7 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #291=(CWidgetStyle "Shape 17" 1 (LOGPEN 0 0 0) 7 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #292=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #293=(CWidgetStyle "Label 24" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #294=(CWidgetStyle "Label 25" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #295=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #296=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #297=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #298=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 16763955) 1 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #299=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16750848 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #300=(CWidgetStyle "Shape 21" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16763955 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #301=(CWidgetStyle "Attenuator Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #302=(CWidgetStyle "5'UTR Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #303=(CWidgetStyle "3'UTR Label" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #304=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #305=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #306=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #307=(CWidgetStyle "tRNA Label" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #308=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #309=(CWidgetStyle "Label 31" 1 (LOGPEN 0 0 3381504) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 COMMENT 5 "@N (@S)" 0) COMMENT #310=(CWidgetStyle "Label 32" 1 (LOGPEN 0 0 16724787) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 COMMENT 1 5 "@N (@S)" 0) COMMENT #311=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 COMMENT "@N (@H%)" 0) COMMENT #312=(CWidgetStyle "Label 33" 1 (LOGPEN 0 0 3381504) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32768 1 5 "@N (@S)" 0) COMMENT #313=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16711680 1 5 "@N (@S)" 0) COMMENT #314=(CWidgetStyle "Shape 30" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16750848 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #315=(CWidgetStyle "Shape 31" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 10040064 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #316=(CWidgetStyle "Label 35" 1 (LOGPEN 0 0 3394560) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32768 1 5 "@N (@S)" 0) COMMENT #317=(CWidgetStyle "Label 36" 1 (LOGPEN 0 0 6723891) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32768 1 5 "@N (@S)" 0) COMMENT #318=(CWidgetStyle "Label 37" 1 (LOGPEN 0 0 6723891) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 8421376 1 5 "@N (@S)" 0) COMMENT #319=(CWidgetStyle "Label 38" 1 (LOGPEN 0 0 6723891) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32896 1 5 "@N (@S)" 0) COMMENT #320=(CWidgetStyle "Label 39" 1 (LOGPEN 0 0 6723891) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 65280 1 5 "@N (@S)" 0) COMMENT #321=(CWidgetStyle "Label 40" 1 (LOGPEN 0 0 6723891) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 8388736 1 5 "@N (@S)" 0) COMMENT #322=(CWidgetStyle "Label 41" 1 (LOGPEN 0 0 6723891) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16711935 1 5 "@N (@S)" 0) COMMENT #323=(CWidgetStyle "Label 42" 1 (LOGPEN 0 0 6723891) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16776960 1 5 "@N (@S)" 0) COMMENT #324=(CWidgetStyle "Label 43" 1 (LOGPEN 0 0 6723891) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 128 1 5 "@N (@S)" 0) COMMENT #325=(CWidgetStyle "Label 44" 1 (LOGPEN 0 0 39321) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32896 1 5 "@N (@S)" 0) COMMENT #326=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32896 1 5 "@N (@S)" 0) COMMENT #327=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #328=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #329=(CWidgetStyle "Label 46" 1 (LOGPEN 0 0 13311) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 COMMENT "@N (@H%)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 COMMENT (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 COMMENT (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 COMMENT (CShapeMapEntry 8 "Shape 20" 1 "Label 20") 30 COMMENT (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 COMMENT (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 COMMENT (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 COMMENT (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 COMMENT (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 COMMENT (CShapeMapEntry 5 "Line 2" 1 "Label 20") 43 COMMENT (CShapeMapEntry 0 "Line 2" 1 "Label 20") 33 COMMENT (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 COMMENT (CShapeMapEntry 0 "Line 3" 1 "Label 20") 59 COMMENT (CShapeMapEntry 0 "Shape 10" 1 "Label 20") 62 COMMENT (CShapeMapEntry 0 "Shape 1" 1 "Label 7") 52 COMMENT (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 COMMENT (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 COMMENT (CShapeMapEntry 0 "Motif" 1 "Label 46") 70 COMMENT (CShapeMapEntry 0 "Shape 23" 1 "Label 16") 68 COMMENT (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 COMMENT (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 COMMENT (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 COMMENT -11477) 1 1 0 1 1 COMMENT (mapper: 22.9746 -22.9358 40.3368 40.3368 0.01 10 -1 8432 8432 1 0 0) COMMENT #330=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 745434985 100) COMMENT (CObjectList COMMENT #331=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1162 100) COMMENT (CObjectList COMMENT #332=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #209# 46789292 0) COMMENT (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27493 COMMENT 6.27293 1 0.0214037) 0.0743974) COMMENT #333=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #206# 539782767 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 63 25 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.91 0 "pCD-F-HLH-MK-E4A-bio" "@N" 1 0 COMMENT 1.88761 0 -10 14.1806 1.46268 Nil) COMMENT #334=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #205# 2082602612 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 73 29 0 0 400 0 0 0 0 3 2 1 49 COMMENT "Courier New") 1.74233 1.05 0 "8432 bp" "@L bp" 16 COMMENT 0 0.145277 0 -10.8 5.03263 1.78497 Nil)) COMMENT (CObjectList)) COMMENT #335=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -2286 100) COMMENT (CObjectList COMMENT #336=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -1724 100) COMMENT (CObjectList COMMENT #337=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #20# 0) 1 2 0 0 #207# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 COMMENT 1.86068 2.45214 1 0.082322) 0.8 1.8 0) COMMENT #338=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 21764164 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Tn5 neomycin resistance" "@N" 1 -15.8367 COMMENT -7.41953 0 -10 10.7842 1.16519 #337#) COMMENT #339=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #24# 0) 1 2 0 0 #207# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 COMMENT 0.10118 0.741742 1 0.082322) 0.8 1.8 1) COMMENT #340=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 28249860 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "ampicillin resistance" "@N" 1 -9.52887 COMMENT 11.6509 0 -10 8.92485 1.16519 #339#) COMMENT #341=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #26# 0) 1 2 0 0 #207# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 COMMENT 3.34639 5.1855 1 0.082322) 0.8 1.8 0) COMMENT #342=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 51017340 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "hMLT mutant" COMMENT "@N" 1 14.629 -5.75356 0 -10 5.60282 1.16519 COMMENT #341#) COMMENT #343=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #32# 0) 1 2 0 0 #207# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 COMMENT 5.19666 5.55376 1 0.082322) 0.8 1.8 0) COMMENT #344=(CLabel (CWidget 0 (0 0) 1 2 0 0 #281# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "HLH dimerization domain" "@N" 1 15.6618 COMMENT 7.8023 0 -10 10.9577 1.16519 #343#)) COMMENT (CObjectList)) COMMENT #345=(CGroupWidget COMMENT (CWidget 22 (7 22 0) 1 2 0 0 Nil -1091 100) COMMENT (CObjectList COMMENT #346=(CScratch COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #280# 1168 100) COMMENT (LOGPEN 0 4 10040064) 6 1 5.57906 1.9 COMMENT 0.082322 1) COMMENT #347=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 28429612 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "FLAG epitope" COMMENT "@N" 1 11.8935 13.0928 0 -10 5.82595 1.16519 COMMENT #346#) COMMENT #348=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #280# 1362 100) COMMENT (LOGPEN 0 4 10040064) 6 1 3.31887 1.9 COMMENT 0.082322 1) COMMENT #349=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 49463852 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "bio-tag" "@N" 1 COMMENT 4.13204 -18.0837 0 -10 3.04932 1.16519 #348#)) COMMENT (CObjectList)) COMMENT #350=(CGroupWidget COMMENT (CWidget 29 (7 29 0) 1 2 0 0 Nil -670 100) COMMENT (CObjectList COMMENT #351=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #275# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) COMMENT 1.00623 5.66461 6.0991 1 0.082322) 0.8 1.8 0) COMMENT #352=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 39401404 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "hCMV-IE promoter and enhancer" "@N" 1 COMMENT 12.2447 18.408 0 -10 13.9575 1.16519 #351#) COMMENT #353=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #275# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 COMMENT 2.54365 2.75345 1 0.082322) 0.8 1.8 0) COMMENT #354=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 1096045635 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "SV40 early promoter" "@N" 1 -10.0092 COMMENT -13.4785 0 -10 8.70173 1.16519 #353#)) COMMENT (CObjectList)) COMMENT #355=(CGroupWidget COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil -459 100) COMMENT (CObjectList COMMENT #356=(CPromoterArrow COMMENT (CLine COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #226# 0 100) COMMENT (LOGPEN 0 4 128) 6 1.16464 5.54461 5.63188) COMMENT 0.4 0.082322 1.08464 1.8 1.8 0) COMMENT #357=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 1162293827 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T7 promoter" COMMENT "@N" 1 11.1301 16.149 0 -10 5.28054 1.16519 COMMENT #356#) COMMENT #358=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #25# 0) 1 2 0 0 #292# 687 100) COMMENT (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 COMMENT 1.51101 1.5735 1 0.082322) 0.8 1.8 1) COMMENT #359=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 49418724 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "lac promoter" COMMENT "@N" 1 -15.2389 0.994627 0 -10 5.45408 COMMENT 1.16519 #358#)) (CObjectList)) COMMENT #360=(CGroupWidget COMMENT (CWidget 33 (7 33 0) 1 2 0 0 Nil -1443 100) COMMENT (CObjectList COMMENT #361=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #219# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 COMMENT 2.75642 3.07559 1 0.082322) 0.8 1.8 0) COMMENT #362=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 47845044 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "f1 ori" "@N" 1 COMMENT -4.27418 -17.0445 0 -10 2.18163 1.16519 #361#) COMMENT #363=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #219# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) COMMENT 0.835356 2.51984 2.57638 1 0.082322) 0.8 1.8 COMMENT 0) COMMENT #364=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 7249792 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" COMMENT 1 -8.3534 -10.1852 0 -10 3.66911 1.16519 COMMENT #363#) COMMENT #365=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #23# 0) 1 2 0 0 #219# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 COMMENT 1.14349 1.44331 1 0.082322) 0.8 1.8 1) COMMENT #366=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 1096045635 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" COMMENT 1 -13.6557 3.97652 0 -10 3.81785 1.16519 COMMENT #365#)) (CObjectList)) COMMENT #367=(CGroupWidget COMMENT (CWidget 43 (7 43 0) 1 2 0 0 Nil -318 100) COMMENT (CObjectList COMMENT #368=(CLine COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #210# 648 100) COMMENT (LOGPEN 0 6 16711680) 8 0.835356 3.10907 COMMENT 3.27646) COMMENT #369=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 21855056 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "BGH polyA" "@N" COMMENT 1 3.39415 -19.4294 0 -10 4.58638 1.16519 COMMENT #368#) COMMENT #370=(CLine COMMENT (CWidget 0 (3 #21# 0) 1 2 0 0 #210# 989 100) COMMENT (LOGPEN 0 6 16711680) 8 0.835356 1.67617 COMMENT 1.73197) COMMENT #371=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 14352896 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "SV40 polyA early" "@N" 1 -15.8568 -2.93628 0 COMMENT -10 7.06551 1.16519 #370#) COMMENT #372=(CLine COMMENT (CWidget 0 (3 #22# 0) 1 2 0 0 #210# COMMENT 1093817128 100) (LOGPEN 0 6 16711680) 8 COMMENT 0.670712 1.67617 1.73197) COMMENT #373=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #281# 27752796 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 18 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "SV40 polyA late" "@N" 1 -16.0736 -1.51623 0 COMMENT -10 6.61927 1.16519 #372#)) (CObjectList)) COMMENT #374=(CGroupWidget COMMENT (CWidget 62 (7 62 0) 1 2 0 0 Nil -318 100) COMMENT (CObjectList COMMENT #375=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #212# 27417024 COMMENT 100) (LOGPEN 0 8 8388608) 10 1 5.55153 1.9 COMMENT 0.082322 1) COMMENT #376=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #256# 39015212 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.611111 0 "Kozak" "@N" 1 COMMENT 10.3904 10.8477 0 -10 2.10726 1.01644 #375#) COMMENT #377=(CScratch COMMENT (CWidget 0 (3 #27# 0) 1 2 0 0 #212# 14352840 COMMENT 100) (LOGPEN 0 8 8388608) 10 1 5.18326 1.9 COMMENT 0.082322 1) COMMENT #378=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #256# 21881572 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.611111 0 "Kozak" "@N" 1 COMMENT 12.7873 4.50903 0 -10 2.10726 1.01644 #377#) COMMENT #379=(CScratch COMMENT (CWidget 0 (3 #28# 0) 1 2 0 0 #212# 14352840 COMMENT 100) (LOGPEN 0 8 8388608) 10 1 3.73028 1.9 COMMENT 0.082322 1) COMMENT #380=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #256# 1178939705 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.611111 0 "E653A" "@N" 1 COMMENT 8.04021 -11.8939 0 -10 2.18163 1.01644 #379#) COMMENT #381=(CScratch COMMENT (CWidget 0 (3 #29# 0) 1 2 0 0 #212# 14352840 COMMENT 100) (LOGPEN 0 8 8388608) 10 1 3.39103 1.9 COMMENT 0.082322 1) COMMENT #382=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #256# 27689284 