LOCUS       pCD-F-MLTp10p20        6212 bp    DNA     circular     11-FEB-2008
DEFINITION  LMBP 5684.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|473785290|
COMMENT     VNTDBDATE|473785290|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCD-F-MLTp10p20|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     promoter        2979..3633
                     /vntifkey="29"
                     /label=pCMV
     promoter        3633..3652
                     /vntifkey="30"
                     /label=T7
     polyA_signal    285..524
                     /vntifkey="25"
                     /label=SV40\polyA
     rep_origin      956..1629
                     /vntifkey="33"
                     /label=ColE1
     misc_marker     complement(1774..2635)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    3690..3716
                     /vntifkey="21"
                     /label=flag\epitope
     primer          3633..3652
                     /vntifkey="27"
                     /label=2506
     primer          4488..4508
                     /vntifkey="27"
                     /label=1726
     primer          4405..4425
                     /vntifkey="27"
                     /label=1725
     primer          complement(4569..4585)
                     /vntifkey="27"
                     /label=1503
     promoter        5326..5651
                     /vntifkey="29"
                     /label=SV40
     rep_origin      4848..5261
                     /vntifkey="33"
                     /label=f1
     polyA_signal    4571..4785
                     /vntifkey="25"
                     /label=BGH\polyA
     misc_recomb     5687..269
                     /vntifkey="86"
                     /label=NeoR
BASE COUNT     1510 a      1534 c      1565 g      1603 t 
ORIGIN
        1 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
       61 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
      121 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
      181 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
      241 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 
      301 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 
      361 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
      421 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 
      481 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
      541 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 
      601 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
      661 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 
      721 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
      781 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
      841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
      901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
      961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     1021 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     1081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     1141 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     1201 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     1261 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     1321 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     1381 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     1441 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     1501 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     1561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     1621 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     1681 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     1741 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     1801 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     1861 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     1921 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     1981 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     2041 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     2101 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     2161 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     2221 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     2281 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     2341 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     2401 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     2461 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     2521 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     2581 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     2641 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     2701 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     2761 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc 
     2821 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct 
     2881 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg 
     2941 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg 
     3001 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat 
     3061 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     3121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 
     3181 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta 
     3241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 
     3301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 
     3361 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 
     3421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 
     3481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 
     3541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact 
     3601 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa 
     3661 gctggctagc gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac 
     3721 ctaaggatcc agtgtggtgg aattctgcag atagaaatga ctacgatgat accattccaa 
     3781 tcttggatgc actaaaagtc accgccaata ttgtgtttgg atatgccacg tgtcaaggag 
     3841 ctgaagcttt tgaaatccag cattctggat tggcaaatgg aatctttatg aaatttttaa 
     3901 aagacagatt attagaagat aagaaaatca ctgtgttact ggatgaagtt gcagaagata 
     3961 tgggtaagtg tcaccttacc aaaggcaaac aggctctaga gattcgaagt agtttatctg 
     4021 agaagagaaa tcttggtcat cctgataata aagagcaaac aactgaccag cctttggcga 
     4081 aggacaaggt tgcccttttg ataggaaata tgaattaccg ggagcacccc aagctcaaag 
     4141 ctcctttggt ggatgtgtac gaattgacta acttactgag acagctggac ttcaaagtgg 
     4201 tttcactgtt ggatcttact gaatatgaga tgcgtaatgc tgtggatgag tttttactcc 
     4261 ttttagacaa gggagtatat gggttattat attatgcagg acatggttat gaaaattttg 
     4321 ggaacagctt catggtcccc gttgatgctc caaatccata taggtctgaa aattgtctgt 
     4381 gtgtacaaaa tatactgaaa ttgatgcaag aaaaagaaac tggacttaat gtgttcttat 
     4441 tggatatgtg taggaaaaga aatgactacg atgataccat tccaatcttg gatgcactaa 
     4501 aagtcaccgc caatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac 
     4561 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 
     4621 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 
     4681 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 
     4741 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 
     4801 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 
     4861 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     4921 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     4981 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca 
     5041 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     5101 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     5161 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg 
     5221 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 
     5281 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag 
     5341 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     5401 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     5461 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     5521 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 
     5581 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 
     5641 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 
     5701 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 
     5761 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 
     5821 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 
     5881 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 
     5941 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 
     6001 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 
     6061 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 
     6121 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 
     6181 cgcgccagcc gaactgttcg ccaggctcaa gg 
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