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.611111 0 "E806A" "@N" 1 COMMENT 4.5926 -15.9874 0 -10 2.18163 1.01644 #381#) COMMENT #383=(CScratch COMMENT (CWidget 0 (3 #30# 0) 1 2 0 0 #212# 14352840 COMMENT 100) (LOGPEN 0 8 8388608) 10 1 4.48914 1.9 COMMENT 0.082322 1) COMMENT #384=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #256# 47161156 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.611111 0 "E313A" "@N" 1 COMMENT 13.1166 -2.53466 0 -10 2.18163 1.01644 #383#) COMMENT #385=(CScratch COMMENT (CWidget 0 (3 #31# 0) 1 2 0 0 #212# 21576568 COMMENT 100) (LOGPEN 0 8 8388608) 10 1 4.48244 1.9 COMMENT 0.082322 1) COMMENT #386=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #256# 28039044 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.611111 0 "E316A" "@N" 1 COMMENT 13.0742 -3.53325 0 -10 2.18163 1.01644 #385#) COMMENT #387=(CScratch COMMENT (CWidget 0 (3 #33# 0) 1 2 0 0 #212# 14352840 COMMENT 100) (LOGPEN 0 8 8388608) 10 1 4.0807 1.9 COMMENT 0.082322 1) COMMENT #388=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #256# 1096045635 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.611111 0 COMMENT "stability creator" "@N" 1 12.9827 -7.99866 0 COMMENT -10 5.05742 1.01644 #387#) COMMENT #389=(CScratch COMMENT (CWidget 0 (3 #34# 0) 1 2 0 0 #212# COMMENT 2082602612 100) (LOGPEN 0 8 8388608) 10 1 COMMENT 3.73698 1.9 0.082322 1) COMMENT #390=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #256# 909588787 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.611111 0 "PCR artefact" COMMENT "@N" 1 9.41072 -10.8953 0 -10 4.36326 1.01644 COMMENT #389#)) (CObjectList))) (CObjectList)) COMMENT #391=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1302 100) COMMENT (CObjectList COMMENT #392=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -1021 100) COMMENT (CObjectList COMMENT #393=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #328# 12 100) COMMENT (LOGPEN 0 0 16724736) 1 1 3.72731 1.9 COMMENT 0.082322 1) COMMENT #394=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #271# 539782767 100) COMMENT (LOGPEN 0 0 16724787) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 16711680 COMMENT "{\\i Age}I (3423)" "@N (@S)" 5 8.55461 COMMENT -12.8429 0 -10 3.84264 0.942068 #393#)) COMMENT (CObjectList)) COMMENT #395=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1373 100) COMMENT (CObjectList COMMENT #396=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #328# 14352840 COMMENT 100) (LOGPEN 0 0 16724736) 1 1 4.74283 1.9 COMMENT 0.082322 1) COMMENT #397=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #271# 1096045635 100) COMMENT (LOGPEN 0 0 16724787) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 16711680 COMMENT "{\\i Eco}RI (2058)" "@N (@S)" 5 14.2264 COMMENT -0.271715 0 -10 4.38805 0.942068 #396#)) COMMENT (CObjectList)) COMMENT #398=(CGroupWidget COMMENT (CWidget 1 (10 #9# 0) 1 2 0 0 Nil -248 100) COMMENT (CObjectList COMMENT #399=(CScratch COMMENT (CWidget 1 (1 #9# 1) 1 2 0 0 #328# 14352840 COMMENT 100) (LOGPEN 0 0 16724736) 1 1 5.43398 1.9 COMMENT 0.082322 1) COMMENT #400=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #271# 27989728 100) COMMENT (LOGPEN 0 0 16724787) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 16711680 COMMENT "{\\i Xho}I (1129)" "@N (@S)" 5 11.5756 COMMENT 9.79949 0 -10 3.91702 0.942068 #399#)) COMMENT (CObjectList)) COMMENT #401=(CGroupWidget COMMENT (CWidget 2 (10 #2# 0) 1 2 0 0 Nil -1232 100) COMMENT (CObjectList COMMENT #402=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #327# 1168 100) COMMENT (LOGPEN 0 0 39321) 1 1 5.38488 1.9 0.082322 1) COMMENT #403=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 1128350529 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Bst}XI (1195)" "@N (@S)" 5 11.9101 COMMENT 8.82569 0 -10 4.23931 0.942068 #402#) COMMENT #404=(CScratch COMMENT (CWidget 2 (1 #2# 2) 1 2 0 0 #327# 1362 100) COMMENT (LOGPEN 0 0 39321) 1 1 4.0621 1.9 0.082322 1) COMMENT #405=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 1129595719 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Bst}XI (2973)" "@N (@S)" 5 12.0217 COMMENT -9.87189 0 -10 4.23931 0.942068 #404#)) COMMENT (CObjectList)) COMMENT #406=(CGroupWidget COMMENT (CWidget 2 (10 #4# 0) 1 2 0 0 Nil -1583 100) COMMENT (CObjectList COMMENT #407=(CScratch COMMENT (CWidget 1 (1 #4# 1) 1 2 0 0 #327# 16 100) COMMENT (LOGPEN 0 0 39321) 1 1 5.59543 1.9 0.082322 1) COMMENT #408=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 28249860 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Hin}dIII (912)" "@N (@S)" 5 10.8551 COMMENT 15.0473 0 -10 4.31368 0.942068 #407#) COMMENT #409=(CScratch COMMENT (CWidget 2 (1 #4# 2) 1 2 0 0 #327# 14352840 COMMENT 100) (LOGPEN 0 0 39321) 1 1 4.07698 1.9 COMMENT 0.082322 1) COMMENT #410=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 1096045635 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Hin}dIII (2953)" "@N (@S)" 5 12.556 COMMENT -8.82371 0 -10 4.68555 0.942068 #409#)) COMMENT (CObjectList)) COMMENT #411=(CGroupWidget COMMENT (CWidget 2 (10 #6# 0) 1 2 0 0 Nil -1021 100) COMMENT (CObjectList COMMENT #412=(CScratch COMMENT (CWidget 1 (1 #6# 1) 1 2 0 0 #327# 6 100) COMMENT (LOGPEN 0 0 39321) 1 1 6.11844 1.9 0.082322 1) COMMENT #413=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 45173864 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nru}I (209)" "@N (@S)" 5 4.24756 COMMENT 19.5801 0 -10 3.52036 0.942068 #412#) COMMENT #414=(CScratch COMMENT (CWidget 2 (1 #6# 2) 1 2 0 0 #327# 2082602612 COMMENT 100) (LOGPEN 0 0 39321) 1 1 3.38806 1.9 COMMENT 0.082322 1) COMMENT #415=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 909326647 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nru}I (3879)" "@N (@S)" 5 5.17208 COMMENT -16.8868 0 -10 3.91702 0.942068 #414#)) COMMENT (CObjectList)) COMMENT #416=(CGroupWidget COMMENT (CWidget 3 (10 #1# 0) 1 2 0 0 Nil -881 100) COMMENT (CObjectList COMMENT #417=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #327# 7 100) COMMENT (LOGPEN 0 0 39321) 1 1 6.24938 1.9 0.082322 1) COMMENT #418=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 656941088 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Alw}44I (33)" "@N (@S)" 5 2.40925 COMMENT 20.7275 0 -10 3.91702 0.942068 #417#) COMMENT #419=(CScratch COMMENT (CWidget 2 (1 #1# 2) 1 2 0 0 #327# 1298 100) COMMENT (LOGPEN 0 0 39321) 1 1 1.11745 1.9 0.082322 1) COMMENT #420=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 38820268 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Alw}44I (6931)" "@N (@S)" 5 -12.8111 COMMENT 5.5701 0 -10 4.68555 0.942068 #419#) COMMENT #421=(CScratch COMMENT (CWidget 3 (1 #1# 3) 1 2 0 0 #327# 1104 100) COMMENT (LOGPEN 0 0 39321) 1 1 0.190457 1.9 0.082322 COMMENT 1) COMMENT #422=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 27738436 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Alw}44I (8177)" "@N (@S)" 5 -5.02129 COMMENT 13.8216 0 -10 4.68555 0.942068 #421#)) COMMENT (CObjectList)) COMMENT #423=(CGroupWidget COMMENT (CWidget 3 (10 #7# 0) 1 2 0 0 Nil -2216 100) COMMENT (CObjectList COMMENT #424=(CScratch COMMENT (CWidget 1 (1 #7# 1) 1 2 0 0 #327# 2082602612 COMMENT 100) (LOGPEN 0 0 39321) 1 1 4.9385 1.9 COMMENT 0.082322 1) COMMENT #425=(CLabel (CWidget 0 (0 0) 1 2 0 0 #326# 0 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Pst}I (1795)" "@N (@S)" 5 13.7532 COMMENT 3.16336 0 -10 3.6939 0.942068 #424#) COMMENT #426=(CScratch COMMENT (CWidget 2 (1 #7# 2) 1 2 0 0 #327# 14352840 COMMENT 100) (LOGPEN 0 0 39321) 1 1 3.53164 1.9 COMMENT 0.082322 1) COMMENT #427=(CLabel (CWidget 0 (0 0) 1 2 0 0 #326# 0 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Pst}I (3686)" "@N (@S)" 5 6.71519 COMMENT -13.9654 0 -10 3.6939 0.942068 #426#) COMMENT #428=(CScratch COMMENT (CWidget 3 (1 #7# 3) 1 2 0 0 #327# 14352840 COMMENT 100) (LOGPEN 0 0 39321) 1 1 2.31673 1.9 COMMENT 0.082322 1) COMMENT #429=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 21764164 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Pst}I (5319)" "@N (@S)" 5 -10.4528 COMMENT -8.69082 0 -10 3.6939 0.942068 #428#)) COMMENT (CObjectList)) COMMENT #430=(CGroupWidget COMMENT (CWidget 5 (10 #8# 0) 1 2 0 0 Nil -1232 100) COMMENT (CObjectList COMMENT #431=(CScratch COMMENT (CWidget 1 (1 #8# 1) 1 2 0 0 #327# 6 100) COMMENT (LOGPEN 0 0 39321) 1 1 4.47872 1.9 0.082322 1) COMMENT #432=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 39401404 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Sph}I (2413)" "@N (@S)" 5 13.8787 COMMENT -4.48226 0 -10 3.84264 0.942068 #431#) COMMENT #433=(CScratch COMMENT (CWidget 2 (1 #8# 2) 1 2 0 0 #327# 7249792 COMMENT 100) (LOGPEN 0 0 39321) 1 1 3.12692 1.9 COMMENT 0.082322 1) COMMENT #434=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 44367748 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Sph}I (4230)" "@N (@S)" 5 -2.52139 COMMENT -18.1918 0 -10 3.84264 0.942068 #433#) COMMENT #435=(CScratch COMMENT (CWidget 3 (1 #8# 3) 1 2 0 0 #327# 14352840 COMMENT 100) (LOGPEN 0 0 39321) 1 1 2.69988 1.9 COMMENT 0.082322 1) COMMENT #436=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 44367220 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Sph}I (4804)" "@N (@S)" 5 -7.32733 COMMENT -14.6011 0 -10 3.84264 0.942068 #435#) COMMENT #437=(CScratch COMMENT (CWidget 4 (1 #8# 4) 1 2 0 0 #327# 1362 100) COMMENT (LOGPEN 0 0 39321) 1 1 2.64631 1.9 0.082322 1) COMMENT #438=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 39009076 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Sph}I (4876)" "@N (@S)" 5 -7.82682 COMMENT -12.4055 0 -10 3.84264 0.942068 #437#) COMMENT #439=(CScratch COMMENT (CWidget 5 (1 #8# 5) 1 2 0 0 #327# 8 100) COMMENT (LOGPEN 0 0 39321) 1 1 2.05486 1.9 0.082322 1) COMMENT #440=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 47845044 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Sph}I (5671)" "@N (@S)" 5 -12.9466 COMMENT -6.27219 0 -10 3.84264 0.942068 #439#)) COMMENT (CObjectList)) COMMENT #441=(CGroupWidget COMMENT (CWidget 5 (10 #10# 0) 1 2 0 0 Nil -459 100) COMMENT (CObjectList COMMENT #442=(CScratch COMMENT (CWidget 1 (1 #10# 1) 1 2 0 0 #327# 38396760 COMMENT 100) (LOGPEN 0 0 39321) 1 1 5.25989 1.9 COMMENT 0.082322 1) COMMENT #443=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 51801596 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Xmn}I (1363)" "@N (@S)" 5 12.5822 COMMENT 6.58059 0 -10 4.06577 0.942068 #442#) COMMENT #444=(CScratch COMMENT (CWidget 2 (1 #10# 2) 1 2 0 0 #327# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 4.92287 1.9 0.082322 1) COMMENT #445=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 44359652 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Xmn}I (1816)" "@N (@S)" 5 14.1521 COMMENT 1.94165 0 -10 4.06577 0.942068 #444#) COMMENT #446=(CScratch COMMENT (CWidget 3 (1 #10# 3) 1 2 0 0 #327# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 4.24288 1.9 0.082322 1) COMMENT #447=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 1096045635 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Xmn}I (2730)" "@N (@S)" 5 13.5419 COMMENT -6.95048 0 -10 4.06577 0.942068 #446#) COMMENT #448=(CScratch COMMENT (CWidget 4 (1 #10# 4) 1 2 0 0 #327# 14352840 COMMENT 100) (LOGPEN 0 0 39321) 1 1 2.75642 1.9 COMMENT 0.082322 1) COMMENT #449=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 539782767 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Xmn}I (4728)" "@N (@S)" 5 -7.11181 COMMENT -15.6988 0 -10 4.06577 0.942068 #448#) COMMENT #450=(CScratch COMMENT (CWidget 5 (1 #10# 5) 1 2 0 0 #327# 14352840 COMMENT 100) (LOGPEN 0 0 39321) 1 1 0.241047 1.9 COMMENT 0.082322 1) COMMENT #451=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 808732464 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Xmn}I (8109)" "@N (@S)" 5 -5.14421 COMMENT 12.7735 0 -10 4.06577 0.942068 #450#)) COMMENT (CObjectList)) COMMENT #452=(CGroupWidget COMMENT (CWidget 7 (10 #5# 0) 1 2 0 0 Nil -1583 100) COMMENT (CObjectList COMMENT #453=(CScratch COMMENT (CWidget 1 (1 #5# 1) 1 2 0 0 #327# 14352840 COMMENT 100) (LOGPEN 0 0 39321) 1 1 5.81936 1.9 COMMENT 0.082322 1) COMMENT #454=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 268673032 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nco}I (611)" "@N (@S)" 5 7.90117 COMMENT 17.3598 0 -10 3.52036 0.942068 #453#) COMMENT #455=(CScratch COMMENT (CWidget 2 (1 #5# 2) 1 2 0 0 #327# 14352840 COMMENT 100) (LOGPEN 0 0 39321) 1 1 5.59022 1.9 COMMENT 0.082322 1) COMMENT #456=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 44367484 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nco}I (919)" "@N (@S)" 5 10.678 14.1727 COMMENT 0 -10 3.52036 0.942068 #455#) COMMENT #457=(CScratch COMMENT (CWidget 3 (1 #5# 3) 1 2 0 0 #327# 12 100) COMMENT (LOGPEN 0 0 39321) 1 1 5.55451 1.9 0.082322 1) COMMENT #458=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 45605224 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nco}I (967)" "@N (@S)" 5 11.0322 COMMENT 11.9702 0 -10 3.52036 0.942068 #457#) COMMENT #459=(CScratch COMMENT (CWidget 4 (1 #5# 4) 1 2 0 0 #327# 7 100) COMMENT (LOGPEN 0 0 39321) 1 1 5.18624 1.9 0.082322 1) COMMENT #460=(CLabel (CWidget 0 (0 0) 1 2 0 0 #326# 12 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nco}I (1462)" "@N (@S)" 5 13.5818 COMMENT 5.63158 0 -10 3.91702 0.942068 #459#) COMMENT #461=(CScratch COMMENT (CWidget 5 (1 #5# 5) 1 2 0 0 #327# 26891680 COMMENT 100) (LOGPEN 0 0 39321) 1 1 3.49593 1.9 COMMENT 0.082322 1) COMMENT #462=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 38461396 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nco}I (3734)" "@N (@S)" 5 6.58317 COMMENT -14.9392 0 -10 3.91702 0.942068 #461#) COMMENT #463=(CScratch COMMENT (CWidget 6 (1 #5# 6) 1 2 0 0 #327# 3 100) COMMENT (LOGPEN 0 0 39321) 1 1 2.58159 1.9 0.082322 1) COMMENT #464=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #326# 14352952 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nco}I (4963)" "@N (@S)" 5 -8.27058 COMMENT -11.407 0 -10 3.91702 0.942068 #463#) COMMENT #465=(CScratch COMMENT (CWidget 7 (1 #5# 7) 1 2 0 0 #327# 2082602612 COMMENT 100) (LOGPEN 0 0 39321) 1 1 2.03477 1.9 COMMENT 0.082322 1) COMMENT #466=(CLabel (CWidget 0 (0 0) 1 2 0 0 #326# 0 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nco}I (5698)" "@N (@S)" 5 -13.2118 COMMENT -5.14964 0 -10 3.91702 0.942068 #465#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #467=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -108 100) COMMENT (CObjectList COMMENT #468=(CLabel (CWidget 0 (15 #35# 0) 1 2 0 0 #232# 7 100) COMMENT (LOGPEN 1769105217 3421728 34079223) 2082602612 COMMENT (LOGFONT 63 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.91 0 "LMBP 5683" COMMENT "LMBP 5683" 65535 0 -1.46268 0 -10 7.80925 1.56185 COMMENT Nil) COMMENT #469=(CLabel COMMENT (CWidget 0 (15 #36# 0) 1 2 0 0 #232# 842614085 100) COMMENT (LOGPEN 1128547885 926168888 944064327) 858604345 COMMENT (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.611111 0 "Last modified: 27-JUL-2009" COMMENT "Last modified: 27-JUL-2009" 65535 0 -3.02453 0 -10 COMMENT 8.99923 1.01644 Nil)) (CObjectList)) COMMENT #470=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -2427 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 COMMENT (CStringList) 1094795585 1195852099 (CObList))) FEATURES Location/Qualifiers misc_marker 923..946 /vntifkey="22" /label=FLAG\epitope promoter 235..818 /vntifkey="29" /label=hCMV-IE\promoter\and\enhancer promoter 863..879 /vntifkey="30" /label=T7\promoter misc_marker 3950..3994 /vntifkey="22" /label=bio-tag mutation 971..973 /vntifkey="62" /label=Kozak /note="TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG" mutation 3410..3412 /vntifkey="62" /label=PCR\artefact /note="silent mutation; wt: ACA" mutation 2948..2950 /vntifkey="62" /label=stability\creator /note="silent mutation; wt: GCA ('GCA'GAAG recombines with the same heptamer in hAPI2)" terminator 4029..4253 /vntifkey="43" /label=BGH\polyA rep_origin 4299..4727 /vntifkey="33" /label=f1\ori rep_origin 4970..5045 /vntifkey="33" /label=SV40\ori promoter 4732..5013 /vntifkey="29" /label=SV40\early\promoter CDS 5137..5931 /vntifkey="4" /label=Tn5\neomycin\resistance terminator 6105..6179 /vntifkey="43" /label=SV40\polyA\early terminator complement(6105..6179) /vntifkey="43" /label=SV40\polyA\late rep_origin complement(6493..6895) /vntifkey="33" /label=pMB1\ori CDS complement(7436..8296) /vntifkey="4" /label=ampicillin\resistance promoter complement(6318..6401) /vntifkey="30" /label=lac\promoter CDS 968..1447 /vntifkey="4" /label=HLH\dimerization\domain /note="(hMLT: 968-1093; hETV6: 1094-1447)" CDS 1463..3934 /vntifkey="4" /label=hMLT\mutant mutation 1466..1468 /vntifkey="62" /label=Kozak /note="TCG was replaced by GCG (S2A) creating Kozak consensus sequence GCCACCATGG" mutation 3419..3427 /vntifkey="62" /label=E653A /note="wt: GAA.ACT.GGC" mutation 3875..3880 /vntifkey="62" /label=E806A /note="wt: GTA.GAG" mutation 2399..2401 /vntifkey="62" /label=E313A /note="wt: GAG" mutation 2408..2410 /vntifkey="62" /label=E316A /note="wt: GAG" BASE COUNT 2079 a 2087 c 2110 g 2156 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc 961 tgccaccatg gcgctgttgg gggacccgct acaggccctg ccgccctcgg ccgcccccac 1021 ggggccgctg ctcgcccctc cggccggcgc gaccctcaac cgcctgcggg agccgctgct 1081 gcggaggctc agctacgctt cctcgacgcc acttcatgtt ccagtgcctc gagcgctcag 1141 gatggaggaa gactcgatcc gcctgcctgc gcacctgcgc ttgcagccaa tttactggag 1201 cagggatgac gtagcccagt ggctcaagtg ggctgaaaat gagttttctt taaggccaat 1261 tgacagcaac acgtttgaaa tgaatggcaa agctctcctg ctgctgacca aagaggactt 1321 tcgctatcga tctcctcatt caggtgatgt gctctatgaa ctccttcagc atattctgaa 1381 gcagaggaaa cctcggattc ttttttcacc attcttccac cctggaaact ctatacacac 1441 acagccggtt gatcctgcca ccatggcgct gttgggggac ccgctacagg ccctgccgcc 1501 ctcggccgcc cccacggggc cgctgctcgc ccctccggcc ggcgcgaccc tcaaccgcct 1561 gcgggagccg ctgctgcgga ggctcagcga gctcctggat caggcgcccg agggccgggg 1621 ctggaggaga ctggcggagc tggcggggag tcgcgggcgc ctccgcctca gttgcctaga 1681 cctggagcag tgttctctta aggtactgga gcctgaagga agccccagcc tgtgtctgct 1741 gaagttaatg ggtgaaaaag gttgcacagt cacagaattg agtgatttcc tgcaggctat 1801 ggaacacact gaagttcttc agcttctcag ccccccagga ataaagatta ctgtaaaccc 1861 agagtcaaag gcagtcttgg ctggacagtt tgtgaaactg tgttgccggg caactggaca 1921 tccttttgtt caatatcagt ggttcaaaat gaataaagag attccaaatg gaaatacatc 1981 agagcttatt tttaatgcag tgcatgtaaa agatgcaggc ttttatgtct gtcgagttaa 2041 taacaatttc acctttgaat tcagccagtg gtcacagctg gatgtttgcg acatcccaga 2101 gagcttccag agaagtgttg atggcgtctc tgaatccaag ttgcaaatct gtgttgaacc 2161 aacttcccaa aagctgatgc caggcagcac attggtttta cagtgtgttg ctgttggaag 2221 ccctattcct cactaccagt ggttcaaaaa tgaattacca ttaacacatg agaccaaaaa 2281 gctatacatg gtgccttatg tggatttgga acaccaagga acctactggt gtcatgtata 2341 taatgatcga gacagtcaag atagcaagaa ggtagaaatc atcataggaa gaacagatgc 2401 ggcagtggca tgcactgaag atgaattaaa taatcttggt catcctgata ataaagagca 2461 aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa atatgaatta 2521 ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga ctaacttact 2581 gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg agatgcgtaa 2641 tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat tatattatgc 2701 aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg ctccaaatcc 2761 atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc aagaaaaaga 2821 aactggactt aatgtgttct tattggatat gtgtaggaaa agaaatgact acgatgatac 2881 cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat atgccacgtg 2941 tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa tctttatgaa 3001 atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg atgaagttgc 3061 agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga ttcgaagtag 3121 tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt ctgctgaatc 3181 tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta tgtgtcttaa 3241 gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca atgtcatgat 3301 catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg cctacgttac 3361 tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca cgcctgaagc 3421 aaccggtagc tacttggtat caaaggatct tcccaagcat tgcctctata ccagactcag 3481 ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat atcagtactc 3541 aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac ctctcattgc 3601 taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt gtcttatgtc 3661 taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt atcactcatt 3721 gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta atgttacacc 3781 agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt tcgctcacca 3841 tgcttcatgt cattttagta gaagtaatgt gccagtcgcg acaactgatg aaataccatt 3901 tagtttctct gacaggctca gaatttctga aaaagggccc gtttccggag gcctgaacga 3961 catcttcgag gcccagaaga tcgagtggca cgagtaaggg cccgtttaaa cccgctgatc 4021 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 4081 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 4141 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 4201 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 4261 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 4321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 4381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 4441 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 4501 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 4561 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 4621 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 4681 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 4741 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4801 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 4861 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 4921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 4981 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 5041 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 5101 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 5161 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 5221 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 5281 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 5341 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 5401 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 5461 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 5521 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 5581 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 5641 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 5701 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 5761 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 5821 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 5881 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 5941 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 6001 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 6061 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 6121 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 6181 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 6241 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 6301 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6361 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6421 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 6481 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 6541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 6721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 6781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 6841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 6901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 6961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 7141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 7261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 7321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 7381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 7441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 7501 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 7561 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 7621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 7681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 7741 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 7801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 7861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 7921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 7981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8041 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 8101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 8161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 8221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 8281 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 8341 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 8401 gcacatttcc ccgaaaagtg ccacctgacg tc //